Polyvinyl alcohol( PVA) is a water-soluble synthetic polymer that is hard to biodegrade. PVA-degrading microorganisms were previously reported as unitary bacteria and most of them have been identified as aerobes. In t...Polyvinyl alcohol( PVA) is a water-soluble synthetic polymer that is hard to biodegrade. PVA-degrading microorganisms were previously reported as unitary bacteria and most of them have been identified as aerobes. In this work,a microbial community was cultured anaerobically and its degradation performance and biodiversity were analyzed. The microbial community was cultured for more than 40 d,which represents a highly efficient degradation performance with a chemical oxygen demand removal efficiency of 88. 48%. Operational taxonomic unit-based analysis of the sequences revealed a highly diverse community in the reactor. To note,metagenome 16s rDNA sequencing delineated 19 phyla and 41 classes. Specifically, proteobacteria, chlamydiae, bacteroidetes,firmicutes,and planctomycetes play key roles in the biodegradation processes. Moreover,the betaproteobacteria class belonging to the proteobacteria phylum was the predominant bacterial members in this community. Our results demonstrated that anaerobic treatment of PVA wastewater is feasible and confers degradation by a highly diverse microbial community.展开更多
从油水淹地污染土壤中获得26株石油降解菌,筛选出表面活性剂产生菌1株(H-6)和优势菌6株(H-1、H-17、H-18、H-19、H-20、H-23),结合菌株形态观察、革兰氏染色和16S r DNA序列同源性分析,鉴定表面活性剂产生菌H-6为堀越氏芽孢杆菌(Bacillu...从油水淹地污染土壤中获得26株石油降解菌,筛选出表面活性剂产生菌1株(H-6)和优势菌6株(H-1、H-17、H-18、H-19、H-20、H-23),结合菌株形态观察、革兰氏染色和16S r DNA序列同源性分析,鉴定表面活性剂产生菌H-6为堀越氏芽孢杆菌(Bacillus horikoshii)。以表面活性剂产生菌H-6为中心,再任选3株优势菌株构建石油降解菌群,得到高效石油降解菌群C5(H-1、H-6、H-18、H-19),通过正交试验得到菌群C5各菌种的最佳接种量。结果表明:接种量15%(H-1)、15%(H-6)、20%(H-18)、10%(H-19),温度25℃,石油含量2 000 mg/L条件下,7 d时石油降解率达到78.87%。这说明高效降解菌群C5对石油具有较好的降解效果,可应用于油水淹地污染土壤的修复。展开更多
基金Science and Technology Plan of Henan Province,China(No.132300410080)North China University of Water Resources and Electric Power Postgraduate Education Innovation Program,China(No.YK2015-13)
文摘Polyvinyl alcohol( PVA) is a water-soluble synthetic polymer that is hard to biodegrade. PVA-degrading microorganisms were previously reported as unitary bacteria and most of them have been identified as aerobes. In this work,a microbial community was cultured anaerobically and its degradation performance and biodiversity were analyzed. The microbial community was cultured for more than 40 d,which represents a highly efficient degradation performance with a chemical oxygen demand removal efficiency of 88. 48%. Operational taxonomic unit-based analysis of the sequences revealed a highly diverse community in the reactor. To note,metagenome 16s rDNA sequencing delineated 19 phyla and 41 classes. Specifically, proteobacteria, chlamydiae, bacteroidetes,firmicutes,and planctomycetes play key roles in the biodegradation processes. Moreover,the betaproteobacteria class belonging to the proteobacteria phylum was the predominant bacterial members in this community. Our results demonstrated that anaerobic treatment of PVA wastewater is feasible and confers degradation by a highly diverse microbial community.
