Background:The aim of this work is to detect and compare the peripheral blood mi RNA expression profiles in patients with severe traumatic brain injury(s TBI)2,12,24,48,and 72 h after injury at high altitude and to pr...Background:The aim of this work is to detect and compare the peripheral blood mi RNA expression profiles in patients with severe traumatic brain injury(s TBI)2,12,24,48,and 72 h after injury at high altitude and to predict the target genes of differential expressed mi RNAs.Methods:Twenty s TBI patients from high-altitude areas were randomly selected according to the inclusion and exclusion criteria and were divided into five groups:the 2-h group,12-h group,24-h group,48-h group,and 72-h group.Peripheral blood mi RNA expression profiles were detected using real-time quantitative PCR(q RT-PCR).Results:The expression levels of mi R-18 a,mi R-203,mi R-146 a,mi R-149,mi R-23 b,and mi R-let-7 b in peripheral blood showed significant differences between the 2-h group and the 12-h group.The expression levels of mi R-203,mi R-146 a,mi R-149,mi R-23 b,and mi R-let-7 f in peripheral blood were up-regulated in the 24-h group.In the 48-h group,the expression levels of mi R-181 d,mi R-29 a,and mi R-18 b were upregulated.In the 72-h group,the expression levels of mi R-203,mi R-146 a,mi R-149,mi R-23 b,and mi R-let-7 f changed.The main target genes of the differentiation expressed mi RNAs were genes that regulate inflammatory responses,apoptosis,and DNA damage/repair.Conclusions:mi RNAs may be involved in the pathogenesis of s TBI by dynamically regulating the target genes that regulate inflammatory responses,apoptosis,and DNA damage/repair pathways.展开更多
62 patients with traumatic paraplegia were treated with acupuncture(GovernorVessel electro-stimulation),according to the theories of TCM such as dredging the meridians,regu-lating vital energy and blood,improving bloo...62 patients with traumatic paraplegia were treated with acupuncture(GovernorVessel electro-stimulation),according to the theories of TCM such as dredging the meridians,regu-lating vital energy and blood,improving blood circulation,reinforcing marrow and replenishing brain.The effect of acupuncture on urinary bladder disturbance was studied.The results indicated that totaleffect rate was 96.8%(Ⅰ—Ⅲ),marked effect rate was 66.1%(Ⅰ—Ⅱ).The marked effect rate incomplete paraplegia was significantly different from that in imcomplete paraplegia;it was 63.0% and87.5% respectively.Furthermore,the results suggested that the degree of spinal cord injury was veryimprotant factor that affected the effect of acupuncture therapy.展开更多
Neuroinflammation and the NACHT,LRR,and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury(TBI).Maraviroc,a ...Neuroinflammation and the NACHT,LRR,and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury(TBI).Maraviroc,a C-C chemokine receptor type 5 antagonist,has been viewed as a new therapeutic strategy for many neuroinflammatory diseases.We studied the effect of maraviroc on TBI-induced neuroinflammation.A moderate-TBI mouse model was subjected to a controlled cortical impact device.Maraviroc or vehicle was injected intraperitoneally 1 hour after TBI and then once per day for 3 consecutive days.Western blot,immunohistochemistry,and TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling)analyses were performed to evaluate the molecular mechanisms of maraviroc at 3 days post-TBI.Our results suggest that maraviroc administration reduced NACHT,LRR,and PYD domains-containing protein 3 inflammasome activation,modulated microglial polarization from M1 to M2,decreased neutrophil and macrophage infiltration,and inhibited the release of inflammatory factors after TBI.Moreover,maraviroc treatment decreased the activation of neurotoxic reactive astrocytes,which,in turn,exacerbated neuronal cell death.Additionally,we confirmed the neuroprotective effect of maraviroc using the modified neurological severity score,rotarod test,Morris water maze test,and lesion volume measurements.In summary,our findings indicate that maraviroc might be a desirable pharmacotherapeutic strategy for TBI,and C-C chemokine receptor type 5 might be a promising pharmacotherapeutic target to improve recovery after TBI.展开更多
Traumatic axonal injury is a progressive process evoked by shear forces on the brain, gradually evolving from focal axonal alteration and cumulating in neural disconnection. Clinical classifiers and conventional neuro...Traumatic axonal injury is a progressive process evoked by shear forces on the brain, gradually evolving from focal axonal alteration and cumulating in neural disconnection. Clinical classifiers and conventional neuroimaging are limited in traumatic axonal injury detection, outcome prediction, and treatment guidance. Diffusion weighted imaging is an advanced magnetic resonance imaging (MRI) technique that is sensitive to the movement of water molecules, providing additional information on the micro-structural arrangement of tissue. Quantitative analysis of diffusion metrics can aid in the localization of axonal injury and/or de(dys)myelination caused by trauma. Diffusion MRI tractography is an extension of diffusion weighted imaging, and can provide additional information about white matter pathways and the integrity of brain neural networks. Both techniques are able to detect the early micro-structural changes caused by Traumatic Brain Injury (TBI), and can be used to increase understanding of the mechanisms of brain plasticity in recovery after brain injury and possibly optimize treatment planning of patients with Traumatic Brain Injury. This review focuses on the theoretical basis and applied advanced techniques of diffusion weighted imaging, their limitations and applications, and future directions in the application to TBI.展开更多
Sepsis and subsequent multiple organ dysfunction syndrome(MODS) are frequent complications after severe traumata or burns involving a large area,and these remain as the two most common causes of morbidity and mortalit...Sepsis and subsequent multiple organ dysfunction syndrome(MODS) are frequent complications after severe traumata or burns involving a large area,and these remain as the two most common causes of morbidity and mortality in critical illnesses.Despite the recent rapid advances in intensive展开更多
目的:观察骨髓间质干细胞(bone m arrow strom a cells,BM SCs)和脑源性神经营养因子(brain derived neurotrophic factor,BD N F)联合应用对兔脊髓外伤性截瘫结构和功能修复的影响,以寻求建立一种更有效的治疗脊髓外伤性截瘫的疗法。方...目的:观察骨髓间质干细胞(bone m arrow strom a cells,BM SCs)和脑源性神经营养因子(brain derived neurotrophic factor,BD N F)联合应用对兔脊髓外伤性截瘫结构和功能修复的影响,以寻求建立一种更有效的治疗脊髓外伤性截瘫的疗法。方法:采用改良A llen打击法建立兔脊髓外伤性截瘫的模型,模型建立成功后观察3d再分别行注射生理盐水(对照组)、BM SCs、BD N F和BM SCs+BD N F干预。脊髓损伤移植后1、3、5周进行改良Tarlov评分及CSEP评测,5周观察脊髓结构的变化。结果:与BM SCs组、BD N F组相比,移植后第5周,BM SCs+BD N F联合移植组脊髓损伤处结构恢复较好,第3周、5周后,BM SCs+BD N F组的CSEP和改良Tarlov评分亦明显好于BM SCs组和BD N F组(P<0.01)。结论:BM SCs和BD N F联合移植对急性脊髓外伤性截瘫的结构和功能有更好的修复作用。展开更多
基金Qinghai Provincial Agricultural Science and Technology Achievements Transformation and Extension Project(2013-N-531).
文摘Background:The aim of this work is to detect and compare the peripheral blood mi RNA expression profiles in patients with severe traumatic brain injury(s TBI)2,12,24,48,and 72 h after injury at high altitude and to predict the target genes of differential expressed mi RNAs.Methods:Twenty s TBI patients from high-altitude areas were randomly selected according to the inclusion and exclusion criteria and were divided into five groups:the 2-h group,12-h group,24-h group,48-h group,and 72-h group.Peripheral blood mi RNA expression profiles were detected using real-time quantitative PCR(q RT-PCR).Results:The expression levels of mi R-18 a,mi R-203,mi R-146 a,mi R-149,mi R-23 b,and mi R-let-7 b in peripheral blood showed significant differences between the 2-h group and the 12-h group.The expression levels of mi R-203,mi R-146 a,mi R-149,mi R-23 b,and mi R-let-7 f in peripheral blood were up-regulated in the 24-h group.In the 48-h group,the expression levels of mi R-181 d,mi R-29 a,and mi R-18 b were upregulated.In the 72-h group,the expression levels of mi R-203,mi R-146 a,mi R-149,mi R-23 b,and mi R-let-7 f changed.The main target genes of the differentiation expressed mi RNAs were genes that regulate inflammatory responses,apoptosis,and DNA damage/repair.Conclusions:mi RNAs may be involved in the pathogenesis of s TBI by dynamically regulating the target genes that regulate inflammatory responses,apoptosis,and DNA damage/repair pathways.
文摘62 patients with traumatic paraplegia were treated with acupuncture(GovernorVessel electro-stimulation),according to the theories of TCM such as dredging the meridians,regu-lating vital energy and blood,improving blood circulation,reinforcing marrow and replenishing brain.The effect of acupuncture on urinary bladder disturbance was studied.The results indicated that totaleffect rate was 96.8%(Ⅰ—Ⅲ),marked effect rate was 66.1%(Ⅰ—Ⅱ).The marked effect rate incomplete paraplegia was significantly different from that in imcomplete paraplegia;it was 63.0% and87.5% respectively.Furthermore,the results suggested that the degree of spinal cord injury was veryimprotant factor that affected the effect of acupuncture therapy.
基金supported by grants from the National Natural Science Foundation of China, Nos. 81930031 (to JNZ), 81720108015 (to JNZ), 81901525 (to SZ), 82101440 (to DDS), 81801234 (to YZ) and 82071389 (to GLY)the Natural Science Foundation of Tianjin, Nos. 20JCQNJC01270 (to JWW), 20JCQNJC00460 (to GLY), 18JCQNJC81000 (to HTR)+4 种基金Scientific Research Project of Tianjin Education Commission (Natural Science), No. 2018KJ052 (to ZWZ)Tianjin Health and Health Committee Science and Technology Project, No. QN20015 (to JWW)the Science & Technology Development Fund of Tianjin Education Commission for Higher Education, No. 2016YD02 (to YW)Tianjin Key Science and Technology Projects of Innovative Drugs and Medical Devices, No. 19ZXYXSY00070 (to YW)the Clinical Research Fundation of Tianjin Medical University, No. 2018kylc002 (to YW)
文摘Neuroinflammation and the NACHT,LRR,and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury(TBI).Maraviroc,a C-C chemokine receptor type 5 antagonist,has been viewed as a new therapeutic strategy for many neuroinflammatory diseases.We studied the effect of maraviroc on TBI-induced neuroinflammation.A moderate-TBI mouse model was subjected to a controlled cortical impact device.Maraviroc or vehicle was injected intraperitoneally 1 hour after TBI and then once per day for 3 consecutive days.Western blot,immunohistochemistry,and TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling)analyses were performed to evaluate the molecular mechanisms of maraviroc at 3 days post-TBI.Our results suggest that maraviroc administration reduced NACHT,LRR,and PYD domains-containing protein 3 inflammasome activation,modulated microglial polarization from M1 to M2,decreased neutrophil and macrophage infiltration,and inhibited the release of inflammatory factors after TBI.Moreover,maraviroc treatment decreased the activation of neurotoxic reactive astrocytes,which,in turn,exacerbated neuronal cell death.Additionally,we confirmed the neuroprotective effect of maraviroc using the modified neurological severity score,rotarod test,Morris water maze test,and lesion volume measurements.In summary,our findings indicate that maraviroc might be a desirable pharmacotherapeutic strategy for TBI,and C-C chemokine receptor type 5 might be a promising pharmacotherapeutic target to improve recovery after TBI.
文摘Traumatic axonal injury is a progressive process evoked by shear forces on the brain, gradually evolving from focal axonal alteration and cumulating in neural disconnection. Clinical classifiers and conventional neuroimaging are limited in traumatic axonal injury detection, outcome prediction, and treatment guidance. Diffusion weighted imaging is an advanced magnetic resonance imaging (MRI) technique that is sensitive to the movement of water molecules, providing additional information on the micro-structural arrangement of tissue. Quantitative analysis of diffusion metrics can aid in the localization of axonal injury and/or de(dys)myelination caused by trauma. Diffusion MRI tractography is an extension of diffusion weighted imaging, and can provide additional information about white matter pathways and the integrity of brain neural networks. Both techniques are able to detect the early micro-structural changes caused by Traumatic Brain Injury (TBI), and can be used to increase understanding of the mechanisms of brain plasticity in recovery after brain injury and possibly optimize treatment planning of patients with Traumatic Brain Injury. This review focuses on the theoretical basis and applied advanced techniques of diffusion weighted imaging, their limitations and applications, and future directions in the application to TBI.
基金Supported,in part,by Grants from the National Basic Research Program of China(No.2005CB522602)National Natural Science Foundation of China(No.30672178,30872683,30800437)National Natural Science Outstanding Youth Foundation of China(No.30125020)
文摘Sepsis and subsequent multiple organ dysfunction syndrome(MODS) are frequent complications after severe traumata or burns involving a large area,and these remain as the two most common causes of morbidity and mortality in critical illnesses.Despite the recent rapid advances in intensive
文摘目的:观察骨髓间质干细胞(bone m arrow strom a cells,BM SCs)和脑源性神经营养因子(brain derived neurotrophic factor,BD N F)联合应用对兔脊髓外伤性截瘫结构和功能修复的影响,以寻求建立一种更有效的治疗脊髓外伤性截瘫的疗法。方法:采用改良A llen打击法建立兔脊髓外伤性截瘫的模型,模型建立成功后观察3d再分别行注射生理盐水(对照组)、BM SCs、BD N F和BM SCs+BD N F干预。脊髓损伤移植后1、3、5周进行改良Tarlov评分及CSEP评测,5周观察脊髓结构的变化。结果:与BM SCs组、BD N F组相比,移植后第5周,BM SCs+BD N F联合移植组脊髓损伤处结构恢复较好,第3周、5周后,BM SCs+BD N F组的CSEP和改良Tarlov评分亦明显好于BM SCs组和BD N F组(P<0.01)。结论:BM SCs和BD N F联合移植对急性脊髓外伤性截瘫的结构和功能有更好的修复作用。
