A new rapid method for the analysis of chromate ion in water by using high performance capillary electrophoresis is described. The detection limit is 1.0 mu g/ml by using 100 m mol/l Tris-HCl buffer containing 2 m mol...A new rapid method for the analysis of chromate ion in water by using high performance capillary electrophoresis is described. The detection limit is 1.0 mu g/ml by using 100 m mol/l Tris-HCl buffer containing 2 m mol/l 1,6-hexanediamine which was used as an electroosmosis modifier to improve the separation and detection sensitivity.展开更多
The paper deals with the development of a rapid and efficient Capillary Zone Electrophoresis (CZE) method for Quality Control analysis of pharmaceutical preparations containing antihistamines, decongestants, anticholi...The paper deals with the development of a rapid and efficient Capillary Zone Electrophoresis (CZE) method for Quality Control analysis of pharmaceutical preparations containing antihistamines, decongestants, anticholinergic remedies and preservatives. Active ingredients of interest are: ChlorPheniramine Maleate (CPM), DiPhenhydramine Hydrochloride (DPH), Ephedrine hydrochloride (E), Isopropamide Iodide (II), Pheniramine Maleate (PM), Lidocaine hydrochloride (L), Tetracaine hydrochloride (T), Clopamide Hydrochloride (CH), DiHydroErgochristine (DHE), PhenylEphrine hydrochloride (PE) and Acetaminophen (A). Preservatives studied are: MethylParaben (MeP), EthylParaben (EtP), PropylParaben (PrP), ButylParaben (BuP), p-HydroxyBenzoic Acid (p-HBA). All these analytes were separated in a single run using 60 mM tetraborate buffer solution (TBS) pH = 9.2 as a BackGround Electrolyte (BGE) by using an uncoated fused silica capillary of I.D. = 50 mm and applying a voltage of 25 kV in the first part of the electropheretic run (up to 5.8 min) and 30 kV for the remaining time. The hydrodynamic pressurization of the inlet vial was 20 psi at 7.2 min. up to the end of analysis. Total separation time was of 7.5 min. The method was then successfully validated and applied to the simultaneous determination of active ingredients and preservatives. Good repeatability, linearity, and sensitivity were demonstrated. Precision of migration time (tm) was RSD% < 0.53% and of corrected peak area (Ac) was RSD% < 6.15%. The linearity evaluation gave 0.9928 2 1.000. LOD and LOQ, accuracy (recovery) and ruggedness were evaluated for each analyte demonstrating the good reliability of the method. Analyses of some pharmaceutical real samples were performed.展开更多
Development of effective chromatographic or electrophoretic separation involves judicious deciding of selection of optimal experimental conditions that can provide an adequate resolution at a reasonable run time for t...Development of effective chromatographic or electrophoretic separation involves judicious deciding of selection of optimal experimental conditions that can provide an adequate resolution at a reasonable run time for the separation of interested components. Box-Behnken factorial design was effectively applied for the separation optimization of eight structurally related sulfonamides using capillary zone electrophorosis and reverse high performance liquid chromatography. Optimum values for volume ratio of THF to H2O in eluent, column temperature and flow rate of eluent are found as 12 to 88, 35℃ and 1.0 mL/min, respectively. Box-Behnken modified optimization model is extended to separation by capillary electrophoresis (CE). While using CE, a satisfactory separation is achieved with a minimum resolution larger than 1.0 for a separation time less than 10 min.展开更多
A rapid and sensitive CZE (Capillary Zone Electrophoresis) method for pharmaceutical analysis was developed and fully validated. The active compounds: Pseudoephedrine hydrochloride (PSE), Triprolidine hydrochloride (T...A rapid and sensitive CZE (Capillary Zone Electrophoresis) method for pharmaceutical analysis was developed and fully validated. The active compounds: Pseudoephedrine hydrochloride (PSE), Triprolidine hydrochloride (TRI) and Paracetamol (PAR) were separated and quantitatively determined using the tris-borate 30 mM buffer at pH = 9.0 as a Background Electrolyte (BGE). The electrophoretic separation was carried out at 25 kV in an unmodified fused silica capillary of I.D. = 50 μm with a “bubble-cell” for UV detection at 210 nm and 25°C. The separation was reached in about 3 min. After calibration the method was applied for analysis of three commercially available pharmaceutical preparations. The repeatability (RSD%) of migration time (tm) was ranging between 0.47% and 0.90% and of peak areas (A) between 0.63% and 3.64%. The Limit of Detection (LOD) values was of 0.19 μg/mL, 0.31 μg/mL and 0.08 μg/mL for respectively PSE, TRI and PAR. The results obtained in this study showed that the proposed method was useful in routinely analysis of pharmaceuticals.展开更多
Separation and determination of metal ions based on the formation of chelate anions with 1-Nitroso-2, 7-dihydrexynaphthalene-3, 6-disulfonic acid (1N2, 7O3, 6S) was studied by rising HPCE of the nine metal ions examin...Separation and determination of metal ions based on the formation of chelate anions with 1-Nitroso-2, 7-dihydrexynaphthalene-3, 6-disulfonic acid (1N2, 7O3, 6S) was studied by rising HPCE of the nine metal ions examined, the ions that can be detected sensitively with 1-Nitroso-2, 7-dihydrexynaphthalene-3, 6-disulfonic acid were Fe2+, Co2+, Cu2+, Ni2+, Zn2+ and Pd2+. The cobalt chelate could exist in two oxidation states of cobalt. When TBA(+) were added in electrophoretic solutions, the drastic changes in elertrophoretic mobilities of chelate were observed, which was due to the ion association between chelates anions and TBA(+). The ion association constants of chelate anions with TBA(+) were determined by using the change in electrophoretic mobilities of chelates, metal ions tested were separated within 10 min using 30cm silica capillary (50 m i. d).展开更多
In order to reveal the relationship between migration time and solute structure aswell as electrophoretic operation conditions in high performance capillary zoneelectrophoresis (HPCZE), researchers have presented many...In order to reveal the relationship between migration time and solute structure aswell as electrophoretic operation conditions in high performance capillary zoneelectrophoresis (HPCZE), researchers have presented many empirical and semi-empiricalmodels. These models have two main dissatisfactory points. Firstly, a constant is im-展开更多
The samples of licorice (Glycyrrhiza uralensis Fisch.) from 14 different cultivated areas were determined by the method of high Performance Capillary Electrophoresis (HPCE) for the contents of glycyrrihizic acid (GA) ...The samples of licorice (Glycyrrhiza uralensis Fisch.) from 14 different cultivated areas were determined by the method of high Performance Capillary Electrophoresis (HPCE) for the contents of glycyrrihizic acid (GA) in root. The results showed that the licorice plants come from various cultivated areas of China has different contents of GA. The GA content of licorice from Zhaodong in Heilongjiang Province is the highest, followed by those from E抰uoke, Chifeng, and Hangjin Banner in Inner Mongolia. Some suggestions for establishing the production base of licorice were put forward based on the study.展开更多
文摘A new rapid method for the analysis of chromate ion in water by using high performance capillary electrophoresis is described. The detection limit is 1.0 mu g/ml by using 100 m mol/l Tris-HCl buffer containing 2 m mol/l 1,6-hexanediamine which was used as an electroosmosis modifier to improve the separation and detection sensitivity.
文摘The paper deals with the development of a rapid and efficient Capillary Zone Electrophoresis (CZE) method for Quality Control analysis of pharmaceutical preparations containing antihistamines, decongestants, anticholinergic remedies and preservatives. Active ingredients of interest are: ChlorPheniramine Maleate (CPM), DiPhenhydramine Hydrochloride (DPH), Ephedrine hydrochloride (E), Isopropamide Iodide (II), Pheniramine Maleate (PM), Lidocaine hydrochloride (L), Tetracaine hydrochloride (T), Clopamide Hydrochloride (CH), DiHydroErgochristine (DHE), PhenylEphrine hydrochloride (PE) and Acetaminophen (A). Preservatives studied are: MethylParaben (MeP), EthylParaben (EtP), PropylParaben (PrP), ButylParaben (BuP), p-HydroxyBenzoic Acid (p-HBA). All these analytes were separated in a single run using 60 mM tetraborate buffer solution (TBS) pH = 9.2 as a BackGround Electrolyte (BGE) by using an uncoated fused silica capillary of I.D. = 50 mm and applying a voltage of 25 kV in the first part of the electropheretic run (up to 5.8 min) and 30 kV for the remaining time. The hydrodynamic pressurization of the inlet vial was 20 psi at 7.2 min. up to the end of analysis. Total separation time was of 7.5 min. The method was then successfully validated and applied to the simultaneous determination of active ingredients and preservatives. Good repeatability, linearity, and sensitivity were demonstrated. Precision of migration time (tm) was RSD% < 0.53% and of corrected peak area (Ac) was RSD% < 6.15%. The linearity evaluation gave 0.9928 2 1.000. LOD and LOQ, accuracy (recovery) and ruggedness were evaluated for each analyte demonstrating the good reliability of the method. Analyses of some pharmaceutical real samples were performed.
基金Project(20235010) support by the NSFC-KOSEF Scientific Cooperation ProgramProject supported by the Program for New Century Talents of University in Henan ProvinceProgram for Backbone Teacher in Henan Province, China
文摘Development of effective chromatographic or electrophoretic separation involves judicious deciding of selection of optimal experimental conditions that can provide an adequate resolution at a reasonable run time for the separation of interested components. Box-Behnken factorial design was effectively applied for the separation optimization of eight structurally related sulfonamides using capillary zone electrophorosis and reverse high performance liquid chromatography. Optimum values for volume ratio of THF to H2O in eluent, column temperature and flow rate of eluent are found as 12 to 88, 35℃ and 1.0 mL/min, respectively. Box-Behnken modified optimization model is extended to separation by capillary electrophoresis (CE). While using CE, a satisfactory separation is achieved with a minimum resolution larger than 1.0 for a separation time less than 10 min.
文摘A rapid and sensitive CZE (Capillary Zone Electrophoresis) method for pharmaceutical analysis was developed and fully validated. The active compounds: Pseudoephedrine hydrochloride (PSE), Triprolidine hydrochloride (TRI) and Paracetamol (PAR) were separated and quantitatively determined using the tris-borate 30 mM buffer at pH = 9.0 as a Background Electrolyte (BGE). The electrophoretic separation was carried out at 25 kV in an unmodified fused silica capillary of I.D. = 50 μm with a “bubble-cell” for UV detection at 210 nm and 25°C. The separation was reached in about 3 min. After calibration the method was applied for analysis of three commercially available pharmaceutical preparations. The repeatability (RSD%) of migration time (tm) was ranging between 0.47% and 0.90% and of peak areas (A) between 0.63% and 3.64%. The Limit of Detection (LOD) values was of 0.19 μg/mL, 0.31 μg/mL and 0.08 μg/mL for respectively PSE, TRI and PAR. The results obtained in this study showed that the proposed method was useful in routinely analysis of pharmaceuticals.
文摘Separation and determination of metal ions based on the formation of chelate anions with 1-Nitroso-2, 7-dihydrexynaphthalene-3, 6-disulfonic acid (1N2, 7O3, 6S) was studied by rising HPCE of the nine metal ions examined, the ions that can be detected sensitively with 1-Nitroso-2, 7-dihydrexynaphthalene-3, 6-disulfonic acid were Fe2+, Co2+, Cu2+, Ni2+, Zn2+ and Pd2+. The cobalt chelate could exist in two oxidation states of cobalt. When TBA(+) were added in electrophoretic solutions, the drastic changes in elertrophoretic mobilities of chelate were observed, which was due to the ion association between chelates anions and TBA(+). The ion association constants of chelate anions with TBA(+) were determined by using the change in electrophoretic mobilities of chelates, metal ions tested were separated within 10 min using 30cm silica capillary (50 m i. d).
基金Project supported by the National Natural Science Foundation of China.
文摘In order to reveal the relationship between migration time and solute structure aswell as electrophoretic operation conditions in high performance capillary zoneelectrophoresis (HPCZE), researchers have presented many empirical and semi-empiricalmodels. These models have two main dissatisfactory points. Firstly, a constant is im-
文摘The samples of licorice (Glycyrrhiza uralensis Fisch.) from 14 different cultivated areas were determined by the method of high Performance Capillary Electrophoresis (HPCE) for the contents of glycyrrihizic acid (GA) in root. The results showed that the licorice plants come from various cultivated areas of China has different contents of GA. The GA content of licorice from Zhaodong in Heilongjiang Province is the highest, followed by those from E抰uoke, Chifeng, and Hangjin Banner in Inner Mongolia. Some suggestions for establishing the production base of licorice were put forward based on the study.
