Chinese herbal compound is playing an important role on curing human diseases.And it has been a trend that Chinese herbal compound is being used all over the world in 21 century.However,our Chinese herbal compound is ...Chinese herbal compound is playing an important role on curing human diseases.And it has been a trend that Chinese herbal compound is being used all over the world in 21 century.However,our Chinese herbal compound is facing serious challenge for the lack of canonical system of quality criterion for Chinese herbal compound so it has been a urgent problem to set up the quality control standards and reveal therapeutic basis of Chinese herbal compound.In order to give full play to the advantages of Chinese herbal compound,modern scientific and technological is used to research of Chinese herbal compound,especially the high performance liquid chromatography tandem mass spectrometry(HPLC-MS),because it is high sensitive,rapid,and obtain more information.It is very necessary that HPLC-MS is uesed to elucidate the effective components of basic substances of Chinese Herbal Compound,and endow traditional Chinese medicine with modern scientific connotation.展开更多
建立了化妆品中包括乙基己基三嗪酮在内22种防晒剂的高效液相色谱-串联质谱(high-performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)确证方法。选择具有代表性的霜类和乳类基质的防晒化妆品作为研究对象。样品...建立了化妆品中包括乙基己基三嗪酮在内22种防晒剂的高效液相色谱-串联质谱(high-performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)确证方法。选择具有代表性的霜类和乳类基质的防晒化妆品作为研究对象。样品经过超声提取、过滤后,采用C_(18)色谱柱分离、电喷雾正离子源(ESI+)电离、多反应监测(MRM)模式扫描测定,确定了22种防晒剂的质谱确证离子对及质谱参数、流动相及梯度洗脱方法,得到的图谱峰型好,响应高。加标确证试验得出22种防晒剂的相对离子丰度比均在最大允许偏差范围内,相对离子丰度比的相对标准偏差(RSD)为0.4%~9.3%(n=10),出峰时间的RSD为0~0.5%(n=10)。该方法灵敏度高,准确性好,可用于化妆品中22种防晒剂的质谱确证分析,为实验室检测提供了技术支持,有助于化妆品防晒剂的质量监督。展开更多
A high performance liquid chromatography coupled with electrospray ionization-tandem mass spectrome try（HPLC-ESI-MS/MS） method was developed for the analysis and identification of ginsenosides in the extracts of raw...A high performance liquid chromatography coupled with electrospray ionization-tandem mass spectrome try（HPLC-ESI-MS/MS） method was developed for the analysis and identification of ginsenosides in the extracts of raw Panax ginseng（RPG） and steamed Panax ginseng at high temperatures（SPGHT）. A total of 25 ginsenosides were extracted include of which 10 low-polar ginsenosides, such as ginsenosides F4, Rk3, Rh4, 20S-Rg3, 20R-Rg3 and so on, were identified according to their HPLC retention time and MS/MS data. The results indicated that the low polar ginsenosides were seldom found in RPG. For the exploration of the transformation pattern of the ginsenosides in steam processing, the standards of ginsenosides Re, Rg1, Rb1, Rc, Rb2, Rb3 and Rd were selected and hydrolyzed at a temperature of 120 oC. The results show that these polar ginsenosides can be converted to low-polar ginsenosides such as Rg2, Rg6, F4, Rk3 and Rg5 by hydrolyzing the sugar chains.展开更多
文摘Chinese herbal compound is playing an important role on curing human diseases.And it has been a trend that Chinese herbal compound is being used all over the world in 21 century.However,our Chinese herbal compound is facing serious challenge for the lack of canonical system of quality criterion for Chinese herbal compound so it has been a urgent problem to set up the quality control standards and reveal therapeutic basis of Chinese herbal compound.In order to give full play to the advantages of Chinese herbal compound,modern scientific and technological is used to research of Chinese herbal compound,especially the high performance liquid chromatography tandem mass spectrometry(HPLC-MS),because it is high sensitive,rapid,and obtain more information.It is very necessary that HPLC-MS is uesed to elucidate the effective components of basic substances of Chinese Herbal Compound,and endow traditional Chinese medicine with modern scientific connotation.
文摘建立了化妆品中包括乙基己基三嗪酮在内22种防晒剂的高效液相色谱-串联质谱(high-performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)确证方法。选择具有代表性的霜类和乳类基质的防晒化妆品作为研究对象。样品经过超声提取、过滤后,采用C_(18)色谱柱分离、电喷雾正离子源(ESI+)电离、多反应监测(MRM)模式扫描测定,确定了22种防晒剂的质谱确证离子对及质谱参数、流动相及梯度洗脱方法,得到的图谱峰型好,响应高。加标确证试验得出22种防晒剂的相对离子丰度比均在最大允许偏差范围内,相对离子丰度比的相对标准偏差(RSD)为0.4%~9.3%(n=10),出峰时间的RSD为0~0.5%(n=10)。该方法灵敏度高,准确性好,可用于化妆品中22种防晒剂的质谱确证分析,为实验室检测提供了技术支持,有助于化妆品防晒剂的质量监督。
基金Supported by the Key Project of Jilin Provincial Science and Technology Department, China(No.20090908)the Project of Changchun Science and Technology Bureau, China(No.2008256)the Special Cooperative Project for Hitech Industrializa-tion of Jilin Provincial Science and Chinese Academy of Sciences, China(No.2009SYHZ0026)
文摘A high performance liquid chromatography coupled with electrospray ionization-tandem mass spectrome try（HPLC-ESI-MS/MS） method was developed for the analysis and identification of ginsenosides in the extracts of raw Panax ginseng（RPG） and steamed Panax ginseng at high temperatures（SPGHT）. A total of 25 ginsenosides were extracted include of which 10 low-polar ginsenosides, such as ginsenosides F4, Rk3, Rh4, 20S-Rg3, 20R-Rg3 and so on, were identified according to their HPLC retention time and MS/MS data. The results indicated that the low polar ginsenosides were seldom found in RPG. For the exploration of the transformation pattern of the ginsenosides in steam processing, the standards of ginsenosides Re, Rg1, Rb1, Rc, Rb2, Rb3 and Rd were selected and hydrolyzed at a temperature of 120 oC. The results show that these polar ginsenosides can be converted to low-polar ginsenosides such as Rg2, Rg6, F4, Rk3 and Rg5 by hydrolyzing the sugar chains.
