Energy dissipation of cavity expansion based on generalized non-linear failure criterion under high stresses

Based on the compression mechanism for analyzing the cavity expansion problem in soil under high stresses,generalized non-linear failure criterion and large strain and energy conservation in plastic region during the cavity expanding were adopted.The energy conservation equation was established and the limited pressure of cavity expansion under high stresses was given based on the energy dissipation analysis method,in which the energy generated from cavity expansion is absorbed by the volume change and shear strain caused in soil.The factors of large strain and dilatation were considered by the proposed method.The analysis shows that the limited pressure is determined by failure criterion,stress state,large deformation characteristic,dilatation and strength of soil.It is shown from the comparison that the results with the proposed method approximate to those of the in-situ method.The cavity expansion pressure first decreases and then increases nonlinearly with both of shear modulus and dilatation increasing.

Disturbance failure mechanism of highly stressed rock in deep excavation:Current status and prospects

This article reviews the current status on the dynamic behavior of highly stressed rocks under disturbances.Firstly,the experimental apparatus,methods,and theories related to the disturbance dynamics of deep,high-stress rock are reviewed,followed by the introduction of scholars’research on deep rock deformation and failure from an energy perspective.Subsequently,with a backdrop of highstress phenomena in deep hard rock,such as rock bursts and core disking,we delve into the current state of research on rock microstructure analysis and residual stresses from the perspective of studying the energy storage mechanisms in rocks.Thereafter,the current state of research on the mechanical response and the energy dissipation of highly stressed rock formations is briefly retrospected.Finally,the insufficient aspects in the current research on the disturbance and failure mechanisms in deep,highly stressed rock formations are summarized,and prospects for future research are provided.This work provides new avenues for the research on the mechanical response and damage-fracture mechanisms of rocks under high-stress conditions.

Support design method for deep soft-rock tunnels in non-hydrostatic high in-situ stress field

Due to the long-term plate tectonic movements in southwestern China,the in-situ stress field in deep formations is complex.When passing through deep soft-rock mass under non-hydrostatic high in-situ stress field,tunnels will suffer serious asymmetric deformation.There is no available support design method for tunnels under such a situation in existing studies to clarify the support time and support stiffness.This study first analyzed the mechanical behavior of tunnels in non-hydrostatic in-situ stress field and derived the theoretical equations of the ground squeezing curve(GSC)and ground loosening curve(GLC).Then,based on the convergence confinement theory,the support design method of deep soft-rock tunnels under non-hydrostatic high in-situ stress field was established considering both squeezing and loosening pressures.In addition,this method can provide the clear support time and support stiffness of the second layer of initial support.The proposed design method was applied to the Wanhe tunnel of the China-Laos railway in China.Monitoring data indicated that the optimal support scheme had a good effect on controlling the tunnel deformation in non-hydrostatic high in-situ stress field.Field applications showed that the secondary lining could be constructed properly.

Resistance index and browning mechanism of apple peel under high temperature stress

Apples are one of the most important economic crops worldwide.Because of global warming and an aggravation of environmental,abnormally high temperatures occur frequently in fruit-growing season and seriously affect normal fruit growth and reduce fruit quality and yield.We took five-year-old Ruixue’(Qinfu 1×Pink Lady;CNA20151469.1) fruits as test materials,and the ambient temperature during fruit development was monitored.The results showed that during the fruit-growing season,especially during the rapid growth stage (July to August),the maximum daily temperature exceeded 30℃ and lasted for more than 40 days.To determine the effects of high temperature stress on the apple fruit resistance,we treated expanding,veraison,and maturity-period fruits at different temperatures.It was found that the fruits of the expanding period showed strong resistance to high temperature stress,whereas during veraison and maturity,fruit resistance to high temperature stress decreased,and the fruit peel browning phenotype appeared.Meanwhile,the content of malonaldehyde (MDA),hydrogen peroxide (H_(2)O_(2)),and superoxide anion (O._(2)^(-)) in the peel gradually increased with increasing temperature.The content of total phenols,flavanol,and flavonoids in the peel decreased substantially at 45℃.Moreover,it was found that polyphenol oxidase gene (MdPPO1) was most sensitive to high temperature stress in apple.Furthermore,transient and stable MdPPO1 overexpression significantly promoted peel browning.The transgenic materials were more sensitive to high temperatures,and browning was more severe compared to non-genetically modified organism (WT).Stable MdPPO1 knockout calli obtained via clustered regularly interspersed short palindromic repeats (CRISPR/Cas9) gene knockout technology reduced the browning phenotype,and the resultant fruits were not sensitive to the effects of high temperature stress.Thus,MdPPO1 expression may be a key factor of high temperature-related changes observed in the browning phenotype that provides a scientific theoretical basis for the selection of high temperature-resistant varieties and apple cultivation and management in the future.

Potassium alleviated high nitrogen-induced apple growth inhibition by regulating photosynthetic nitrogen allocation and enhancing nitrogen utilization capacity

There is a close relationship between potassium(K)and nitrogen(N).However,the roles of K under high N conditions remain unclear.Using a hydroponics approach,we monitored the morphological,physiological,and molecular changes in M9T337 apple(Malus domestica)rootstocks under different nitrate(10 and 30 mmol·L^(-1)NO_(3)^(-))and K supply(0.5,6,10,and 20 mmol·L_(-1)K^(+))conditions.Results revealed that high nitrate inhibited the root growth of M9T337 rootstocks,downregulated the expressions of K transporter genes(MdPT5,MdHKT1,and MdATK1),and reduced the net NO3-and K+influx at the surface of roots,thereby resulting in an N/K imbalance in rootstocks.Further investigation showed that 10 mmol·L^(-1)K increased the activity of N metabolic enzymes(NR,GS,NiR,and GOGAT),upregulated the expressions of genes related to nitrate uptake and transport(MdNRT1.1,MdNRT1.2,MdNRT1.5,and MdNRT2.4),promoted15N transport from the roots to the shoots,optimized leaf N distribution,and improved photosynthetic N utilization efficiency under high nitrate conditions.These results suggest that the negative effects of high nitrate may be related to the N/K imbalance and that reducing N/K in plants by increasing K supply level can effectively alleviate the inhibition of N assimilation by high nitrate stress.

Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage

Due to climate change, extreme heat stress events have become more frequent, adversely affecting rice yield and grain quality. The accumulation and translocation of dry matter and nitrogen substances are essential for rice yield and grain quality. To assess the impact of high temperature stress(HTS) at the early panicle initiation(EPI) stage on the accumulation, transportation, and distribution of dry matter and nitrogen substances in various organs of rice, as well as the resulting effects on rice yield and grain quality, pot experiments were conducted using an indica rice cultivar Yangdao 6(YD6) and a japonica rice cultivar Jinxiangyu 1(JXY1) under both normal temperature(32 ℃/26 ℃) and high temperature(38 ℃/29 ℃) conditions. The results indicated that exposure to HTS at the EPI stage significantly decreased rice yield by reducing spikelet number per panicle, grain-filling rate, and grain weight. However, it improved the nutritional quality of rice grains by increasing protein and amylose contents. The reduction in nitrogen and dry matter accumulation accounted for the changes in spikelet number per panicle, grain-filling rate, and grain size. Under HTS, the decrease in nitrogen accumulation accompanied by the reduction in dry matter may be due to the down-regulation of leaf net photosynthesis and senescence, as evidenced by the decrease in nitrogen content. Furthermore, the decrease in sink size limited the translocation of dry matter and nitrogen substances to grains, which was closely related to the reduction in grain weight and the deterioration of grain quality. These findings significantly contribute to our understanding of the mechanisms of HTS on grain yield and quality formation from the perspective of dry matter and nitrogen accumulation and translocation. Further efforts are needed to improve the adaptability of rice varieties to climate change in the near future.

Triaxial creep damage characteristics of sandstone under high crustal stress and its constitutive model for engineering application

The creep characteristics of rock under high crustal stress are of important influence on the long‐term stability of deep rock engineering.To study the creep characteristics and engineering application of sandstone under high crustal stress,this study constructed nonlinear creep damage(NCD)constitutive mode based on the triaxial graded loading‒unloading creep test of sandstone in the Yuezhishan Tunnel.A numerical NCD constitutive model and a breakable lining(BL)model were developed based on FLAC3D and then applied to the stability analysis of the Yuezhishan Tunnel.Based on the creep test results of sandstone,a power function of creep rate and stress level was constructed,by which the long‐term strength was solved.The results show that the long‐term strength of the red sandstone based on the related function of the steady‐state creep rate and stress level is close to the measured stress value in engineering.The NCD model considering damage factors reflects the instantaneous and viscoelastic plasticity deformation characteristics of the red sandstone.The numerical NCD constitutive model and the BL model can reflect surrounding rock deformation characteristics and lining failure characteristics in practical engineering.The research results provide theoretical references for long‐term stability analysis of rock engineering and the deformation control of surrounding rock under high crustal stress.

Metabolomics of astaxanthin hyperaccumulation in Haematococcus pluvialis under high light stress

Variation in metabolite profiles of Haematococcus pluvialis(a type of unicellular green algal)under light stress is a key issue of study at the present.To investigate the effect of light intensity on accumulation of astaxanthin in H.pluvialis,a 26-day batch culture experiment of H.pluvialis under the light intensity levels at 73,127,182,236,and 291μmol/(m^(2)·s)was conducted.Therefore,the optimal light intensity and the corresponding metabolic pathways of accumulation in H.pluvialis were determined.Results show that 236μmol/(m^(2)·s)was the optimum light intensity to induce astaxanthin accumulation,at which a maximum content of 9.01 mg/L was achieved on Day 24.A total of 132 metabolites were identified and quantified,of which 38 differential metabolites were highlighted and classified,including 3 fatty acids or intermediates,5 amino acids or derivatives,5 carbohydrates or intermediates,16nucleoside derivatives,and 9 other metabolites using LC-MS/MS technique.Subsequently,16 statistically significant differential metabolic pathways were enriched and annotated based on Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis between the control and the 236μmol/(m^(2)·s)treatment group(P<0.05).In addition,the bioprocesses included cellular basal metabolism and signaling systems,such as carbohydrate metabolism,amino acid metabolism,glycerol and derivatives metabolism,nucleotide and derivative metabolism,and inositol phosphate metabolism were activated and regulated under strong light stress conditions.Moreover,4 hub metabolites containing D-glucose-6-phosphate,L-tyrosine,glycerol-3-phosphate,and L-glutamine were identified,based on which the associated metabolic network was constructed.The study provided a metabolomic view of astaxanthin accumulation in H.pluvialis under strong light stress.

Study on Asymmetric Deformation Law and Surrounding Rock Control Technology of High Stress Soft Rock Crossing Roadway

In order to solve the problem of asymmetric large deformation of high-stress soft rock crossing roadway under complex geological conditions in deep mines, taking the 2# total return airway of 76.2# section of Wuyang Coal Mine as the engineering background, the causes of asymmetric deformation and failure of soft rock crossing roadway in deep mines were summarized and analyzed by means of field investigation, theoretical analysis and numerical simulation, and the asymmetric high-efficiency support technology with large row spacing was studied. The results show that the lithology of roadway strata is the main cause of asymmetric deformation and failure of roadway. The shape change of roadway is not the main influencing factor of asymmetric deformation of roadway, but for the control of roadway surrounding rock, the straight wall semi-circular arch roadway is better than the rectangular roadway. The field industrial test shows that after adopting the new support design scheme, the displacement of the roof and floor of the roadway is reduced by 86.39% compared with the original support design scheme, and the displacement of the two sides of the roadway is reduced by 86.05% compared with the original support design scheme, which can ensure the normal and safe production of the roadway during the service period, and provide reference for the support design of other similar geological conditions.

Fatigue reliability quantitative analysis of cement concrete for highway pavement under high stress ratio

In order to obtain the change law of the fatigue reliability of cement concrete for highway pavement under high stress ratios, first, the probability densities of monotonic random variables including concrete fatigue life are deduced. And then, the fatigue damage probability densities of the Miner and Chaboche-Zhao models are deduced. By virtue of laboratory fatigue test results, the fatigue damage probability density functions of the two models can be obtained, considering different stress ratios. Finally, substituting load cycles into them, the change law of cement concrete fatigue reliability about load cycles can be acquired. The results show that under the same stress ratio, with the increase in the load cycle, the fatigue reliability declines from almost 100% to 0% gradually. No matter under what stress ratio, during the initial stage of the load action, there is always a relatively stable phase for fatigue reliability. With the increase in the stress ratio, the stable phase gradually shortens and the load cycle corresponding to the reliability of 0% also decreases. In the descent phase of reliability, the higher the stress ratio is, the lower the concrete reliability is for the same load cycle. Besides, compared with the Chaboche-Zhao fatigue damage model, the Miner fatigue damage model is safer.

Effects of High Light Stress on Chlorophyll-protein Complexes of Two Subspecies of Rice

Influence of high light stress on the photosynthesis of flag leaves of indica subspecies (cv. “Shanyou 63', sensitive to photoinhibition) and japonica subspecies (cv. “Wuyujing', resistant to photoinhibition) of rice ( Oryza sativa L.) was comparatively investigated. In both cultivars of rice, the excitation energy distribution between two photosystems was altered and the excitation energy transfer from light harvesting chlorophyll protein complexes to PSⅡ was inhibited by high light stress. These decreases were more pronounced in indica rice cultivar as compared to japonica one. The analysis of mild SDS_PAGE showed that in indica rice, high light stress almost disaggregated the trimer of light harvesting chlorophyll protein complexes of PSⅡ (LHC Ⅱ 1). The stress reduced the contents of internal antennae chlorophyll protein complexes of PSⅡ (CPa), light harvesting chlorophyll protein of PSⅠ (CPⅠa) and Chl a protein complex of PSⅠ reaction center (CPⅠ) as well as dimer of LHCⅡ (LHCⅡ 2) in indica rice. In japonica subspecies, however, high light stress depressed the contents of LHCⅡ 1, CPa and CPⅠa, but slightly impacted on CPⅠ content. Moreover, the increase in the contents of monomer of LHCⅡ by high light stress was found in both subspecies. In consistent with above results, analysis of polypeptide indicated that the amounts of 27 kD and 25 kD polypeptide of LHCⅡ in particular, as well as that of 21 kD polypeptide of CPⅠa were reduced by high light stress in both subspecies. It was found that, comparing with japonica rice, the stress pronouncedly diminished 43 kD and 47 kD proteins of CPa and 23 kD extrisic protein in indica rice.

Numerical simulation of optimum mining design for high stress hard-rock deposit based on inducing fracturing mechanism

The 3D numerical simulation model of deep hard-rock deposit in Kaiyang Phosphate Mine of Guiyang was established based on the practical engineering using 3DEC numerical simulation software. The distribution characteristics of displacement fields and plastic zones of the orebody were simulated in three different excavation cases, including the case of excavation artificial inducted roadway in the orebody, the case of horizontal or vertical excavation direction and the case of the upward or downward excavation order. The simulation results indicate that the plastic zone and displacement field of surrounding rock around the inducted roadway are continuously increasing with the increase of the exposure time after digging an artificial inducted roadway in the orebody. Thus the raw rock ore becomes easier to be fragmented, which provides advantageous conditions for roadheader to cut high stress hard-rock. It is worthy noting that there is a large difference in effective utilization of deep ground pressure between horizontal and vertical excavation directions. The later can produce larger deformation and fracture zone than the former on the rock mass around the deduced roadway, which means that the later may utilize the high ground pressure more effectively to break hard-rock. And the obtained results also show that upward excavation order is more helpful for ground pressure to break rock than downward excavation order.

Effects of Short-term High Temperature Stress on the Photosynthesis of Potato in Different Growth Stages

[Objective] The aim was to study the effects of short-term high temperature stress on the photosynthesis of potato in different growth stages. [Method] Choosing powder potato named Longshu No.3 widely cultivated in Ningxia as test material,the changes of stomata conductance （Gs）,transpiration rate （Tr） and CO2 concentration difference between internal and external leaf chamber,net photosynthetic rate （Pn） and photosynthetic water use efficiency （WUE） in different growth stages under short-term high temperature were analyzed. [Result] During seedling stage,the hysteretic nature of net photosynthetic rate and CO2 concentration difference between internal and external leaf chamber of potato could be found under high temperature stress,while the change trends of stomata conductance and transpiration rate under high temperature stress were consistent to that at normal temperature,but stomata conductance and transpiration rate were higher than those at normal temperature,and CO2 concentration difference between internal and external leaf chamber affected net photosynthetic rate most obviously. During branching stage,the change trends of net photosynthetic rate,CO2 concentration difference between internal and external leaf chamber,stomata conductance and transpiration rate under high temperature stress and normal temperature were similar,but they changed abruptly and reached peak value at noon under high temperature stress,while there existed consistent variation of water use efficiency under high temperature stress and at normal temperature,and CO2 concentration difference between internal and external leaf chamber also affected net photosynthetic rate most greatly,next came transpiration rate. [Conclusion] High temperature stress affected the photosynthesis of potato in different growth stages,and it was more obvious during branching stage than seedling stage,while CO2 concentration difference between internal and external leaf chamber had the most important influence on net photosynthetic rate.

Effects of High Temperature Stress on Growth of Stress-Tolerant Rice Seedlings with Resistibility

Two heat-tolerant rice varieties, N5 and TQ, were chosen as test materi- als. Specifically, rice seedlings （leaf age at 2.1）, cultivated in room, were treated at 40 ℃ for 7 d and some indices were measured, including plant height, dry weight, leaf color, proline, malondialdehyde and conductivity. The results showed that high temperature advanced the growth of N5 seedlings, for example, plant height, root length and dry weight of ground parts all increased. However, high temperature prevented TQ seedlings growth, plant height in particular. Furthermore, high temper- ature treatment increased the content of chlorophyll of N5 and had none effects on PSII of N5, with little damages on membrane system. On the other hand, high temperature actually reduced PSII activity of TQ, and seriously damaged TQ mem- brane system. It is speculated that the differences of the two varieties lie on pro- duction or removing capacity of reactive oxide species.

Effect of High-Gate-Voltage Stress on the Reverse Gated-Diode Current in LDD nMOSFET’s

The reverse generation current under high-gate-voltage stress condition in LDD nMOSFET＇s is studied. We find that the generation current peak decreases as the stress time increases. We ascribe this finding to the dominating oxide trapped electrons that reduce the effective drain bias, lowering the maximal generation rate. The density of the effective trapped electrons affecting the effective drain bias is calculated with our model.

Assessment of High Temperature Tolerance of Two Dactylis glomerata Materials

The high temperature tolerance of two Dactylis glomerata materials, D. glom-erata cv. Baoxing and D. glomerata cv. Jinsiling, was evaluated under artificial con-ditions. The relative conductance rate, free proline content and SOD activity in seedling leaves was detected. Results showed that with the increase of temperature, the relative conductance rate and free proline content increased in both two materi-als, while the activity of SOD increased first and then decreased, indicating that the two materials had heat-resistant mechanism. D. glomerata cv. Baoxing presented slightly higher resistance against high temperature under experimental conditions compared with D. glomerata cv. Jinsiling.

Rate transient analysis methods for water-producing gas wells in tight reservoirs with mobile water

Tight gas reservoirs with mobile water exhibit multi-phase flow and high stress sensitivity.Accurately analyzing the reservoir and well parameters using conventional single-phase rate transient analysis methods proves challenging.This study introduces novel rate transient analysis methods incorporating evaluation processes based on the conventional flowing material balance method and the Blasingame type-curve method to examine fractured gas wells producing water.By positing a gas-water two-phase equivalent homogenous phase that considers characteristics of mobile water,gas,and high stress sensitivity,the conventional single-phase rate transient analysis methods can be applied by integrating the phase's characteristics and defining the phase's normalized parameters and material balance pseudotime.The rate transient analysis methods based on the equivalent homogenous phase can be used to quantitatively assess the parameters of wells and gas reservoirs,such as original gas-in-place,fracture half-length,reservoir permeability,and well drainage radius.This facilitates the analysis of production dynamics of fractured wells and well-controlled areas,subsequently aiding in locating residual gas and guiding the configuration of well patterns.The specific evaluation processes are detailed.Additionally,a numerical simulation mechanism model was constructed to verify the reliability of the developed methods.The methods introduced have been successfully implemented in field water-producing gas wells within tight gas reservoirs containing mobile water.

Morphology and Genetics of Rice in Response to High Temperature at Flowering Period

High temperature stress is one of major abiotic stresses limiting rice productivity,especially at the flowering period.Understanding mechanisms of rice adaptation to heat stress would facilitate the development of heat-tolerance cultivars for improving yield in a warmer world.Rice heat stress responses are very complex.Interactions between structure,function and the environment need to be investigated at the apparent and molecular levels in order to obtain a full picture.In this review,we summarized the current knowledge on the morphology and genetic basis of heat tolerance in reproductive tissues of rice at the flowering time,and some morphologic characters for increasing thermotolerance in rice via conventional breeding are outlined.

Failure mechanism of bolting support and high-strength bolt-grouting technology for deep and soft surrounding rock with high stress

In deep underground mining, the surrounding rocks are very soft with high stress. Their deformation and destruction are serious, and frequent failures occur on the bolt support. The failure mechanism of bolt support is proposed to solve these problems. A calculation theory is established on the bond strength of the interface between the anchoring agent and surrounding rocks. An analysis is made on the influence law of different mechanical parameters of surrounding rocks on the interfacial bond strength. Based on the research, a new high-strength bolt-grouting technology is developed and applied on site. Besides, some helpful engineering suggestions and measures are proposed. The research shows that the serious deformation and failure, and the lower bond strength are the major factors causing frequent failures of bolt support. So, the bolt could not give full play to its supporting potential. It is also shown that as the integrity, strength, interface dilatancy and stress of surrounding rocks are improved, the bond strength will increase. So, the anchoring force on surrounding rocks can be effectively improved by employing an anchoring agent with high sand content, mechanical anchoring means, or grouting reinforcement. The new technology has advantages in a high strength, imposing pre-tightening force, and giving full play to the bolt supporting potential. Hence, it can improve the control effect on surrounding rocks. All these could be helpful references for the design of bolt support in deep underground mines.

Failure characteristics of high stress rock induced by impact disturbance under confining pressure unloading

The failure characteristics under coupled static and dynamic loading were investigated by the improved split Hopkinson pressure bar （SHPB） with axial pre-pressure and confining pressure. The results show that the stress—strain curve of the rock under static-dynamic coupled loading is a typical class I curve when the dynamic load is comparatively high; With the decrease of the dynamic load, the stress—strain curve transforms to a typical class II curve. The dynamic failure process was recorded by high-speed photography. Analyses of fracture surface morphology show that the failure modes of specimens are tensile failure or combined shear failure when the impact load energy is low, but the failure modes of specimens become tensile failure when the impact load energy is high. The results of fractal dimension show that the elastic potential energy release leads to increase in the degree of crushing of samples when the energy of impact load is low under coupled static and dynamic loads with high stress.