Brassinosteroids Mediate Endogenous Phytohormone Metabolism to Alleviate High Temperature Injury at Panicle Initiation Stage in Rice

High temperatures cause physiological and biochemical changes and significantly affect young panicle development of rice(Oryza sativa L.).Brassinosteroids play important roles in enhancing crop stress resistance.In this study,we subjected rice cultivars Huanghuazhan(heat-resistant)and IR36(heat-sensitive)to high temperature(HT,40 oC)or normal temperature(NT,33 oC)for 7 d at the panicle initiation stage,in conjunction with application of 24-epibrassinolide[EBR,a synthetic brassinolide(BR)]or brassinazole(BRZ,a BR biosynthesis inhibitor)at the beginning of the treatments.HT exacerbated spikelet degeneration and inhibited young panicle growth,which were partially prevented by EBR application,while BRZ application aggravated the reduction in spikelet number.HT decreased the contents of BR,active cytokinins(aCTK),active gibberellins(aGA)and indole-3-acetic acid(IAA),but increased the content of abscisic acid(ABA)in young panicles.The activities of key enzymes involved in sucrose hydrolysis,glycolysis and the tricarboxylic acid cycle in young panicles were decreased with the change of endogenous hormone levels under HT.In addition,the contents of H2O2 and malondialdehyde(MDA)were increased and the activities of antioxidant enzymes were decreased in young panicles.Exogenous application of EBR induced the expression of phytohormone biosynthesis-related genes and down-regulated the expression of phytohormone catabolism-related genes to increase the contents of endogenous BR,aCTK,aGA and ABA,thus promoting the decomposition and utilization of sucrose in young panicles,enhancing the activities of superoxide dismutase,catalase and peroxidase,and reducing the accumulation of H2O2 and MDA in young panicles,whereas application of BRZ had the opposite physiological effects.These results showed that brassinosteroids mediate endogenous phytohormone metabolism to alleviate HT injury at the panicle initiation stage in rice.

Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage

Due to climate change, extreme heat stress events have become more frequent, adversely affecting rice yield and grain quality. The accumulation and translocation of dry matter and nitrogen substances are essential for rice yield and grain quality. To assess the impact of high temperature stress(HTS) at the early panicle initiation(EPI) stage on the accumulation, transportation, and distribution of dry matter and nitrogen substances in various organs of rice, as well as the resulting effects on rice yield and grain quality, pot experiments were conducted using an indica rice cultivar Yangdao 6(YD6) and a japonica rice cultivar Jinxiangyu 1(JXY1) under both normal temperature(32 ℃/26 ℃) and high temperature(38 ℃/29 ℃) conditions. The results indicated that exposure to HTS at the EPI stage significantly decreased rice yield by reducing spikelet number per panicle, grain-filling rate, and grain weight. However, it improved the nutritional quality of rice grains by increasing protein and amylose contents. The reduction in nitrogen and dry matter accumulation accounted for the changes in spikelet number per panicle, grain-filling rate, and grain size. Under HTS, the decrease in nitrogen accumulation accompanied by the reduction in dry matter may be due to the down-regulation of leaf net photosynthesis and senescence, as evidenced by the decrease in nitrogen content. Furthermore, the decrease in sink size limited the translocation of dry matter and nitrogen substances to grains, which was closely related to the reduction in grain weight and the deterioration of grain quality. These findings significantly contribute to our understanding of the mechanisms of HTS on grain yield and quality formation from the perspective of dry matter and nitrogen accumulation and translocation. Further efforts are needed to improve the adaptability of rice varieties to climate change in the near future.

Adjuvant Pelvic Radiotherapy vs.Sequential Chemoradiotherapy for High-Risk StageⅠ-ⅡEndometrial Carcinoma

Objective To explore if the addition of adjuvant chemotherapy with paclitaxel and carboplatin to radiotherapy confers an advantage for overall survival(OAS),and progression free survival(PFS);to assess the incidence of relapses over standard pelvic radiotherapy; and to evaluate the related toxicity in high-risk stageⅠ-Ⅱendometrial carcinoma Methods Medical records were reviewed to identify high-risk stageⅠ-Ⅱendometrial carcinoma cases treated in the Clinical Oncology and Nuclear Medicine department between 2002 and 2008 with adjuvant radiotherapy alone(armⅠ)(57 patients) or with sequential carboplatin(AUC5-6) and paclitaxel(135-175 mg/m^2) with radiotherapy(armⅡ)(51 patients).Radiotherapy was performed through the four-field box technique at doses of 45-50 Gy(1.8 Gy/day×5 days/week). Results The toxicity was manageable and predominantly hematologic with a grade 3 neutropenia and thrombocytopenia in 9.8% and 6% of the patients in armⅠand armⅡ,respectively,without febrile neutropenia.All patients experienced hair loss. Chemoradiotherapy arm was associated with a lower incidence rate of relapse(9.8% vs.22.7% ).After a median follow-up period of 48 months,the 5-year OAS and PFS rates for chemoradiotherapy-treated patients were significantly more favorable than those who did not receive chemotherapy(P=0.02 and 0.03,respectively).In armⅠ,the OAS and PFS rates were 73.7% and 66.7% compared with those in armⅡ,whose rates were 90.2% and 84.3% . Conclusions Adjuvant chemoradiation with paclitaxel and carboplatin improved the survival rates and decreased the recurrence rates in patients with high-risk stageⅠ-Ⅱendometrial carcinoma.Chemotherapy was associated with an acceptable rate of toxicity. However,a prospective study with a larger number of patients is needed to define a standard adjuvant treatment for high-risk stageⅠ-Ⅱendometrial carcinoma.

A Method for Calculating Oil Field Relative Permeability Curve by Using Water Drive Characteristic Curve in High Water Cut Stage

With the production of strong bottom water reservoir, it will soon enter the ultra-high water cut stage. After entering the ultra-high water cut period, the main means of stable production is liquid extraction. Large liquid volume has a certain impact on the physical property distribution and fluid seepage law of the oilfield. The relative permeability curve measured according to the industry standard is not used for the prediction of development indicators and the understanding of the dynamic law of the oilfield. In order to understand the characteristics of water drive law in high water cut stage of water drive oilfield, starting from the water drive characteristic curve in high water cut stage, the method for calculating the relative permeability curve is deduced. Through numerical simulation verification and fitting the actual production data, it is confirmed that the obtained relative permeability curve is in line with the reality of the oilfield, It can provide some guiding significance for understanding the production law and water drive law of strong bottom water reservoir in ultra-high water cut stage.

How to win the “qualitative change” stage The 13th Annual China Textile Roundtable focused on “high-quality development”

On January 21,the 13th Annual China Textile Roundtable,"Shuyang Cup"·The 6th China Textile News Award Ceremony,and the First Award Ceremony of the Qualified Enterprises for the Review of the Quality Awards of the National Textile Industry were held in Beijing.

HkV和HRCT检查在职业性尘肺病诊断中的价值

目的探究高千伏胸片(HkV)和高分辨率计算机体层X线摄影术(HRCT)检查在职业性尘肺病诊断中的临床应用价值。方法回顾性分析133例进行职业病诊断的尘肺病患者资料,患者均接受HkV和HRCT检查,以临床病理检查为金标准,比较HkV和HRCT对尘肺患者肺部阴影的显示情况、图片质量、分期诊断符合率及尘肺合并症的检出情况。结果HRCT对肺部P影、小阴影检出率高于HkV(P<0.05),两组大阴影检出率比较,差异无统计学意义(P>0.05);HRCT检查图像质量有效率高于HkV检查(P<0.05);HRCT诊断总准确率高于HkV诊断(P<0.05);HRCT对肺部感染、肺气肿、胸膜增厚、气胸的检出率高于HkV(P<0.05),HRCT与HkV对肺结核、肺大泡检出率差异无统计学意义(P>0.05)。结论HRCT检查对职业性尘肺病患者肺部阴影、临床分期及合并症的诊断效果高于HkV,图片质量更高。

玉米花粉高温胁迫相关miRNA的筛选及其靶基因分析

以高耐热玉米品种郑单958、低耐热玉米品种先玉335为材料,以正常生长条件为对照,在花期进行高温胁迫,通过miRNA高通量测序筛选玉米花粉中的差异表达miRNA,然后预测其靶基因,并对靶基因的本体特征和代谢通路进行富集分析。结果表明,共筛选到818个miRNA前体序列。在郑单958高温胁迫花粉与对照花粉对比组(HT958 vs CK958)中共筛选到19个显著差异表达miRNA序列,其中15个miRNA序列上调表达,4个下调表达,3个miRNA序列达到极显著水平(P<0.01)。对这19个显著差异表达miRNA的靶基因进行预测,共获得了503个基因转录本,其富集较多的GO生物学过程条目分别为转录调控DNA-模板、微管生物学过程、磷酸化作用、RNA聚合酶Ⅱ正向调控转录过程、甲基化作用等,KEGG富集较显著的代谢通路分别是谷胱甘肽代谢、碳代谢、花生四烯酸代谢、糖酵解/糖异生、叶酸生物合成等。在先玉335高温胁迫花粉与对照花粉对比组(HT335 vs CK335)中共筛选到15个显著差异表达miRNA序列,其中7个miRNA序列上调表达,8个下调表达,1个miRNA序列达到了极显著水平(P<0.01)。对这15个显著差异表达miRNA的靶基因进行预测,共获得了454个基因转录本,其富集较多的GO生物学过程条目分别为转录调控DNA-模板、磷酸化作用、蛋白质磷酸化作用、蛋白质水解、DNA修复等,富集较显著的KEGG代谢通路分别是其他多糖降解、亚油酸代谢、代谢通路、硫胺素代谢、内质网内蛋白质加工过程等。在郑单958高温胁迫花粉与先玉335高温胁迫花粉对比组(HT985 vs HT335)中共筛选到85个显著差异表达miRNA序列,其中35个miRNA序列为上调表达,50个为下调表达,24个miRNA序列达到了极显著水平(P<0.01)。对这85个显著差异表达miRNA的靶基因进行预测,共获得了2 286个基因转录本,其富集较多的GO生物学过程条目分别为转录调控DNA-模板、磷酸化作用、蛋白质磷酸化、蛋白质水解、跨膜转运等,富集较显著的代谢通路分别是鞘脂类代谢、淀粉和蔗糖代谢、其他多糖降解、代谢通路、半胱氨酸及甲硫氨酸代谢等。在HT958 vs CK958与HT335 vs CK335对比组中共筛选到94个显著差异表达miRNA序列,其中(预测全新)PC-3p-10069_1143、(预测全新)PC-3p-18335_646、(玉米)zma-miR164f-5p等28个miRNA序列达到了极显著水平(P<0.01)。对这94个显著差异表达miRNA的靶基因进行预测,共获得了4 569个基因转录本,其富集较多的GO生物学过程条目分别为转录调控DNA-模板、磷酸化作用、蛋白质磷酸化、蛋白质转运、蛋白质水解等,其富集较显著的KEGG代谢通路分别是内质网内蛋白质加工过程、真核生物核糖体生物合成、剪接体、鞘脂类代谢、内吞作用等。

高速铁路动静态轨检数据里程对齐与误差修正

轨道几何动、静检测数据间的精确匹配对探明高速铁路线路服役状态和制定准确可靠的养护维修策略具有关键作用。针对动静里程匹配算法研究较少的现状,提出利用动、静态实测数据波形匹配,建立基于互相关函数与动态时间规划相结合的两阶段修正算法,并以距离误差作为评价指标。结合某高铁线路轨道几何检测数据的案例分析,以静态检测数据作为参考基准,对动态检测数据进行里程误差评估与修正。结果表明,两阶段算法修正效果显著,累积距离误差降幅超过93%,修正后的动、静态检测数据严格对齐保证了动态检测数据的可靠性与有效性。

绿色视角下的高铁站房腔体植入策略与模拟验证研究

由于体量和形式的特殊性,高铁站房的舒适度问题比一般建筑更为突出,以绿色视角审视其设计具有较大的优化潜力。近年来建筑学界对腔体的关注增多,而高铁站房方案设计中针对性地设置腔体、操作方法等方面的研究尚显不足。立足于方案设计阶段,针对空间特征与现存问题,从宏观层面上构建了对候车环境品质多重导控的腔体层级,探讨了“结构腔”、“功能腔”和“绿景腔”三类腔体的植入方式。构建典型模型并生成研究方案用于量化研究。通过性能模拟技术,验证了腔体植入对室内气流、采光质量有显著提升的效果。腔体植入策略打破了当前高铁站房固定的空间模式,为我国高铁站房的绿色创新提供参考,在实践中具有广泛的应用价值。

高质量发展下区域绿色物流效率评价研究

区域绿色物流是推进物流业绿色发展的重要元素。基于DFSO提出高质量发展视域下绿色物流框架并构建效率测度指标体系,运用改进三阶段DEA模型对中国30个省(市)进行实证研究,分析各因素对绿色物流效率的影响。结果表明高质量发展视域下省域绿色物流整体效率不高,地区差异明显,纯技术效率是制约其发展的主要因素;高质量发展各方面均对绿色物流效率有不同程度的影响,但二者间的协调仍处初级阶段。基于此,提出高质量发展视域下区域绿色物流效率提升的对策建议。研究能够为相关部门衡量高质量发展时期物流业绿色发展成效、精准施策提供参考。

数字赋能农村公共服务高质量:发展视域、内在逻辑与实践路径

数字赋能农村公共服务高质量发展,是中国式现代化的应有之义和重要途径。作为一个发展视域性命题,数字赋能农村公共服务,超越微观服务质量管理逻辑。数字技术突破时空、资源限制,赋能农村公共服务,让农业农村农民提升人力资本、享有数字红利与共享公共服务资源,促进微观农民个体的全面发展、中观农业农村现代化与宏观城乡融合发展。公共服务数字化是数字赋能农村公共服务高质量的重要机制,然而现实中存在数字人才、数字基础设施、数字资源配置等三大限度。超越农村公共服务数字化现实限度,要从农民数字素养培养、农村数字基础设施投资、城乡数字公共资源体制改革等方面着力,以更好发挥数字赋能农村公共服务高质量发展的价值作用。

三阶段自适应采样和增量克里金辅助的昂贵高维优化算法

代理辅助进化算法已广泛应用于求解代价高昂的多目标优化问题,但大多数由于代理模型的局限性而仅限于解决决策变量低维的问题。为了解决高维的昂贵多目标优化问题,提出了一种基于三阶段自适应采样策略的改进增量克里金辅助的进化算法。该算法使用改进的增量克里金模型来近似每个目标函数,此模型的超参数根据预测的不确定性进行自适应更新,降低计算复杂度的同时保证模型在高维上的准确性;此外,在模型管理方面提出一种三阶段自适应采样的策略,将采样过程分为不同的优化阶段以更有针对性的选择个体,能够首先保证收敛性,提高算法的收敛速度。为了验证算法的有效性,在包含各种特征的两组测试问题DTLZ(deb-thiele-laumanns-zitzler)、MaF(many-objective function)和路径规划实际工程问题上与最新的同类型算法进行实验对比,结果表明该算法在解决决策变量高维的昂贵多目标优化问题上具有较强的竞争力。

MHR和SII早期联合检测对急性一氧化碳中毒患者病情及预后的评估

目的分析单核细胞与高密度脂蛋白胆固醇比值(MHR)、系统性免疫炎症指数(SII)在急性一氧化碳中毒(ACOP)患者中变化,探讨其早期联合检测对ACOP患者的病情及预后的评估。方法选取2019年1月—2023年1月在河北医科大学哈励逊国际和平医院急救医学部收治的ACOP患者120例,根据患者病情严重程度将其分为轻度中毒组30例、中度中毒组35例和重度中毒组55例;根据患者是否发生急性一氧化碳中毒迟发性脑病(DEACMP)情况分为DEACMP组17例和预后良好组103例;同期健康体检者30例作为对照组。所有患者均在入院时,对照组于体检时,检测MHR、SII、白细胞介素-18(IL-18)和C反应蛋白(CRP)的变化,记录患者60 d内DEACMP发生情况。通过Logistic回归分析ACOP发生DEACMP的独立危险因素。绘制受试者工作特征(ROC)曲线,计算曲线下面积(AUC),评估MHR和SII对ACOP患者发生DEACMP的预测价值。结果入院时,不同中毒程度患者MHR、SII、IL-18和CRP水平均高于对照组,中度高于轻度,重度高于中度、轻度,均有统计学差异(P<0.05)。3组患者DEACMP发生率分别为0,5.71%和27.27%,有统计学差异(χ^(2)=14.777,P=0.001)。相关分析结果显示,ACOP患者中毒程度与DEACMP发生率呈正相关(r=0.648,P=0.000)。Logistic回归分析结果显示,MHR、SII水平升高均是ACOP患者发生DEACMP的危险因素(OR=7.757、5.169,均P<0.01)。入院时MHR、SII、IL-18、CRP及MHR+SII预测发生DEACMP的AUC分别为0.840、0.862、0.771、0.706和0.899,MHR和SII联合应用预测能力优于MHR、SII、IL-18和CRP(P<0.05)。结论MHR和SII是ACOP患者发生DEACMP的独立危险因素,MHR和SII早期联合检测有助于ACOP患者病情和预后的评估。

极端耗水层带形成机制及流场调控增效模式——以陆相砂岩特高含水后期整装油田为例

以胜利油区陆相砂岩油藏整装油田为代表,主力单元进入特高含水后期(含水率大于95%),局部区域出现极端耗水现象,水油比急剧上升,注入水利用率大幅下降,吨油操作成本成倍增加,经济效益变差,但油藏中还有60%左右剩余地质储量。注入水沿着极端耗水层带窜流是制约陆相砂岩整装油田特高含水后期效益开发的关键问题。以提高特高含水老油田开发效益为目标,明晰了极端耗水层带形成机制及调控机理,建立了基于老井的变流线调控极端耗水层带扩波及方法,形成特高含水后期油藏精准描述及调控极端耗水层带扩波及的效益开发技术体系。通过应用流场调控技术,使传统认为含水率98%近废弃油藏开展示范应用,基于极端耗水层带流场调控经济寿命期延长10a以上,产油量大幅增加,含水率下降,吨油操作成本下降,实现了特高含水后期老油田低成本开发。

海上高含水油井分段开采的选井方法应用研究

海上边底水油藏一旦见水会迅速进入高含水阶段,而分段开采可以达到抑制高含水和延长油井寿命的目的。因此,可利用分段开采机理模型对含水上升影响因素进行敏感性分析,研究高含水油井分段开采的选井方法依据,并利用数值模拟方法进行验证。结果表明:1)底水油藏水平井含水上升影响因素敏感性大小排序为黏度>高低渗级差>高渗条带比例>最大产液>高渗段渗透率。2)根据生产动态、水平段地质资料及邻井资料将海上底水油藏高含水水平井见水模式分为线性见水全面水淹、点状见水局部水淹和多点见水全面水淹,其中点状见水局部水淹模式可进行分段开采。以典型井A1为例,利用所建选井方法流程判断含水率曲线为线状见水特征,开井含水高,水平段渗透率分段特征明显,Ⅲ-1小层水淹程度较高,A1井跟部水淹严重,且由于第1套夹层遮挡,A1井趾部水淹程度较低,为点状见水模式局部水淹,符合分段开采特征。3)针对点状见水局部水淹水平井,采用分段开采进行稳油控水,利用数值模拟方法模拟预测,分段开采方案开发较不分段方案产油量和采收率得到显著提升,验证了选井方法及分段开采的适用性。提出的选井方法流程可为现场水平井分段开采选井研究提供方法依据。

穗分化期高温对水稻生长发育的影响及缓解措施研究进展

保障水稻产量是应对全球人口增长以及粮食危机的关键举措之一。水稻穗分化期遭遇高温天气的概率日益增加,并对该区域内水稻的安全生产造成了极大威胁,因此开展相关研究意义重大。文章从幼穗形成、花药结构和花粉发育、产量与品质以及对水稻生理指标、代谢调控机理的影响等方面综述了穗分化期高温对水稻的影响并提出相应的缓解措施,并对研究前景作出展望,以期为开展水稻逆境生理研究以及缓解措施的合理选择提供依据。

灌浆期高温胁迫对早籼稻品质性状的影响

【目的】研究灌浆期高温胁迫对早籼稻碾磨、外观和蒸煮食味品质的影响,揭示早籼稻品质性状对灌浆期高温胁迫的响应规律。【方法】以R996、湘早籼45号和6个新育成的早籼稻品系为试验材料,以分期播种方式利用自然高温进行胁迫处理,分析了供试品系的碾磨、外观和蒸煮食味品质,筛选出对高温钝感和敏感的水稻品系,探究稻米品质性状对灌浆期高温胁迫的响应规律。【结果】稻米品质性状对灌浆期不同时段温度的敏感性不同,始穗后10 d内温度对稻米碾磨品质和蒸煮食味品质影响最显著,始穗后30 d内温度对稻米外观品质影响最显著。稻米品质对灌浆期高温胁迫的响应因性状而异,灌浆期高温胁迫显著降低糙米率、精米率、整精米率和粒长,显著提高垩白粒率、垩白度,劣化了稻米碾磨品质和外观品质;显著降低直链淀粉含量,提高米饭外观、米饭口感和米饭黏度,提升了米饭适口性。水稻品系对高温胁迫的响应因稻米品质性状而异,Q3、Q8、Q11、Q13属于碾磨品质高温钝感品系,Q3、Q4、Q5和Q11属于外观品质高温钝感品系且高温下蒸煮食味品质优。【结论】灌浆期高温胁迫会降低稻米的碾磨品质和外观品质,提高米饭适口性。水稻品系Q3和Q11碾磨品质、外观品质好且高温钝感,蒸煮食味品质优良,可望为早籼稻优质育种提供新的种质资源。

四川盆地仪陇—平昌地区侏罗系凉高山组页岩油地质特征及富集条件

基于钻井岩心的地球化学及其他实验分析结果,结合生产测试资料,对四川盆地仪陇—平昌地区侏罗系凉高山组页岩油地质特征及富集条件进行研究,并划分了勘探有利区。研究结果表明:(1)仪陇—平昌地区凉高山组为早侏罗世第3次湖盆扩张期的沉积产物,整体经历了凉下段滨湖—浅湖、凉上段中—下部深湖—半深湖及浅湖、凉上段上部三角洲前缘和浅湖的沉积环境变化。(2)研究区凉高山组页岩可分为长英质页岩、钙质页岩、黏土质页岩等3种;页岩有机质丰度高,TOC值为0.50%~3.39%,平均值为1.30%,有机质类型以Ⅱ_(1)和Ⅱ_(2)型为主,热演化程度高,镜质体反射率(Ro)为1.00%~1.90%,处于高—过成熟阶段。(3)凉高山组页岩物性较好,孔隙度为0.48%~7.17%,平均为3.61%,发育无机成因孔、有机孔和构造缝等储集空间类型;含油性较好,以轻质组分为主,整体呈零散状、点状分布,局部呈团块状富集;凉上段下部富有机质页岩更发育,是目前勘探的重点层位。(4)凉高山组页岩油气的富集受控于前陆盆地过渡期的弱沉降环境、大面积分布的深湖相沉积、较高的热演化程度以及普遍发育的微裂缝。中南部为页岩油气潜力区,页岩储层厚度一般大于15 m,TOC值大于1.50%,孔隙度大于4.00%,R_(o)大于1.30%;凉高山组页岩油资源量为26.75×10^(8)t、页岩气资源量为1.72×10^(12)m^(3),有望成为四川盆地石油产量增加的主力层系。

一种具有超宽输入功率范围的三路整流电路

本文提出一种具有超宽输入功率范围的三路整流电路.通过在三条整流支路中采用不同阈值电压的二极管,使每条支路对不同输入功率进行高效整流,三条整流支路协同作用,使所提出的整流电路能够在超宽输入功率范围内保持高效率工作;设计并实现了一个工作在2.4 GHz的三支路整流电路,三条整流支路分别实现在-10~11、11~24和24~35 dBm的功率范围内,效率保持在30%以上.测试结果表明:在-6~25 dBm的输入功率范围内,电路的转换效率均可保持在40%以上,最高效率可以达到71.2%.所提出的整流电路对输入功率的灵敏度较低,能够在超宽输入功率范围内实现高效率能量转换,可用于能量收集或动态无线能量传输系统中.