Two hundred and thirty-six patinets with various advanced malignant solid tumors treated by combined chemotherapy with routine doses of cisplatin (DDP) from 1980 to 1986 are presented. According to different doses of ...Two hundred and thirty-six patinets with various advanced malignant solid tumors treated by combined chemotherapy with routine doses of cisplatin (DDP) from 1980 to 1986 are presented. According to different doses of cisplatin everyday, the patients were divided into 4 groups: (1) 20 ing/day×4- 5, 80 cases; (2) 30 mg day × 3 - 5, 91 cases; (3) 40 mg/ day 3 -4, 37 cases; (4) 50 mg/day×2 - 3, 28 cases. Each group was repeated for 3 weeks. The effect and toxicity were analysed and compared with 22 cases treated by single DDP in 1975. The response (CR+PR) rate was 39.2% in 194 evaluated patients. The response rate was similar in group 20 mg and single DDP (29.2% and 27.3%). Ths response rate was lower than that of group 30 mg, 40 mg, and 50 mg 43.4% and 50%) (P<0.05). The remissions in various groups were not significantly different.The toxicity of combined chemotherapy was not severe. 91.1% of patients had nausea and vomiting. There was no statistical difference in the various groups. Bone marrow suppresion was less in single DDP group than that of combined chemotherapy group (P<0.05), DDP 30-50 mg 1/d×5-3 was better than HD-DDP in some patients.展开更多
背景白藜芦醇不仅具有抗肿瘤作用,其还能增强多种化疗药物的化疗敏感性,但其对食管癌耐药细胞的顺铂敏感性的作用尚不清楚.目的探讨白藜芦醇逆转食管癌细胞Eca109/DDP的耐药效应并从铁死亡的角度分析其潜在的作用机制.方法采用MTT法确...背景白藜芦醇不仅具有抗肿瘤作用,其还能增强多种化疗药物的化疗敏感性,但其对食管癌耐药细胞的顺铂敏感性的作用尚不清楚.目的探讨白藜芦醇逆转食管癌细胞Eca109/DDP的耐药效应并从铁死亡的角度分析其潜在的作用机制.方法采用MTT法确定白藜芦醇和顺铂的最佳作用时间和浓度;检测细胞增殖、细胞内丙二醛(malondialdehyde,MDA)、谷胱甘肽(glutathione,GSH)、活性氧(reactive oxygen species,ROS)及铁离子的水平;Western blot检测铁死亡相关调控因子酰基辅酶A合成酶长链家族成员4(acyl coenzyme A synthetase long chain family member 4,ACSL4)、铁蛋白重链(ferritin heavy chain,FTH)、谷胱甘肽过氧化物酶4(glutathione peroxidase 4,GPX4)和肿瘤蛋白53(tumor protein 53,P53)的蛋白表达情况.结果MTT检测结果显示,相较于单用顺铂,联用白藜芦醇对Eca109/DDP细胞的生长抑制作用较为显著(P<0.05).此外,白藜芦醇和顺铂联用可显著降低Eca109/DDP细胞克隆形成数(P<0.05),增加细胞内铁离子、MDA和ROS水平(P<0.05),降低GSH水平(P<0.05).铁死亡抑制剂(ferrostatin-1,Fer-1)和铁离子螯合剂(deferoxamine,DFO)可部分逆转白藜芦醇对细胞增殖的抑制作用(P<0.05).Western blot的结果显示相较于其他组,白藜芦醇和顺铂联用组的FTH和GPX4的蛋白表达显著降低(P<0.05),而ACSL4和P53的蛋白表达显著增加(P<0.05).结论白藜芦醇可通过铁死亡抑制Eca109/DDP细胞的增殖并逆转顺铂耐药.展开更多
目的:探讨叉头框转录因子M1(FoxM1)对人宫颈癌顺铂耐药株Hela/DDP增殖、侵袭、凋亡的影响及其机制。方法:将Hela/DDP细胞分为空白组(常规培养,不予任何处理)、正常对照(NC)组(转染空白干扰质粒)、低表达组和过表达组。低表达组转染siRNA...目的:探讨叉头框转录因子M1(FoxM1)对人宫颈癌顺铂耐药株Hela/DDP增殖、侵袭、凋亡的影响及其机制。方法:将Hela/DDP细胞分为空白组(常规培养,不予任何处理)、正常对照(NC)组(转染空白干扰质粒)、低表达组和过表达组。低表达组转染siRNA-FoxM1下调Hela/DDP细胞中FoxM1表达,过表达组转染pcDNA3.1-FoxM1上调Hela/DDP细胞中FoxM1表达。采用RT-qPCR法检测FoxM1 m RNA表达水平,CCK-8检测细胞活力,流式细胞仪检测细胞凋亡,Transwell小室检测细胞侵袭能力。结果:FoxM1 mRNA在Hela细胞中的表达水平低于Hela/DDP细胞(P<0.05)。空白组与NC组中FoxM1 mRNA、增殖率、凋亡率、侵袭细胞数及HSP70、S100A9蛋白表达水平比较,差异均无统计学意义(P>0.05)。与空白组比较,低表达组细胞活力和侵袭能力及FoxM1 mRNA、HSP70蛋白表达水平降低,凋亡率和S100A9蛋白表达水平升高(P<0.05),与空白组比较,过表达组细胞活力和侵袭能力及FoxM1 m RNA、HSP70蛋白表达水平升高,凋亡率和S100A9蛋白表达水平降低(P<0.05)。与低表达组比较,过表达组细胞活力和侵袭能力及FoxM1 mRNA、HSP70蛋白表达水平升高,凋亡率和S100A9蛋白表达水平降低(P<0.05)。结论:下调FoxM1表达可抑制HSP70表达,促进S100A9表达,调控宫颈癌细胞恶性行为,从而提高宫颈癌顺铂耐药细胞株对顺铂化疗的敏感性。展开更多
文摘Two hundred and thirty-six patinets with various advanced malignant solid tumors treated by combined chemotherapy with routine doses of cisplatin (DDP) from 1980 to 1986 are presented. According to different doses of cisplatin everyday, the patients were divided into 4 groups: (1) 20 ing/day×4- 5, 80 cases; (2) 30 mg day × 3 - 5, 91 cases; (3) 40 mg/ day 3 -4, 37 cases; (4) 50 mg/day×2 - 3, 28 cases. Each group was repeated for 3 weeks. The effect and toxicity were analysed and compared with 22 cases treated by single DDP in 1975. The response (CR+PR) rate was 39.2% in 194 evaluated patients. The response rate was similar in group 20 mg and single DDP (29.2% and 27.3%). Ths response rate was lower than that of group 30 mg, 40 mg, and 50 mg 43.4% and 50%) (P<0.05). The remissions in various groups were not significantly different.The toxicity of combined chemotherapy was not severe. 91.1% of patients had nausea and vomiting. There was no statistical difference in the various groups. Bone marrow suppresion was less in single DDP group than that of combined chemotherapy group (P<0.05), DDP 30-50 mg 1/d×5-3 was better than HD-DDP in some patients.
文摘背景白藜芦醇不仅具有抗肿瘤作用,其还能增强多种化疗药物的化疗敏感性,但其对食管癌耐药细胞的顺铂敏感性的作用尚不清楚.目的探讨白藜芦醇逆转食管癌细胞Eca109/DDP的耐药效应并从铁死亡的角度分析其潜在的作用机制.方法采用MTT法确定白藜芦醇和顺铂的最佳作用时间和浓度;检测细胞增殖、细胞内丙二醛(malondialdehyde,MDA)、谷胱甘肽(glutathione,GSH)、活性氧(reactive oxygen species,ROS)及铁离子的水平;Western blot检测铁死亡相关调控因子酰基辅酶A合成酶长链家族成员4(acyl coenzyme A synthetase long chain family member 4,ACSL4)、铁蛋白重链(ferritin heavy chain,FTH)、谷胱甘肽过氧化物酶4(glutathione peroxidase 4,GPX4)和肿瘤蛋白53(tumor protein 53,P53)的蛋白表达情况.结果MTT检测结果显示,相较于单用顺铂,联用白藜芦醇对Eca109/DDP细胞的生长抑制作用较为显著(P<0.05).此外,白藜芦醇和顺铂联用可显著降低Eca109/DDP细胞克隆形成数(P<0.05),增加细胞内铁离子、MDA和ROS水平(P<0.05),降低GSH水平(P<0.05).铁死亡抑制剂(ferrostatin-1,Fer-1)和铁离子螯合剂(deferoxamine,DFO)可部分逆转白藜芦醇对细胞增殖的抑制作用(P<0.05).Western blot的结果显示相较于其他组,白藜芦醇和顺铂联用组的FTH和GPX4的蛋白表达显著降低(P<0.05),而ACSL4和P53的蛋白表达显著增加(P<0.05).结论白藜芦醇可通过铁死亡抑制Eca109/DDP细胞的增殖并逆转顺铂耐药.
文摘目的:探讨叉头框转录因子M1(FoxM1)对人宫颈癌顺铂耐药株Hela/DDP增殖、侵袭、凋亡的影响及其机制。方法:将Hela/DDP细胞分为空白组(常规培养,不予任何处理)、正常对照(NC)组(转染空白干扰质粒)、低表达组和过表达组。低表达组转染siRNA-FoxM1下调Hela/DDP细胞中FoxM1表达,过表达组转染pcDNA3.1-FoxM1上调Hela/DDP细胞中FoxM1表达。采用RT-qPCR法检测FoxM1 m RNA表达水平,CCK-8检测细胞活力,流式细胞仪检测细胞凋亡,Transwell小室检测细胞侵袭能力。结果:FoxM1 mRNA在Hela细胞中的表达水平低于Hela/DDP细胞(P<0.05)。空白组与NC组中FoxM1 mRNA、增殖率、凋亡率、侵袭细胞数及HSP70、S100A9蛋白表达水平比较,差异均无统计学意义(P>0.05)。与空白组比较,低表达组细胞活力和侵袭能力及FoxM1 mRNA、HSP70蛋白表达水平降低,凋亡率和S100A9蛋白表达水平升高(P<0.05),与空白组比较,过表达组细胞活力和侵袭能力及FoxM1 m RNA、HSP70蛋白表达水平升高,凋亡率和S100A9蛋白表达水平降低(P<0.05)。与低表达组比较,过表达组细胞活力和侵袭能力及FoxM1 mRNA、HSP70蛋白表达水平升高,凋亡率和S100A9蛋白表达水平降低(P<0.05)。结论:下调FoxM1表达可抑制HSP70表达,促进S100A9表达,调控宫颈癌细胞恶性行为,从而提高宫颈癌顺铂耐药细胞株对顺铂化疗的敏感性。
