On the basis of analyzing and testing of common inverting circuits, a new type inverter, which has a special inverting circuit called complex isolated, has been put forward. In turn, an applicable air plasma cutting ...On the basis of analyzing and testing of common inverting circuits, a new type inverter, which has a special inverting circuit called complex isolated, has been put forward. In turn, an applicable air plasma cutting machine has also been put into use. Testing results show that this kind inverter has lots of properties as high reliability, high efficiency, good EMC(electromagnetic compatibility) and easy operating. The complex isolated inverting circuit can be considered as a quite valuable reference for the research of high power welding inverter.展开更多
Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 ...Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors.展开更多
在全桥倍流DC/DC拓扑的基础上加以改进,提出了一种高频隔离光伏并网逆变器拓扑,所提拓扑保留了倍流电路电流纹波小等优点;且高频变压器输出经过一级变换直接输出低频交流,无直流环节,有利于提高系统效率。引入混合型正弦脉宽脉位调制(si...在全桥倍流DC/DC拓扑的基础上加以改进,提出了一种高频隔离光伏并网逆变器拓扑,所提拓扑保留了倍流电路电流纹波小等优点;且高频变压器输出经过一级变换直接输出低频交流,无直流环节,有利于提高系统效率。引入混合型正弦脉宽脉位调制(sinusoidal pulse width and position modulation,SPWPM)策略,将正弦调制和高频逆变功能均在前级逆变器完成;后级倍流电路工作在工频和高频混合模式,有利于减小开关管损耗及保证电流平滑换流。同时为消除并网电流控制策略中静态误差等问题,采用比例谐振(proportion resonant,PR)控制器代替传统的比例积分(proportion integral,PI)控制器。仿真分析和实验结果验证了所提控制策略的可行性。展开更多
文摘On the basis of analyzing and testing of common inverting circuits, a new type inverter, which has a special inverting circuit called complex isolated, has been put forward. In turn, an applicable air plasma cutting machine has also been put into use. Testing results show that this kind inverter has lots of properties as high reliability, high efficiency, good EMC(electromagnetic compatibility) and easy operating. The complex isolated inverting circuit can be considered as a quite valuable reference for the research of high power welding inverter.
文摘Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors.
文摘在全桥倍流DC/DC拓扑的基础上加以改进,提出了一种高频隔离光伏并网逆变器拓扑,所提拓扑保留了倍流电路电流纹波小等优点;且高频变压器输出经过一级变换直接输出低频交流,无直流环节,有利于提高系统效率。引入混合型正弦脉宽脉位调制(sinusoidal pulse width and position modulation,SPWPM)策略,将正弦调制和高频逆变功能均在前级逆变器完成;后级倍流电路工作在工频和高频混合模式,有利于减小开关管损耗及保证电流平滑换流。同时为消除并网电流控制策略中静态误差等问题,采用比例谐振(proportion resonant,PR)控制器代替传统的比例积分(proportion integral,PI)控制器。仿真分析和实验结果验证了所提控制策略的可行性。
