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Estimating shear strength of high-level pillars supported with cemented backfilling using the HoekeBrown strength criterion 被引量:3
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作者 Kaizong Xia Congxin Chen +3 位作者 Xiumin Liu Yue Wang Xuanting Liu Jiahao Yuan 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第2期454-469,共16页
Deep metal mines are often mined using the high-level pillars with subsequent cementation backfilling(HLSCB)mining method.At the design stage,it is therefore important to have a reasonable method for determining the s... Deep metal mines are often mined using the high-level pillars with subsequent cementation backfilling(HLSCB)mining method.At the design stage,it is therefore important to have a reasonable method for determining the shear strength of the high-level pillars(i.e.cohesion and internal friction angle)when they are supported by cemented backfilling.In this study,a formula was derived for the upper limit of the confining pressure σ3max on a high-level pillar supported by cemented backfilling in a deep metal mine.A new method of estimating the shear strength of such pillars was then proposed based on the Hoek eBrown failure criterion.Our analysis indicates that the horizontal stress σhh acting on the cemented backfill pillar can be simplified by expressing it as a constant value.A reasonable and effective value for σ3max can then be determined.The value of s3max predicted using the proposed method is generally less than 3 MPa.Within this range,the shear strength of the high-level pillar is accurately calculated using the equivalent MohreCoulomb theory.The proposed method can effectively avoid the calculation of inaccurate shear strength values for the high-level pillars when the original HoekeBrown criterion is used in the presence of large confining pressures,i.e.the situation in which the cohesion value is too large and the friction angle is too small can effectively be avoided.The proposed method is applied to a deep metal mine in China that is being excavated using the HLSCB method.The shear strength parameters of the high-level pillars obtained using the proposed method were input in the numerical simulations.The numerical results show that the recommended level heights and sizes of the high-level pillars and rooms in the mine are rational. 展开更多
关键词 Deep metal mines high-level pillars HoekeBrown strength criterion Cemented backfilling Confining pressure Shear strength
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Research on the Path of Cultivating High-Level Technical and Skilled Talents in Vocational Undergraduate Colleges and Universities
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作者 Kan Su Seong Pek Lim Linli Yao 《Journal of Contemporary Educational Research》 2024年第7期150-157,共8页
This paper explores strategies to cultivate high-level technical talents in vocational undergraduate colleges by analyzing their development,the essence of such talents,and proposing several key measures.Strategies in... This paper explores strategies to cultivate high-level technical talents in vocational undergraduate colleges by analyzing their development,the essence of such talents,and proposing several key measures.Strategies include optimizing professional settings and curricula,enhancing school-enterprise cooperation and the integration of industry-university-research,improving teaching staff quality and educational standards,and expanding international cooperation.The importance of policy support and funding and establishing robust quality assurance systems is emphasized for effectively cultivating skilled talents in vocational undergraduate colleges. 展开更多
关键词 Vocational undergraduate colleges high-level technical talents Professional setting Innovation and entrepreneurship education
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Leave or Stay?Antecedents of High-level Talent Migration in the Pearl River Delta Megalopolis of China:from a Perspective of Regional Differentials in Housing Prices
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作者 SHEN Chen WANG Yang +1 位作者 ZUO Jian Raufdeen RAMEEZDEEN 《Chinese Geographical Science》 SCIE CSCD 2023年第6期1068-1081,共14页
Rapid urbanization and population growth have triggered an increase in urban housing demand and rising housing prices,which can influence the migration intention of high-level talents.Much work within the literature h... Rapid urbanization and population growth have triggered an increase in urban housing demand and rising housing prices,which can influence the migration intention of high-level talents.Much work within the literature has focused more on the migration of the general public.However,antecedents of migration intention and the impact of housing prices on the migration of high-level talents remain unclear.Therefore,based on the push-pull theory,this study investigates the influencing factors of talent migration intention and explores the role of housing prices.This study reveals a complex mechanism underlying migration decisions by using logistic regression models and survey data of high-level talents in the Pearl River Delta(PRD)megalopolis of China.The results indicate that:1)in high house-price regions,social integration is the primary push factor,and the main factors for retaining talents are the expectation of future work and intimate family relationships;2)in medium house-price regions,the main factors that attract talents are the current salary level and close family ties;3)in low house-price regions,living convenience is a determining factor in retaining talents.This study provides a new perspective for talent mobility research and offers valuable inputs for retaining and attracting talents in different economic development regions.Findings are of great significance for formulating talent introduction policies and forming a new pattern of rational spatial docking and effective allocation of human resources. 展开更多
关键词 destination choice migration intention high-level talents house-price pressure push-pull theory the Pearl River Delta megalopolis of China
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An Exploratory Practice of Integration of Industry and Education in High-level Programming Language Course in the Background of New Engineering
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作者 Yu Zhang Xiaohong Su +3 位作者 Jianhong Zhai Yanhang Zhang Yongfeng Yuan Yulin Zhou 《计算机教育》 2023年第12期272-279,共8页
This paper analyzes some problems of the current teaching situation in the course High-level Programming Language,such as the lagging content of the course compared with technology development,the emphasis on theory r... This paper analyzes some problems of the current teaching situation in the course High-level Programming Language,such as the lagging content of the course compared with technology development,the emphasis on theory rather than on practice,the low enthusiasm of students for learning,and the weak practical ability of students.In response to the needs of enterprises for talents under the background of New Engineering,especially the cultivation of students’adaptability and practical abilities towards future industries to improve students’knowledge and problemsolving abilities to keep up with the modern technology,this paper proposes the ways and methods to reform and explore the teaching content and teaching methods by integrating industry and education,assisting teaching according to industrial demands,and lowering technology barriers of new technology.The practical effect is evaluated through a survey in class and the follow-up questionnaire after class,and the results show that the effect of the practice is effective. 展开更多
关键词 high-level programming language New Engineering Integration of Industry and education Practical project-based courses
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Effect of drying cracks on swelling and self-healing of bentonite-sand blocks used as engineered barriers for radioactive waste disposal
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作者 Yu Tan Guangping Zhou +2 位作者 Huyuan Zhang Xiaoya Li Ping Liu 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第5期1776-1787,共12页
Experiments were conducted to evaluate the healing of drying cracks in air-dried bentonite-sand blocks after hydration and swelling in groundwater,providing justifications to simplify the protection of blocks prior to... Experiments were conducted to evaluate the healing of drying cracks in air-dried bentonite-sand blocks after hydration and swelling in groundwater,providing justifications to simplify the protection of blocks prior to installation in a high-level radioactive waste repository.Synthetic groundwater was prepared to represent the geochemistry of Beishan groundwater,and was used to hydrate the blocks during the swelling pressure and swelling strain measurements,as Beishan is the most promising site for China's repository.Healing of the surface cracks was recorded by photography,and healing of the internal cracks was visualized by CT images and hydraulic conductivity of air-dried blocks.The results indicate that the maximum swelling pressure and swelling strain are primarily affected by the geochemistry of Beishan groundwater,but not affected by the drying cracks.The maximum swelling pressure and swelling strain of air-dried blocks are comparable to or even higher than the pressure and strain of fresh blocks.The maximum swelling pressure measured in strong(i.e.high ion strength)Beishan groundwater was 44%of the pressure measured in deionized(DI)water,and the maximum swelling strain was reduced to 23%of the strain measured in DI water.Nevertheless,the remained swelling of the blocks hydrated in strong Beishan groundwater was sufficient to heal the surface and internal drying cracks,as demonstrated by the pictures of surface cracks and CT images.The hydraulic conductivity of the air-dried block permeated with strong groundwater was comparable(3.7×higher)to the hydraulic conductivity of the fresh block,indicating the self-healing of drying cracks after hydration and swelling in groundwater.A simplified method of protecting the block with plastic wraps before installation is recommended,since the remained swelling of the block hydrated in Beishan groundwater is sufficient to heal the drying cracks. 展开更多
关键词 Beishan groundwater chemistry Bentonite buffer Drying cracks high-level radioactive waste(HLW) SELF-HEALING SWELLING
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Corrosion techniques and strategies for used fuel containers with copper corrosion barriers under deep geological disposal conditions:A review
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作者 Yanxin Qiao Tianyu Wang +3 位作者 Zhilin Chen Jun Wang Chengtao Li Jian Chen 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第12期2582-2606,共25页
Safe emplacement of high-level nuclear waste(HLNW)arising from the utilization of nuclear power is a frequently en-countered and considerably challenging issue.The widely accepted and feasible approach for the permane... Safe emplacement of high-level nuclear waste(HLNW)arising from the utilization of nuclear power is a frequently en-countered and considerably challenging issue.The widely accepted and feasible approach for the permanent disposal of HLNW involves housing it in a corrosion-resistant container and subsequently burying it deep in a geologic repository.The focus lies on ensuring the dur-ability and integrity of the container in this process.This review introduces various techniques and strategies employed in controlling the corrosion of used fuel containers(UFCs)using copper(Cu)as corrosion barrier in the context of deep geological disposal.Overall,these corrosion prevention techniques and methods have been effectively implemented and employed to successfully mitigate the corrosion challenges encountered during the permanent disposal of Cu containers(e.g.,corrosion mechanisms and corrosion parameters)in deep geologic repositories.The primary objective of this review is to provide an extensive examination of the alteration in the corrosion envir-onment encountered by the UFCs when subjected to deep geologic repository conditions and focusing on addressing the potential corro-sion scenarios. 展开更多
关键词 corrosion prevention Cu SULPHIDE high-level nuclear waste disposal
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The Beishan underground research laboratory for geological disposal of high-level radioactive waste in China:Planning, site selection,site characterization and in situ tests 被引量:74
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作者 Ju Wang Liang Chen +1 位作者 Rui Su Xingguang Zhao 《Journal of Rock Mechanics and Geotechnical Engineering》 CSCD 2018年第3期411-435,共25页
With the rapid development of nuclear power in China, the disposal of high-level radioactive waste(HLW) has become an important issue for nuclear safety and environmental protection. Deep geological disposal is inte... With the rapid development of nuclear power in China, the disposal of high-level radioactive waste(HLW) has become an important issue for nuclear safety and environmental protection. Deep geological disposal is internationally accepted as a feasible and safe way to dispose of HLW, and underground research laboratories(URLs) play an important and multi-faceted role in the development of HLW repositories. This paper introduces the overall planning and the latest progress for China's URL. On the basis of the proposed strategy to build an area-specific URL in combination with a comprehensive evaluation of the site selection results obtained during the last 33 years, the Xinchang site in the Beishan area,located in Gansu Province of northwestern China, has been selected as the final site for China's first URL built in granite. In the process of characterizing the Xinchang URL site, a series of investigations,including borehole drilling,geological mapping, geophysical surveying,hydraulic testing and in situ stress measurements, has been conducted. The investigation results indicate that the geological,hydrogeological, engineering geological and geochemical conditions of the Xinchang site are very suitable for URL construction. Meanwhile, to validate and develop construction technologies for the Beishan URL, the Beishan exploration tunnel(BET), which is a 50-m-deep facility in the Jiujing sub-area, has been constructed and several in situ tests, such as drill-and-blast tests, characterization of the excavation damaged zone(EDZ), and long-term deformation monitoring of surrounding rocks, have been performed in the BET. The methodologies and technologies established in the BET will serve for URL construction.According to the achievements of the characterization of the URL site, a preliminary design of the URL with a maximum depth of 560 m is proposed and necessary in situ tests in the URL are planned. 展开更多
关键词 Beishan Xinchang site GRANITE Underground research laboratory(URL) high-level radioactive waste(HLW) Geological disposal
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High-level radioactive waste disposal in China: update 2010 被引量:39
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作者 Ju Wang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE 2010年第1期1-11,共11页
For geological disposal of high-level radioactive waste (HLW), the Chinese policy is that the spent nuclear fuel (SNF) should be reprocessed first, followed by vitrification and final disposal. The preliminary rep... For geological disposal of high-level radioactive waste (HLW), the Chinese policy is that the spent nuclear fuel (SNF) should be reprocessed first, followed by vitrification and final disposal. The preliminary repository concept is a shaft-tunnel model, located in saturated zones in granite, while the final waste form for disposal is vitrified high-level radioactive waste. In 2006, the government published a long-term research and development (R&D) plan for geological disposal of high-level radioactive waste. The program consists of three steps: (1) laboratory studies and site selection for a HLW repository (2006-2020); (2) underground in-situ tests (2021-2040); and (3) repository construction (2041-2050) followed by operation. With the support of China Atomic Energy Authority, comprehensive studies are underway and some progresses are made. The site characterization, including deep borehole drilling, has been performed at the most potential Beishan site in Gansu Province, Northwestern China. The data from geological and hydrogeological investigations, in-situ stress and permeability measurements of rock mass are presented in this paper. Engineered barrier studies are concentrated on the Gaomiaozi bentonite. A mock-up facility, which is used to study the thermo-hydro-mechano-chemical (THMC) properties of the bentonite, is under construction. Several projects on mechanical properties of Beishan granite are also underway. The key scientific challenges faced with HLW disposal are also discussed. 展开更多
关键词 geological disposal high-level radioactive waste R&D program site selection BENTONITE
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Three-dimensional numerical simulation of methane drainage by high-level drill holes in a lower protective coal seam with a “U” type face 被引量:7
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作者 Xingkui Liu Shuzhao Yang 《International Journal of Coal Science & Technology》 EI CAS 2014年第4期434-440,共7页
Different drill-hole positions may produce different drainage results in low protective coal seams.To investigate this possibility,a 3D stope model is established,which covers three kinds of drill holes.The FLUENT com... Different drill-hole positions may produce different drainage results in low protective coal seams.To investigate this possibility,a 3D stope model is established,which covers three kinds of drill holes.The FLUENT computational fluid mechanics software is used to solve the mass,momentum and species conservation equations of the model.The spatial distributions of oxygen and methane was obtained by calculations and the drainage results of different drill-hole positions were compared.The results show that,from top to bottom,methane dilution by oxygen weakens gradually from the intake to the return side,and methane tends to float;methane and oxygen distribute horizontally.The high-level crossing holes contribute to better methane drainage and a greater level of control.Around these holes,the methane density decreases dramatically and a "half circle"distribution is formed.The methane density decreases on the whole,but a proportion of the methane moves back to deep into the goaf.The research findings provide theoretical grounds for methane drainage. 展开更多
关键词 Low protective coal seam 3D stope model high-level drill Drainage effects
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Design and development of large-scale in-situ PRACLAY heater test and horizontal high-level radioactive waste disposal gallery seal test in Belgian HADES 被引量:6
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作者 X.L.Li W.Bastiaens +3 位作者 P.Van Marcke J.Verstricht G.J.Chen E.Weetjens 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE 2010年第2期103-110,共8页
In Belgium,the Boom clay was selected as a potential host formation for the disposal of high-level radioactive waste(HLW).To demonstrate the suitability of Boom clay for bearing thermal load induced by the HLW,a lar... In Belgium,the Boom clay was selected as a potential host formation for the disposal of high-level radioactive waste(HLW).To demonstrate the suitability of Boom clay for bearing thermal load induced by the HLW,a large-scale in-situ heater test,called PRACLAY heater test,will be conducted in the underground research laboratory(URL) in Mol.Owing to the limitations of the test(a short period of time compared with that considered in a real repository,different boundary conditions,etc.),the test is designed to simulate,in a conservative way,the most critical state and phenomena that could occur in the host rock.The PRACLAY gallery was excavated at the end of 2007;the heating phase will begin in 2010 and will last for at least 10 years.The PRACLAY gallery itself leaves an opportunity to study the possibilities of sealing a disposal drift in Boom clay and testing the feasibility of hydraulic cut-off of any preferential pathway to the main access gallery through the excavation damage zone(EDZ) and the lining with a seal in a horizontal drift(horizontal seal).Indeed,this is a generic problem for all deep geological disposal facilities for HLW.An annular seal made of compacted swelling bentonite will be installed in the front of the heated part of the PRACLAY gallery for these purposes.This paper provides detailed considerations on the thermo-hydro-mechanical(THM) boundary conditions for the design of the PRACLAY heater test and the seal test with the support of numerical calculations.It is believed that these important items considered in the PRACLAY heater test design also constitute key issues for the repository design.The outcome of the PRACLAY heater test will be an important milestone for the Belgian repository design. 展开更多
关键词 high-level radioactive waste(HLW) Boom clay PRACLAY heater test hydraulic cut-off thermo-hydro-mechanical(THM) boundary conditions scoping calculation
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Migration of Some Elements and Radionuclides across aGranite-Granite Contact Zone: A Natural Analoguefor Safe Disposal of High-Level Radwastes 被引量:4
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作者 LUO Xingzhang MIN Maozhong +4 位作者 ZHANG Guanghui LI Xianguo YANG Zhe ZHAI Liying Zhu Deling 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 1999年第4期418-428,共11页
Abstract Elements and natural radionuclides in the contact zone of two granites with different ages would migrate from one to the other because of the difference in their chemical contents and later water-rock interac... Abstract Elements and natural radionuclides in the contact zone of two granites with different ages would migrate from one to the other because of the difference in their chemical contents and later water-rock interactions. This migration could serve as an analogue for the near-field process of radwastes in a high-level radwaste deep geological disposal repository. 展开更多
关键词 high-level radwaste safe disposal granitic contact zone natural analogue
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Controlling mine pressure by subjecting high-level hard rock strata to ground fracturing 被引量:4
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作者 Rui Gao Tiejun Kuang +2 位作者 Yanqun Zhang Wenyang Zhang Chunyang Quan 《International Journal of Coal Science & Technology》 EI CAS CSCD 2021年第6期1336-1350,共15页
When mining extra-thick coal seams,the main cause of strong ground pressure are the high-level thick and hard strata,but as yet there is no active and effective control technology.This paper proposes the method of sub... When mining extra-thick coal seams,the main cause of strong ground pressure are the high-level thick and hard strata,but as yet there is no active and effective control technology.This paper proposes the method of subjecting hard roofs to ground fracturing,and physical simulation is used to study the control effect of ground fracturing on the strata structure and energy release.The results show that ground fracturing changes the structural characteristics of the strata and reduces the energy release intensity and the spatial extent of overburden movement,thereby exerting significant control on the ground pressure.The Datong mining area in China is selected as the engineering background.An engineering test was conducted on site by ground horizontal well fracturing,and a 20-m-thick hard rock layer located 110 m vertically above the coal seam was targeted as the fracturing layer.On-site microseismic monitoring shows that the crack propagation length is up to 216 m and the height is up to 50 m.On-site mine pressure monitoring shows that(1)the roadway deformation is reduced to 100 mm,(2)the periodic weighting characteristics of the hydraulic supports are not obvious,and(3)the ground pressure in the working face is controlled significantly,thereby showing that the ground fracturing is successful.Ground fracturing changed the breaking characteristics of the high-level hard strata,thereby helping to ameliorate the stress concentration in the stope and providing an effective control approach for hard rock. 展开更多
关键词 Ground fracturing high-level hard roof Breakage characteristics Pressure control Safety mining
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Main outcomes from in situ thermo-hydro-mechanical experiments programme to demonstrate feasibility of radioactive high-level waste disposal in the Callovo-Oxfordian claystone 被引量:4
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作者 G. Armand F. Bumbieler +3 位作者 N. Conil R. de la Vaissière J.-M. Bosgiraud M.-N. Vu 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2017年第3期33-45,共13页
In the context of radioactive waste disposal,an underground research laboratory(URL)is a facility in which experiments are conducted to demonstrate the feasibility of constructing and operating a radioactive waste dis... In the context of radioactive waste disposal,an underground research laboratory(URL)is a facility in which experiments are conducted to demonstrate the feasibility of constructing and operating a radioactive waste disposal facility within a geological formation.The Meuse/Haute-Marne URL is a sitespecific facility planned to study the feasibility of a radioactive waste disposal in the Callovo-Oxfordian(COx)claystone.The thermo-hydro-mechanical(THM)behaviour of the host rock is significant for the design of the underground nuclear waste disposal facility and for its long-term safety.The French National Radioactive Waste Management Agency(Andra)has begun a research programme aiming to demonstrate the relevancy of the French high-level waste(HLW)concept.This paper presents the programme implemented from small-scale(small diameter)boreholes to full-scale demonstration experiments to study the THM effects of the thermal transient on the COx claystone and the strategy implemented in this new programme to demonstrate and optimise current disposal facility components for HLW.It shows that the French high-level waste concept is feasible and working in the COx claystone.It also exhibits that,as for other plastic clay or claystone,heating-induced pore pressure increases and that the THM behaviour is anisotropic. 展开更多
关键词 In situ experiments Claystone Thermo-hydro-mechanical(THM) behaviour Research programme Radioactive high-level waste(HLW) DISPOSAL
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High-level disinfection of gastrointestinal endoscope reprocessing 被引量:15
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作者 King-Wah Chiu Lung-Sheng Lu Shue-Shian Chiou 《World Journal of Experimental Medicine》 2015年第1期33-35,共3页
High level disinfection(HLD) of the gastrointestinal(GI)endoscope is not simply a slogan, but rather is a form of experimental monitoring-based medicine. By definition,GI endoscopy is a semicritical medical device. He... High level disinfection(HLD) of the gastrointestinal(GI)endoscope is not simply a slogan, but rather is a form of experimental monitoring-based medicine. By definition,GI endoscopy is a semicritical medical device. Hence,such medical devices require major quality assurance for disinfection. And because many of these items are temperature sensitive, low-temperature chemical methods, such as liquid chemical germicide, must be used rather than steam sterilization. In summarizing guidelines for infection prevention and control for GI endoscopy, there are three important steps that must be highlighted: manual washing, HLD with automated endoscope reprocessor, and drying. Strict adherence to current guidelines is required because compared to any other medical device, the GI endoscope is associated with more outbreaks linked to inadequate cleaning or disinfecting during HLD. Both experimental evaluation on the surveillance bacterial cultures and in-use clinical results have shown that, the monitoring of the stringent processes to prevent and control infection is an essential component of the broader strategy to ensure the delivery of safe endoscopy services, because endoscope reprocessing is a multistep procedure involving numerous factors that can interfere with its efficacy. Based on our years of experience in the surveillance of culture monitoring of endoscopic reprocessing, we aim in this study to carefully describe what details require attention in the GI endoscopy disinfection and to share our experience so that patients can be provided with high quality and safe medical practices. Quality management encompasses all aspects of pre- and postprocedural care including the efficiency of the endoscopy unit and reprocessing area, as well as the endoscopic procedure itself. 展开更多
关键词 Automated endoscope reprocessor Bacterial culture Gastrointestinal endoscopy high-level disinfection Liquid chemical germicide
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Experimental study on the physico-mechanical properties of Tamusu mudstone — A potential host rock for high-level radioactive waste in Inner Mongolia of China 被引量:3
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作者 Chen Lu Hongdan Yu +1 位作者 Honghui Li Weizhong Chen 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2022年第6期1901-1909,共9页
Tamusu mudstone, located in Bayin Gobi Basin in Inner Mongolia of China, has been selected as a potential host rock for high-level radioactive waste(HLW) disposal in China. A series of tests has been carried out, incl... Tamusu mudstone, located in Bayin Gobi Basin in Inner Mongolia of China, has been selected as a potential host rock for high-level radioactive waste(HLW) disposal in China. A series of tests has been carried out, including X-ray diffraction(XRD) tests, scanning electron microscopy(SEM) tests, disintegration tests, permeability tests and triaxial compression tests, to estimate the physico-mechanical properties of Tamusu mudstone in this work. The mineral composition of Tamusu mudstone was analyzed and it was considered as a stable rock due to its low disintegration rate, i.e. approximately 0.11%after several wet/dry cycles. Based on the results of permeability test, it was found that Tamusu mudstone has a low permeability, with the magnitude of about 10—20m^(2). The low permeability makes the mudstone well prevent nuclide migration and diffusion, and might be influenced by temperature.The triaxial tests show that Tamusu mudstone is a stiff mudstone with high compressive strength, which means that the excavation disturbed zone would be smaller compared to other types of mudstone due to construction and operation of HLW repositories. Finally, the properties of Tamusu mudstone were compared with those of Opalinus clay, Callovo-Oxfordian(COx) argillite, and Boom clay to further discuss the possibility of using Tamusu mudstone as a potential nuclear waste disposal medium. 展开更多
关键词 Tamusu mudstone Physico-mechanical properties high-level radioactive waste(HLW)repository Disintegration test Permeability test
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High-level expression and purification of Plutella xylostella acetylcholinesterase in Pichia pastoris and its potential application 被引量:2
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作者 LIU Niu CHE Jun +2 位作者 LAI Duo WEN Jin-jun XU Han-hong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第6期1358-1366,共9页
The acetylcholinesterase 2(AChE2)cloned from Plutella xylostella was first successfully expressed in methylotrophic yeast Pichia pastoris GS115.One transformant with high-level expression of the recombinant AChE(rA... The acetylcholinesterase 2(AChE2)cloned from Plutella xylostella was first successfully expressed in methylotrophic yeast Pichia pastoris GS115.One transformant with high-level expression of the recombinant AChE(rAChE,23.2 U mL-1in supernatant)was selected by plating on increasing concentrations of antibiotic G418 and by using a simple and specific chromogenic reaction with indoxyl acetate as a substrate.The maximum production of r ACh E reached about 11.8 mg of the enzyme protein per liter of culture.The r ACh E was first precipitated with ammonium sulfate(50%saturation)and then purified with procainamide affinity column chromatography.The enzyme was purified 12.1-fold with a yield of 22.8%and a high specific activity of 448.3 U mg-1.It was sensitive to inhibition by methamidophos and pirimicarb,the calculated 50% inhibitory concentration(IC50)values of the two pesticides were 0.357 and 0.888 mg L-1,respectively,and the calculated 70% inhibitory concentration(IC70)values were 0.521 and 0.839 mg L-1,respectively.The results suggested that it has a potential application in the detection of pesticide residues. 展开更多
关键词 Plutella xylostella ACETYLCHOLINESTERASE Pichia pastoris high-level expression
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On area-specific underground research laboratory for geological disposal of high-level radioactive waste in China 被引量:19
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作者 Ju Wang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2014年第2期99-104,共6页
Underground research laboratories (URLs), including "generic URLs" and "site-specific URLs", are un- derground facilities in which characterisation, testing, technology development, and/or demonstration activiti... Underground research laboratories (URLs), including "generic URLs" and "site-specific URLs", are un- derground facilities in which characterisation, testing, technology development, and/or demonstration activities are carried out in support of the development of geological repositories for high-level radioactive waste (HLW) disposal. In addition to the generic URL and site-specific URL, a concept of "areaspecific URL", or the third type of URL, is proposed in this paper. It is referred to as the facility that is built at a site within an area that is considered as a potential area for HLW repository or built at a place near the future repository site, and may be regarded as a precursor to the development of a repository at the site. It acts as a "generic URL", but also acts as a "site-specific URL" to some extent. Considering the current situation in China, the most suitable option is to build an "area-specific URL" in Beishan area, the first priority region for China's high-level waste repository. With this strategy, the goal to build China's URL by 2020 mav be achieved, but the time left is limited. 展开更多
关键词 Underground research laboratory (URL)Area-specific URL high-level radioactive waste (HLW)Geological disposal
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Swab culture monitoring of automated endoscope reprocessors after high-level disinfection 被引量:1
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作者 Lung-Sheng Lu Yi-Chun Chiu +2 位作者 Ming-Tzung Lin Tsung-Hui Hu King-Wah Chiu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第14期1660-1663,共4页
AIM:To conduct a bacterial culture study for monitoring decontamination of automated endoscope reprocessors(AERs) after high-level disinfection(HLD).METHODS:From February 2006 to January 2011,authors conducted randomi... AIM:To conduct a bacterial culture study for monitoring decontamination of automated endoscope reprocessors(AERs) after high-level disinfection(HLD).METHODS:From February 2006 to January 2011,authors conducted randomized consecutive sampling each month for 7 AERs.Authors collected a total of 420 swab cultures,including 300 cultures from 5 gastroscope AERs,and 120 cultures from 2 colonoscope AERs.Swab cultures were obtained from the residual water from the AERs after a full reprocessing cycle.Samples were cultured to test for aerobic bacteria,anaerobic bacteria,and mycobacterium tuberculosis.RESULTS:The positive culture rate of the AERs was 2.0%(6/300) for gastroscope AERs and 0.8%(1/120) for colonoscope AERs.All the positive cultures,including 6 from gastroscope and 1 from colonoscope AERs,showed monofloral colonization.Of the gastroscopeAER samples,50%(3/6) were colonized by aerobic bacterial and 50%(3/6) by fungal contaminations.CONCLUSION:A full reprocessing cycle of an AER with HLD is adequate for disinfection of the machine.Swab culture is a useful method for monitoring AER decontamination after each reprocessing cycle.Fungal contamination of AERs after reprocessing should also be kept in mind. 展开更多
关键词 Automated endoscope reprocessor Gastrointestinal scope high-level disinfection Swab culture MONITORING DECONTAMINATION
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Corrosion behavior of high-level waste container materials Ti and Ti-Pd alloy under long-term gamma irradiation in Beishan groundwater 被引量:1
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作者 Qianglin Wei Yuhong Li +3 位作者 Yanliang Huang Dongyan Yang Bo Yang Yibao Liu 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第5期93-100,共8页
Titanium and titanium-palladium alloys are important potential materials for nuclear waste container,which will endure both intenseγ-irradiation and groundwater erosion.Therefore,it is very important to investigate t... Titanium and titanium-palladium alloys are important potential materials for nuclear waste container,which will endure both intenseγ-irradiation and groundwater erosion.Therefore,it is very important to investigate the corrosion behavior of the container materials.In this research,the cumulative dose effect of TA8-1 type titanium-palladium alloy(TA8-1)and TA2-type pure titanium(TA2)underγ-irradiation was studied based on the geological disposal of nuclear wastes.The irradiation experiments were performed at room temperature using^(60)Co gamma sources with a 5.0-kGy·h^(-1)intensity for 40,80 or 160 days,respectively.The pH value and conductivity of Beishan groundwater were investigated.The results showed that the pH value changed from alkaline(8.22)to acidic(2.46 for TA8-1 and 2.44 for TA2),while the un-irradiated solution remained alkaline(8.17 for TA8-1 and 8.20 for TA2)after 160 days.With the increase of irradiation dose,the conductivity increases rapidly and then tends to become stable,which indicates that the titanium dioxide corrosion layer formed on the surface of the sample surface effectively prevents further corrosion.Meanwhile,XRD and SEM-EDS analysis results show that the main components of corrosion products are TiO_(2) and TiO.The titanium on the surface of the sample is oxidized,resulting in slight uneven local corrosion.The results show that TA8-1 and TA2 are suitable to be used as candidate materials for high-level waste(HLW)disposal containers due to their excellent performance under long-term and high-dose irradiation corrosion. 展开更多
关键词 Γ-IRRADIATION CORROSION high-level waste container material
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A novel quantum information hiding protocol based on entanglement swapping of high-level Bell states 被引量:1
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作者 徐淑奖 陈秀波 +2 位作者 王连海 钮心忻 杨义先 《Chinese Physics B》 SCIE EI CAS CSCD 2015年第5期231-238,共8页
Using entanglement swapping of high-level Bell states, we first derive a covert layer between the secret message and the possible output results of the entanglement swapping between any two generalized Bell states, an... Using entanglement swapping of high-level Bell states, we first derive a covert layer between the secret message and the possible output results of the entanglement swapping between any two generalized Bell states, and then propose a novel high-efficiency quantum information hiding protocol based on the covert layer. In the proposed scheme, a covert channel can be built up under the cover of a high-level quantum secure direct communication (QSDC) channel for securely transmitting secret messages without consuming any auxiliary quantum state or any extra communication resource. It is shown that this protocol not only has a high embedding efficiency but also achieves a good imperceptibility as well as a high security. 展开更多
关键词 quantum information hiding quantum covert'channel entanglement swapping high-level Bell states
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