建立了采用Oasis PRiME HLB固相萃取柱净化、超高效液相色谱-串联质谱法同时测定水产品中8种磺胺类药物残留量的检测方法。该方法的流程为样品经甲酸化乙腈提取,通过Oasis PRiME HLB固相萃取柱净化,高效液相色谱-串联质谱法测定,内标法...建立了采用Oasis PRiME HLB固相萃取柱净化、超高效液相色谱-串联质谱法同时测定水产品中8种磺胺类药物残留量的检测方法。该方法的流程为样品经甲酸化乙腈提取,通过Oasis PRiME HLB固相萃取柱净化,高效液相色谱-串联质谱法测定,内标法定量。该方法在0.01~0.20μg/mL范围内线性良好,8种磺胺类药物相关系数均大于0.997。测定时添加浓度分别为2μg/kg和10μg/kg的加标样品,回收率均在70%~120%,方法准确性良好。展开更多
Traditional Chinese medicine(TCM)belongs to the most elaborate and extensive systems of plant-based healing.The herb Northern Ban Lan(Isatis tinctoria)is famous for its antiviral and anti-inflammatory activity.Althoug...Traditional Chinese medicine(TCM)belongs to the most elaborate and extensive systems of plant-based healing.The herb Northern Ban Lan(Isatis tinctoria)is famous for its antiviral and anti-inflammatory activity.Although numerous components isolated from I.tinctoria have been characterized so far,their modes of action have remained unclear.Here,we show that extracts from I.tinctoria exert antimicrotubular activity.Using time-lapse microscopy in living tobacco BY-2(Nicotiana tabacum L.cvBrightYellow2)cellsexpressing greenfluorescentprotein-tubulin,weuse activity-guided fractionation to screen out the biologically active compounds of I.tinctoria.Among 54 fractions obtained from either leaves or roots of I.tinctoria by methanol(MeOH/H_(2)O 8:2),or ethyl acetate extraction,one specific methanolic root fraction was selected,because it efficiently and rapidly eliminated microtubules.By combination of further purification with ultrahigh-performance liquid chromatography and high-resolution tandem mass spectrometry most of the bioactivity could be assigned to the glucosinolate compound glucobrassicin.Glucobrassicin can also affect microtubules and induce apoptosis in HeLa cells.In the light of these findings,the antiviral activity of Northern Ban Lan is discussed in the context of microtubules being hijacked by many viral pathogens for cell-to-cell spread.展开更多
文摘建立了采用Oasis PRiME HLB固相萃取柱净化、超高效液相色谱-串联质谱法同时测定水产品中8种磺胺类药物残留量的检测方法。该方法的流程为样品经甲酸化乙腈提取,通过Oasis PRiME HLB固相萃取柱净化,高效液相色谱-串联质谱法测定,内标法定量。该方法在0.01~0.20μg/mL范围内线性良好,8种磺胺类药物相关系数均大于0.997。测定时添加浓度分别为2μg/kg和10μg/kg的加标样品,回收率均在70%~120%,方法准确性良好。
基金supported by fellowships from the China Scholarship Council。
文摘Traditional Chinese medicine(TCM)belongs to the most elaborate and extensive systems of plant-based healing.The herb Northern Ban Lan(Isatis tinctoria)is famous for its antiviral and anti-inflammatory activity.Although numerous components isolated from I.tinctoria have been characterized so far,their modes of action have remained unclear.Here,we show that extracts from I.tinctoria exert antimicrotubular activity.Using time-lapse microscopy in living tobacco BY-2(Nicotiana tabacum L.cvBrightYellow2)cellsexpressing greenfluorescentprotein-tubulin,weuse activity-guided fractionation to screen out the biologically active compounds of I.tinctoria.Among 54 fractions obtained from either leaves or roots of I.tinctoria by methanol(MeOH/H_(2)O 8:2),or ethyl acetate extraction,one specific methanolic root fraction was selected,because it efficiently and rapidly eliminated microtubules.By combination of further purification with ultrahigh-performance liquid chromatography and high-resolution tandem mass spectrometry most of the bioactivity could be assigned to the glucosinolate compound glucobrassicin.Glucobrassicin can also affect microtubules and induce apoptosis in HeLa cells.In the light of these findings,the antiviral activity of Northern Ban Lan is discussed in the context of microtubules being hijacked by many viral pathogens for cell-to-cell spread.
