With the development of economy and society and the growth of population,the high-rise and multi-function of commercial buildings have become an international trend.But it also poses huge fire hazards.Most of the exis...With the development of economy and society and the growth of population,the high-rise and multi-function of commercial buildings have become an international trend.But it also poses huge fire hazards.Most of the existing studies’research objects are predominantly high-rise residential buildings,without considering the impact of different functional zones(Standard floor,entertainment zone,office zone,equipment room and so on)and personnel distribution of commercial buildings evacuation.And the influence of using elevators to carry evacuees on the refuge floor on personnel evacuation is rarely studied.In this work,the fire scenario of the Yangtze River InternationalConferenceCenter,a high-rise commercial building,is simulated with the Pyrosim programto get the necessary parameters under various fire scenarios and to calculate the available evacuation time TASET.At the same time,according to the complex functional zone of the commercial high-rise building and the distribution of people in different time periods,a reasonable evacuation strategy is developed and simulated by Pathfinder software.The results indicate that unorganized evacuation will lead individuals to take the erroneous evacuation route,resulting in a vast region of congestion;comprehensive consideration of the time staggering and the reasonable distribution of evacuation routes can significantly improve evacuation efficiency,and the TRSET of night and working hours is 36.6%–55.3%and 49.9%–79.6%of unorganized evacuation,respectively.For the night fire,60%of the people use elevator-refuge floor to evacuate is the optimal strategy;for the fire during working hours,half of the people on standard floors use the elevator to evacuate and people on multifunctional floors evacuate in four batches is the best plan.The results of this study can provide viable solutions and a foundation for analyzing the fire evacuation and safety of big commercial high-rise buildings.展开更多
Collisions between a moving mass and an anti-collision device increase structural responses and threaten structural safety.An active mass damper(AMD)with stroke limitations is often used to avoid collisions.However,a ...Collisions between a moving mass and an anti-collision device increase structural responses and threaten structural safety.An active mass damper(AMD)with stroke limitations is often used to avoid collisions.However,a strokelimited AMD control system with a fixed limited area shortens the available AMD stroke and leads to significant control power.To solve this problem,the design approach with variable gain and limited area(VGLA)is proposed in this study.First,the boundary of variable-limited areas is calculated based on the real-time status of the moving mass.The variable gain(VG)expression at the variable limited area is deduced by considering the saturation of AMD stroke.Then,numerical simulations of a stroke-limited AMD control system with VGLA are conducted on a high-rise building structure.These numerical simulations show that the proposed approach has superior strokelimitation performance compared with a stroke-limited AMD control system with a fixed limited area.Finally,the proposed approach is validated through experiments on a four-story steel frame.展开更多
To enable the experimental assessment of the seismic performance of full-scale nonstructural elements with multiple engineering parameters(EDPs),a three-layer testbed named Nonstructural Element Simulator on Shake Tab...To enable the experimental assessment of the seismic performance of full-scale nonstructural elements with multiple engineering parameters(EDPs),a three-layer testbed named Nonstructural Element Simulator on Shake Table(NEST)has been developed.The testbed consists of three consecutive floors of steel structure.The bottom two floors provide a space to accommodate a full-scale room.To fully explore the flexibility of NEST,we propose a novel control strategy to generate the required shake table input time histories for the testbed to track the target floor motions of the buildings of interest with high accuracy.The control strategy contains two parts:an inverse dynamic compensation via simulation of feedback control systems(IDCS)algorithm and an offline iteration procedure based on a refined nonlinear numerical model of the testbed.The key aspects of the control strategy were introduced in this paper.Experimental tests were conducted to simulate the seismic responses of a full-scale office room on the 21^(st)floor of a 42-story high-rise building.The test results show that the proposed control strategy can reproduce the target floor motions of the building of interest with less than 20%errors within the specified frequency range.展开更多
Many modern Chinese libraries have established distribution agencies in other cities to distribute books published or sold on consignment.Huating Bookstore,which was once the Shanghai General Distribution Office of Zh...Many modern Chinese libraries have established distribution agencies in other cities to distribute books published or sold on consignment.Huating Bookstore,which was once the Shanghai General Distribution Office of Zhejiang Library in modern times,is one of them.On the basis of a brief description of modern Zhejiang Library and its book publishing,as well as the overview of Huating Bookstore,the paper introduces and analyzes the ZPL Publishing Book Catalogue sent by Huating Bookstore,and the ZPL publishing and selling consignment books issued by Huating Bookstore.It points out that Huating Bookstore is a bridge between the ZPL located in Hangzhou and various retail ZPL publishing bookstores in Shanghai.Their production and sales relationship is a mutually beneficial one.展开更多
Cost overrun is a common problem in construction projects worldwide.Most Indian construction projects,particularly those involving high-rise buildings,have had severe cost overruns.For managers,architects,engineers,an...Cost overrun is a common problem in construction projects worldwide.Most Indian construction projects,particularly those involving high-rise buildings,have had severe cost overruns.For managers,architects,engineers,and contractors,completing building projects within the specified cost budget has become the most important and hard assignment.Since it is common for high-rise building projects to go over budget,the aim of this study is to find out the causes of cost overruns and provide effective measures.The study found 70 cost overrun factors based on a comprehensive literature review and expert opinions.A Google form questionnaire was distributed to 150 construction professionals across India.After following up,101 of the 150 responses were received.A five-point Likert scale was used and the acquired data was analyzed and ranked using the Relative Importance Index(RII)technique.According to the findings of RII,the top ten critical factors influencing cost overruns were frequent change orders during construction by the owner,delay in construction,escalation of material prices,market inflation or deflation,rework,frequent changes in design,inaccurate evaluation of the project timeline,unforeseen ground condition,inaccurate quantity take-off,and delay in progressive payment by the owner.Spearman’s rank correlation test revealed that there is a very significant relationship between the rankings of factors provided by the owner,the consultant,and the contractor.In addition,a factor analysis tool in the SPSS software was also used to categorize the seventy factors into sixteen core components.The top ten critical factors were presented to subject matter experts,and their suggestions were being compiled.These results are expected to help construction professionals minimize cost overruns,improve cost control measures,and initiate future research.展开更多
The sol-gel method is used to prepare a new nano-alumina aerogel structure and the thermal properties of this nanomaterial are investigated comprehensively using electron microscope scanning,thermal analysis,X-ray and...The sol-gel method is used to prepare a new nano-alumina aerogel structure and the thermal properties of this nanomaterial are investigated comprehensively using electron microscope scanning,thermal analysis,X-ray and infrared spectrometer analysis methods.It is found that the composite aerogel alumina material has a multi-level porous nano-network structure.When employed for the thermal insulation of high-rise buildings,the alumina nanocomposite aerogel material can lead to effective energy savings in winter.However,it has almost no energy-saving effect on buildings where energy is consumed for cooling in summer.展开更多
A probabilistic seismic loss assessment of RC high-rise(RCHR)buildings designed according to Eurocode 8 and located in the Southern Euro-Mediterranean zone is presented herein.The loss assessment methodology is based ...A probabilistic seismic loss assessment of RC high-rise(RCHR)buildings designed according to Eurocode 8 and located in the Southern Euro-Mediterranean zone is presented herein.The loss assessment methodology is based on a comprehensive simulation approach which takes into account ground motion(GM)uncertainty,and the random effects in seismic demand,as well as in predicting the damage states(DSs).The methodology is implemented on three RCHR buildings of 20-story,30-story and 40-story with a core wall structural system.The loss functions described by a cumulative lognormal probability distribution are obtained for two intensity levels for a large set of simulations(NLTHAs)based on 60 GM records with a wide range of magnitude(M),distance to source(R)and different site soil conditions(SS).The losses expressed in percent of building replacement cost for RCHR buildings are obtained.In the estimation of losses,both structural(S)and nonstructural(NS)damage for four DSs are considered.The effect of different GM characteristics(M,R and SS)on the obtained losses are investigated.Finally,the estimated performance of the RCHR buildings are checked to ensure that they fulfill limit state requirements according to Eurocode 8.展开更多
Because of urbanization,land resources in China’s cities has become increasingly scarce.Therefore,modern buildings are becoming taller,making high-rise steel frame structures the new favorite of the construction indu...Because of urbanization,land resources in China’s cities has become increasingly scarce.Therefore,modern buildings are becoming taller,making high-rise steel frame structures the new favorite of the construction industry.However,the construction of high-rise steel frame structures requires advanced technology.If the construction technology is effectively implemented and the welding techniques of the construction personnel align with the requirements for high-rise steel frame structures,it can help mitigate deformations in the steel structure,thus preserving the overall construction quality of high-rise steel frame structures.To enhance the applicability of steel frame structures in high-rise buildings,this paper focuses on analyzing the optimization path for the construction process of high-rise steel frame structures.It introduces a tailored approach to control welding-induced deformations in steel frame structures,aiming to make a valuable contribution to the advancement of China’s construction industry.展开更多
Insertional mutation,phenotypic evaluation,and mutated gene cloning are widely used to clone genes from scratch.Exogenous genes can be integrated into the genome during non-homologous end joining(NHEJ)of the double-st...Insertional mutation,phenotypic evaluation,and mutated gene cloning are widely used to clone genes from scratch.Exogenous genes can be integrated into the genome during non-homologous end joining(NHEJ)of the double-strand breaks of DNA,causing insertional mutation.The random insertional mutant library constructed using this method has become a method of forward genetics for gene cloning.However,the establishment of a random insertional mutant library requires a high transformation efficiency of exogenous genes.Many microalgal species show a low transformation efficiency,making constructing random insertional mutant libraries difficult.In this study,we established a highly efficient transformation method for constructing a random insertional mutant library of Nannochloropsis oceanica,and tentatively tried to isolate its genes to prove the feasibility of the method.A gene that may control the growth rate and cell size was identified.This method will facilitate the genetic studies of N.oceanica,which should also be a reference for other microalgal species.展开更多
Molecular Biology and Experiment is considered fundamental for graduate students specializing in aquaculture at Guangdong Ocean University.This discipline focuses on the examination of the structure and function of ma...Molecular Biology and Experiment is considered fundamental for graduate students specializing in aquaculture at Guangdong Ocean University.This discipline focuses on the examination of the structure and function of macromolecules,including proteins and nucleic acids.Moreover,it elucidates biological phenomena and principles at the molecular level,making it an essential foundational course for students pursuing various biology majors.As a foundational course for the basic application of aquaculture,Molecular Biology and Experiment requires guidance through numerous examples and cases.However,there are several challenges to address in developing the case library.Consequently,a case library has been established to meet the course requirements of Molecular Biology and Experiment for modern graduate students,with the central goal of reforming the educational model of higher education institutions and enhancing the effectiveness and quality of talent development.This strategy is designed to nurture highly skilled professionals who can address the current needs of the industry.展开更多
The paper is based on literature search,introducing and analyzing the book publishing situation of modern Peking University Library from the perspective of the publisher being Peking University Library and its predece...The paper is based on literature search,introducing and analyzing the book publishing situation of modern Peking University Library from the perspective of the publisher being Peking University Library and its predecessor.It is pointed out that the majority of the book printing methods published by the modern Peking University Library are lead printing,and among the types of Chinese library classification,catalogue of library collections is the most common.展开更多
Building occupants are not immune to ill-health as a result of time they spend in a building.This paper seeks to examine the effects furniture ergonomics have on student’s satisfaction in the library of Universiti Te...Building occupants are not immune to ill-health as a result of time they spend in a building.This paper seeks to examine the effects furniture ergonomics have on student’s satisfaction in the library of Universiti Teknologi Malaysia.A pilot survey was initially conducted in the library through a one-to-one interaction with students to fetch their opinions on the general effects of the furniture.An observation through several walkthroughs was also conducted by the researchers to compare and validate responses obtained.Two hundred and sixty-five students that come from fifteen nationalities are surveyed.A structured questionnaire is used to collect data on the respondents’opinions on the size,shape,arrangement and comfort of the furniture.Eta cross tabulation,Spearman’s rho and Kendall’s Tau-b are used to establish relationships.Results show that amongst the effects studied,there are significant positive relationships between students’satisfaction of furniture ergonomics as against back-strain and lack of concentration.This implies that the more the furniture arrangements,size and shape are perceived unsatisfactory,the more their effects on back-strain and lack of concentration towards the students.This paper further recommends that library management should see to designing IEQ(Indoor Environmental Quality)guidelines that will mitigate the effects of furniture ergonomics thus improving student’s satisfaction.展开更多
The library seating management system is an important part of the library’s modern services. The system is based on the Spring and Spring Boot framework, and the system realizes a series of functions such as real-tim...The library seating management system is an important part of the library’s modern services. The system is based on the Spring and Spring Boot framework, and the system realizes a series of functions such as real-time query, reservation, and cancellation of seat resources, providing users with great convenience. With a simple operation, users can know the availability of seats in the library in real time and reserve them according to their needs. At the same time, the system also provides rich management functions, enabling administrators to easily configure and manage seat resources. The addition, deletion, modification and review of users, the generation of seats, the viewing of user usage records, and the addition or deletion of points for users’ usage can also be carried out. This not only improves the management efficiency, but also provides more scientific and accurate data support for the management of the library. The system not only optimizes the user experience, but also promotes the scientific management and efficient utilization of library resources, and provides strong support for the modern services of the library.展开更多
In the contemporary digital age,traditional reading habits have been compromised by digital media,a situation that has forced academic libraries to adopt proactive marketing approaches to promote reading among student...In the contemporary digital age,traditional reading habits have been compromised by digital media,a situation that has forced academic libraries to adopt proactive marketing approaches to promote reading among students and faculty.This article investigates the implementation of marketing strategies in promoting reading activities within academic libraries,taking the University of Shanghai for Science and Technology as a case study.The paper explores various marketing strategies employed by academic libraries,including market segmentation,utilization of new media platforms,and the creation of personalized content for readers.Drawing from an extensive literature review and real-world examples,the study establishes a framework for marketing reading promotion activities tailored to academic libraries.These findings underscore how effective marketing strategies can enhance user participation,expand library influence,and optimize resource utilization.展开更多
To enhance our talent cultivation model through“school-enterprise cooperation and industry-teaching fusion”,we aim to improve the“4321”Industry-Education Integration System.This includes actively promoting the use...To enhance our talent cultivation model through“school-enterprise cooperation and industry-teaching fusion”,we aim to improve the“4321”Industry-Education Integration System.This includes actively promoting the use of case banks and project banks in teaching to develop students’practical engineering skills through hands-on application of professional knowledge.Additionally,landscape design courses emphasize practical learning experiences to implement the fundamental goal of“cultivating morality”.Guided by enhancing students’practical skills,we ensure alignment with course objectives and professional training requirements,emphasizing the seamless integration of theory and practice.展开更多
University libraries,as an integral component of university research capabilities,are tasked with serving education by providing research support to faculty and students.This study adopts an affordance research perspe...University libraries,as an integral component of university research capabilities,are tasked with serving education by providing research support to faculty and students.This study adopts an affordance research perspective originating from ecological psychology to explore the optimization path of research support services in libraries in the new media era.Based on the characteristics of university library services,this research analyzes three aspects:production affordance,mobile affordance,and social affordance,and proposes practical recommendations to guide future research service practices of university libraries.展开更多
Based on several bibliographic advertisements carried in newspapers and magazines from 1919 to 1922 for books published by Peking University Publishing Department,and issued by Yadong Library,the paper analyzes the ch...Based on several bibliographic advertisements carried in newspapers and magazines from 1919 to 1922 for books published by Peking University Publishing Department,and issued by Yadong Library,the paper analyzes the characteristics of the books published by Peking University Publishing Department during this period,and points out that Yadong Library entered its golden period of development due to move from the alley to the main road and obtain the management rights of the books published by Peking University Publishing Department.展开更多
Since the age of digital intelligence,the government has introduced policies to actively promote the intelligent transformation of grassroots public cultural services.Based on the investigation and analysis of the dev...Since the age of digital intelligence,the government has introduced policies to actively promote the intelligent transformation of grassroots public cultural services.Based on the investigation and analysis of the development status quo of grassroots libraries in Henan Province,we put forward the path of high-quality development of grassroots libraries in Henan,namely,improving the mechanism and system,leading with digital intelligence technology,empowering by Yellow River culture(the heritage and values rooted in the history and traditions of the Yellow River region),and driven by users’demand.展开更多
基金the grant fromthe Key Technologies Research and Development Program(Grant No.2021YFF0602005)the National Natural Science Foundation of China(No.51678135)the Fundamental Research Funds for the Central Universities(Nos.2242022k30031,2242022k30033).
文摘With the development of economy and society and the growth of population,the high-rise and multi-function of commercial buildings have become an international trend.But it also poses huge fire hazards.Most of the existing studies’research objects are predominantly high-rise residential buildings,without considering the impact of different functional zones(Standard floor,entertainment zone,office zone,equipment room and so on)and personnel distribution of commercial buildings evacuation.And the influence of using elevators to carry evacuees on the refuge floor on personnel evacuation is rarely studied.In this work,the fire scenario of the Yangtze River InternationalConferenceCenter,a high-rise commercial building,is simulated with the Pyrosim programto get the necessary parameters under various fire scenarios and to calculate the available evacuation time TASET.At the same time,according to the complex functional zone of the commercial high-rise building and the distribution of people in different time periods,a reasonable evacuation strategy is developed and simulated by Pathfinder software.The results indicate that unorganized evacuation will lead individuals to take the erroneous evacuation route,resulting in a vast region of congestion;comprehensive consideration of the time staggering and the reasonable distribution of evacuation routes can significantly improve evacuation efficiency,and the TRSET of night and working hours is 36.6%–55.3%and 49.9%–79.6%of unorganized evacuation,respectively.For the night fire,60%of the people use elevator-refuge floor to evacuate is the optimal strategy;for the fire during working hours,half of the people on standard floors use the elevator to evacuate and people on multifunctional floors evacuate in four batches is the best plan.The results of this study can provide viable solutions and a foundation for analyzing the fire evacuation and safety of big commercial high-rise buildings.
基金This research was founded by the Funds for Creative Research Groups of National Natural Science Foundation of China(Grant No.51921006)the National Natural Science Foundations of China(Grant No.51978224)+2 种基金the National Major Scientific Research Instrument Development Program of China(Grant No.51827811)the National Natural Science Foundation of China,(Grant No.52008141)the Shenzhen Technology Innovation Program(Grant Nos.JCYJ20170811160003571,JCYJ20180508152238111 and JCYJ20200109112803851).
文摘Collisions between a moving mass and an anti-collision device increase structural responses and threaten structural safety.An active mass damper(AMD)with stroke limitations is often used to avoid collisions.However,a strokelimited AMD control system with a fixed limited area shortens the available AMD stroke and leads to significant control power.To solve this problem,the design approach with variable gain and limited area(VGLA)is proposed in this study.First,the boundary of variable-limited areas is calculated based on the real-time status of the moving mass.The variable gain(VG)expression at the variable limited area is deduced by considering the saturation of AMD stroke.Then,numerical simulations of a stroke-limited AMD control system with VGLA are conducted on a high-rise building structure.These numerical simulations show that the proposed approach has superior strokelimitation performance compared with a stroke-limited AMD control system with a fixed limited area.Finally,the proposed approach is validated through experiments on a four-story steel frame.
基金supported by the Natural Science Foundation of China(52122811)。
文摘To enable the experimental assessment of the seismic performance of full-scale nonstructural elements with multiple engineering parameters(EDPs),a three-layer testbed named Nonstructural Element Simulator on Shake Table(NEST)has been developed.The testbed consists of three consecutive floors of steel structure.The bottom two floors provide a space to accommodate a full-scale room.To fully explore the flexibility of NEST,we propose a novel control strategy to generate the required shake table input time histories for the testbed to track the target floor motions of the buildings of interest with high accuracy.The control strategy contains two parts:an inverse dynamic compensation via simulation of feedback control systems(IDCS)algorithm and an offline iteration procedure based on a refined nonlinear numerical model of the testbed.The key aspects of the control strategy were introduced in this paper.Experimental tests were conducted to simulate the seismic responses of a full-scale office room on the 21^(st)floor of a 42-story high-rise building.The test results show that the proposed control strategy can reproduce the target floor motions of the building of interest with less than 20%errors within the specified frequency range.
基金the research results of Humanities and Social Science Planning Fund Project of the Ministry of Education of P.R.China,titled“Research on the Books Publishing of Modern Chinese Library from the Perspective of Generalized Technology”(Project number:19YJA870014).
文摘Many modern Chinese libraries have established distribution agencies in other cities to distribute books published or sold on consignment.Huating Bookstore,which was once the Shanghai General Distribution Office of Zhejiang Library in modern times,is one of them.On the basis of a brief description of modern Zhejiang Library and its book publishing,as well as the overview of Huating Bookstore,the paper introduces and analyzes the ZPL Publishing Book Catalogue sent by Huating Bookstore,and the ZPL publishing and selling consignment books issued by Huating Bookstore.It points out that Huating Bookstore is a bridge between the ZPL located in Hangzhou and various retail ZPL publishing bookstores in Shanghai.Their production and sales relationship is a mutually beneficial one.
文摘Cost overrun is a common problem in construction projects worldwide.Most Indian construction projects,particularly those involving high-rise buildings,have had severe cost overruns.For managers,architects,engineers,and contractors,completing building projects within the specified cost budget has become the most important and hard assignment.Since it is common for high-rise building projects to go over budget,the aim of this study is to find out the causes of cost overruns and provide effective measures.The study found 70 cost overrun factors based on a comprehensive literature review and expert opinions.A Google form questionnaire was distributed to 150 construction professionals across India.After following up,101 of the 150 responses were received.A five-point Likert scale was used and the acquired data was analyzed and ranked using the Relative Importance Index(RII)technique.According to the findings of RII,the top ten critical factors influencing cost overruns were frequent change orders during construction by the owner,delay in construction,escalation of material prices,market inflation or deflation,rework,frequent changes in design,inaccurate evaluation of the project timeline,unforeseen ground condition,inaccurate quantity take-off,and delay in progressive payment by the owner.Spearman’s rank correlation test revealed that there is a very significant relationship between the rankings of factors provided by the owner,the consultant,and the contractor.In addition,a factor analysis tool in the SPSS software was also used to categorize the seventy factors into sixteen core components.The top ten critical factors were presented to subject matter experts,and their suggestions were being compiled.These results are expected to help construction professionals minimize cost overruns,improve cost control measures,and initiate future research.
文摘The sol-gel method is used to prepare a new nano-alumina aerogel structure and the thermal properties of this nanomaterial are investigated comprehensively using electron microscope scanning,thermal analysis,X-ray and infrared spectrometer analysis methods.It is found that the composite aerogel alumina material has a multi-level porous nano-network structure.When employed for the thermal insulation of high-rise buildings,the alumina nanocomposite aerogel material can lead to effective energy savings in winter.However,it has almost no energy-saving effect on buildings where energy is consumed for cooling in summer.
文摘A probabilistic seismic loss assessment of RC high-rise(RCHR)buildings designed according to Eurocode 8 and located in the Southern Euro-Mediterranean zone is presented herein.The loss assessment methodology is based on a comprehensive simulation approach which takes into account ground motion(GM)uncertainty,and the random effects in seismic demand,as well as in predicting the damage states(DSs).The methodology is implemented on three RCHR buildings of 20-story,30-story and 40-story with a core wall structural system.The loss functions described by a cumulative lognormal probability distribution are obtained for two intensity levels for a large set of simulations(NLTHAs)based on 60 GM records with a wide range of magnitude(M),distance to source(R)and different site soil conditions(SS).The losses expressed in percent of building replacement cost for RCHR buildings are obtained.In the estimation of losses,both structural(S)and nonstructural(NS)damage for four DSs are considered.The effect of different GM characteristics(M,R and SS)on the obtained losses are investigated.Finally,the estimated performance of the RCHR buildings are checked to ensure that they fulfill limit state requirements according to Eurocode 8.
文摘Because of urbanization,land resources in China’s cities has become increasingly scarce.Therefore,modern buildings are becoming taller,making high-rise steel frame structures the new favorite of the construction industry.However,the construction of high-rise steel frame structures requires advanced technology.If the construction technology is effectively implemented and the welding techniques of the construction personnel align with the requirements for high-rise steel frame structures,it can help mitigate deformations in the steel structure,thus preserving the overall construction quality of high-rise steel frame structures.To enhance the applicability of steel frame structures in high-rise buildings,this paper focuses on analyzing the optimization path for the construction process of high-rise steel frame structures.It introduces a tailored approach to control welding-induced deformations in steel frame structures,aiming to make a valuable contribution to the advancement of China’s construction industry.
基金the National Key R&D Program of China(Nos.2018YFD0901506,2018YFD0900305)the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018 SDKJ0406-3)。
文摘Insertional mutation,phenotypic evaluation,and mutated gene cloning are widely used to clone genes from scratch.Exogenous genes can be integrated into the genome during non-homologous end joining(NHEJ)of the double-strand breaks of DNA,causing insertional mutation.The random insertional mutant library constructed using this method has become a method of forward genetics for gene cloning.However,the establishment of a random insertional mutant library requires a high transformation efficiency of exogenous genes.Many microalgal species show a low transformation efficiency,making constructing random insertional mutant libraries difficult.In this study,we established a highly efficient transformation method for constructing a random insertional mutant library of Nannochloropsis oceanica,and tentatively tried to isolate its genes to prove the feasibility of the method.A gene that may control the growth rate and cell size was identified.This method will facilitate the genetic studies of N.oceanica,which should also be a reference for other microalgal species.
基金Supported by Degree and Graduate Student Education Reform Research Project of Guangdong Ocean University(202315,202416).
文摘Molecular Biology and Experiment is considered fundamental for graduate students specializing in aquaculture at Guangdong Ocean University.This discipline focuses on the examination of the structure and function of macromolecules,including proteins and nucleic acids.Moreover,it elucidates biological phenomena and principles at the molecular level,making it an essential foundational course for students pursuing various biology majors.As a foundational course for the basic application of aquaculture,Molecular Biology and Experiment requires guidance through numerous examples and cases.However,there are several challenges to address in developing the case library.Consequently,a case library has been established to meet the course requirements of Molecular Biology and Experiment for modern graduate students,with the central goal of reforming the educational model of higher education institutions and enhancing the effectiveness and quality of talent development.This strategy is designed to nurture highly skilled professionals who can address the current needs of the industry.
基金Humanities and Social Sciences Planning Fund Project of the Ministry of Education of P.R.China,titled“Research on the Books Publishing of Modern Chinese Libraries from the Perspective of Generalized Technology”(Project No.19YJA870014).
文摘The paper is based on literature search,introducing and analyzing the book publishing situation of modern Peking University Library from the perspective of the publisher being Peking University Library and its predecessor.It is pointed out that the majority of the book printing methods published by the modern Peking University Library are lead printing,and among the types of Chinese library classification,catalogue of library collections is the most common.
文摘Building occupants are not immune to ill-health as a result of time they spend in a building.This paper seeks to examine the effects furniture ergonomics have on student’s satisfaction in the library of Universiti Teknologi Malaysia.A pilot survey was initially conducted in the library through a one-to-one interaction with students to fetch their opinions on the general effects of the furniture.An observation through several walkthroughs was also conducted by the researchers to compare and validate responses obtained.Two hundred and sixty-five students that come from fifteen nationalities are surveyed.A structured questionnaire is used to collect data on the respondents’opinions on the size,shape,arrangement and comfort of the furniture.Eta cross tabulation,Spearman’s rho and Kendall’s Tau-b are used to establish relationships.Results show that amongst the effects studied,there are significant positive relationships between students’satisfaction of furniture ergonomics as against back-strain and lack of concentration.This implies that the more the furniture arrangements,size and shape are perceived unsatisfactory,the more their effects on back-strain and lack of concentration towards the students.This paper further recommends that library management should see to designing IEQ(Indoor Environmental Quality)guidelines that will mitigate the effects of furniture ergonomics thus improving student’s satisfaction.
文摘The library seating management system is an important part of the library’s modern services. The system is based on the Spring and Spring Boot framework, and the system realizes a series of functions such as real-time query, reservation, and cancellation of seat resources, providing users with great convenience. With a simple operation, users can know the availability of seats in the library in real time and reserve them according to their needs. At the same time, the system also provides rich management functions, enabling administrators to easily configure and manage seat resources. The addition, deletion, modification and review of users, the generation of seats, the viewing of user usage records, and the addition or deletion of points for users’ usage can also be carried out. This not only improves the management efficiency, but also provides more scientific and accurate data support for the management of the library. The system not only optimizes the user experience, but also promotes the scientific management and efficient utilization of library resources, and provides strong support for the modern services of the library.
文摘In the contemporary digital age,traditional reading habits have been compromised by digital media,a situation that has forced academic libraries to adopt proactive marketing approaches to promote reading among students and faculty.This article investigates the implementation of marketing strategies in promoting reading activities within academic libraries,taking the University of Shanghai for Science and Technology as a case study.The paper explores various marketing strategies employed by academic libraries,including market segmentation,utilization of new media platforms,and the creation of personalized content for readers.Drawing from an extensive literature review and real-world examples,the study establishes a framework for marketing reading promotion activities tailored to academic libraries.These findings underscore how effective marketing strategies can enhance user participation,expand library influence,and optimize resource utilization.
基金The Chongqing University of Engineering for 2024 Construction Project Landscape for Comprehensive Project Library:Riverfront Space Renewal Project of Diaoyuzui Peninsula,Dadukou,Chongqing(Project No.2024-JZ-XMK054-XM083)。
文摘To enhance our talent cultivation model through“school-enterprise cooperation and industry-teaching fusion”,we aim to improve the“4321”Industry-Education Integration System.This includes actively promoting the use of case banks and project banks in teaching to develop students’practical engineering skills through hands-on application of professional knowledge.Additionally,landscape design courses emphasize practical learning experiences to implement the fundamental goal of“cultivating morality”.Guided by enhancing students’practical skills,we ensure alignment with course objectives and professional training requirements,emphasizing the seamless integration of theory and practice.
文摘University libraries,as an integral component of university research capabilities,are tasked with serving education by providing research support to faculty and students.This study adopts an affordance research perspective originating from ecological psychology to explore the optimization path of research support services in libraries in the new media era.Based on the characteristics of university library services,this research analyzes three aspects:production affordance,mobile affordance,and social affordance,and proposes practical recommendations to guide future research service practices of university libraries.
基金part of the research results of Humanities and Social Science Planning Fund Project of the Ministry of Education of P.R.China,titled“Research on the Books Publishing of Modern Chinese Library From the Perspective of Generalized Technology”(Project No.19YJA870014).
文摘Based on several bibliographic advertisements carried in newspapers and magazines from 1919 to 1922 for books published by Peking University Publishing Department,and issued by Yadong Library,the paper analyzes the characteristics of the books published by Peking University Publishing Department during this period,and points out that Yadong Library entered its golden period of development due to move from the alley to the main road and obtain the management rights of the books published by Peking University Publishing Department.
文摘Since the age of digital intelligence,the government has introduced policies to actively promote the intelligent transformation of grassroots public cultural services.Based on the investigation and analysis of the development status quo of grassroots libraries in Henan Province,we put forward the path of high-quality development of grassroots libraries in Henan,namely,improving the mechanism and system,leading with digital intelligence technology,empowering by Yellow River culture(the heritage and values rooted in the history and traditions of the Yellow River region),and driven by users’demand.