Interaction of major facilitator superfamily domain containing 2A with the blood-brain barrier

The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the blood-brain barrier remain poorly understood.The major facilitator superfamily domain containing 2A has been identified as a key regulator of blood-brain barrier function.It plays a critical role in promoting and maintaining the formation and functional stability of the blood-brain barrier,in addition to the transport of lipids,such as docosahexaenoic acid,across the blood-brain barrier.Furthermore,an increasing number of studies have suggested that major facilitator superfamily domain containing 2A is involved in the molecular mechanisms of blood-brain barrier dysfunction in a variety of neurological diseases;however,little is known regarding the mechanisms by which major facilitator superfamily domain containing 2A affects the blood-brain barrier.This paper provides a comprehensive and systematic review of the close relationship between major facilitator superfamily domain containing 2A proteins and the blood-brain barrier,including their basic structures and functions,cross-linking between major facilitator superfamily domain containing 2A and the blood-brain barrier,and the in-depth studies on lipid transport and the regulation of blood-brain barrier permeability.This comprehensive systematic review contributes to an in-depth understanding of the important role of major facilitator superfamily domain containing 2A proteins in maintaining the structure and function of the blood-brain barrier and the research progress to date.This will not only help to elucidate the pathogenesis of neurological diseases,improve the accuracy of laboratory diagnosis,and optimize clinical treatment strategies,but it may also play an important role in prognostic monitoring.In addition,the effects of major facilitator superfamily domain containing 2A on blood-brain barrier leakage in various diseases and the research progress on cross-blood-brain barrier drug delivery are summarized.This review may contribute to the development of new approaches for the treatment of neurological diseases.

Phase structure evolution and coercivity mechanism of high-Co containing permanent magnets

The phase structure and magnetic properties of high-Co containing permanent magnets with high thermal stability have been systematically studied in this work.It is abnormal that the coercivity of annealed samples was slightly lower than that of sintered samples,while the coercivity was usually enhanced after annealing in conventional Nd–Fe–B samples.Further analysis showed that in addition to RE2(Fe,Co)14B main phase and RE-rich grain boundary phase,there were also new Co-rich magnetic phases located in the grain boundary.During annealing,the phase structures of high-Co containing magnets were readjusted,especially the increasing Co-rich magnetic phase and emerging RE-rich particles precipitated from the main phase.Eventually,the isolated RE-rich particles would act as the pinning center of the domain wall movement in demagnetization process.It was confirmed that the coercivity of annealed high-Co containing magnets was controlled by both nucleation and pinning.Pinning mechanism can partially compensate for the weakening of magnetic isolation due to increased Co-rich magnetic phase,which explained the moderate decrease in coercivity of annealed high-Co containing magnets.The discovery of new coercivity mechanism contributed to in-depth understanding of high-Co containing magnets.

Effect of forging on the microstructure and texture of a high Nb containing γ-TiAl alloy

The effect of forging on the microstructure and texture evolution of a high Nb containing Ti-45Al-7Nb-0.3W(at.%)alloy was investigated by X-ray diffractometer(XRD),scanning electron microscopy(SEM),and transmission electron microscopy(TEM).The results show that the as-cast alloy is mainly composed of α_(2)/γ lamellar colonies with a mean size of 70μm,but the hot-forged pancake displays a near duplex microstructure(DP).Kinking and bending of lamellar colonies,deformation twinning and dynamic recrystallization(DRX)occur during hot forging.Meanwhile,dense dislocations in theβphase after forging suggest that the high-temperature β phase with a disordered structure is favorable for improving the hot-workability of the alloy.Unlike the common TiAl casting texture,the solidification process of the investigated as-cast alloy with high Nb content is completely via the β phase region,resulting in the formation of a<110>γ fiber texture where the<110>γ aligns parallel to the heat-flow direction.In comparison,the relatively strong<001>and weak<302>texture components in the as-forged alloy are attributed to the deformation twinning.After annealing,static recrystallization occurs at the twin boundary and intersections,which weakens the deformation texture.

High patatin like phospholipase domain containing 8 expression as a biomarker for poor prognosis of colorectal cancer

BACKGROUND Patatin like phospholipase domain containing 8(PNPLA8)has been shown to play a significant role in various cancer entities.Previous studies have focused on its roles as an antioxidant and in lipid peroxidation.However,the role of PNPLA8 in colorectal cancer(CRC)progression is unclear.AIM To explore the prognostic effects of PNPLA8 expression in CRC.METHODS A retrospective cohort containing 751 consecutive CRC patients was enrolled.PNPLA8 expression in tumor samples was evaluated by immunohistochemistry staining and semi-quantitated with immunoreactive scores.CRC patients were divided into high and low PNPLA8 expression groups based on the cut-off va-lues,which were calculated by X-tile software.The prognostic value of PNPLA8 was identified using univariate and multivariate Cox regression analysis.The over-all survival(OS)rates of CRC patients in the study cohort were compared with Kaplan-Meier analysis and Log-rank test.RESULTS PNPLA8 expression was significantly associated with distant metastases in our cohort(P=0.048).CRC patients with high PNPLA8 expression indicated poor OS(median OS=35.3,P=0.005).CRC patients with a higher PNPLA8 expression at either stage I and II or stage III and IV had statistically significant shorter OS.For patients with left-sided colon and rectal cancer,the survival curves of two PN-PLA8-expression groups showed statistically significant differences.Multivariate analysis also confirmed that high PNPLA8 expression was an independent prog-nostic factor for overall survival(hazard ratio HR=1.328,95%CI:1.016-1.734,P=0.038).

GATA binding protein 2 mediated ankyrin repeat domain containing 26 high expression in myeloid-derived cell lines

BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ANKRD26.However,the positive regulators of ANKRD26 are still unknown.AIM To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 transcription.METHODS Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family POTE.According to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activity of the mitogen activated protein kinase and extracellular signal regulated kinase 1/2 signaling pathways,which are potentially involved in the regulation of thrombopoietin-dependent signaling and further impair proplatelet formation by megakaryocytes(MKs)[11].However,the positive regulators of ANKRD26,which might be associated with THC2 pathology,are still unknown.

Application of Thermodynamic Database to Corrosion of ZrO_(2) Containing Submerged Entry Nozzle in Steel Continuous Casting Process

The CALPHAD thermodynamic databases are very useful to analyze the complex chemical reactions happening in high temperature material process.The FactSage thermodynamic database can be used to calculate complex phase diagrams and equilibrium phases involving refractories in industrial process.In this study,the FactSage thermodynamic database relevant to ZrO_(2)-based refractories was reviewed and the application of the database to understanding the corrosion of continuous casting nozzle refractories in steelmaking was presented.

Purification and Characteristics of Mn-containing Nitrogenase Component Ⅰ

A mutant UW 3, which is unable to fix N 2 in the presence of Mo (Nif -) but undergo phenotypic reversal to Nif + under Mo deficiency, was able to grow in Mo- and NH 3-deficient medium containing Mn, and the growth was accelerated by Mn at low concentration. A partly purified nitrogenase component Ⅰ protein separated from UW 3 grown in the Mn-containing medium was shown to contain Fe and Mn atoms (ratio of Fe/Mo/Mn: 10.41/0.19/1.00) with C 2H 2- and H +-reducing activity which almost equal to half of that of MoFe protein purified from wild-type mutant of Azotobacter vinelandii Lipmann. This protein was obviously different from MoFe protein in both absorption spectrum and circular dichroism, and the molecular weight of subunits in Mn-containing protein was close to that of α subunit in MoFe protein. The preliminary results indicated that the protein containing Mn might be a nitrogenase component Ⅰ protein.

Preparation and characterization of manganese - containing silicates with Z S M - 48 structure

报道了新型杂原子分子筛Ｍｎ－ＺＳＭ－４８的合成与表征。红外光谱和紫外漫反射光谱测试表明Ｍｎ（Ⅱ）离子进入了分子筛骨架。Ｍｎ（Ⅱ）同晶取代Ｓｉ（Ⅳ）导致产物晶粒的形貌和热性质有所不同。反应混合物中１，６－己二胺的含量，水含量和ＳｉＭｎ比对产物的晶化有很大影响。

Mechanical property and permeability of raw coal containing methane under unloading confining pressure

Based on domestic-developed triaxial servo-controlled seepage equipment for thermal-hydrologicalmechanical coupling of coal containing methane,an experimental study was carried out to investigate mechanical property and gas permeability of raw coal,under the situation of conventional triaxial compression and unloading confining pressure tests in different gas pressure conditions.Triaxial unloading confining pressure process was reducing confining pressure while increasing axial pressure.The research results show that,compared with the peak intensity of conventional triaxial loading,the ultimate strength of coal samples of triaxial unloading confining pressure was lower,deformation under loading was far less than unloading,dilation caused by unloading was more obvious than loading.The change trend of volumetric strain would embody change of gas permeability of coal,the permeability first reduced along with volumetric strain increase,and then raised with volume strain decrease,furthermore,the change trends of permeability of coal before and after destruction were different in the stage of decreasing volume strain due to the effect of gas pressure.When gas pressure was greater,the effective confining pressure was smaller,and the radial deformation produced by unloading was greater.When the unloading failed confining pressure difference was smaller,coal would be easier to get unstable failure.

Effects of Rare Earths and Al on Structure and Performance of High Chromium Cast Iron Containing Wolfram

Effects of RE and Al on the structure, impact toughness, hardness, and wear resistance of high chromium cast iron containing wolfram were investigated. The results show that without modification the volume fraction of austenite is high and the carbide appears to be thick lath and the grain size is relatively large; proper modification using RE combined with Al can reduce volume fraction of residual austenite in the as-cast structure obviously, refine grain size of primary austenite notably, and make the morphology of carbide changing from thick lath to thin lath, rosette, and feather-like modification can also increase hardness, wear resistance and impact toughness of cast iron.

Effect of CaO and CaCO3 on Reduction Rate of Iron Ore Pellets Containing Carbon

The effect of metallurgical fluxes CaO and CaCO3 on the reduction rate of iron ore pellets containing carbon in nitrogen atmosphere has been studied by a weight-loss thermal balance. The experimental results showed that adding CaO or CaCO3 can promote reduction reaction as the added CaO or CaCO3 probably decrease the apparent activation energy of iron ore concentrate-carbon-CaO or CaCO3 reaction, and the reduction rate constant changes with mass percent of CaO and CaCO3. The kinetic analysis also showed that the rate-controlling step of the reaction is inner gas diffusion.

Giant exophytic cystic adenomyosis with a levonorgestrel containing intrauterine device out of the uterine cavity after uterine myomectomy: A case report

BACKGROUND A cystic lesion arising from the myometrium of the uterus, termed as cystic adenomyosis, has chocolate-like, thick viscous contents and contains various amounts of endometrial stroma below the glandular epithelium. It is an extremely rare type of adenomyosis.CASE SUMMARY Herein, we report an unusual case of a giant cystic mass in the pelvic cavity after uterine myomectomy. The patient complained of abnormal uterine bleeding and severe dysmenorrhea. After a levonorgestrel-containing intrauterine device(LNG-IUD) was inserted, her symptoms were greatly alleviated. However, the LNG-IUD was detected in the cystic cavity during the follow-up. For fear of the intrauterine device migrating into and damaging the surrounding viscera,surgical treatment was proposed. Therefore, laparoscopic resection of the lesion and removal of the LNG-IUD were performed and cystic adenomyosis with an LNG-IUD out of the uterine cavity was diagnosed.CONCLUSION We believe that myomectomy breaking through the endometrial cavity may have been a predisposing factor for the development of cystic adenomyosis in this case.

Development and validation of THM coupling model of methane-containing coal

Based on nine necessary basic assumptions for THM coupling model,this research comprehensively applied the theories of elastic mechanics,seepage mechanics and heat transfer,and established a real three-field and two-way coupled mathematical model to reveal the connections among seepage field,deformation field and temperature field within the system of methane-containing coal.In comparison between numerical and analytical solutions,the coupling modeling for THM of methane-containing coal was proved to be correct by model application in the physical simulation experiment of coal and gas outburst.The model established in this paper was the improvement of traditional seepage theory of methane-containing coal and fluid-solid coupled model theory,which can be widely used in prevention of coal and gas outburst as well as exploitation of coal bed methane.

Effects of Fermentation Product Containing Phytase on Productive Performance,Egg Quality,and Phosphorous Apparent Metabolism of Laying Hens Fed Different Levels of Phosphorus

This study investigated the effects of fermentation product containing phytase （FPP） that was fermented using waste vinegar residue （WVR） as substrate from Aspergillusficuum NTG-23 on productive performance, egg quality, and phosphorus apparent metabolism of laying hens. First, 375 22-wk-old Jinghong hens were allocated into 5 treatments （5 replicates of 15 hens each） in an 8-wk experiment for evaluating the parameters of productive performance, egg quality, serum, and tibia. Experimental diets contained 4% FPP and 96% corn-soybean diet. The levels of dicalciurn phosphate （DCP） were 1.34, 1.01, 0.67, 0.34 and 0%. Next, thirty 31-wk-old Jinghong hens were fed 5 types of diets for evaluating phosphorous apparent metabolism rate. Egg productive rate, egg weight, feed conversion ratio, Haugh unit, egg albumen height, serum calcium, tibia ash, tibia ash calcium and tibia breaking strength were not different significantly among 5 treatments. The significant difference of average daily feed intake was not appeared when the DCP content of corn-soybean-FPP diet was reduced to 0.67%; the eggshell hardness, eggshell thickness and serum phosphorus were not reduced significantly until the DCP content of corn-soybean-FPP diet was reduced to 0.34%. The yolk color was improved when the laying hens fed deficient DCP corn-soybean-FPP diet. A 22.14% reduction in excreta phosphorus was observed when the laying hens fed low phosphorus （0.67% DCP） corn-soybean-FPP diet. A 30% elevation of phosphorus apparent metabolism rate was obtained when the DCP content of corn-soybean-FPP diet was decreased from 1.34 to 1.01%. The reducing cost of layer diet was totalized about 120 CNY 1000 kg1 diet when the content of DCP was 0.67% in corn-soybean-FPP diet. These results indicated that FPP could be applied in laying hen as a potential, cost-effective and rational application of WVR.

Synthesis and Bacteriostatic Activity and Antifouling Capability of Benzamide Derivatives Containing Capsaicin

Five benzamide deriatives containing capsaicin were synthesized which have similar structures to capsaicin. Their yield was high. The monomers synthesized were characterized by IR, 1H NMR and MS spectroscopy. Cha-racterization data are in agreement with the proposed structures of the products. These five compounds exhibit bacterial inhibition and N-[4-hydroxy-2-methyl-5-（methylthio）benzyl]benzamide（HMMBBA）, for instance, shows that the minimal inhibitory concentrations（MIC） of HMMBBA are 0.125 and 0.25 mg/mL on Staphyloccocus aureus and Escherichia coli, respectively. A static test site was set up in the eighth harbor to investigate the antifouling effectiveness of the five new antifoulants. Five-month exposure experiments were performed on sets of panels coated with each of antifouling coatings, and the results were compared to that of the test panel without antifouling coating. Test boards with antifouling coating were covered with just a macroscopic fouling organism such as Balanus. The results of the present paper demonstrate that new antifoulants represent an alternative to the biocidal antifouling paint.

STUDY ON SYNTHESES AND PROPERTIES OF SOME NOVEL POLYIMIDES CONTAINING BISTHIAZOLE RINGS

Some novel polyimides containing bisthiazole rings were prepared by reacting 2,2'-diamino-4, 4'-bisthiazole (DART) with different aromatic dianhydride. The polyimides obtained had inherent viscosities of 0.37-0.82 dl/g. Thermogravimetric analysis of the polyimides showed good thermal stability, the temperature at 5% weight loss being from 450 degrees to 560 degrees C. The permeability of two polymer membranes to H-2, O-2 and N-2 was determined, respectively. Three kinds of polyimide films were converted into electrical conductor by pyrolysis at high temperature in nitrogen atmosphere. The maximum room temperature conductivity as high as 3.9x10(2) S/cm for PI him pyrolyzed at 1200 degrees C for 10 min was obtained, and it was very stable in air.

Scattering of plane P waves by canyons containing multiple circular-arc-shaped layers

By expressing the wave functions in the form of Fourier-Bessel series, the analytical solution for the two-dimension scattering problem of plane P waves by the cylindrical canyon topography that contains arbitrary number of circular-arc-shaped layers is presented firstly. And then, the convergence of the proposed series solution with the truncation number of terms is discussed, which demonstrates that the analytical solution can converge even for very high frequencies of the incident P wave. Finally, using this solution, the influences that are imposed on the stationary ground motion by the number and the sequence of alluvial layers, as well as the stiffness of soft interlayer contained in the canyon, are studied,

Multi-Effect Evaporation Coupled with MVR Heat Pump Thermal Integration Distillation for Separating Salt Containing Methanol Wastewater

Due to the high energy consumption for separation of salt containing methanol wastewater, in this work, the multi-effect evaporation coupled with mechanical vapor recompression (MVR) heat pump and thermal integration technologies were raised for the first time. The ELECNRTL thermodynamic model is used to simulate and optimize the evaporation rectification process. Energy consumption and total annual cost (TAC) are taken as objective functions. The results show that multi-effect evaporation coupled with conventional distillation process can save energy consumption and TAC by 44.12% and 39.14%. The multi-effect evaporation coupled with distillation process based on MVR heat pump technology can save energy consumption and TAC by 55.27% and 47.49%, which is super to three-effect evaporation coupled with conventional distillation process. The three-effect evaporation coupled with MVR heat integration process can save energy consumption and TAC by 81.32% and 58.55%, which is more economical than other processes. It can be clearly seen that three-effect evaporation coupled with MVR heat integration process is more competitive to deal with the salt containing methanol wastewater.

Electrical Investigation of Some Silver Containing Ceramics Obtained from Coprecipitated Oxalates

Silver-ceramics (Ag2MO2)(M=Co, Ni, or Cu) were prepared through thermal decompositionof coprecipitated oxalates. Pellets of these materials were annealed at 350℃ for different timeperiods up to 5 h. The effect of annealing time on the transport properties was studied. IR andX-ray spectra were also studied.

Determination of Organochlorine and Nitrogen Containing Pesticide Residues in Water,Sediments and Fish Samples by Reverse Phase High Performance Liquid Chromatograph

With high performance liquid chromatography, the concentrations of α-endosulfan, DDE, parathion methyl, isoproturon, atrazine, carbaryl and carbofuran were determined in farmed raised fish Labeo rohita of two weight groups. All these pesticide residues were detected in fish meat samples. All these pesticide residues except isoproturon were identified in soil sediments, whereas all these pesticide residues except isoproturon, carbaryl and DDE were also present in water samples. DDT, heptachlor, β-endosulfan, chlorpyrifos, dimethoate, captan, cypermethrin, chlorobromuron and chlorotoluron were absent in all samples of water, sediments and fish flesh. The pesticide residues levels were in order of parathion methyl 〉 DDE 〉 carbofuran 〉 atrazine 〉 α-endosulfan 〉 isoproturan 〉 carbaryl. The concentrations of pesticides were higher in fish weighing 800 -1 300 g than in those weighing 250 -750 g. The DDE was remained highest in sediments, while that was not detected in water samples. The pesticides （endosulfan, parathion methyl, atrazine and carbofuran） had crossed their MRL values （0.001 μg/g） in water samples.