KL-SG High-Speed Rail-a catalyst for national economic development Sri Viknesh Permalu and Karthigesu Nagarajoo

Purpose-In an increasingly interconnected world,transportation infrastructure has emerged as a critical determinant of economic growth and global competitiveness.High-speed rail(HSR),characterized by its exceptional speed and efficiency,has garnered widespread attention as a transformative mode of transportation that transcends borders and fosters economic development.The Kuala Lumpur-Singapore(KL-SG)HSR project stands as a prominent exemplar of this paradigm,symbolizing the potential of HSR to serve as a catalyst for national economic advancement.Design/methodologylapproach-This paper is prepared to provide an insight into the benefits and advantages of HSR based on proven case studies and references from global HSRs,including China,Spain,France and Japan.Findings-The findings that have been obtained focus on enhanced connectivity and accessibility,attracting foreign direct investment,revitalizing regional economies,urban development and city regeneration,boosting tourism and cultural exchange,human capital development,regionai integration and environmental and sustainability benefits.Originality/value-The KL-SG HSR,linking Kuala Lumpur and Singapore,epitomizes the potential for HSR to be a transformative agent in the realm of economic development.This project encapsulates the aspirations of two dynamic Southeast Asian economies,united in their pursuit of sustainable growth,enhanced connectivity and global competitiveness.By scrutinizing the KLSG High-Speed Rail through the lens of economic benchmarking,a deeper understanding emerges of how such projects can drive progress in areas such as cross-border trade,tourism,urban development and technological innovation.

Comparative proteomics analysis reveals the domesticated Lepista sordida primordium differentiation regulation mechanism and the subsequent different development patterns in the pileus and stipe

The wild Lepista sordida is a kind of precious and rare edible fungus.An excellent strain of it by artificial domestication was obtained,which was high-yield and high in iron content.In this study,high-throughput comparative proteomics was used to reveal the regulatory mechanism of its primordium differentiation in the early fruiting body formation.The mycelium before the primordium differentiation mainly expressed high levels of mitochondrial functional proteins and carbon dioxide concentration regulatory proteins.In young mushrooms,the highly expressed proteins were mainly involved in cell component generation,cell proliferation,nitrogen compound metabolism,nucleotide metabolism,glutathione metabolism,and purine metabolism.The differential regulation patterns of pileus and stipe growth to maturity were also revealed.The highly expressed proteins related to transcription,RNA splicing,the production of various organelles,DNA conformational change,nucleosome organization,protein processing,maturation and transport,and cell detoxification regulated the pileus development and maturity.The proteins related to carbohydrate and energy metabolism,large amounts of obsolete cytoplasmic parts,nutrient deprivation,and external stimuli regulated the stipe development and maturity.Multiple CAZymes regulated nutrient absorption,morphogenesis,spore production,stress response,and other life activities at different growth and development stages.

The environment,especially the minimum temperature,affects summer maize grain yield by regulating ear differentiation and grain development

Ear differentiation,grain development and their interaction with factors in the growing environment,such as temperature,solar radiation and precipitation,greatly influence grain number and grain weight,and ultimately affect summer maize production.In this study,field experiments involving different sowing dates were conducted over three years to evaluate the effects of temperature factors,average solar radiation and total precipitation on the growth process,ear differentiation,fertilization characteristics,grain filling and yield of summer maize varieties with different growth durations.Four hybrids were evaluated in Huang-Huai-Hai Plain(HHHP),China from 2018 to 2020 with five different sowing dates.The results showed that the grain yield formation of summer maize was strongly impacted by the environment from the silking(R1)to milking(R3)stage.Average minimum temperature(AT_(min))was the key environmental factor that determined yield.Reductions in the length of the growing season(r=–0.556,P<0.01)and the total floret number on ear(R^(2)=0.200,P<0.001)were found when AT_(min) was elevated from the emerging(VE)to R1 stage.Both grain-filling rate(R^(2)=0.520,P<0.001)and the floret abortion rate on ear(R^(2)=0.437,P<0.001)showed quadratic relationships with AT_(min) from the R1 to physiological maturity(R6)stage,while the number of days after the R1 stage(r=–0.756,P<0.01)was negatively correlated with AT_(min).An increase in AT_(min) was beneficial for the promotion of yield when it did not exceeded a certain level(above 23°C during the R1–R3 stage and 20–21°C during the R1-R6 stage).Enhanced solar radiation and precipitation during R1–R6 increased the grain-filling rate(R^(2)=0.562,P<0.001 and R^(2)=0.229,P<0.05,respectively).Compared with short-season hybrids,full-season hybrids showed much greater suitability for a critical environment.The coordinated regulation of AT_(min),ear differentiation and grain development at the pre-and post-silking stages improved maize yield by increasing total floret number and grain-filling rate,and by reducing the floret abortion rate on ear.

Differentiated Paths of Regional Green Development Driven by Industrial Enterprise Innovation

A fundamental shift in the regional development pattern is crucial to achieving a comprehensive green transformation in China.Currently,innovation-driven green development is a significant strategic option for regional development.As the main body of innovation and the basic unit of regional composition,enterprises have a profound impact on the development of regional economy,society,ecology,and other aspects.However,considering China’s vast territory and significant regional differences in natural environment and industrial structure,it’s necessary to further explore the specific impact paths of regional green development driven by enterprise innovation.Therefore,taking industrial enterprises as an example,based on the panel data of 30 provinces in China from 2016 to 2020,this study verifies the impact of industrial enterprise innovation on the regional green development level by constructing a parallel multiple mediating effect model and dividing the economy into eastern,central,and western regions to discuss the specific impact paths.The results show that industrial enterprise innovation has a significant positive effect on regional green development level,via different influencing paths in each region:(1)The eastern region improves the regional green development level by narrowing the urban-rural income gap;(2)The central region improves the regional green development level by reducing resource dependence;and(3)The western region raises the regional green development level by improving the rationalization of industrial structure.

Power supply arm protection scheme of high-speed railway based on wide-area current differential

When fault occurs on cross-coupling autotransformer(AT)power supply traction network,the up-line and down-line feeder circuit breakers in the traction substation trip at the same time without selectivity,which leads to an extended power failure.Based on equivalent circuit and Kirchhoff’s current law,the feeder current characteristic in the substation,AT station and sectioning post when T-R fault,F-R fault,and T-F fault occur are analyzed and their expressions are obtained.When the traction power supply system is equipped with wide-area protection measurement and control system,the feeder protection device in each station collects the feeder currents in other two stations through the wide-area protection channel and a wide-area current differential protection scheme based on the feeder current characteristic is proposed.When a short-circuit fault occurs in the power supply arm,all the feeder protection devices in each station receive the feeder currents with time stamp in other two stations.After data synchronous processing and logic judgment,the fault line of the power supply arm can be identified and isolated quickly.The simulation result based on MATLAB/Simulink shows that the power supply arm protection scheme based on wide-area current differential has good fault discrimination ability under different fault positions,transition resistances,and fault types.The verification of measured data shows that the novel protection scheme will not be affected by the special working conditions of the electrical multiple unit(EMU),and reliability,selectivity,and rapidity of relay protection are all improved.

Current status and reflection on the development of high-speed maglev transportation

Purpose–High-speed maglev technology can address the issues of adhesion,friction,vibration and highspeed current collection in traditional wheel-rail systems,making it an important direction for the future development of high-speed rail technology.Design/methodology/approach–This paper elaborates on the demand and significance of developing high-speed maglev technology worldwide and examines the current status and technological maturity of several major high-speed maglev systems globally.Findings–This paper summarizes the challenges in the development of high-speed maglev railways in China.Based on this analysis,it puts forward considerations for future research on high-speed maglev railways.Originality/value–This paper describes the development status and technical maturity of several major high-speed maglev systems in the world for the first time,summarizes the existing problems in the development of China’s high-speed maglev railway and on this basis,puts forward the thinking of the next research of China’s high-speed maglev railway.

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

Application of mRNA Differential Display to the Identification of Genes Related to Embryonic Development

mRNA differential display was established by Liang and Pardee in 1992 for the purpose of displaying the mRNA differences between two tissues. The early embryonic development in animals is primarily controlled by the maternal RNAs stored in egg. These mRNAs are being degraded as the development proceeds. In some animals, such as fish and amphibian, new transcripts do not appear until the midblastula stage (midblastula transition, MBT). In other animals, for example in mouse, the zygotic genes are expressed during very early stages of development. The mRNA programmed synthesis and degradation during embryonic development controls the cell differentiation, germlayer formation and pattern formation. All these mRNA changes could be displayed side by side as cDNA band differences by mRNA differential display and the genes corresponding to these differential mRNAs could thus be obtained.

Transcriptome analysis reveals key differentially expressed genes involved in wheat grain development

Wheat seed development is an important physiological process of seed maturation and directly affects wheat yield and quality. In this study, we performed dynamic transcriptome microarray analysis of an elite Chinese bread wheat cultivar(Jimai 20) during grain development using the Gene Chip Wheat Genome Array. Grain morphology and scanning electron microscope observations showed that the period of 11–15 days post-anthesis(DPA) was a key stage for the synthesis and accumulation of seed starch. Genome-wide transcriptional profiling and significance analysis of microarrays revealed that the period from 11 to 15 DPA was more important than the 15–20 DPA stage for the synthesis and accumulation of nutritive reserves.Series test of cluster analysis of differential genes revealed five statistically significant gene expression profiles. Gene ontology annotation and enrichment analysis gave further information about differentially expressed genes, and Map Man analysis revealed expression changes within functional groups during seed development. Metabolic pathway network analysis showed that major and minor metabolic pathways regulate one another to ensure regular seed development and nutritive reserve accumulation. We performed gene co-expression network analysis to identify genes that play vital roles in seed development and identified several key genes involved in important metabolic pathways. The transcriptional expression of eight key genes involved in starch and protein synthesis and stress defense was further validated by q RT-PCR. Our results provide new insight into the molecular mechanisms of wheat seed development and the determinants of yield and quality.

Identification of Differentially Expressed Genes Associated with Cotton Fiber Development in a Chromosomal Substitution Line(CS-B22sh)

One of the impediments in the genetic improvement of cotton fiber is the paucity of information about genes associated with fiber development.Availability of chromosome arm substitution line CS-

Some Critical Issues in the Development of Chinese High-Speed Rail: Challenges and Coping Strategies

In this paper, several critical issues related to Chinese high-speed rails (CHSR) are analyzed, investigated and discussed, including development background, reasons for high ticket fare, some typical factors that influence the environmental life-cycle assessment, time and cost comparison of typical door-to-door travel routes between different transport modes and complementary strategies among different transport modes. First, the results show that the expanding high-speed rail network increased the pressure on the country's roads in the 2010 spring festival rush period due to the high ticket fare of CHSR. Second, due to lower than expected ridership because of the high ticket price and limited demand in less developed areas, some Chinese CHSR projects have become economically unsustainable. Third, without sufficient ridership and service life, the utilization of HSR was unable to show any advantages in the environmental impact compared with the other transport modes. In addition, the impact of shock from CHSR on Chinese domestic civil airlines is evident when the travel distance is below 1050 km. The key objective of the investigation is to enhance our understanding of the development and operation of Chinese high-speed rail. One of the key contributions of the current paper is the presented suggestions for complementary strategies between different transport modes to make full use of Chinese transportation resources to promote low carbon economy.

Simulation Analysis Module of High-speed Rail Bearings Based on Secondary Development in ADAMS

This paper develops a strong secondary development based on ADAMS feature which creates high-speed rail bearings for simulation analysis module. This thesis is in the case of non-circular pattern instructions of how to achieve rapid roller modeling, with analysis of functions and parameters required for the design of the simulation module of the high-speed rail bearing, as well as the design of dialog boxes, the environment and file structure. The specific modules is based on the secondary development language provided by ADAMS ! View. Through the menus, dialog boxes which input parameters, it can achieve high iron bearing automatic modeling, dynamic analysis and post-processing to simplify the analysis of high-speed rail bearing operations, as well as improving the high-speed rail bearing development efficiency.

Rural Development Transformation and Social Governance from the Perspective of Specialization:A Case Study of Ruiling Village in Guangzhou City,China

The reorganization of the global production network and the increased mobility of urban-rural elements have accelerated the differentiation of rural spaces,resulting in a trend towards specialization in rural areas.This paper focuses on the concept of specialization,and takes a renowned bonsai specialized village in Guangzhou City,China as a typical case to explore its transformation and development process,action logic and coupling mechanism with the social governance.The results show that:1)the development of rural specialization in Ruiling Village is shaped by several key actors,such as local governments,grassroots organizations,villagers,social organizations,policies,markets and infrastructure.The transfer of these key actors and the reconstruction of actor-networks in different stages have an important influence on the transformation and realization of rural development goals.2)Through subjects-driven,organization management and resource connection,social governance supports the specialization of rural industrial organization,operation,technology and mode.The governance structure shifts from decentralization to networking,refinement and integration.A coupling community of social governance and specialization is created by the social governance system of‘local government-grassroots organizationseconomic cooperatives-villagers’,which links industries,industrial chains and cooperatives.3)Social governance integrates into the value chain extension process of rural specialized development with a central,multi-level organizational structure.It promotes the construction of the whole value chain through organizational planning,resource integration and image shaping,thus realizing the value,branding and organization of the industry,as well as the integration of rural sustainable development and governance mechanism.Social governance has a profound impact on the development of rural specialization in terms of subject,structure and mode,and participates in the construction of the whole value chain of rural areas.This paper provides a new perspective for understanding the coupling effect of social governance in the rural development transformation,and promotes the rural sustainable development and governance.

Comparative Transcriptome Analyses Reveal Genes Related to Spine Development in Cucumber (Cucumis sativus)

Fruit spine is an important quality trait of cucumber.To better understand the molecular basis of cucumber spine development and function,RNA-Seq was performed to identify differentially expressed genes(DEGs)in fruit spines of different development stages,namely,8 days before anthesis(SpBA8),anthesis(SpA)and 8 days after anthesis(SpAA8).Stage-wise comparisons obtained 2,259(SpBA8 vs.SpA),4,551(SpA vs.SpAA8),and 5,290(SpBA8 vs.SpAA8)DEGs.All the DEGs were classified into eight expression clusters by trend analysis.Among these DEGs,in addition to the Mict,Tril,CsTTG1,CsMYB6,NS,and Tu genes that have been reported to regulate fruit spine formation,we found that the CsHDG11,CsSCL8,CsSPL8,CsZFP6 and CsZFP8 may also be involved in spine development in cucumber.Our study provides a theoretical basis for further research on molecular mechanisms of spine development in cucumber.

Neural differentiation and synaptogenesis in retinal development

To investigate the pattern of neural differentiation and synaptogenesis in the mouse retina, immunolabeling, Brd U assay and transmission electron microscopy were used. We show that the neuroblastic cell layer is the germinal zone for neural differentiation and retinal lamination. Ganglion cells differentiated initially at embryonic day 13（E13）, and at E18 horizontal cells appeared in the neuroblastic cell layer. Neural stem cells in the outer neuroblastic cell layer differentiated into photoreceptor cells as early as postnatal day 0（P0）, and neural stem cells in the inner neuroblastic cell layer differentiated into bipolar cells at P7. Synapses in the retina were mainly located in the outer and inner plexiform layers. At P7, synaptophysin immunostaining appeared in presynaptic terminals in the outer and inner plexiform layers with button-like structures. After P14, presynaptic buttons were concentrated in outer and inner plexiform layers with strong staining. These data indicate that neural differentiation and synaptogenesis in the retina play important roles in the formation of retinal neural circuitry. Our study showed that the period before P14, especially between P0 and P14, represents a critical period during retinal development. Mouse eye opening occurs during that period, suggesting that cell differentiation and synaptic formation lead to the attainment of visual function.

High-speed train cooperativecontrol based on fractional-ordersliding mode adaptive algorithm

Purpose–This study aims to propose an adaptive fractional-order sliding mode controller to solve the problem of train speed tracking control and position interval control under disturbance environment in moving block system,so as to improve the tracking efficiency and collision avoidance performance.Design/methodology/approach–The mathematical model of information interaction between trains is established based on algebraic graph theory,so that the train can obtain the state information of adjacent trains,and then realize the distributed cooperative control of each train.In the controller design,the sliding mode control and fractional calculus are combined to avoid the discontinuous switching phenomenon,so as to suppress the chattering of sliding mode control,and a parameter adaptive law is constructed to approximate the time-varying operating resistance coefficient.Findings–The simulation results show that compared with proportional integral derivative(PID)control and ordinary sliding mode control,the control accuracy of the proposed algorithm in terms of speed is,respectively,improved by 25%and 75%.The error frequency and fluctuation range of the proposed algorithm are reduced in the position error control,the error value tends to 0,and the operation trend tends to be consistent.Therefore,the control method can improve the control accuracy of the system and prove that it has strong immunity.Originality/value–The algorithm can reduce the influence of external interference in the actual operating environment,realize efficient and stable tracking of trains,and ensure the safety of train control.

Chinese Childrenswear Brand Development Path Based on Differentiated Positioning

Sorting and collecting the data by questionnaire survey and literature analysis,the childrenswear brand elemental analysis is completed.The development goal of differentiated positioning of Chinese childrenswear brands is decided.Four modules which influence the brand differentiated development were extracted to establish the hierarchical analysis framework for the differentiated positioning development of Chinese childrenswear brand.Judgment matrix diagram is constructed by using the analytic hierarchy process(AHP).According to the data analysis and judgment,the core elements,basic elements and auxiliary elements are identified which support the development of differentiated positioning of Chinese childrenswear brands.The clear development path of differentiated positioning of Chinese childrenswear brands is formed,namely to enhance the core essential factors as the key point,to grasp the elements’spirit exactly in differentiated positioning of brand style,childrenswear color feature,green and security of products quality and retail prices which influence the brand differentiated development.The Chinese existing technical advantages was given full play by providing the basic support for the differentiated development of childrenswear brands.Auxiliary elements were heleped to grow by making auxiliary elements play a greater role.The goal was achieved to improve the market competitiveness by development of differentiated positioning of Chinese childrenswear brands.

Lens Capsule HSPG-Perlecan Regulates Lens Fibre Differentiation during Chick Embryo Development

Lens fibre differentiation is a life-long process related with lens transparency, and is particularly intense during development, being related with an FGF-2 antero-posterior gradient at the equator level as the main growth factor involved which has been related with the basal membrane of the lens anlagen known as “Lens capsule”. However the lens fibre differentiation induced by FGF2 depends, as in other biological systems, on the local bioavailability of FGF-2 regulated by their relationship with extracellular matrix molecules as Heparan Sulphate Proteoglycans. Here, we try to clarify how Perlecan (a heparan sulphate proteoglycan specific from basement membranes) is involved in lens fibre differentiation at earliest stages of eye development. Our results show that Perlecan, is a major component in the lens capsule during the earliest stages of lens development in chick embryos being present during lens plate induction, lens vesicle stage and the onset of lens fibre differentiation. In order to demonstrate a direct involvement of HSPG-Perlecan in lens fibre differentiation, we generate depleted lenses by HSPG-Perlecan synthesis disruption and specific enzymatic digestion. The HSPG-Perlecan depleted lens show a significant delay or abolition in the lens fibre differentiation which remains in an immature cells displaying DNA synthesis in the posterior epithelium and a decrease in FGF2 lens expression. These data support the hypothesis that lens capsule HSPG-Perlecan is a key molecule involved in lens fibre differentiation during development, probably by involvement in FGF-2 biodisponibility.

Spatio-temporal Differentiation and Driving Factors of Industrial Ecology of Restricted Development Zone from Adaptive Perspective:A Case Study of Shandong,China

Based on the adaptive analysis paradigm,this paper constructs an evaluation index system and an evaluation model of the level of industrial ecology of a restricted development zone from the perspective of the industrial system and of the environmental system,and studies the spatial-temporal differentiation characteristics and the driving factors of the level of industrial ecology of the restricted development zone of the Shandong Province,China,by using a variety of measurement methods.The results show that:1)In the temporal dimension,the level of industrial ecology of the research area increased from 2005 to 2017,while in the regional dimension,it was higher in the eastern coastal areas,followed by the northwestern area and the southwestern area;2)In the spatial dimension,from 2005 to 2017 the level of industrial ecology of the research area had a clear spatial dependence,and the regional spatial agglomeration of the restricted development zones with similar industrial ecology levels become increasingly evident;3)On the whole,the industrial ecology level in the study area had a clear spatial differentiation pattern,as it was higher in the north and in the east and lower in the south and in the west.Moreover,its evolution model changed from a‘three-core driven model’to a‘spatial scattered mosaic distribution model’,and then to a‘single-core driven model’;4)Industrial ecology was positively correlated with economic development,foreign investment,science and technology,and negatively correlated with the government role,while industrial structure and environmental regulation failed to pass the statistical significance test.

ON THE DIFFERENTIATION OF THE ECONOMIC DEVELOPMENT OF CHINA’S NORTH,CENTRAL & SOUTH COASTAL REGIONS

Ⅰ. An analysis of the development trend of the economy in the north,middle aud south coastal regions or China since China began to implementreform and open