In this study, to fabricate dual-pore scaffolds with interconnected pores, Non-solvent Induced Phase Separation （NIPS） and Wire-Network Molding （WNM） techniques were combined. First, a mold with uniform slits was ...In this study, to fabricate dual-pore scaffolds with interconnected pores, Non-solvent Induced Phase Separation （NIPS） and Wire-Network Molding （WNM） techniques were combined. First, a mold with uniform slits was prepared, and needles were inserted into the mold. Subsequently, polycaprolactone （PCL） pellets were dissolved in tetrahydrofuran （THF） at a specified ratio. The slurry was mixed using hot plate stirrer at 1200 rpm for 24 hours at 40 ~C. The PCL slurry was subsequently injected into the mold. Thereafter, to exchange the THF （solvent） with the ethanol （non-solvent）, the mold was soaked in an ethanol bath. After removing the mold from the ethanol bath, the needles were removed from the mold. Consequently, dual-pore scaffold with interconnected pores was obtained. The surface morphology of the fabricated scaffolds were observed using Scanning Electron Microscope （SEM）. Moreover, cell culture experiments were performed using the CCK-8 assay, and the characteristics of cells grown on the dual-pore scaffolds were assessed and were compared with the NIPS-based 3D plotting scaffold.展开更多
随着建筑行业的飞速发展,越来越多的型钢悬挑脚手架与铝合金模板得到了应用,因此,传统的圆钢预埋锚环已不能满足绿色施工节材和回收再利用的要求。该文结合工程实践,自主研发了铝模对应型钢悬挑架反拉钢绳锚环(Cantilever anti pull rop...随着建筑行业的飞速发展,越来越多的型钢悬挑脚手架与铝合金模板得到了应用,因此,传统的圆钢预埋锚环已不能满足绿色施工节材和回收再利用的要求。该文结合工程实践,自主研发了铝模对应型钢悬挑架反拉钢绳锚环(Cantilever anti pull rope anchor ring suspended aluminum mold corresponding steel,简称CAA),总结了反拉钢绳锚环施工应用技术,并从反拉钢绳锚环在实际工程中的应用、施工成本、质量、安装拆除等方面作了充分的介绍与分析,验证了文中的反拉钢绳锚环符合绿色施工节材和回收再利用的要求,是值得推广应用的新型施工技术。展开更多
Tissue engineering （TE） is an integrated discipline that involves engineering and natural science in the development of biological materials to replace, repair, and improve the function of diseased or missing tissue...Tissue engineering （TE） is an integrated discipline that involves engineering and natural science in the development of biological materials to replace, repair, and improve the function of diseased or missing tissues. Traditional medical and surgical treatments have been reported to have side effects on patients caused by organ necrosis and tissue loss. However, engineered tissues and organs provide a new way to cure specific diseases. Scaffold fabrication is an important step in the TE process. This paper summarizes and reviews the widely used scaffold fabrication methods, including conventional methods, electrospinning, three-dimensional printing, and a combination of molding techniques. Furthermore, the differences among the properties of tissues, such as pore size and distribution, porosity, structure, and mechanical properties, are elucidated and critically reviewed. Some studies that combine two or more methods are also reviewed. Finally, this paper provides some guidance and suggestions for the future of scaffold fabrication.展开更多
A novel highly porous 3-D poly（e-caprolactone） （PCL） scaffold with micro-channels was fabricated by injection molding and diluent acetic acids leaching technologies. In this study, the chitosan fiber was employed ...A novel highly porous 3-D poly（e-caprolactone） （PCL） scaffold with micro-channels was fabricated by injection molding and diluent acetic acids leaching technologies. In this study, the chitosan fiber was employed to form the microchannel in PCL matrix. The morphology, porosity and mechanical properties of the scaffolds were studied and calculated. It was found that the larger the content of chitosan fiber is, the higher the porosity would be, due to the volumetric expansion of chitosan fiber in PCL matrix during it being leached. In addition, the less the content of chitosan fiber is, the higher the compressive modulus would be.展开更多
文摘In this study, to fabricate dual-pore scaffolds with interconnected pores, Non-solvent Induced Phase Separation （NIPS） and Wire-Network Molding （WNM） techniques were combined. First, a mold with uniform slits was prepared, and needles were inserted into the mold. Subsequently, polycaprolactone （PCL） pellets were dissolved in tetrahydrofuran （THF） at a specified ratio. The slurry was mixed using hot plate stirrer at 1200 rpm for 24 hours at 40 ~C. The PCL slurry was subsequently injected into the mold. Thereafter, to exchange the THF （solvent） with the ethanol （non-solvent）, the mold was soaked in an ethanol bath. After removing the mold from the ethanol bath, the needles were removed from the mold. Consequently, dual-pore scaffold with interconnected pores was obtained. The surface morphology of the fabricated scaffolds were observed using Scanning Electron Microscope （SEM）. Moreover, cell culture experiments were performed using the CCK-8 assay, and the characteristics of cells grown on the dual-pore scaffolds were assessed and were compared with the NIPS-based 3D plotting scaffold.
基金The authors would like to acknowledge the financial support of the Zhejiang Provincial Natural Science Foundation of China （Grant No. LZ18E050002）, the Science Fund for Creative Research Groups of the National Natural Science Foundation of China （Grant No. 51521064）, the National Natural Science Foundation Council of China （Grant Nos. 51475420 and 51635006）, and the Fundamental Research Funds for the Central Universities of China （Grant No. 2017QNA4003 ）.
文摘Tissue engineering （TE） is an integrated discipline that involves engineering and natural science in the development of biological materials to replace, repair, and improve the function of diseased or missing tissues. Traditional medical and surgical treatments have been reported to have side effects on patients caused by organ necrosis and tissue loss. However, engineered tissues and organs provide a new way to cure specific diseases. Scaffold fabrication is an important step in the TE process. This paper summarizes and reviews the widely used scaffold fabrication methods, including conventional methods, electrospinning, three-dimensional printing, and a combination of molding techniques. Furthermore, the differences among the properties of tissues, such as pore size and distribution, porosity, structure, and mechanical properties, are elucidated and critically reviewed. Some studies that combine two or more methods are also reviewed. Finally, this paper provides some guidance and suggestions for the future of scaffold fabrication.
基金financially supported by the China Scholarship Council and the Wisconsin Institute for Discovery(WID),that enabled the authors to perform this research at the University of Wisconsin-Madison,the National Natural Science Foundation of China(No.51303027)the Scientific Research Staring Foundation,Fujian University of Technology,China(No.GY-Z13028)
文摘A novel highly porous 3-D poly（e-caprolactone） （PCL） scaffold with micro-channels was fabricated by injection molding and diluent acetic acids leaching technologies. In this study, the chitosan fiber was employed to form the microchannel in PCL matrix. The morphology, porosity and mechanical properties of the scaffolds were studied and calculated. It was found that the larger the content of chitosan fiber is, the higher the porosity would be, due to the volumetric expansion of chitosan fiber in PCL matrix during it being leached. In addition, the less the content of chitosan fiber is, the higher the compressive modulus would be.
