Mechanosensors are the most important organelles for osteocytes to perceive the changes of surrounding mechanical environment.To evaluate the biomechanical effectiveness of collagen hillock,cell process and primary...Mechanosensors are the most important organelles for osteocytes to perceive the changes of surrounding mechanical environment.To evaluate the biomechanical effectiveness of collagen hillock,cell process and primary·cilium in lacunar-canalicular system(LCS),we developed pressure-electricity-structure interaction models by using the COMSOL Multiphysics software to characterize the deformation of collagen hillocks-and primary cilium-based mechanosensors in osteocyte under fluid flow and electric field stimulation.And mechanical signals(pore pressure,fluid velocity,stress,deformation)were analyzed in LCS.The effects of changes in the elastic modulus of collagen hillocks,the number and location of cell processes,the length and location of primary cilia on the mechanosensitivity and the overall poroelastic responses of osteocytes were studied.These models predict that the presence of primary cilium and collagen hillocks resulted in significant stress amplifications(one and two orders of magnitude larger than osteocyte body)on the osteocyte.The growth of cell process along the long axis could stimulate osteocyte to a higher level than along the short axis.The Mises stress of the basal body of primary cilia near the top of osteocyte is 8 Pa greater than that near the bottom.However,the presence of collagen hillocks and primary cilium does not affect the mechanical signal of the whole osteocyte body.The established model can be used for studying the mechanism of bone mechanotransduction at the multiscale level.展开更多
ADP晶体{100}面族生长的实时与非实时AFM（atomic force microscopy，AFM）研究表明，过饱和度σ处于0．005～0．04，生长温度介于293～313K之间时，晶面上观察到位错生长丘和其它晶体缺陷所形成的生长丘，晶面主要为台阶推进方式生长...ADP晶体{100}面族生长的实时与非实时AFM（atomic force microscopy，AFM）研究表明，过饱和度σ处于0．005～0．04，生长温度介于293～313K之间时，晶面上观察到位错生长丘和其它晶体缺陷所形成的生长丘，晶面主要为台阶推进方式生长；位错生长丘上空洞的出现与位错弹性理论相符；随过饱和度口降低，台阶形貌会发生相应变化；生长温度为298K时，台阶棱边能不小于6．2×10^-7／cm^2。展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.11972242,11632013,11702183)China Postdoctoral Science Foundation(Grant No.2020M680913).
文摘Mechanosensors are the most important organelles for osteocytes to perceive the changes of surrounding mechanical environment.To evaluate the biomechanical effectiveness of collagen hillock,cell process and primary·cilium in lacunar-canalicular system(LCS),we developed pressure-electricity-structure interaction models by using the COMSOL Multiphysics software to characterize the deformation of collagen hillocks-and primary cilium-based mechanosensors in osteocyte under fluid flow and electric field stimulation.And mechanical signals(pore pressure,fluid velocity,stress,deformation)were analyzed in LCS.The effects of changes in the elastic modulus of collagen hillocks,the number and location of cell processes,the length and location of primary cilia on the mechanosensitivity and the overall poroelastic responses of osteocytes were studied.These models predict that the presence of primary cilium and collagen hillocks resulted in significant stress amplifications(one and two orders of magnitude larger than osteocyte body)on the osteocyte.The growth of cell process along the long axis could stimulate osteocyte to a higher level than along the short axis.The Mises stress of the basal body of primary cilia near the top of osteocyte is 8 Pa greater than that near the bottom.However,the presence of collagen hillocks and primary cilium does not affect the mechanical signal of the whole osteocyte body.The established model can be used for studying the mechanism of bone mechanotransduction at the multiscale level.
文摘ADP晶体{100}面族生长的实时与非实时AFM（atomic force microscopy，AFM）研究表明，过饱和度σ处于0．005～0．04，生长温度介于293～313K之间时，晶面上观察到位错生长丘和其它晶体缺陷所形成的生长丘，晶面主要为台阶推进方式生长；位错生长丘上空洞的出现与位错弹性理论相符；随过饱和度口降低，台阶形貌会发生相应变化；生长温度为298K时，台阶棱边能不小于6．2×10^-7／cm^2。
