Maternal alcohol consumption during pregnancy has detrimental effects on fetal central nervous system development. Maternal alcohol consumption prior to and during pregnancy significantly affects cognitive functions i...Maternal alcohol consumption during pregnancy has detrimental effects on fetal central nervous system development. Maternal alcohol consumption prior to and during pregnancy significantly affects cognitive functions in offspring, which may be related to changes in cyclin-dependent kinase 5 because it is associated with modulation of synaptic plasticity and impaired learning and memory, in this study, we examined adult offspring in a materna~ alcohol consumption model in rats. Y-maze test results showed that in utero exposure to alcohol impairs learning and memory capacities. Cyclin-dependent kinase 5 mRNA and protein expressions in the hippocampus of the offspring were significantly elevated, as assayed by quantitative real-time PCR and reverse transcription-PCR, immunofluorescence, and immuno-precipitation. Our experimental findings strongly suggest that altered cydin-dependent kinase 5 may mediate impaired learning and memory in adult rats that were exposed to alcohol by maternal consumption while in utero.展开更多
基金Acknowledgments We thank Dr Boja Emily S （NHLBI, NIH, USA） for expert technical assistance in mass spectrometry analyses, and our colleagues Drs Bin Lu, Zhao-Qiu Wu for helpful comments. This work was supported by a National Basic Research Program grant from the Ministry of Science and Technology of China （G2003CB515401）, National Science Fund for Distinguished Young Scholar from the National Natural Science Foundation of China （30425002） and a fund supported by the ＂100 Talents Project＂ of Chinese Academy of Sciences （J-G Liu）.
基金supported by the National Nature Science Foundation of China,No.81171261a grant from Henan Science Technology Committee of China,No.112300410162Fund for Talents with Innovation in Medical Science and Technology of Henan Province of China,No.3052
文摘Maternal alcohol consumption during pregnancy has detrimental effects on fetal central nervous system development. Maternal alcohol consumption prior to and during pregnancy significantly affects cognitive functions in offspring, which may be related to changes in cyclin-dependent kinase 5 because it is associated with modulation of synaptic plasticity and impaired learning and memory, in this study, we examined adult offspring in a materna~ alcohol consumption model in rats. Y-maze test results showed that in utero exposure to alcohol impairs learning and memory capacities. Cyclin-dependent kinase 5 mRNA and protein expressions in the hippocampus of the offspring were significantly elevated, as assayed by quantitative real-time PCR and reverse transcription-PCR, immunofluorescence, and immuno-precipitation. Our experimental findings strongly suggest that altered cydin-dependent kinase 5 may mediate impaired learning and memory in adult rats that were exposed to alcohol by maternal consumption while in utero.
