Neosartorya hiratsukae对Cu^(2+)的吸附特征

研究利用真菌Neosartorya hiratsukae(M7)的冻干菌体吸附溶液中Cu2+的特征。利用红外光谱分析了菌体的主要官能团,分别从动力学、等温吸附2方面进行试验,并研究了溶液pH值、Cu2+初始浓度、吸附时间对吸附过程的影响。结果表明,菌体富含羟基、羧基等官能团;吸附的最适pH值为5.0;等温吸附过程可以用Langmuir方程进行描述,菌体对Cu2+的最大吸附量为10.03 mg/g;菌体吸附Cu2+的过程进行得很快,30 min内可达到平衡吸附量的78.6%,吸附在3 h内达到平衡,吸附过程可用二级动力学方程描述(R2=0.998)。

南海柳珊瑚来源真菌Aspergillus hiratsukae SCSIO 7S2001的次生代谢产物及活性研究

目的对1株采自南海柳珊瑚来源曲霉属真菌Aspergillus hiratsukae SCSIO 7S2001进行次级代谢产物及活性研究。方法通过条件优化对菌株进行大规模发酵,采用硅胶柱层析、葡聚糖凝胶、半制备高效液相等色谱学方法对其大米发酵产物进行分离纯化,利用核磁共振、质谱等波谱学技术,结合其理化性质及文献数据对比进行化合物的结构鉴定,并对化合物进行初步抗氧化和抗菌活性测试。结果从菌株SCSIO 7S2001中分离鉴定9个单体化合物cristatumin F(1)、neoechinulin B(2)、cyclo(Trp-Ana)(3)、cyclo(D-Trp-L-Pro)(4)、cyclo(D-Pro-D-Phe)(5)、phomoindene A(6)、β-adenosine(7)、E-6-hydroxy-3-(4-hydroxybenzylidene)-benzo[b]furan-2-one(8)、Z-6-hydroxy-3-(4-hydroxybenzylidene)-benzo[b]furan-2-one(9),并对化合物进行抗菌活性、DPPH自由基清除活性以及乙酰胆碱酯酶抑制活性的测定。化合物1~9均为首次从该菌株中分离得到,化合物8和9为首次从海洋真菌中分离得到的新天然产物。所有化合物均无抗菌活性,化合物6表现出显著的DPPH自由基清除活性,其IC50为8.50μmol/L,几乎与阳性对照相当(阳性对照IC50=5.58μmol/L)。化合物1和2表现出微弱的乙酰胆碱酯酶抑制活性,在质量浓度为50μg/mL下,抑制率分别为16.44%和19.75%。

Rethinking the Debate over Motherhood Protection During the Taisho Period

Motherhood protection means that the government provides protective measures to help mothers during pregnancy and childbirth.During Taisho period,there was a heated discussion over motherhood protection among feminists.The leading feminists in this discussion,Hiratsuka Raicho and Yosano Akiko,held totally opposed ideas towards motherhood protection.It is worth noting that the differences in their views are mainly due to their different life experiences.This heated debate lasted for several years,although it did not reach a concrete conclusion,it left a valuable legacy for later generations of feminists.Analyzing the reasons for their different attitudes and exploring the theoretical logic behind their different attitudes can provide lessons for solving the problems of low fertility and motherhood protection in Japan and the world today.To truly protect women's interest and promote gender equality,motherhood protection should focuses more on the plight of women due to childbirth than national interest,making becoming a mother no longer be an obstacle to women's self-achievement and personal independence.