[Objective] This study aimed optimize sequence-related amplified polymor- phism （SRAP）-PCR system for Paphiopedilum hirsutissimum. [Method] Using P. hir- sutissimum leaf as the material, a single-factor test was ado...[Objective] This study aimed optimize sequence-related amplified polymor- phism （SRAP）-PCR system for Paphiopedilum hirsutissimum. [Method] Using P. hir- sutissimum leaf as the material, a single-factor test was adopted to optimize the fac- tors in SRAP-PCR system, including the concentrations of dNTPs, Mg2＋, Taq poly- merase, DNA template and primers. [Result] The optimized SRAP-PCR system con- tained 2.5 μl of 10xPCR buffer, 0.15 mmol/L dNTPs, 2.0 mmol/L Mg2＋, 0.3 mmol/L primer each, 0.3 U Taq polymerase and 1 μl of DNA template. [Conclusion] This system can amplify clear and repeatable DNA profiles, which can be applied for further study about P. hirsutissimum.展开更多
基金Supported by Scientific Research Fund for the Introduced Talents in Hechi University(2008QB-N001)~~
文摘[Objective] This study aimed optimize sequence-related amplified polymor- phism （SRAP）-PCR system for Paphiopedilum hirsutissimum. [Method] Using P. hir- sutissimum leaf as the material, a single-factor test was adopted to optimize the fac- tors in SRAP-PCR system, including the concentrations of dNTPs, Mg2＋, Taq poly- merase, DNA template and primers. [Result] The optimized SRAP-PCR system con- tained 2.5 μl of 10xPCR buffer, 0.15 mmol/L dNTPs, 2.0 mmol/L Mg2＋, 0.3 mmol/L primer each, 0.3 U Taq polymerase and 1 μl of DNA template. [Conclusion] This system can amplify clear and repeatable DNA profiles, which can be applied for further study about P. hirsutissimum.
