Heart failure （HF） is the end stage of various kinds of cardiovascular diseases and leads to a high mortality worldwide. Numerous studies have demonstrated that frequencies of CD4＋CD25＋Foxp3＋ regulatory T cells ...Heart failure （HF） is the end stage of various kinds of cardiovascular diseases and leads to a high mortality worldwide. Numerous studies have demonstrated that frequencies of CD4＋CD25＋Foxp3＋ regulatory T cells （Tregs） are reduced in HF patients and properly expanding Tregs attenuates HF progression. Histone deacetylase （HDAC） 9 has been revealed to contribute to several cardiovascular and cerebrovascular diseases. Plenty of studies showed that HDAC9 negatively regulated the number and function of Tregs. Thus, we aim to investigate the expression of HDAC 9 in patients with chronic heart failure （CHF） and the relationship among HDAC9, Tregs and CHF. Our research showed a reduced number of Tregs and an increased expression of HDAC9 mRNA in CHF patients. Patients with CHF were divided into two groups by heart function grade of New York Heart Association （NYHA）, we found that the HDAC9 mRNA expression level in NYHA grade Ⅱ -Ⅲ group were lower than that in NYHA grade IV group. More importantly, the correlation study suggested that the expression of HDAC9 mRNA was negatively correlated to Tregs frequency and left ventricular ejection fraction （LVEF）, whereas positively correlated to larger left ventricular end-diastolic dimension （LVEDD） and B-type natriuretic peptide （BNP） in patients with CHF. The correlation studies also showed a positive correlation between HDAC9 and the severity of CHF. Our research suggests that HDAC9 may be a new indicator for assessing CHF and it may offer a new direction for research of CHF.展开更多
目的 探究组蛋白去乙酰化酶9（histone deacetylase 9，HDAC9）基因单核苷酸多态性（single nucleotide polymorphism，SNP）rs2107595与冠状动脉粥样硬化严重程度的相关性。 方法 收集1 172例接受冠状动脉造影的患者静脉外周血标本...目的 探究组蛋白去乙酰化酶9（histone deacetylase 9，HDAC9）基因单核苷酸多态性（single nucleotide polymorphism，SNP）rs2107595与冠状动脉粥样硬化严重程度的相关性。 方法 收集1 172例接受冠状动脉造影的患者静脉外周血标本，采用高分辨率溶解曲线方法（HRM）测定rs2107595基因型，并用Gensini评分判定冠状动脉粥样硬化严重程度。 结果 Gensini得分高低在SNP rs2107595 不同基因型组间存在显著差异（GG vs AG vs AA：29（19.5～63） vs 36（19.6～73.1） vs 35（21～69）, P =0.012）。单因素Logistic回归分析显示AA基因型和AG基因型均与Gensini得分风险增高相关（AA vs GG：OR=1.63,95% CI =1.09～ 2.43 ， P =0.018；AG vs GG：OR=1.57,95% CI =1.23～2.00， P =0.001）。多因素Logistic回归分析显示SNP rs2107595基因型、体重、糖尿病和高脂血症是Gensini得分风险增高的独立风险因素。SNP rs2107595与冠心病发病风险的相关性在超重亚组、糖尿病亚组、高脂血症亚组中显著增高。 结论 rs2107595多态性可能是冠状动脉粥样硬化发生、发展的一个危险因素。展开更多
文摘Heart failure （HF） is the end stage of various kinds of cardiovascular diseases and leads to a high mortality worldwide. Numerous studies have demonstrated that frequencies of CD4＋CD25＋Foxp3＋ regulatory T cells （Tregs） are reduced in HF patients and properly expanding Tregs attenuates HF progression. Histone deacetylase （HDAC） 9 has been revealed to contribute to several cardiovascular and cerebrovascular diseases. Plenty of studies showed that HDAC9 negatively regulated the number and function of Tregs. Thus, we aim to investigate the expression of HDAC 9 in patients with chronic heart failure （CHF） and the relationship among HDAC9, Tregs and CHF. Our research showed a reduced number of Tregs and an increased expression of HDAC9 mRNA in CHF patients. Patients with CHF were divided into two groups by heart function grade of New York Heart Association （NYHA）, we found that the HDAC9 mRNA expression level in NYHA grade Ⅱ -Ⅲ group were lower than that in NYHA grade IV group. More importantly, the correlation study suggested that the expression of HDAC9 mRNA was negatively correlated to Tregs frequency and left ventricular ejection fraction （LVEF）, whereas positively correlated to larger left ventricular end-diastolic dimension （LVEDD） and B-type natriuretic peptide （BNP） in patients with CHF. The correlation studies also showed a positive correlation between HDAC9 and the severity of CHF. Our research suggests that HDAC9 may be a new indicator for assessing CHF and it may offer a new direction for research of CHF.
文摘目的 探究组蛋白去乙酰化酶9（histone deacetylase 9，HDAC9）基因单核苷酸多态性（single nucleotide polymorphism，SNP）rs2107595与冠状动脉粥样硬化严重程度的相关性。 方法 收集1 172例接受冠状动脉造影的患者静脉外周血标本，采用高分辨率溶解曲线方法（HRM）测定rs2107595基因型，并用Gensini评分判定冠状动脉粥样硬化严重程度。 结果 Gensini得分高低在SNP rs2107595 不同基因型组间存在显著差异（GG vs AG vs AA：29（19.5～63） vs 36（19.6～73.1） vs 35（21～69）, P =0.012）。单因素Logistic回归分析显示AA基因型和AG基因型均与Gensini得分风险增高相关（AA vs GG：OR=1.63,95% CI =1.09～ 2.43 ， P =0.018；AG vs GG：OR=1.57,95% CI =1.23～2.00， P =0.001）。多因素Logistic回归分析显示SNP rs2107595基因型、体重、糖尿病和高脂血症是Gensini得分风险增高的独立风险因素。SNP rs2107595与冠心病发病风险的相关性在超重亚组、糖尿病亚组、高脂血症亚组中显著增高。 结论 rs2107595多态性可能是冠状动脉粥样硬化发生、发展的一个危险因素。
