AIM: To evaluate the expression pattern of two novel oncofetal antigens, the HoxD9 and Pbx1 homeoproteins in esophageal squamous cell carcinomas (ESCCs) to determine what role they would play in the carcinogenesis of ...AIM: To evaluate the expression pattern of two novel oncofetal antigens, the HoxD9 and Pbx1 homeoproteins in esophageal squamous cell carcinomas (ESCCs) to determine what role they would play in the carcinogenesis of ESCC.METHODS: We obtained tissue samples of ESCC from 56 patients who underwent esophagectomy but not preoperative chemotherapy or radiotherapy. The diagnosis of ESCC was established and confirmed by staff pathologists. We used a highly sensitive, indirect,immunocytochemical method to detect HoxD9 and PbX1 proteins. We qualitatively and quantitively evaluated cells that exhibited and staining using a light microscope.RESULTS: In all observed carcinoma tissue samples, more than 60% of neoplastic cells stained lightly or strongly for HoxD9, and more than 50% of neoplastic cells stained lightly or strongly for Pbx1.CONCLUSION: Our data suggest that HoxD9 and Pbx1 are inappropriately expressed in most human esophageal squamous cell carcinoma. Understanding the role of Hox genes in esophageal epithelial cell carcinogenesis may not only augment early detection but also offer new avenues for treatment of this disease.展开更多
Homeotic genes share a characteristic DNA segment, the homeobox, which encodes a defined domain of the homeotic protein homeodomain which seems to mediate the binding to specific DNA sequences, whereby the homeotic p...Homeotic genes share a characteristic DNA segment, the homeobox, which encodes a defined domain of the homeotic protein homeodomain which seems to mediate the binding to specific DNA sequences, whereby the homeotic protein exerts a gene regulatory function. In this study, the homeodomain encoded by the OSIH 1 (designated from Oryza sativa Indica homeobox 1) homeobox of rice was overproduced in a expression vector in E.coli , as a form of inclusion body and analyzed by Western blotting and crossed immunoreaction. Crossed immunoreaction studies among OSIH 1, Kn 1 and Quox 1 indicate all three are homologous at the protein level. The OSIH 1 homeodomain is then to identify the homeotic target genes.展开更多
基金Supported by Beijing New Star of Science and Technology Plan from Beijing Municipal Science and Technology Commission, No.954813600by Institutional Research Fund of Peking University School of Oncology, Beijing Cancer Hospital and partially supported by Research Fund of Beijing Municipal Science and Technology Commission, No. H020920030390
文摘AIM: To evaluate the expression pattern of two novel oncofetal antigens, the HoxD9 and Pbx1 homeoproteins in esophageal squamous cell carcinomas (ESCCs) to determine what role they would play in the carcinogenesis of ESCC.METHODS: We obtained tissue samples of ESCC from 56 patients who underwent esophagectomy but not preoperative chemotherapy or radiotherapy. The diagnosis of ESCC was established and confirmed by staff pathologists. We used a highly sensitive, indirect,immunocytochemical method to detect HoxD9 and PbX1 proteins. We qualitatively and quantitively evaluated cells that exhibited and staining using a light microscope.RESULTS: In all observed carcinoma tissue samples, more than 60% of neoplastic cells stained lightly or strongly for HoxD9, and more than 50% of neoplastic cells stained lightly or strongly for Pbx1.CONCLUSION: Our data suggest that HoxD9 and Pbx1 are inappropriately expressed in most human esophageal squamous cell carcinoma. Understanding the role of Hox genes in esophageal epithelial cell carcinogenesis may not only augment early detection but also offer new avenues for treatment of this disease.
文摘Homeotic genes share a characteristic DNA segment, the homeobox, which encodes a defined domain of the homeotic protein homeodomain which seems to mediate the binding to specific DNA sequences, whereby the homeotic protein exerts a gene regulatory function. In this study, the homeodomain encoded by the OSIH 1 (designated from Oryza sativa Indica homeobox 1) homeobox of rice was overproduced in a expression vector in E.coli , as a form of inclusion body and analyzed by Western blotting and crossed immunoreaction. Crossed immunoreaction studies among OSIH 1, Kn 1 and Quox 1 indicate all three are homologous at the protein level. The OSIH 1 homeodomain is then to identify the homeotic target genes.
