The energy homeostasis-associated（Enho） gene encodes a secreted protein, adropin, which regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor γ, a major r...The energy homeostasis-associated（Enho） gene encodes a secreted protein, adropin, which regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor γ, a major regulator of lipogenesis. In the present study, the porcine（Sus scrofa） homologue of the Enho gene, which was named p Enho, was amplified by reverse transcriptase polymerase chain reaction（RT-PCR） using oligonucleotide primers derived from in silico sequences. The gene sequence was submitted into the Gen Bank of NCBI, and the access number is GQ414763. The p Enho encodes a protein of 76 amino acids which shows 75% similarity to Homo sapiens adropin. The expression profile of p Enho in tissues（liver, muscle, anterior jejunum, posterior jejunum, and ileum） was determined by quantitative real-time RT-PCR. p Enho was localized on porcine chromosome 10 and no introns were found. In conclusion, p Enho was cloned and analysed with the aim of increasing knowledge about glucose and lipid metabolism in piglets and helping to promote the health and growth of piglets through adropin regulation.展开更多
基金Project supported by the National Natural Science Foundation of China(No.31302003)the Key Program of Hunan Province Scientific and Technology Planning Project(No.2100CK2006)+1 种基金the Special Fund for Innovative and Entrepreneurship Talents of Changsha City(No.K1307006-21)the Key Program of Changsha Scientific and Technology Planning Project(No.K1201108-21),China
文摘The energy homeostasis-associated（Enho） gene encodes a secreted protein, adropin, which regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor γ, a major regulator of lipogenesis. In the present study, the porcine（Sus scrofa） homologue of the Enho gene, which was named p Enho, was amplified by reverse transcriptase polymerase chain reaction（RT-PCR） using oligonucleotide primers derived from in silico sequences. The gene sequence was submitted into the Gen Bank of NCBI, and the access number is GQ414763. The p Enho encodes a protein of 76 amino acids which shows 75% similarity to Homo sapiens adropin. The expression profile of p Enho in tissues（liver, muscle, anterior jejunum, posterior jejunum, and ileum） was determined by quantitative real-time RT-PCR. p Enho was localized on porcine chromosome 10 and no introns were found. In conclusion, p Enho was cloned and analysed with the aim of increasing knowledge about glucose and lipid metabolism in piglets and helping to promote the health and growth of piglets through adropin regulation.
