人鼻咽癌细胞系HONE1的蛋白质双向凝胶电泳分析

目的 :研究人鼻咽癌细胞系HONE1的蛋白质表达特征。方法 :抽提鼻咽癌细胞系HONE1总蛋白 ,双向凝胶电泳 (2 DE)分离、银染显色 ,用凝胶成像仪和PDQUEST软件获取HONE1总蛋白的 2 DE图像 ;根据获得的 2 DE凝胶图像 ,从胶上切取分辨良好、染色较浓的蛋白质点 ,用胰蛋白酶原位水解 ,所获酶解肽段经基质辅助激光解吸飞行时间质谱 (MALDI TOF MS)测定Mr;以测得的肽段Mr为肽质量指纹 (PMF) ,通过PepIdent软件搜索SWISS PROT和TrEMBL数据库而识别该点对应的蛋白质。结果 :建立了双向凝胶电泳分离总蛋白、质谱鉴定银染蛋白质点的方法 ,并鉴定出人鼻咽癌细胞系HONE1中表达较强的 10个点相应的蛋白质。结论 :本研究为建立人鼻咽癌细胞HONE1的 2 DE参考图和蛋白质数据库提供了有用的基础性研究资料 ,为进一步识别鼻咽癌特异性的蛋白质奠定了实验基础。

IMPROVING MATERIAL REMOVAL RATE OF BRITTLE CERAMICS THROUGH HONING INCIDENTAL TENSILE STRESSES

The stress intensity factors and stress conditions of machining cracks are analyzed by fracture mechanics on the basis of honing characteristics and of brittle ceramic mechanical behavior.Because the honing incidental tensile stresses effectively decrease the critical grinding stresses and increase the stress intensity factors of machining cracks,the honing process can be carried out easily.The results show that honing can be an efficient machining method for brittle materials.

一类具有Holling I型反应函数半比例依赖的捕食-食饵离散系统的全局稳定性

主要研究了一类具有Holling I型功能函数半比例依赖的捕食-食饵离散系统的全局稳定性,同时给出了该系统存在一个全局稳定的正周期解的充分条件。

具有HollingⅢ类功能性反应的基于比率的离散Leslie捕食者—食饵模型的持久性

利用差分方程比较原理探讨了周期情形下一类具有HollingⅢ类功能性反应的基于比率的离散Leslie捕食者—食饵模型的持久性,得到了这种模型持久性的充分条件。

具有Holling III类功能反应的三维食物链差分系统周期解的存在性

利用重合度理论研究了一类具有Holling III类功能反应及时滞的三维食物链差分系统全局周期解的存在性,得到了该系统存在严格正周期解的充分条件.

捕食者与被捕食者问题的定性分析

对于一类具有简化HollingIV类功能性反应的扑食与食饵模型做了较为详细的定性分析,得到了系统轨线的全局稳定性,极限环的存在性及系统无环的一些充分条件.部分地改进了文献[2]的一些结果.

Effects of honing treatment on AIP-TiN and TiAlN coated end-mill for high speed machining

The objective of this work is to compare the tool performance of TiN and TiA1N coated carbides end-mills deposited by an arc ion plating （ALP） method, using honing treatment to polish the cutting edge surface sleekly. The curve of surface roughness versus honing time showed a rapid improvement initially and thereafter became steady, manifesting a saturation effect. The optimal honing time related to surface roughness was determined to be approximately 20 s. As the surface roughness increased, the critical loads reduced. At an average surface roughness （Ra） of 0.028 p.m, the highest critical loads of TiN and TiAlN coating layers were 98 and 114 N, respectively. Tool performances of uncoated and coated tools were conducted under high speed machining （HSM） of AISI D2 cold-worked die steel （62 HRC）. Consequently, the TiAlN coated end-mill using honing treatment showed excellent tool life under HSM conditions.

INFLUENCE OF WHEEL STRUCTURAL PARAMETERS ON MACHINING ACCURACY OF ULTRA-PRECISION PLANE HONING

A new idea for designing wheel patterns is presented so as to solve theproblems about machining accuracy of workpiece and wear of honing wheel in ultra-precision planehoning. The influence factors on motion principle and pattern structures are analyzed andoptimization machining parameters are obtained. By calculating effective cutting length on thesurface of workpiece cut by wheel's abrasive and the orbit of one point on the surface of workpiececontacting with wheel, the wear coefficient of different kinds of wheels and accuracy coefficient ofworkpiece machined by corresponding wheels are obtained. Furthermore, the simulation results showthat the optimal pattern structure of wheel turns out to have lower wheel wear and higher machiningaccuracy.

The Experimental and Theoretical Research of "Local Resonance" in Ultrasonic Honing System

In 1982, Professor Fang Guoliang found the "Non full resonance" phenomenon in a tool system while he use the thin-long tool ultrasonically machining deep-small hole. He called it as "local resonance". Also this "Non full resonance" phenomenon was discovered in the ultrasonic drilling and the ultrasonic honing system later. To its mechanism, professor Fang thought that the coupling of long-thin tool bar and driving system is weak, so the tool bar can vibrate independently, but the quantitative relation between the coupling factor and diameter ratio is not made certain. Then several theories come forth to interpret it but still haven’t a common conclusion. Through the systematic experimental and theoretical research, this paper reveals that the "local resonance" phenomenon of ultrasonic honing system has the same essence with the "local resonance" phenomenon in deep hole machining system: when the section area ratio of tool bar and driving system is small enough, some resonance frequencies of combined system are close to the resonance frequencies of "fixed-free" state tool bar, the combined system is still resonant. According to the given depth of hole and structure size, we can use the transfer matrix deduced in this paper to design flexible bar and oilstone seat not only satisfying mechanical structure size but also achieving enough magnitude. It greatly simplified the design. This new method can be named as "local resonance" design method for ultrasonic honing system. The experiment, deduction and design method have a certain common meaning to the study and design of other ultrasonic system.

miR-516b-5p靶向ATM调控鼻咽癌细胞HONE1 DNA修复的机制研究

目的探讨鼻咽癌细胞HONE1中抑制DNA修复的潜在机制。方法使用ETP处理鼻咽癌细胞HONE1造成DNA损伤后,通过高通量测序检测鼻咽癌细胞HONE1中miRNA的表达谱。过表达差异miRNA后,通过基于I-PpoI的可诱导DSB系统检测DNA双链断裂处(DNA double-strand breaks,DSB)的DNA-PKcs含量。通过miRDB在线分析miRNA的潜在靶标。通过荧光素酶报告系统检测miRNA靶向mRNA。结果ETP处理后hsa-miR-9983-3p、hsa-miR-4731-5p、hsa-miR-3675-5p、hsa-miR-516b-5p、hsa-miR-4437、hsa-miR-7114-5p、hsa-miR-637、hsa-miR-5008-5p的表达水平上升至少8倍。miR-516b-5p mimics导致DSB的DNA-PKcs含量减少(P<0.05),而miR-516b-5p inhibitor导致DSB的DNA-PKcs含量增加(P<0.05)。在鼻咽癌细胞HONE1中过表达miR-516b-5p后,ATM mRNA的表达水平和蛋白表达水平下降,DNA-PKcs的磷酸化水平下降;而抑制miR-516b-5p表达后,ATM mRNA的表达水平和蛋白表达水平上升,DNA-PKcs的磷酸化水平上升。同时,miR-516b-5p靶向ATM的3端非编码区。过表达ATM后,DSB的DNA-PKcs含量上升(P<0.05),而敲低ATM后,DSB的DNA-PKcs含量下降(P<0.05)。结论miR-516b-5p通过靶向ATM mRNA的3端非编码区,降解了ATM mRNA,抑制了ATM的翻译,随后DNA-PKcs的磷酸化水平下降,DNA-PKcs与DSB的结合减少,最终抑制了鼻咽癌细胞HONE1的DNA修复。

Optimization of Honing Wheel Structure Parameters in Ultra-precision Plane Honing

Free abrasive particle machining in simple machine such as: honing, polishing can get higher surface finish mirror, but surface error, and working procedure is hard to control. Therefore, the vertical disposed ultra-precision plane honing method by ultra-particle diamond honing wheel is put forward to. The results of experiments indicate: plane-honing wheel has higher machining accuracy and machining efficiency. But at the same time the structure parameters of honing wheel effects on machining accuracy. By analyzing the relation of honing wheel structure parameters and workpiece machining accuracy, the relation of honing wheel and wear coefficient, then this paper gets honing wheel structure parameters in the condition of best accuracy coefficient and wear coefficient, and resolve the problem of choosing honing wheel structure parameters in ultra-precision plane honing at last. This paper analyses the relation of honing wheel structure parameters and workpiece machining accuracy coefficient and wear coefficient, by building relative movement math model of honing wheel and workpiece in plane honing. Through theory calculating, the result indicate: about honing machine tools for large volume manufacture, honing wheel wear is main effect factor, so honing wheel should adopt obverse triangle radial structure. About honing machining for high accuracy and low-batch quantities, machining accuracy coefficient is main factors; so honing wheel should adopt reverse triangle radial structure. Neglected the manufacturing factors of honing wheel, then we can design honing wheel with high power curve structure to meet the need of machining accuracy coefficient and honing wheel wear coefficient in higher accuracy honing.

Analysis of Material Removal in Alumina Ceramic Honing

The removal mechanism is of importance to the grinding of hard and brittle ceramic materials. It is more suitable to analyze the material removal during ceramics honing processes by means of indention fracture approach. There are two honing characteristics different from grinding, the honing incidental tensile stresses and the crosshatch pattern. The stresses may influence material removal of brittle ceramics with lower tensile strength. In addition, the criss-cross cutting pattern on a bore known as crosshatch may also have its effect on the material removal of ceramics. The material removal of alumina is analyzed on the basis of honing characteristics, honing incidental tensile stresses and crosshatch pattern. By means of indentation fracture mechanics of brittle solids, the theoretical analysis of stress intensity factor and the crack response prove that honing incidental tensile stresses can increase the stress intensity factor of honing cracks and decrease the grinding stresses. So, the fracture criterion for crack propagation can be met easily. Therfore, it is possible to machine ceramic materials with small grinding forces in honing processes. The alumina honing experiments show that material at crosshatch intersecting point is removed by way of chipping which is similar to the edge-crumbled of ceramics. For brittle ceramics with lower tensile strength, such as Al 2O 3, SiO 2, the influences of the honing incidental tensile stresses and the crosshatch pattern on material removal are bigger than that of ceramics with relative higher toughness, such as ZrO 2 and Si 3N 4. Hence, the honing of Al 2O 3, SiO 2, is superior in cutting ability to the grinding. The large stock removal of brittle ceramic materials can be obtained through higher honing pressures. The increase in honing pressures can increase intensity factor of honing cracks, decrease the grinding stresses, and remarkably improve material removal rate. The researches show that honing is an efficient bore machining operation for brittle ceramics.

Research on the New Technology and System of Ultrasonic Honing

In recent years, the new technology of ultrasonic vibration cutting has been drawing general attention. And great advancement in theory and applications in production of the technology have been achieved. Ultrasonic vibration honing system developed in North China Institute of Technology and Nanjing University of Aeronautics and Astronautics of cast iron cylinder (47×97) and thin cylinder of 20# (86×95) has been successfully applied to productive experiment and the technological effect obtained is ideal. In comparison with ordinary honing, ultrasonic vibration honing possesses apparent characteristics: Firstly, it has high processing precision (precision in size ranks IT6, roughness Ra0.05, circularty and cylindericity in size ranks IT6); Secondly, it has small cutting force and low temperature so that no deformation occurs; Thirdly, it has high efficiency. In this paper, the application of a new technique to honing is introduced, and the ultrasonic honing systems of thin cylinder and cast cylinder which have been successfully manufactured and their applying effect are illustrated. This paper analyzes the cutting principle of ultrasonic honing in terms of cutting motion, grain performance and cutting quantity. The study of this technique starts a new approach to which is efficient higher quality and lower consumption to hole’s precise and superprecise machining. At last,the paper introduce the conclution: Ultrasonic honing sharpens grain, which is favorable for raising cutting efficiency; Ultrasonic vibration honing may increase honing depth and raise cutting efficiency. It may also decrease honing power and system deformation; Undergoing practice we can find that the ultrasonic honing system as above discribed can be extended and applied to production. The system not only can be applied to conventional honing machine, its technology effect as good as high precision machine tool, but also can be applied to develop and study ultrasonic vibrating numbrical controlling honing machine tool which will provide a new type, efficient, high quality low consumption, equipment for working precision hole; Appling the same parament as W40 oilstone bar, under the same condition, is very valuable for product and application. Rough machining and finish machining once time finished, which not only improve location accuracy, but also have a high effiency.The honing system not only can be suitable for honing cast cylinder, but also can be applied for honing engineer ceramics as well as hard, crisp material; The dimension and technology paraments of 47 mm honing system as above discribed, can be suitable for honing the cast cylinder of motorcycle or minicar. And the cylinder diameter can be randomly selected from 45 to 65. The dimension and technology paramagnets of 86 honing system as above discribed,can be applied for honing all kinds of cylinder of light or heavy passenger-cargo vehicle, and the cylinder diameter can be randomly selected from 65 to 100; It is easy to achieve new technology of flat roof honing by applying ultrasonic honing.After the flat roof honing, fuel consumption of engine is 1g/Hp, while conventional engine is 3g/Hp.Life expectancy of engine is prolonged greatly after the cylinder by flat roof honing.

Examination and Research of the Surface Topography of Ultrasonic Vibration Honing Nd-Fe-B

The mechanism of ultrasonic vibration honing Nd-Fe-B has been briefly elaborated after the introduction of the strategic significance of processing Nd-Fe-B. Based on the formation principle of Scanning Electrtmic Microscope （SEM）, and at the examination with the aid of SEM to the ultrasonic vibration honing Nd-Fe-B material＇s superficial microscopic topography, the paper discusses the new processing nechanism according to the SEM examination picture. The research indicates that as a result of supersonic high frequency vibration, the path of the abrasion extends at the same time, and the supersonic cavitation effect forms the intense shock-wave, knpacting Nd-Fe-B material＇s intemal surface, providing the supersonic energy for the superticial abrasive dust＇s dimination, which directly explain that the honing processing efficiency is enhanced, and the processing surface roughness is high.

Brutal Honesty

In reality,it is easier and better to be honest.One of my favourite,and least favourite characteristics of my Chinese friends is that they are extremely honest.In the West,we spend a lot of time being extremely care-ful what we say to each other,and we also like to make sure that we are always

Comparison of Different Approaches to Force Controlled Precision Honing of Bores

Honing is a machining process which can economically produce exact bores regarding form, geometry and surface quality. During the honing process, the tool, equipped with one or several abrasive honing stones, combines three movement components： rotation, oscillation in axial direction and radial feed movement of the honing stone. The feed movement is a decisive factor for the results of the honing process and can be controlled either by a gradual lining in fixed time intervals or by the regulation of the occurring forces. The presented studies show different approaches for the regulation of force controlled honing and their effects on the measured forces, the torque and the quality parameters of the process.

Honing Farming Skills

ANIMAL husbandry technician Vasco Silva always found it difficult to master the technology applied to artificial insemination （AI） of cattle. However, in December last year. a training course changed the Mozambican＇s original thought of the technology and the way it is used,

具有非线性密度制约的HollingⅡ类功能性反应的食饵与捕食者模型的定性分析

研究一类具有非线性密度制约的HollingⅡ型功能性反应的食饵-捕食者系统:x′=xg(x)-yφ(x),y′=y(-d+kφ(x))在g(x)=a-b x的情况下.分析了该系统的平衡点性态,证明了系统在正平衡点的外围极限环的存在性,得出了在一定的条件下,正平衡点外围至少有2或者3个极限环的结论.

海带多糖和茶多酚对鼻咽癌细胞HONE1和CNE2增殖的影响

目的:观察海带多糖(LJP)和茶多酚(TP)单独及联合应用对鼻咽癌(NPC)细胞HONE1和CNE2增殖的影响,了解LJP对体内移植瘤的抑制作用,探讨其抗癌机制。方法:以NPC细胞HONE1和CNE2为研究对象,绘制两者生长曲线,计算倍增时间确定对数生长期细胞,采用MTT检测LJP和TP单独及联合应用对两组细胞的增殖抑制率,运用Annexin V加PI双染法检测LJP对HONE1细胞凋亡的影响;以人NPC细胞株HONE1建立裸鼠皮下移植瘤模型及行体内抑瘤实验。结果:LJP和TP均对两组细胞有增殖抑制作用,LJP和TP联合应用对两组实验细胞抑制作用均高于两者单独应用的效果,且这种作用呈浓度依赖关系。流式细胞仪检测结果显示,LJP具有诱导HONE1细胞凋亡的作用,凋亡率随着LJP浓度的增加而增高,320mg/L的凋亡率为(49.51±1.89)%(P<0.01)。体内实验中,25.0mg/kg LJP组、50.0mg/kg LJP组的抑瘤率分别为33.7%(P<0.05)和47.0%(P<0.01),具有明显抑制移植瘤的作用,而12.5mg/kg LJP组的抑瘤率仅为16.4%(P>0.05)。结论:LJP和TP对鼻咽癌细胞HONE1和CNE2增殖均有抑制作用,两者联合应用更具有显著效果,LJP作用机制可能是通过诱导癌细胞凋亡而实现的。

冬凌草甲素影响细胞外排功能增加HONE1细胞对米托蒽醌的敏感性

目的观察非毒性剂量冬凌草甲素是否增加鼻咽癌细胞株HONE1对米托蒽醌的敏感性。方法采用MTT法使用不同浓度的冬凌草甲素处理HONE1细胞株,确定冬凌草甲素的非毒剂量区间;然后用非毒性剂量冬凌草处理细胞后用流式细胞术进行侧群表型检测,探索能显著降低侧群表型的冬凌草甲素浓度;参考冬凌草甲素非毒性剂量和能降低侧群表型的剂量得到冬凌草甲素用于联用米托蒽醌的浓度。结果 5μmol/L冬凌草甲素并未对HONE1细胞产生明显毒性,该浓度处理细胞24 h后,细胞侧群比例由3.32%降低至0.55%;米托蒽醌联用5μmol/L冬凌草甲素组HONE1细胞存活率较单用米托蒽醌组低(P<0.05);流式细胞术分析显示膜外排蛋白ABCG2含量降低。结论冬凌草甲素可以通过影响细胞ABCG2外排功能增加鼻咽癌细胞株HONE1对米托蒽醌的敏感性。