Simple sequences repeat (SSR) molecular maker, as a new type of DNA molecular marker, the second generation based on the polymerase chain reaction (PCR), is valuable and of great potential as genetic markers for i...Simple sequences repeat (SSR) molecular maker, as a new type of DNA molecular marker, the second generation based on the polymerase chain reaction (PCR), is valuable and of great potential as genetic markers for its characteristics of abundant quantity, high polymorphic, reproducibility, specific site amplification, high occurring frequency, and co-dominant inheritance etc. This paper outlined its principles and characteristics, and introduced its application to variety identification, phylogenetic relationship analysis, genetic diversity analysis, DNA fingerprinting and linkage map constructing etc. in recent years in Citrus and its close relatives.展开更多
文摘Simple sequences repeat (SSR) molecular maker, as a new type of DNA molecular marker, the second generation based on the polymerase chain reaction (PCR), is valuable and of great potential as genetic markers for its characteristics of abundant quantity, high polymorphic, reproducibility, specific site amplification, high occurring frequency, and co-dominant inheritance etc. This paper outlined its principles and characteristics, and introduced its application to variety identification, phylogenetic relationship analysis, genetic diversity analysis, DNA fingerprinting and linkage map constructing etc. in recent years in Citrus and its close relatives.