Zebrafish(D anio rerio)have attracted much attention over the past decade as a reliable model for gut microbiome research.Owing to their low cost,strong genetic and development coherence,efficient preparation of germ-...Zebrafish(D anio rerio)have attracted much attention over the past decade as a reliable model for gut microbiome research.Owing to their low cost,strong genetic and development coherence,efficient preparation of germ-f ree(GF)larvae,availability in high-t hroughput chemical screening,and fitness for intravital imaging in vivo,zebrafish have been extensively used to investigate microbiome-h ost interactions and evaluate the toxicity of environmental pollutants.In this review,the advantages and disadvantages of zebrafish for studying the role of the gut microbiome compared with warm-b looded animal models are first summarized.Then,the roles of zebrafish gut microbiome on host development,metabolic pathways,gut-b rain axis,and immune disorders and responses are addressed.Furthermore,their applications for the toxicological assessment of aquatic environmental pollutants and exploration of the molecular mechanism of pathogen infections are reviewed.We highlight the great potential of the zebrafish model for developing probiotics for xenobiotic detoxification,resistance against bacterial infection,and disease prevention and cure.Overall,the zebrafish model promises a brighter future for gut microbiome research.展开更多
基金supported by the National Natural Science Foundation of China(31970777)Discipline Construction Project of Guangdong Medical University(4SG21014G,4SG21003G)the Natural Science Foundation of Guangdong Province(2020A151501457).
文摘Zebrafish(D anio rerio)have attracted much attention over the past decade as a reliable model for gut microbiome research.Owing to their low cost,strong genetic and development coherence,efficient preparation of germ-f ree(GF)larvae,availability in high-t hroughput chemical screening,and fitness for intravital imaging in vivo,zebrafish have been extensively used to investigate microbiome-h ost interactions and evaluate the toxicity of environmental pollutants.In this review,the advantages and disadvantages of zebrafish for studying the role of the gut microbiome compared with warm-b looded animal models are first summarized.Then,the roles of zebrafish gut microbiome on host development,metabolic pathways,gut-b rain axis,and immune disorders and responses are addressed.Furthermore,their applications for the toxicological assessment of aquatic environmental pollutants and exploration of the molecular mechanism of pathogen infections are reviewed.We highlight the great potential of the zebrafish model for developing probiotics for xenobiotic detoxification,resistance against bacterial infection,and disease prevention and cure.Overall,the zebrafish model promises a brighter future for gut microbiome research.
