本文深入剖析了“对分课堂”教学模式,并在高等职业教育领域成功实践了“136”教学模式。该模式以学生为中心,倡导主动性、合作性和探究性,通过创新的教学组织形式,有效激发了学生的学习动力和创造潜力。文章细致阐释了“136”模式的框...本文深入剖析了“对分课堂”教学模式,并在高等职业教育领域成功实践了“136”教学模式。该模式以学生为中心,倡导主动性、合作性和探究性,通过创新的教学组织形式,有效激发了学生的学习动力和创造潜力。文章细致阐释了“136”模式的框架结构,包括其核心理念、三个关键教学阶段和六个基本步骤,这些设计旨在促进学生的深度学习和全面发展。研究结果显示,“136”模式显著提升了学生的课堂参与度和学业成绩。通过与传统教学模式的对比分析,新模式下学生的答题正确率和知识接收率均有大幅提升,这一变化客观地反映了教学效果的显著改进。This paper deeply analyzes the teaching mode of “divided classroom” and successfully practices the “136” teaching mode in the field of higher vocational education. This model is student-centered, advocates initiative, cooperation and inquiry, and effectively stimulates students’ learning motivation and creative potential through innovative teaching organization forms. The article carefully explains the framework structure of the “136” mode, including its core ideas, three key teaching stages and six basic steps, which are designed to promote students’ deep learning and all-round development. The results show that the “136” mode significantly improves students’ classroom engagement and academic performance. Through the comparative analysis with the traditional teaching mode, the correct answer rate and knowledge reception rate of students in the new mode have been greatly improved, which objectively reflects the significant improvement of the teaching effect.展开更多
Background:Glioblastoma remains a highly invasive primary brain malignancy with an undesirable prognosis.Growing evidence has shed light on the importance of microRNAs(miRs),as small non-coding RNAs,in tumor developme...Background:Glioblastoma remains a highly invasive primary brain malignancy with an undesirable prognosis.Growing evidence has shed light on the importance of microRNAs(miRs),as small non-coding RNAs,in tumor development and progression.The present study leverages the in-silico and in-vitro techniques to investigate the significance of hsa-miR-181a-5p and the underlying hsa-miR-181a-5p-meidated signaling pathway in glioblastoma development.Methods:Bioinformatic studies were performed on GSE158284,GSE108474(REMBRANDT study),TCGA-GTEx,CCLE,GeneMANIA,Reactome,WikiPathways,KEGG,miRDB,and microT-CDS to identify the significance of hsa-miR-181a-5p and its underlying target.Afterward,the U373 cell line was selected and transfected with hsa-miR-181a-5p mimics,and the cell viability,clonogenicity,migration,mRNA expression,apoptosis,and cell cycle were studied using the MTT assay,colony formation test,migration assay,qRT-PCR,andflow cytometry respectively.Results:hsa-miR-181a-5p expression is decreased in glioblastoma samples.The in-silico results have shown that hsa-miR-181a-5p could regulate the MAPK pathway by targeting AKT3.The experimental assays have shown that hsa-miR-181a-5p decreases the migration of glioblastoma cells,arrests the cell cycle,and increases the apoptosis rate.Besides downregulating MMP9 and upregulating BAX,hsa-miR-181a-5p downregulates MET,MAP2K1,MAPK1,MAPK3,and AKT3 expression in U373 cells.The in-vitro results were consistent with in-silico results regarding the regulatory effect of hsa-miR-181a-5p on the MAPK pathway,leading to tumor suppression in glioblastoma.Conclusions:hsa-miR-181a-5p inhibits glioblastoma development partially by regulating the signaling factors of the MAPK pathway.展开更多
文摘本文深入剖析了“对分课堂”教学模式,并在高等职业教育领域成功实践了“136”教学模式。该模式以学生为中心,倡导主动性、合作性和探究性,通过创新的教学组织形式,有效激发了学生的学习动力和创造潜力。文章细致阐释了“136”模式的框架结构,包括其核心理念、三个关键教学阶段和六个基本步骤,这些设计旨在促进学生的深度学习和全面发展。研究结果显示,“136”模式显著提升了学生的课堂参与度和学业成绩。通过与传统教学模式的对比分析,新模式下学生的答题正确率和知识接收率均有大幅提升,这一变化客观地反映了教学效果的显著改进。This paper deeply analyzes the teaching mode of “divided classroom” and successfully practices the “136” teaching mode in the field of higher vocational education. This model is student-centered, advocates initiative, cooperation and inquiry, and effectively stimulates students’ learning motivation and creative potential through innovative teaching organization forms. The article carefully explains the framework structure of the “136” mode, including its core ideas, three key teaching stages and six basic steps, which are designed to promote students’ deep learning and all-round development. The results show that the “136” mode significantly improves students’ classroom engagement and academic performance. Through the comparative analysis with the traditional teaching mode, the correct answer rate and knowledge reception rate of students in the new mode have been greatly improved, which objectively reflects the significant improvement of the teaching effect.
文摘Background:Glioblastoma remains a highly invasive primary brain malignancy with an undesirable prognosis.Growing evidence has shed light on the importance of microRNAs(miRs),as small non-coding RNAs,in tumor development and progression.The present study leverages the in-silico and in-vitro techniques to investigate the significance of hsa-miR-181a-5p and the underlying hsa-miR-181a-5p-meidated signaling pathway in glioblastoma development.Methods:Bioinformatic studies were performed on GSE158284,GSE108474(REMBRANDT study),TCGA-GTEx,CCLE,GeneMANIA,Reactome,WikiPathways,KEGG,miRDB,and microT-CDS to identify the significance of hsa-miR-181a-5p and its underlying target.Afterward,the U373 cell line was selected and transfected with hsa-miR-181a-5p mimics,and the cell viability,clonogenicity,migration,mRNA expression,apoptosis,and cell cycle were studied using the MTT assay,colony formation test,migration assay,qRT-PCR,andflow cytometry respectively.Results:hsa-miR-181a-5p expression is decreased in glioblastoma samples.The in-silico results have shown that hsa-miR-181a-5p could regulate the MAPK pathway by targeting AKT3.The experimental assays have shown that hsa-miR-181a-5p decreases the migration of glioblastoma cells,arrests the cell cycle,and increases the apoptosis rate.Besides downregulating MMP9 and upregulating BAX,hsa-miR-181a-5p downregulates MET,MAP2K1,MAPK1,MAPK3,and AKT3 expression in U373 cells.The in-vitro results were consistent with in-silico results regarding the regulatory effect of hsa-miR-181a-5p on the MAPK pathway,leading to tumor suppression in glioblastoma.Conclusions:hsa-miR-181a-5p inhibits glioblastoma development partially by regulating the signaling factors of the MAPK pathway.
