目的:评估急诊冠状动脉介入治疗术(PCI)后循环microRNA-26b-5p水平和血糖水平变化的趋势及相关性。方法:根据入选标准和排除标准,共入选接受急诊PCI术的急性前壁心肌梗死患者38例。详细记录临床相关资料,分别于术前、术后24h、术后72h...目的:评估急诊冠状动脉介入治疗术(PCI)后循环microRNA-26b-5p水平和血糖水平变化的趋势及相关性。方法:根据入选标准和排除标准,共入选接受急诊PCI术的急性前壁心肌梗死患者38例。详细记录临床相关资料,分别于术前、术后24h、术后72h行循环血microRNA-26b-5p和血糖水平检测。Real Time PCR法检测血液样本中microRNA-26b-5p表达量的变化。结果:对于成功行急诊PCI的急性心肌梗死患者,术后microRNA-26b-5p表达水平较术前明显升高(P<0.01),术后血糖水平逐渐下降(P<0.01),两者之间存在负相关(r=-0.332,P<0.01)。结论:急性心肌梗死患者往往存在应激性高血糖,microRNA-26b-5p可能参与血糖水平的相关。展开更多
目的检测子宫肌瘤患者血清和组织中hsa-miR-15b-5p和hsa-miR-21-5p水平,并分析其与组织中雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)蛋白之间的相关性,进而探讨子宫肌瘤发生发展的作用机制。方法选取2019...目的检测子宫肌瘤患者血清和组织中hsa-miR-15b-5p和hsa-miR-21-5p水平,并分析其与组织中雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)蛋白之间的相关性,进而探讨子宫肌瘤发生发展的作用机制。方法选取2019年8月~2020年12月于陆军军医大学士官学校附属医院妇科就诊的60例子宫肌瘤患者作为研究对象,另取同期因子宫脱垂行切除术的60例患者作为对照,通过免疫组化SP法和Westerblot法检测所有患者肌瘤组织和正常肌层组织中ER和PR蛋白的相对表达;术前采所有患者的空腹外周静脉血,通过qRT-PCR法分别检测患者血清和组织中hsa-miR-15b-5p和hsa-miR-21-5p mRNA的相对表达水平,并分析组织中hsa-miR-15b-5p和hsa-miR-21-5p水平与ER,PR蛋白表达的相关性。结果免疫组化结果显示,ER蛋白在子宫肌瘤组和正常子宫肌层组的阳性表达率分别为73.3%(44/60)和38.3%(23/60)(χ^(2)=14.903,P=0.002),PR蛋白在子宫肌瘤组和正常子宫肌层组阳性表达率分别为65.0%(39/60)和31.7%(19/60)(χ^(2)=13.348,P=0.005),差异均有统计学意义。Westernblot结果显示,子宫肌瘤组ER水平(0.98±0.21)较正常子宫肌层组(0.62±0.15)显著升高(t=22.147,P<0.001),子宫肌瘤组PR水平(0.80±0.14)较正常子宫肌层组(0.38±0.08)亦显著升高(t=38.265,P<0.001),差异均有统计学意义。qRT-PCR结果表明,与正常子宫肌层组相比,hsa-miR-15b-5p(1.98±0.12 vs 1.02±0.11)和hsa-miR-21-5p(2.21±0.15 vs 0.93±0.18)mRNA在子宫肌瘤患者血清中表达水平均显著增加(t=46.080,63.944,均P<0.001),在肌瘤组织中两者表达水平较正常肌层组织亦显著升高(t=39.852,52.046,均P<0.001),差异均有统计学意义。Pearson法分析发现,组织中hsa-miR-15b-5p和hsa-miR-21-5p表达水平与ER和PR蛋白表达水平呈明显正相关(r=0.744~0.902,均P<0.001)。结论hsa-miR-15b-5p和hsa-miR-21-5p在子宫肌瘤患者血清和组织中表达水平均显著升高,子宫肌瘤组织中ER和PR蛋白亦呈高表达,并且hsa-miR-15b-5p和hsa-miR-21-5p水平与ER和PR表达水平呈明显正相关,可能协同诱导子宫肌瘤发生发展。展开更多
目的:筛选胰腺癌(PC)侵袭相关微小RNA(miRNA,miR),验证其生物学功能和调控的信号通路,为胰腺癌的诊治提供新的分子标志物和靶点。方法:通过基因表达综合数据库(GEO)的miRNA表达谱数据集GSE71533和GSE85589,分析miR-26b-5p的差异表达;利...目的:筛选胰腺癌(PC)侵袭相关微小RNA(miRNA,miR),验证其生物学功能和调控的信号通路,为胰腺癌的诊治提供新的分子标志物和靶点。方法:通过基因表达综合数据库(GEO)的miRNA表达谱数据集GSE71533和GSE85589,分析miR-26b-5p的差异表达;利用癌症基因组图谱数据库(TCGA),分析miR-26b-5p对PC患者总生存率的影响。采用TargetScan数据库识别miR-26b-5p的靶基因集。通过细胞功能实验,验证miR-26b-5p对PANC-1细胞增殖、迁移和侵袭的影响。利用基因本体(GO)和KEGG通路富集分析,揭示miR-26b-5p靶基因的功能,并通过蛋白印迹(Western blotting)实验验证miR-26b-5p靶向的信号通路。结果:在GSE71533和GSE85589数据集,相对于正常组织和对照组血浆,miR-26b-5p在PC组织和PC患者血浆明显低表达(9.65±0.10 vs 10.18±0.07,P<0.001;0.67±0.18 vs 0.79±0.24,P=0.017)。TCGA-胰腺癌生存分析结果显示,miR-26b-5p高表达组的总体生存率明显优于低表达组(P=0.023)。KEGG分析显示,预测的251个miR-26b-5p的靶基因与MAPK信号通路有关。CCK-8实验、Transwell迁移、侵袭实验和划痕实验结果显示:miR-26b-5p抑制PANC-1细胞的增殖、迁移和侵袭。KEGG通路富集分析结果提示miR-26b-5p的靶基因参与丝裂原活化蛋白激酶(MAPK)信号通路。Western blotting实验证实miR-26b-5p下调MKNK2/eIF4E信号通路。结论:miR-26b-5p下调MKNK2/eIF4E信号通路,抑制PANC-1细胞增殖、侵袭,有可能作为胰腺癌诊断和治疗的新靶点。展开更多
Systemic sclerosis (SSc) is a highly heterogeneous autoimmune disease with a high mortality rate.However,the cellular and molecular mechanisms of SSc remain unclear.Here,we identified the key hub genes and microRNAs (...Systemic sclerosis (SSc) is a highly heterogeneous autoimmune disease with a high mortality rate.However,the cellular and molecular mechanisms of SSc remain unclear.Here,we identified the key hub genes and microRNAs (miRNAs) that modulate the occurrence and development of SSc.We downloaded the microarray dataset GSE95065 from the Gene Expression Omnibus (GEO) database and then analyzed the data by using GEO2R.The Database for Annotation,Visualization and Integrated Discovery (DAVID) was used for functional pathway enrichment analyses of differentially expressed genes (DEGs),and Cytoscape software was used to generate the protein-protein interaction (PPI) network.In addition,OmicsNet was used to predict the miRNAs for the hub genes of SSc.As a result,783 DEGs were identified,of which 770 genes (142 up-regulated genes and 628 down-regulated genes) were matched to the genes in SSc skin samples.Gene Ontology (GO) analyses by DAVID indicated that the up-regulated genes were mainly involved in immune response,and the down-regulated genes were greatly enriched in glycinergic synaptic transmission.In the PPI network,22 nodes were selected as key genes,including several members of the chemokine family.Furthermore,after uploading these key genes to the OmicsNet tool,we found that hsa-miR-26b-5p might target CXCL9 and CXCL13.Moreover,we demonstrated that the hsa-miR-26b-5p inhibitor might inhibit fibrosis in TGF-β-activated fibroblasts,which would be a promising target for SSc therapy.展开更多
文摘目的:评估急诊冠状动脉介入治疗术(PCI)后循环microRNA-26b-5p水平和血糖水平变化的趋势及相关性。方法:根据入选标准和排除标准,共入选接受急诊PCI术的急性前壁心肌梗死患者38例。详细记录临床相关资料,分别于术前、术后24h、术后72h行循环血microRNA-26b-5p和血糖水平检测。Real Time PCR法检测血液样本中microRNA-26b-5p表达量的变化。结果:对于成功行急诊PCI的急性心肌梗死患者,术后microRNA-26b-5p表达水平较术前明显升高(P<0.01),术后血糖水平逐渐下降(P<0.01),两者之间存在负相关(r=-0.332,P<0.01)。结论:急性心肌梗死患者往往存在应激性高血糖,microRNA-26b-5p可能参与血糖水平的相关。
文摘目的检测子宫肌瘤患者血清和组织中hsa-miR-15b-5p和hsa-miR-21-5p水平,并分析其与组织中雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)蛋白之间的相关性,进而探讨子宫肌瘤发生发展的作用机制。方法选取2019年8月~2020年12月于陆军军医大学士官学校附属医院妇科就诊的60例子宫肌瘤患者作为研究对象,另取同期因子宫脱垂行切除术的60例患者作为对照,通过免疫组化SP法和Westerblot法检测所有患者肌瘤组织和正常肌层组织中ER和PR蛋白的相对表达;术前采所有患者的空腹外周静脉血,通过qRT-PCR法分别检测患者血清和组织中hsa-miR-15b-5p和hsa-miR-21-5p mRNA的相对表达水平,并分析组织中hsa-miR-15b-5p和hsa-miR-21-5p水平与ER,PR蛋白表达的相关性。结果免疫组化结果显示,ER蛋白在子宫肌瘤组和正常子宫肌层组的阳性表达率分别为73.3%(44/60)和38.3%(23/60)(χ^(2)=14.903,P=0.002),PR蛋白在子宫肌瘤组和正常子宫肌层组阳性表达率分别为65.0%(39/60)和31.7%(19/60)(χ^(2)=13.348,P=0.005),差异均有统计学意义。Westernblot结果显示,子宫肌瘤组ER水平(0.98±0.21)较正常子宫肌层组(0.62±0.15)显著升高(t=22.147,P<0.001),子宫肌瘤组PR水平(0.80±0.14)较正常子宫肌层组(0.38±0.08)亦显著升高(t=38.265,P<0.001),差异均有统计学意义。qRT-PCR结果表明,与正常子宫肌层组相比,hsa-miR-15b-5p(1.98±0.12 vs 1.02±0.11)和hsa-miR-21-5p(2.21±0.15 vs 0.93±0.18)mRNA在子宫肌瘤患者血清中表达水平均显著增加(t=46.080,63.944,均P<0.001),在肌瘤组织中两者表达水平较正常肌层组织亦显著升高(t=39.852,52.046,均P<0.001),差异均有统计学意义。Pearson法分析发现,组织中hsa-miR-15b-5p和hsa-miR-21-5p表达水平与ER和PR蛋白表达水平呈明显正相关(r=0.744~0.902,均P<0.001)。结论hsa-miR-15b-5p和hsa-miR-21-5p在子宫肌瘤患者血清和组织中表达水平均显著升高,子宫肌瘤组织中ER和PR蛋白亦呈高表达,并且hsa-miR-15b-5p和hsa-miR-21-5p水平与ER和PR表达水平呈明显正相关,可能协同诱导子宫肌瘤发生发展。
文摘目的:筛选胰腺癌(PC)侵袭相关微小RNA(miRNA,miR),验证其生物学功能和调控的信号通路,为胰腺癌的诊治提供新的分子标志物和靶点。方法:通过基因表达综合数据库(GEO)的miRNA表达谱数据集GSE71533和GSE85589,分析miR-26b-5p的差异表达;利用癌症基因组图谱数据库(TCGA),分析miR-26b-5p对PC患者总生存率的影响。采用TargetScan数据库识别miR-26b-5p的靶基因集。通过细胞功能实验,验证miR-26b-5p对PANC-1细胞增殖、迁移和侵袭的影响。利用基因本体(GO)和KEGG通路富集分析,揭示miR-26b-5p靶基因的功能,并通过蛋白印迹(Western blotting)实验验证miR-26b-5p靶向的信号通路。结果:在GSE71533和GSE85589数据集,相对于正常组织和对照组血浆,miR-26b-5p在PC组织和PC患者血浆明显低表达(9.65±0.10 vs 10.18±0.07,P<0.001;0.67±0.18 vs 0.79±0.24,P=0.017)。TCGA-胰腺癌生存分析结果显示,miR-26b-5p高表达组的总体生存率明显优于低表达组(P=0.023)。KEGG分析显示,预测的251个miR-26b-5p的靶基因与MAPK信号通路有关。CCK-8实验、Transwell迁移、侵袭实验和划痕实验结果显示:miR-26b-5p抑制PANC-1细胞的增殖、迁移和侵袭。KEGG通路富集分析结果提示miR-26b-5p的靶基因参与丝裂原活化蛋白激酶(MAPK)信号通路。Western blotting实验证实miR-26b-5p下调MKNK2/eIF4E信号通路。结论:miR-26b-5p下调MKNK2/eIF4E信号通路,抑制PANC-1细胞增殖、侵袭,有可能作为胰腺癌诊断和治疗的新靶点。
基金This project was supported by National Natural Science Foundation of China (No.81472886).
文摘Systemic sclerosis (SSc) is a highly heterogeneous autoimmune disease with a high mortality rate.However,the cellular and molecular mechanisms of SSc remain unclear.Here,we identified the key hub genes and microRNAs (miRNAs) that modulate the occurrence and development of SSc.We downloaded the microarray dataset GSE95065 from the Gene Expression Omnibus (GEO) database and then analyzed the data by using GEO2R.The Database for Annotation,Visualization and Integrated Discovery (DAVID) was used for functional pathway enrichment analyses of differentially expressed genes (DEGs),and Cytoscape software was used to generate the protein-protein interaction (PPI) network.In addition,OmicsNet was used to predict the miRNAs for the hub genes of SSc.As a result,783 DEGs were identified,of which 770 genes (142 up-regulated genes and 628 down-regulated genes) were matched to the genes in SSc skin samples.Gene Ontology (GO) analyses by DAVID indicated that the up-regulated genes were mainly involved in immune response,and the down-regulated genes were greatly enriched in glycinergic synaptic transmission.In the PPI network,22 nodes were selected as key genes,including several members of the chemokine family.Furthermore,after uploading these key genes to the OmicsNet tool,we found that hsa-miR-26b-5p might target CXCL9 and CXCL13.Moreover,we demonstrated that the hsa-miR-26b-5p inhibitor might inhibit fibrosis in TGF-β-activated fibroblasts,which would be a promising target for SSc therapy.
