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hsp65基因序列PCR-RFLP法鉴定130株临床分枝杆菌研究 被引量:3
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作者 刘佳文 吴雪琼 杨京灵 《临床肺科杂志》 2006年第4期453-454,共2页
目的探讨hsp65基因序列PCR-RFLP法用于临床分枝杆菌鉴定的诊断价值。方法用hsp65基因序列PCR-RFLP法鉴定经过传统方法鉴定过的130株临床分枝杆菌。结果130株临床分枝杆菌中,鉴定出结核分枝杆菌105株,戈登分枝杆菌4株、瘰痢分枝杆菌3株... 目的探讨hsp65基因序列PCR-RFLP法用于临床分枝杆菌鉴定的诊断价值。方法用hsp65基因序列PCR-RFLP法鉴定经过传统方法鉴定过的130株临床分枝杆菌。结果130株临床分枝杆菌中,鉴定出结核分枝杆菌105株,戈登分枝杆菌4株、瘰痢分枝杆菌3株、耻垢分枝杆菌1株。用PCR-RFLP法,偶然分枝杆菌复合群和鸟胞分枝杆菌复合群进一步鉴定,偶然分枝杆菌2株、脓肿分枝杆菌2株、龟分枝杆菌4株、鸟分枝杆菌5株、胞分枝杆菌4株。结论hsp65基因序列PCR-RFLP法比传统方法有更高的准确性、敏感性、特异性和重复性,具有很好的临床应用价值。 展开更多
关键词 hsp65基因 PCR-RFLP 分枝杆菌
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PCR-RFLP Methodology to Identify <i>Nocardia</i>Isolates in Cuba 被引量:1
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作者 Yaxsier de Armas Carlos Manuel Fernández +3 位作者 Raúl Díaz Nicolas Bioni Stefano Gustavo Wissmann Enrique José Calderón 《Advances in Microbiology》 2015年第10期724-729,共6页
Nocardiosis diagnosis is a major challenge. The clinical features and radiological findings are nonspecific. Traditionally, Nocardia identification is based on colonial and microscopical morphology and biochemical tes... Nocardiosis diagnosis is a major challenge. The clinical features and radiological findings are nonspecific. Traditionally, Nocardia identification is based on colonial and microscopical morphology and biochemical tests. However, molecular biology techniques allow a better characterization of species and biotypes. PCR-RFLP of the 65-kDa heat shock protein (HSP) gene provides a rapid, sensitive, and time and labor-efficient method for this proposal. Using this technique, six of eight isolates tested were identified as Nocardia asteroides type VI. PCR-RFLP of the 65-kDa HSP gene could be very useful for determining the incidence of this pathogen in different population groups and its association with susceptibility/resistance profiles to the drugs of choice for treatment. This work is the first molecular detection of Nocardia species in Cuba. 展开更多
关键词 NOCARDIA PCR-RFLP CUBA Molecular Identification 65-kDa hsp gene
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