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克鲁维酵母(K. hubeiensis)产菊粉酶的发酵参数研究
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作者 周伏忠 陈晓飞 +2 位作者 陈国参 李军旗 宁萌 《河南科学》 2011年第4期416-421,共6页
菊粉酶(inulinase)是一种降解菊糖β-2,1-D-果聚糖果糖苷键生成果糖或低聚果糖的水解酶.本研究在筛选菊粉酶高产菌株基础上,对产酶能力较强的1株湖北克鲁维酵母(Kluyvreromyces hubeiensis)的发酵产酶参数进行了优化;结果表明,该菌株在... 菊粉酶(inulinase)是一种降解菊糖β-2,1-D-果聚糖果糖苷键生成果糖或低聚果糖的水解酶.本研究在筛选菊粉酶高产菌株基础上,对产酶能力较强的1株湖北克鲁维酵母(Kluyvreromyces hubeiensis)的发酵产酶参数进行了优化;结果表明,该菌株在菊糖3%,蛋白胨3%,pH 5.0,30~37℃摇床培养48 h的产酶活力最高,其单位酶活比条件优化前提高了8~10倍. 展开更多
关键词 菊粉酶 湖北克鲁维酵母(K.hubeiensis) 3 5-二硝基水杨酸法
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Production of Xylanase and <i>β</i>-Xylosidase Enzymes by <i>Pseudozyma hubeiensis</i>in Solid State Fermentation
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作者 Nutan Mhetras Vidhyashri Mapare Digambar Gokhale 《Advances in Microbiology》 2019年第5期467-478,共12页
Xylanase is an important enzyme with potential application in the degradation of xylan component in the lignocellulosic biomass. There are very few reports on the production of cellulase free xylanases especially by y... Xylanase is an important enzyme with potential application in the degradation of xylan component in the lignocellulosic biomass. There are very few reports on the production of cellulase free xylanases especially by yeast strains which have great potential in paper and pulp industry in removing the hemicellulose from the treated or untreated pulp. In this study, P. hubeiensis NCIM 3574 isolated in our laboratory produced significant levels of extracellular cellulase free xylanase (2480 IU/g DSS) in solid state fermentation (SSF) using wheat bran and xylan. It also produced high levels of β-xylosidase (198 IU/g DSS) when grown in SSF using ground nut oil cake and xylan. These highest activities were obtained when fermented Koji was extracted with 1% NaCl supplemented with 0.5% of Triton X-100. These are the highest activities reported so far from yeast strains in the available literature. The crude xylanase preparation of P. hubeiensis produced xylooligosaccharides (XOS) without xylose proving its potential for XOS production with no further requirement of downstream processing. The XOS as prebiotic show beneficial effect on gut microflora such as Lactobacilli and Bifidobacteria which suppress the activity of pathogenic organisms. This xylanase also has a potential application as a bio-bleaching agent in paper and pulp industry. 展开更多
关键词 PSEUDOZYMA hubeiensis Cellulase-Free XYLANASE β-Xylosidase Solid State FERMENTATION Submerged FERMENTATION
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湖北元古代微体化石新材料 被引量:1
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作者 李酉兴 张素兴 《古生物学报》 CAS CSCD 北大核心 2006年第1期102-107,共6页
黄麦岭磷矿的8个中元古代黄麦岭组变质岩地层剖面中发现了两类微体化石。一类是丁丁虫中的Funnelasinensisgen.etsp.nov.,另一类是球形分类位置未定的Sinosphaera hubeiensisgen.etsp.nov.。两者均为多细胞化石,它们都含磷。在该磷矿中... 黄麦岭磷矿的8个中元古代黄麦岭组变质岩地层剖面中发现了两类微体化石。一类是丁丁虫中的Funnelasinensisgen.etsp.nov.,另一类是球形分类位置未定的Sinosphaera hubeiensisgen.etsp.nov.。两者均为多细胞化石,它们都含磷。在该磷矿中,凡是含微体化石越多的层位,磷矿的品位也就越高。 展开更多
关键词 Funnela SINENSIS gen.et sp.nov. Sinosphaera hubeiensis gen.et sp.nov. 中元古代 黄麦岭磷矿 湖北
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A NEW SPECIES OF ZYGNEMOPSIS(ZYGNEMATACEAE)AND ITS REPRODUCTION CYCLES
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作者 魏印心 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 1994年第2期180-184,192,共6页
The morphology and reproduction cycles of a new species,Zygnemopsis hubeiensis Wei are describedand illustrated for a culture homothallic strain collected from Wuhan,Hubei,China.The asexual reproduction is by partheno... The morphology and reproduction cycles of a new species,Zygnemopsis hubeiensis Wei are describedand illustrated for a culture homothallic strain collected from Wuhan,Hubei,China.The asexual reproduction is by parthenospores,aplanospores and akinetes.The major events of the sexual cycle,including the genuflection of reproductive cells,gametangial development,gamete formation,gamete fusion,zygote formation,zygospore development,and zygospore germination are observed. 展开更多
关键词 Zygnemopsis hubeiensis REPRODUCTION cycles isogamous HOMOTHALLISM
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贵州剑河寒武系“清虚洞组”高肌虫类清泉虫Qingquanella Cui et Huo,1985的初步研究
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作者 杨光福 赵元龙 +2 位作者 陈婉怡 张源 陈争鹏 《微体古生物学报》 CSCD 北大核心 2018年第3期302-308,共7页
本文描述了贵州剑河八郎寒武系第二统第四阶"清虚洞组"剑河生物群的高肌虫化石。化石壳体呈圆形,背边平直,前、后背角均钝,壳前背部具有"V"形槽。符合Qingquanella Cui et Huo,1985的属征,与模式种湖北清泉虫Q.hube... 本文描述了贵州剑河八郎寒武系第二统第四阶"清虚洞组"剑河生物群的高肌虫化石。化石壳体呈圆形,背边平直,前、后背角均钝,壳前背部具有"V"形槽。符合Qingquanella Cui et Huo,1985的属征,与模式种湖北清泉虫Q.hubeiensis相似,将其厘定为湖北清泉虫相似种Q.cf.hubeiensis。本属产于湖北水井沱组、石牌组及陕西辛集组,黔东地区"清虚洞组"发现清泉虫Qingquanella,增加了清泉虫的产地信息、新的类群,其壳体形态由切卵变圆形,生态环境由浅水向深水变化,为高肌虫的形态分类、早期演化与辐射分异提供新的材料。 展开更多
关键词 高肌虫 Qingquanella cf. hubeiensis 分类 “清虚洞组” 寒武系 贵州
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