Cu,Zn SOD is a highly conserved enzyme and the controversy about its evolutionary possibility in the near future has been lively. In order to further our understanding of the future fate of human Cu,Zn SOD, we adopt...Cu,Zn SOD is a highly conserved enzyme and the controversy about its evolutionary possibility in the near future has been lively. In order to further our understanding of the future fate of human Cu,Zn SOD, we adopted a strategy relating to the directed evolution to study how the mutants of human Cu,Zn SOD respond to different oxidative stress. After five rounds of screening, we found a mutant that can survive under harsh pressures and DNA sequencing proves that it shows a mutation responsible for the phenomenon. However, under natural pressure, our screening comes to nothing. Then we may draw the following conclusions: the evolution of biological macromolecules in some respect depends on their surroundings and if they are too familiar with a certain environment, they may embody evolutionary inertia.展开更多
Adsorption behaviors of Cu2Zn2SOD on mercury electrodes were studied by the electrostatic capillary curve and the relationship between polarographic ultimate current and height of mercury column in the absence of the ...Adsorption behaviors of Cu2Zn2SOD on mercury electrodes were studied by the electrostatic capillary curve and the relationship between polarographic ultimate current and height of mercury column in the absence of the mediators. The effects of impure and denatured SOD on cyclic voltammogram were investigated, confirming that no denaturation was caused during adsorption on mercury electrodes. Double potential step chronocoulometry has confirmed that SOD adsorption on mercury electrode to be the absorption of monomolecular layer.展开更多
文摘Cu,Zn SOD is a highly conserved enzyme and the controversy about its evolutionary possibility in the near future has been lively. In order to further our understanding of the future fate of human Cu,Zn SOD, we adopted a strategy relating to the directed evolution to study how the mutants of human Cu,Zn SOD respond to different oxidative stress. After five rounds of screening, we found a mutant that can survive under harsh pressures and DNA sequencing proves that it shows a mutation responsible for the phenomenon. However, under natural pressure, our screening comes to nothing. Then we may draw the following conclusions: the evolution of biological macromolecules in some respect depends on their surroundings and if they are too familiar with a certain environment, they may embody evolutionary inertia.
文摘Adsorption behaviors of Cu2Zn2SOD on mercury electrodes were studied by the electrostatic capillary curve and the relationship between polarographic ultimate current and height of mercury column in the absence of the mediators. The effects of impure and denatured SOD on cyclic voltammogram were investigated, confirming that no denaturation was caused during adsorption on mercury electrodes. Double potential step chronocoulometry has confirmed that SOD adsorption on mercury electrode to be the absorption of monomolecular layer.
