ECV304 was reported first in 1990 as a spont aneously-transformed and immortalized cell line derived from a Japanese HUVEC. S ubsequently, many studies validated that the ECV304 is a permanent endothelial cell line. I...ECV304 was reported first in 1990 as a spont aneously-transformed and immortalized cell line derived from a Japanese HUVEC. S ubsequently, many studies validated that the ECV304 is a permanent endothelial cell line. It has been used widely as an endothelial cell model and an useful re search tool in biomedicine and pharmacology. However, several distinct differenc es exist between ECV304 and HUVEC. Some studies even pointed out that ECV304 is not of HUVEC origin. According to the research data including ours, this reporte dly endothelial-derived permanent human cell line ECV304 may be dedifferentiated towards an epithelial phenotype. It is therefore not an appropriate cell line t o study endothelial cell biology. But cultured ECV304 cells can still be used as a model, tool or target in the pathophysiological and pharmacological studies, depending on whether or not their functional expression or markers are suitable for the research work.展开更多
背景与目的:血管性血友病因子(von Willebr and factor,vWF)、整合素β3(integrinβ3)与肿瘤转移的机制目前研究不清楚。本研究目的是观察vWF与integrinβ3在人肺癌细胞H460中的表达,人肺癌细胞H460与vWF黏附的关系,两因子与肿瘤轻移的...背景与目的:血管性血友病因子(von Willebr and factor,vWF)、整合素β3(integrinβ3)与肿瘤转移的机制目前研究不清楚。本研究目的是观察vWF与integrinβ3在人肺癌细胞H460中的表达,人肺癌细胞H460与vWF黏附的关系,两因子与肿瘤轻移的相关性及vWF与integrinβ3的相互关系。方法:①应用免疫组化技术观察vWF及integrinβ3在人肺癌细胞株H460的表达;②联合应用vWF黏附实验、抗体抑制实验及MTT法,观察vWF及integrinβ3与人肺癌细胞H460的黏附,及vWF与integrinβ3的相互关系。结果:①免疫组化结果显示H460细胞均表达vWF及integrinβ3。②vWF可以与H460细胞黏附,Anti-integrinβ3抑制后这种黏附降低,A值由1.59±0.06降到0.55±0.03,(P=0.01619),Anti-vWF抑制后这种黏附也降低,A值由1.60±0.06降到0.54±0.03,(P=0.01598),两者抑制黏附效果相同。结论:H460细胞表达vWF及integrinβ3,vWF可以与H460细胞黏附,vWF可能通过与integrinβ3结合发挥黏附作用,从而促进肿瘤细胞转移。展开更多
文摘ECV304 was reported first in 1990 as a spont aneously-transformed and immortalized cell line derived from a Japanese HUVEC. S ubsequently, many studies validated that the ECV304 is a permanent endothelial cell line. It has been used widely as an endothelial cell model and an useful re search tool in biomedicine and pharmacology. However, several distinct differenc es exist between ECV304 and HUVEC. Some studies even pointed out that ECV304 is not of HUVEC origin. According to the research data including ours, this reporte dly endothelial-derived permanent human cell line ECV304 may be dedifferentiated towards an epithelial phenotype. It is therefore not an appropriate cell line t o study endothelial cell biology. But cultured ECV304 cells can still be used as a model, tool or target in the pathophysiological and pharmacological studies, depending on whether or not their functional expression or markers are suitable for the research work.
文摘背景与目的:血管性血友病因子(von Willebr and factor,vWF)、整合素β3(integrinβ3)与肿瘤转移的机制目前研究不清楚。本研究目的是观察vWF与integrinβ3在人肺癌细胞H460中的表达,人肺癌细胞H460与vWF黏附的关系,两因子与肿瘤轻移的相关性及vWF与integrinβ3的相互关系。方法:①应用免疫组化技术观察vWF及integrinβ3在人肺癌细胞株H460的表达;②联合应用vWF黏附实验、抗体抑制实验及MTT法,观察vWF及integrinβ3与人肺癌细胞H460的黏附,及vWF与integrinβ3的相互关系。结果:①免疫组化结果显示H460细胞均表达vWF及integrinβ3。②vWF可以与H460细胞黏附,Anti-integrinβ3抑制后这种黏附降低,A值由1.59±0.06降到0.55±0.03,(P=0.01619),Anti-vWF抑制后这种黏附也降低,A值由1.60±0.06降到0.54±0.03,(P=0.01598),两者抑制黏附效果相同。结论:H460细胞表达vWF及integrinβ3,vWF可以与H460细胞黏附,vWF可能通过与integrinβ3结合发挥黏附作用,从而促进肿瘤细胞转移。
