Objective:To investigate the effect ofα1-antitrypsin combined with bone marrow mesenchymal stem cells on retinopathy in diabetic rats and its mechanism.Methods:A model of diabetic retinopathy was established by intra...Objective:To investigate the effect ofα1-antitrypsin combined with bone marrow mesenchymal stem cells on retinopathy in diabetic rats and its mechanism.Methods:A model of diabetic retinopathy was established by intraperitoneal injection of streptozotocin.The 30 Wistar rats successfully modeled were randomly divided into a model group,a bone marrow mesenchymal stem cell group and a combined group(α1-antitrypsin combined with bone marrow Mesenchymal stem cells),the blood glucose and serum insulin levels of diabetic rats were measured 4 weeks after treatment.Enzyme-linked immunosorbent assay(ELISA)for measuring serum inflammatory factors IL-1β,IL-6 and TNF-α in rats.Observing the pathological morphology of rat retina under hematoxylin-eosin staining(HE).TUNEL staining to observe the apoptosis of rat retinal nerve cells.Immunohistochemical method to detect the expression level of CD45 in retinal tissue.Real-time fluorescence quantitative PCR was used to detect the expression of retinal vascular endothelial growth factor(VEGF),hypoxiainducible factor-1α(HIF-1α),and angiotensinⅡ(ANGⅡ)mRNA.Western blot was used to detect the expression of p38 MAPK/NF-κB signaling pathway-related proteins in the retinal tissue of each group of rats.Results:Compared with the control group,the rats in the model group had increased blood glucose,decreased insulin levels,increased serum IL-1β,IL-6,and TNF-α levels,and had obvious lesions in the retina.CD45 showed high expression in retinal tissue,VEGF,HIF-1α,ANGⅡ mRNA expression increased,p-p38,p-p65,p-IκBα protein expression increased(P<0.05).Compared with the model group,the bone marrow mesenchymal stem cell group and the combined group have decreased blood glucose,increased insulin levels,and decreased serum IL-1β,IL-6 and TNF-α levels.Retinopathy is improved,apoptosis of retinal nerve cells is reduced,CD45 expression in retinal tissue is reduced,VEGF,HIF-1α,ANGⅡ mRNA expression is decreased,and p-p38,p-p65,p-IκBα protein expression is decreased.Compared with the bone marrow mesenchymal stem cell group,the effect of the combined group was more obvious(P<0.05).Conclusion:α1-antitrypsin combined with bone marrow mesenchymal stem cell transplantation can improve the degree of retinopathy in diabetic rats.The mechanism may be related to the inhibition of p38 MAPK/NF-κB signaling pathway.展开更多
目的 血小板-内皮细胞粘附分子-1(platelet endothelial cell adhesion molecule-1,PECAM-1)、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、可溶性骨髓细胞样转录因子-1(soluble bone marrow cell-like transcription fac...目的 血小板-内皮细胞粘附分子-1(platelet endothelial cell adhesion molecule-1,PECAM-1)、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、可溶性骨髓细胞样转录因子-1(soluble bone marrow cell-like transcription factor-1,sTLT-1)在急性脑梗死(acute cerebral infarction,ACI)患者中的表达及相关性研究。方法 选取2019年5月-2020年10月我院收治的ACI患者77例作为实验组,按照神经功能缺损评分量表(national institute of health stroke scale,NIHSS)评分分为轻度患者36例,中度患者25例,重度患者16例;根据磁共振成像(magnetic resonance imaging,MRI)反应的梗死面积将患者分为小面积梗死灶(small area of infarction,SI)38例、中度面积梗死灶(moderate area of infarct,MI)26例以及大面积梗死灶(large area of infarction,LI)13例,同时选取在我院同一时间点进行健康体检的健康人员77例作为正常组,采用酶联免疫吸附法检测PECAM-1、GFAP、sTLT-1水平。结果 与正常组相比,实验组PECAM-1、GFAP、sTLT-1显著升高,具有统计学差异(P<0.05);与轻度组相比,中度组和重度组水平升高,与中度组相比,重度组水平显著升高,具有统计学差异(P<0.05);与SI相比,Ml和LI水平升高,与MI相比,LI水平显著升高,具有统计学差异(P<0.05)。结论 PECAM-1、GFAP、sTLT-1在ACI患者血清中表现异常,与患者的严重程度、梗死面积有密切关系,可能参与ACI的发生发展,提示PECAM-1、GFAP、sTLT-1在ACI的临床诊断中具有一定作用。展开更多
基金Key Rearch and Development projects in Shaanxi Province(2019SF-084).
文摘Objective:To investigate the effect ofα1-antitrypsin combined with bone marrow mesenchymal stem cells on retinopathy in diabetic rats and its mechanism.Methods:A model of diabetic retinopathy was established by intraperitoneal injection of streptozotocin.The 30 Wistar rats successfully modeled were randomly divided into a model group,a bone marrow mesenchymal stem cell group and a combined group(α1-antitrypsin combined with bone marrow Mesenchymal stem cells),the blood glucose and serum insulin levels of diabetic rats were measured 4 weeks after treatment.Enzyme-linked immunosorbent assay(ELISA)for measuring serum inflammatory factors IL-1β,IL-6 and TNF-α in rats.Observing the pathological morphology of rat retina under hematoxylin-eosin staining(HE).TUNEL staining to observe the apoptosis of rat retinal nerve cells.Immunohistochemical method to detect the expression level of CD45 in retinal tissue.Real-time fluorescence quantitative PCR was used to detect the expression of retinal vascular endothelial growth factor(VEGF),hypoxiainducible factor-1α(HIF-1α),and angiotensinⅡ(ANGⅡ)mRNA.Western blot was used to detect the expression of p38 MAPK/NF-κB signaling pathway-related proteins in the retinal tissue of each group of rats.Results:Compared with the control group,the rats in the model group had increased blood glucose,decreased insulin levels,increased serum IL-1β,IL-6,and TNF-α levels,and had obvious lesions in the retina.CD45 showed high expression in retinal tissue,VEGF,HIF-1α,ANGⅡ mRNA expression increased,p-p38,p-p65,p-IκBα protein expression increased(P<0.05).Compared with the model group,the bone marrow mesenchymal stem cell group and the combined group have decreased blood glucose,increased insulin levels,and decreased serum IL-1β,IL-6 and TNF-α levels.Retinopathy is improved,apoptosis of retinal nerve cells is reduced,CD45 expression in retinal tissue is reduced,VEGF,HIF-1α,ANGⅡ mRNA expression is decreased,and p-p38,p-p65,p-IκBα protein expression is decreased.Compared with the bone marrow mesenchymal stem cell group,the effect of the combined group was more obvious(P<0.05).Conclusion:α1-antitrypsin combined with bone marrow mesenchymal stem cell transplantation can improve the degree of retinopathy in diabetic rats.The mechanism may be related to the inhibition of p38 MAPK/NF-κB signaling pathway.
文摘目的 血小板-内皮细胞粘附分子-1(platelet endothelial cell adhesion molecule-1,PECAM-1)、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、可溶性骨髓细胞样转录因子-1(soluble bone marrow cell-like transcription factor-1,sTLT-1)在急性脑梗死(acute cerebral infarction,ACI)患者中的表达及相关性研究。方法 选取2019年5月-2020年10月我院收治的ACI患者77例作为实验组,按照神经功能缺损评分量表(national institute of health stroke scale,NIHSS)评分分为轻度患者36例,中度患者25例,重度患者16例;根据磁共振成像(magnetic resonance imaging,MRI)反应的梗死面积将患者分为小面积梗死灶(small area of infarction,SI)38例、中度面积梗死灶(moderate area of infarct,MI)26例以及大面积梗死灶(large area of infarction,LI)13例,同时选取在我院同一时间点进行健康体检的健康人员77例作为正常组,采用酶联免疫吸附法检测PECAM-1、GFAP、sTLT-1水平。结果 与正常组相比,实验组PECAM-1、GFAP、sTLT-1显著升高,具有统计学差异(P<0.05);与轻度组相比,中度组和重度组水平升高,与中度组相比,重度组水平显著升高,具有统计学差异(P<0.05);与SI相比,Ml和LI水平升高,与MI相比,LI水平显著升高,具有统计学差异(P<0.05)。结论 PECAM-1、GFAP、sTLT-1在ACI患者血清中表现异常,与患者的严重程度、梗死面积有密切关系,可能参与ACI的发生发展,提示PECAM-1、GFAP、sTLT-1在ACI的临床诊断中具有一定作用。
