Chemical synthesis and cloning of human calcitonin (hCT) cDNA

Chemical synthesis and cloning of human calcitonin (hCT) cDNA. Methods and Results:The cDNA sequence coding for human calcitonin (hCT) was divided into 6 fragments, synthesized by ABI 391 DNA synthesis instrument and cloned into PGEM7Z(+) vector following phosphorylation, annealing and ligation of the 6 fragments. The sequence of cloned hCT cDNA was proved to be the same as designed by double DNA sequencing. Conclusion: A reliable and forthright method of chemical synthesis and cloning of oligo-peplides cDNA (or gene) was provided in this study. This paper is a basic work for expression of hCT cDNA.

人降钙素cDNA的化学合成与克隆

目的：克隆人降钙素ｃＤＮＡ。方法和结果：应用ＡＢＩ３９１ＤＮＡ合成仪，将人降钙素ｃＤＮＡ分成６个片段合成。退火、磷酸化、连接，最终将人降钙素ｃＤＮＡ克隆于载体ｐＧＥＭ７ｚ（＋），经ＤＮＡ双链测序，证明克隆到的人降钙素ｃＤＮＡ序列与设计的完全一致。结论：本研究为寡肽ｃＤＮＡ（或基因）的化学合成与克隆提供了简捷、可靠的方法；为人的降钙素ｃＤＮＡ的高效表达奠定了基础。