In this paper, the elastic modulus measuring of human cementum is studied. The average value of the elastic modulus measured from 100 specimens is E = 2.398 +/- 0.455 GPa. The specimens were made from incisors, canine...In this paper, the elastic modulus measuring of human cementum is studied. The average value of the elastic modulus measured from 100 specimens is E = 2.398 +/- 0.455 GPa. The specimens were made from incisors, canines, bicuspids, and molars. The testing was done on type AG-10TA electronic-mechanical universal material testing machine. It was verified by mathematical statistics that the probability distribution of measured data obeys normal distribution. By the great difference in elastic moduli between cementum and dentin it is explained that 1. why more cementum grows at the root apexes; 2. the behavior of cementum must be taken into consideration when the stress analysis for teeth is carried out.展开更多
目的探讨提取的牙骨质非胶原蛋白(cementum noncollagenous proteins,CNCPs)对乳牙牙髓干细胞(Stem cell from human exfoliated deciduous teeth,SHED)增殖、分化和功能的影响。方法拔除2岁龄牛健康牙,提取牙骨质基质的盐酸胍提取物。...目的探讨提取的牙骨质非胶原蛋白(cementum noncollagenous proteins,CNCPs)对乳牙牙髓干细胞(Stem cell from human exfoliated deciduous teeth,SHED)增殖、分化和功能的影响。方法拔除2岁龄牛健康牙,提取牙骨质基质的盐酸胍提取物。采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析盐酸胍提取物的组分和分子量。采用MTT、ALP染色、茜素红染色和RT-PCR等方法观察牙骨质非胶原蛋白对SHED的影响。结果 CNCPs对SHED的增殖无促进作用,CNCPs(1ug/mL)组作用于SHED,在7d和14d时,同不含CNCPs组相比,能明显上调SHED的碱性磷酸酶活性(P<0.01),CNCPs(1ug/mL)可明显促进SHED表达DSPP、DMP1、BSP和Runx-2(P<0.05)及矿化。结论 CNCPs能够促进SHED向成牙本质细胞的分化及矿化。展开更多
文摘In this paper, the elastic modulus measuring of human cementum is studied. The average value of the elastic modulus measured from 100 specimens is E = 2.398 +/- 0.455 GPa. The specimens were made from incisors, canines, bicuspids, and molars. The testing was done on type AG-10TA electronic-mechanical universal material testing machine. It was verified by mathematical statistics that the probability distribution of measured data obeys normal distribution. By the great difference in elastic moduli between cementum and dentin it is explained that 1. why more cementum grows at the root apexes; 2. the behavior of cementum must be taken into consideration when the stress analysis for teeth is carried out.
文摘目的观察苯妥英钠(PHT)对内毒素作用下人牙周膜干细胞(h PDLSCs)在牙骨质片上附着的影响。方法本研究于2012年6月至2013年2月在滨州医学院中心实验室和西安交通大学医学院中心实验室完成。利用扫描电镜和透射电镜观察h PDLSCs分别在20μg/m L PHT+100 ng/m L脂多糖(LPS)与100 ng/m L LPS作用下在牙骨质片上的附着情况以及细胞超微结构的变化。结果与细胞培养液中加入100 ng/m L LPS比较,培养液中加入20μg/m L PHT+100 ng/m L LPS培养72 h后h PDLSCs胞浆内细胞器更丰富,粗面内质网扩张,高尔基复合体发达,有大量的线粒体和核糖体;培养第7 d和第21 d,细胞生长更密集,伪足较多且粗大,与牙骨质片的附着更紧密,细胞周围钙盐沉积以及胶原蛋白合成更加活跃。结论 PHT可促进h PDLSCs在牙骨片上的附着和生长。
