Background and objective The expression of various trace elements and markers in lung cancer is controversial.The aim of this study is to evaluate the presence of calcium(Ca),copper(Cu),iron(Fe) and carcinoembryonic a...Background and objective The expression of various trace elements and markers in lung cancer is controversial.The aim of this study is to evaluate the presence of calcium(Ca),copper(Cu),iron(Fe) and carcinoembryonic antigen(CEA) in cancerous untreated lung tissues and to determine a possible association between these markers and lung cancer.Methods Fourty-eight cancerous lung tissue blocks,from Sultan Qaboos University Hospital,Sultanate of Oman,were studied.Fe,Ca,Cu,and CEA were demonstrated in the tissue blocks using Perl's Prussian blue,Von Kossa's,modified rhodanine and immunohistochemical staining methods,respectively.Results Twenty-three of 48 specimens showed positive Fe staining,2 showed positive Ca staining and Cu was absent in all specimens.93.7% expressed CEA in varying degree of positivity.81.25% of these sections showed high expression of CEA.Conclusion Tissue concentrations of trace elements were not elevated in lung cancer and therefore cannot be considered as a potential marker.Despite the low sensitivity and specificity of CEA as previously reported,tissue CEA should be considered as a potential marker in the evaluation of lung cancer.展开更多
In this study, we have demonstrated the conformational changes to DNA induced by abnormal interactions of copper using circular dichroism, in combination with UV-absorbance and fluorescence spectroscopy. Results confi...In this study, we have demonstrated the conformational changes to DNA induced by abnormal interactions of copper using circular dichroism, in combination with UV-absorbance and fluorescence spectroscopy. Results confirm that binding of copper to bases of DNA in chromatin is concentration dependent. Binding efficiency of Cu2~ ions to DNA is increased in proportion to the degree of unwinding of the double helix induced by denaturation. Altered B-DNA conformation will alter the integrity of DNA which may affect the normal process of DNA replication and transcription. Copper induced DNA damage in the brain may cause neurotoxicity and the neuronal cell death and is implicated in Alzheimer＇s disease and other neurological disorders.展开更多
A new method of growing human embryonic neurons in glass capillary is proposed. Beside the simplicity of the method, the main advantages are represented by the possibility to study the interactions between a direction...A new method of growing human embryonic neurons in glass capillary is proposed. Beside the simplicity of the method, the main advantages are represented by the possibility to study the interactions between a directional flow of human motile cells (leukocytes, red blood cells, sperm cells) and the neuronal monolayer and to evaluate the toxicity on neuronal growth of a copper wire or others filiform materials introduced in the glass capillary. The neurotoxicity of copper is verified by this device, because copper wire inserted into the capillary interferes with engraftment and growth of neuronal cells. No interference by neuronal monolayer was found with the flow of human sperms trough the capillary. Also the flow of leukocytes and erythrocytes occurred without agglutination or adhesion to cellular monolayer with serial observation at 30, 60, 120 minutes. A further advantage of this method is the possibility of withdrawing culture medium microquantities from capillary in order to study neuronal secretory activity.展开更多
本文通过张掖西城驿遗址文化层沉积物及现代表土沉积物元素地球化学分析,结合高分辨率碳化植物种子14C测年和最新考古发掘资料,揭示了西城驿遗址4200-3500 BP cal期间人类活动特征,佐证了不同文化阶段人类冶金活动强度变化。Rb/Sr比值...本文通过张掖西城驿遗址文化层沉积物及现代表土沉积物元素地球化学分析,结合高分辨率碳化植物种子14C测年和最新考古发掘资料,揭示了西城驿遗址4200-3500 BP cal期间人类活动特征,佐证了不同文化阶段人类冶金活动强度变化。Rb/Sr比值、磁化率和重金属元素(Cu、Zn、Pb、As、Ni)含量变化过程显示,西城驿地区4200-4000 BP cal马厂文化时期人类活动增强,冶金活动开始出现;4000-3700 BP cal西城驿文化时期出现了冶金高峰期;四坝文化时期(3700-3500 BP cal)冶金活动强度相对减弱。展开更多
文摘Background and objective The expression of various trace elements and markers in lung cancer is controversial.The aim of this study is to evaluate the presence of calcium(Ca),copper(Cu),iron(Fe) and carcinoembryonic antigen(CEA) in cancerous untreated lung tissues and to determine a possible association between these markers and lung cancer.Methods Fourty-eight cancerous lung tissue blocks,from Sultan Qaboos University Hospital,Sultanate of Oman,were studied.Fe,Ca,Cu,and CEA were demonstrated in the tissue blocks using Perl's Prussian blue,Von Kossa's,modified rhodanine and immunohistochemical staining methods,respectively.Results Twenty-three of 48 specimens showed positive Fe staining,2 showed positive Ca staining and Cu was absent in all specimens.93.7% expressed CEA in varying degree of positivity.81.25% of these sections showed high expression of CEA.Conclusion Tissue concentrations of trace elements were not elevated in lung cancer and therefore cannot be considered as a potential marker.Despite the low sensitivity and specificity of CEA as previously reported,tissue CEA should be considered as a potential marker in the evaluation of lung cancer.
文摘In this study, we have demonstrated the conformational changes to DNA induced by abnormal interactions of copper using circular dichroism, in combination with UV-absorbance and fluorescence spectroscopy. Results confirm that binding of copper to bases of DNA in chromatin is concentration dependent. Binding efficiency of Cu2~ ions to DNA is increased in proportion to the degree of unwinding of the double helix induced by denaturation. Altered B-DNA conformation will alter the integrity of DNA which may affect the normal process of DNA replication and transcription. Copper induced DNA damage in the brain may cause neurotoxicity and the neuronal cell death and is implicated in Alzheimer＇s disease and other neurological disorders.
文摘A new method of growing human embryonic neurons in glass capillary is proposed. Beside the simplicity of the method, the main advantages are represented by the possibility to study the interactions between a directional flow of human motile cells (leukocytes, red blood cells, sperm cells) and the neuronal monolayer and to evaluate the toxicity on neuronal growth of a copper wire or others filiform materials introduced in the glass capillary. The neurotoxicity of copper is verified by this device, because copper wire inserted into the capillary interferes with engraftment and growth of neuronal cells. No interference by neuronal monolayer was found with the flow of human sperms trough the capillary. Also the flow of leukocytes and erythrocytes occurred without agglutination or adhesion to cellular monolayer with serial observation at 30, 60, 120 minutes. A further advantage of this method is the possibility of withdrawing culture medium microquantities from capillary in order to study neuronal secretory activity.
文摘本文通过张掖西城驿遗址文化层沉积物及现代表土沉积物元素地球化学分析,结合高分辨率碳化植物种子14C测年和最新考古发掘资料,揭示了西城驿遗址4200-3500 BP cal期间人类活动特征,佐证了不同文化阶段人类冶金活动强度变化。Rb/Sr比值、磁化率和重金属元素(Cu、Zn、Pb、As、Ni)含量变化过程显示,西城驿地区4200-4000 BP cal马厂文化时期人类活动增强,冶金活动开始出现;4000-3700 BP cal西城驿文化时期出现了冶金高峰期;四坝文化时期(3700-3500 BP cal)冶金活动强度相对减弱。
