The Main Characteristics of China’s International Human Rights Cooperation and Exchanges from 2010 to 2020

China’s international human rights cooperation and exchanges from 2010 to 2020 have the following main characteristics: China’s cooperation with United Nations multilateral human rights institutions is the main part of China’s international human rights cooperation and exchanges China has been deeply involved in cooperation with UN multilateral human rights agencies and is playing an increasingly important role in them Bilateral human rights dialogues are the main focus of China’s bilateral human rights cooperation and exchanges, and in recent years the objects of the human rights dialogue have been expanding In addition to the official level of multilateral and bilateral human rights cooperation and exchanges, the Chinese government also encourages human rights organizations to actively participate in the international human rights exchanges and makes those organizations play an increasingly important role in international human rights exchanges The Chinese human rights organizations also develop innovative ways for international human rights exchanges and become an important channel for the international community to understand the ideas and situation of human rights protection in China.

Empirical Human Rights Studies Contribute to Building a Society Under the Rule of Law——Summary of the Fifth National Meeting for the Exchange of Work Experience Among Human Rights Research Institutions

From Nov. 5 to 6, 2013, the China Society for Human Rights Studies （CSHRS）held the Fifth National Meeting for the Exchange of Work Experience Among Human Rights Research Institutions at Wuhan University. More than 100 experts and scholars, focusing on empirical human rights studies and their contribution to building a society based on the rule of law, shared their work experience and discussed effective strategies. CSHRS President Luo Haocai, who served as vice chairman of the 10th National Committee of the Chinese People＇s Political Consultative Conference,

Human Rights Consensus and the Common Prosperity of Civilizations--A Summary of Views at the “2018·China-Europe Seminar on Human Rights”

The "2018·China-Europe Seminar on Human Rights" was successfully held at the College of Europe in Bruges, Belgium, on June 28. The seminar was hosted by the China Society for Human Rights Studies and the International Institute of Human Rights, and was organized by the Human Rights Institute at the Southwest University of Political Science and Law and the French ADELIE Language and Cultural Exchange Association. The University of Strasbourg, Vrije Universiteit Brussel and European Institute for Political and Strategic Communication were served as co-organizers. Over 60 experts and scholars in the field of human rights from China and Europe attended the seminar in search of the diversity of civilization and human rights protection, focusing on themes such as "the philosophy of human rights in a Chinese context", "the philosophy of human rights in the Universal Declaration of Human Rights and its reflection", "human rights theory and practice in a pluralistic world", "human rights exchanges and dialogues under the diversity of civilization", and "in search of human rights consensus in diverse civilizations." In the context of respecting diversity, the seminar sought a consensus to promote trans-cultural communication in the form of dialogues and to strengthen understanding between the academic circles of China and Europe.

Establish an International Communication Platform for Human Rights and Promote China's Discourse Power on Human Rights——A Review on Ten Years of Beijing Forum on Human Rights

The beijing forum on Human Rights is a global human rights forum founded by the china Society for Human Rights Studies in 2008? it has been successfully held for nine times? in the past 10 years, the forum has kept up with the trend of the times and carefully set up issues; seeking common ground while reserving differences,seeking the greatest consensus on human rights; being open and inclusive, and building a model of international human rights exchange and cooperation? The forum has become a high-level academic platform for human rights exchanges and cooperation on the basis of equality,harmony, tolerance, openness and mutual respect, and has played an important role in promoting the healthy development of the international human rights cause? With the socialism with chinese characteristics entering a new era, the beijing forum on Human Rights needs to position new roles in the new era, seek more clear development momentum and direction, and promote the vitality, cohesiveness and international influence of the forum in a deep and all-round way and contribute to the development of world human rights cause and global human rights governance?

Generation of mouse anti-human urate anion exchanger antibody by genetic immunization and its identification

Background Human urate anion exchanger (hURAT1) as a major urate transporter expressed on renal tubular epithelial cells regulates blood urate level by reabsorbing uric acid. Antibody is an important tool to study hURAT1. This study aimed, by genetic immunization, to produce mouse anti-hURAT1 polyclonal antibody with high throughput and high specificity and to detect the location of hURAT1 in human kidney.Methods Human renal total RNA was isolated and the entire cDNA of hURAT1 was amplified by RT-PCR. The sequence of intracellular high antigenicity fragment (A280 to R349) was chosen by prediction software of protein antigenicity, and its cDNA was amplified from cDNA of hURAT1, and then cloned into pBQAP-TT vector to construct recombinant plasmid pBQAP-TT-hURAT1-210 for genetic immunization. Mice were inoculated with this recombinant plasmid and two other adjuvant plasmids, pCMVi-GMCSF and pCMVi-Flt3L, which helped to enhance the antibody’s generation. After four weeks, the mice were sacrificed to obtain the anti-hURAT1 antibody from serum. The antibody was identified by western blot analysis and immunohistochemistry. At the same time, rabbit anti-hURAT1 antibody was produced by protein immunization. The specificity and efficiency between the rabbit and mouse anti-hURAT1 antibody were compared by western blot analysis and immunohistochemistry. Results The entire cDNA of hURAT1 and cDNA of its intracellular high immunogenic fragment were amplified successfully. Recombinant plasmid pBQAP-TT-hURAT1-210 for genetic immunization was confirmed by restriction digestion and sequencing. Both!the mouse anti-hURAT1 antibody and rabbit anti-hURAT1 antibody recognized 58kD hURAT1 and 64kD glycosylated hURAT1 protein bands in western blot. Immunohistochemically, hURAT1 was located at the brush border membrane of renal proximal tubular cells. In addition, the throughput and specificity of the mouse anti-hURAT1 antibody were higher than those of the rabbit anti-hURAT1 antibody.Conclusion Genetic immunization can generate anti-hURAT1 polyclonal antibody of high throughput and specificity.