In order to reveal variation and revolution of NP genes of human avian H5 N1 influenza virus strains, the NP gene of a human avian H5 N1 influenza virus strain in Guangdong was sequenced and the global NP genes of str...In order to reveal variation and revolution of NP genes of human avian H5 N1 influenza virus strains, the NP gene of a human avian H5 N1 influenza virus strain in Guangdong was sequenced and the global NP genes of strains were retrieved. The sequences were analyzed by DNAStar 5.0, and the evolutionary speed was studied with reference to the epidemiological data. It was found that NP genes of 45 strains during 1997-2006 were homologically classified into three groups： strains in 1997-1998, strains in 2004-2005 and strains from 2003 to 2006. There were 35 substitutions in NPs in all strains accounting for a ratio of 7.03% （35/498）. An additional glycoprotein domain （NGT430-432） was found in NP genes in the strains of 2003-2006, the mutation of N370S in GD-01-06 resulted in occurrence of one more glycoprotein domain （NES368-370）. In the synonymous variation, Ks values in NP were 2.03 × 10^-5-2.55 × 10^-5 Nt/d and K. values in NP were 1.58 × 10^-6-3.10 × 10^-6 Nt/d. There didn＇t exist obviously selective pressure. An additional glycoprotein domain in every strain of 2003-2006 and one more in strain GD-01-06 might change the antigenicity of human avian H5 N1 influenza virus. The variation on human avian H5 N1 influenza strains occurred frequently in the natural world, which would result in high probability of human-human transmission along with the natural evolution of the virus.展开更多
Antibodies are ideal for controlling the influenza A virus,but their effect on newly emerging strains is unclear.Here,we assessed the neutralization activity of the humanized monoclonal antibodies(mAbs)F10,H98 and H40...Antibodies are ideal for controlling the influenza A virus,but their effect on newly emerging strains is unclear.Here,we assessed the neutralization activity of the humanized monoclonal antibodies(mAbs)F10,H98 and H40 against circulating influenza viruses(H5N1,H1N1,H3N2 and H7N7 and new subtypes viruses H5N6 and H7N9).The results showed that all the three humanized mAbs(F10,H98 and H40)displayed different degrees of virus neutralization activities when encountered with different subtypes of influenza viruses.Remarkably,the humanized monoclonal antibody F10 produced higher and broader neutralization titers(range 25–1.56μg/ml)than those of the other two humanized mAbs(H98(range 50–3.12μg/ml),H40(range 50–5.56μg/ml))to against the viruses H5N1,H1N1,H3N2,H7N7,H5N6 and H7N9.This mAb may represent a new class of heterosubtypic neutralizing humanized mAb that could replace vaccines and chemical drugs.展开更多
With the support by the National Natural Science Foundation of China,the research team led by Prof.Yu Hongjie(余宏杰)at the School of Public Health,Fudan University,Key Laboratory of Public Health Safety,Ministry of E...With the support by the National Natural Science Foundation of China,the research team led by Prof.Yu Hongjie(余宏杰)at the School of Public Health,Fudan University,Key Laboratory of Public Health Safety,Ministry of Education,and the Key Laboratory of Surveillance and Early Warning on Infectious Disease,Chinese Center for Disease Control and Prevention,has published the paper entitled“Global epi-展开更多
文摘In order to reveal variation and revolution of NP genes of human avian H5 N1 influenza virus strains, the NP gene of a human avian H5 N1 influenza virus strain in Guangdong was sequenced and the global NP genes of strains were retrieved. The sequences were analyzed by DNAStar 5.0, and the evolutionary speed was studied with reference to the epidemiological data. It was found that NP genes of 45 strains during 1997-2006 were homologically classified into three groups： strains in 1997-1998, strains in 2004-2005 and strains from 2003 to 2006. There were 35 substitutions in NPs in all strains accounting for a ratio of 7.03% （35/498）. An additional glycoprotein domain （NGT430-432） was found in NP genes in the strains of 2003-2006, the mutation of N370S in GD-01-06 resulted in occurrence of one more glycoprotein domain （NES368-370）. In the synonymous variation, Ks values in NP were 2.03 × 10^-5-2.55 × 10^-5 Nt/d and K. values in NP were 1.58 × 10^-6-3.10 × 10^-6 Nt/d. There didn＇t exist obviously selective pressure. An additional glycoprotein domain in every strain of 2003-2006 and one more in strain GD-01-06 might change the antigenicity of human avian H5 N1 influenza virus. The variation on human avian H5 N1 influenza strains occurred frequently in the natural world, which would result in high probability of human-human transmission along with the natural evolution of the virus.
基金This work was supported by the National Key Research and Development Program of China(2017YFD0501705 and 2016YFD0500203).
文摘Antibodies are ideal for controlling the influenza A virus,but their effect on newly emerging strains is unclear.Here,we assessed the neutralization activity of the humanized monoclonal antibodies(mAbs)F10,H98 and H40 against circulating influenza viruses(H5N1,H1N1,H3N2 and H7N7 and new subtypes viruses H5N6 and H7N9).The results showed that all the three humanized mAbs(F10,H98 and H40)displayed different degrees of virus neutralization activities when encountered with different subtypes of influenza viruses.Remarkably,the humanized monoclonal antibody F10 produced higher and broader neutralization titers(range 25–1.56μg/ml)than those of the other two humanized mAbs(H98(range 50–3.12μg/ml),H40(range 50–5.56μg/ml))to against the viruses H5N1,H1N1,H3N2,H7N7,H5N6 and H7N9.This mAb may represent a new class of heterosubtypic neutralizing humanized mAb that could replace vaccines and chemical drugs.
文摘With the support by the National Natural Science Foundation of China,the research team led by Prof.Yu Hongjie(余宏杰)at the School of Public Health,Fudan University,Key Laboratory of Public Health Safety,Ministry of Education,and the Key Laboratory of Surveillance and Early Warning on Infectious Disease,Chinese Center for Disease Control and Prevention,has published the paper entitled“Global epi-
