Human milk fat(HMF)is an important source of nutrients and energy for infants.Triacylglycerols(TAGs)account for about 98%of HMF and have a unique molecular structure.HMF is highly enriched in palmitic acid(PA)at the s...Human milk fat(HMF)is an important source of nutrients and energy for infants.Triacylglycerols(TAGs)account for about 98%of HMF and have a unique molecular structure.HMF is highly enriched in palmitic acid(PA)at the sn-2 position of the glycerol backbone(more than 70%)and in unsaturated fatty acids at the sn-1,3 position.The specific TAG structure in HMF plays a valuable function in infant growth.Sn-2 palmitate(mainly 1,3-dioleoyl-2-palmitoyl-glycerol)is one of the structured TAGs that is commonly supplemented into infant formula in order to enable it to present a similar structure to HMF.In this review,the development of the lipase-catalyzed synthesis of sn-2 palmitate over the last 25 years are summarized,with a focus on reaction schemes in a laboratory setting.Particular attention is also paid to the commercialized sn-1,3 regioselective lipases that are used in structured TAGs synthesis,to general methods of TAG analysis,and to successfully developed sn-2 palmitate products on the market.Prospects for the lipase-catalyzed synthesis of sn-2 palmitate are discussed.展开更多
Human milk fat substitute is an important structured lipid intended for infant formula. This present study compared human milk fat substitute from R. opacus with commercial product and evaluated whether it is qualifie...Human milk fat substitute is an important structured lipid intended for infant formula. This present study compared human milk fat substitute from R. opacus with commercial product and evaluated whether it is qualified for infant formula. It was proven that both human milk fat substitute and commercial product were mainly composed of C16:0 and C18:1 in total fatty acid composition. Human milk fat substitute contained high level of C16:0 at sn -2 position (54.57%) and high level of 1,3-dioleoyl-2-palmitoylglycerol (37.94%), which was in accordance with human milk fat and commercial product. In addition, relative high level of odd chain fatty acids (1.56%) and C16:1 (7.27%) was identified, which made human milk fat substitute unique. Human milk fat substitute was short of medium-chain fatty acids, such as C8:0, C10:0 and polyunsaturated fatty acids, such as, eicosapentaenoic acid and docosahexaenoic acid. In conclusion, human milk fat substitute from R. opacus was qualified as a component for infant formula in term of fatty acid composition and triacylglycerol.展开更多
基金supported by a National Natural Science Foundation of China grant(31701558)the Young Elite Scientists Sponsorship Program by CAST(2017QNRC001)+1 种基金the Overseas Expertise Introduction Project for Discipline Innovation(111 Project,B90719028)the national first-class discipline program of Food Science and Technology(JUFSTR20180202).
文摘Human milk fat(HMF)is an important source of nutrients and energy for infants.Triacylglycerols(TAGs)account for about 98%of HMF and have a unique molecular structure.HMF is highly enriched in palmitic acid(PA)at the sn-2 position of the glycerol backbone(more than 70%)and in unsaturated fatty acids at the sn-1,3 position.The specific TAG structure in HMF plays a valuable function in infant growth.Sn-2 palmitate(mainly 1,3-dioleoyl-2-palmitoyl-glycerol)is one of the structured TAGs that is commonly supplemented into infant formula in order to enable it to present a similar structure to HMF.In this review,the development of the lipase-catalyzed synthesis of sn-2 palmitate over the last 25 years are summarized,with a focus on reaction schemes in a laboratory setting.Particular attention is also paid to the commercialized sn-1,3 regioselective lipases that are used in structured TAGs synthesis,to general methods of TAG analysis,and to successfully developed sn-2 palmitate products on the market.Prospects for the lipase-catalyzed synthesis of sn-2 palmitate are discussed.
基金gratefully acknowledge the Doctoral Scientific Research Start-up Foundation from Henan University of Technology(2021BS015).
文摘Human milk fat substitute is an important structured lipid intended for infant formula. This present study compared human milk fat substitute from R. opacus with commercial product and evaluated whether it is qualified for infant formula. It was proven that both human milk fat substitute and commercial product were mainly composed of C16:0 and C18:1 in total fatty acid composition. Human milk fat substitute contained high level of C16:0 at sn -2 position (54.57%) and high level of 1,3-dioleoyl-2-palmitoylglycerol (37.94%), which was in accordance with human milk fat and commercial product. In addition, relative high level of odd chain fatty acids (1.56%) and C16:1 (7.27%) was identified, which made human milk fat substitute unique. Human milk fat substitute was short of medium-chain fatty acids, such as C8:0, C10:0 and polyunsaturated fatty acids, such as, eicosapentaenoic acid and docosahexaenoic acid. In conclusion, human milk fat substitute from R. opacus was qualified as a component for infant formula in term of fatty acid composition and triacylglycerol.
