To regulate human organ transplant, guarantee the quality and safety of the operation, protect the health of the people, the Health Ministry of China issued Regulations governing Clinical Application of Human Organ Tr...To regulate human organ transplant, guarantee the quality and safety of the operation, protect the health of the people, the Health Ministry of China issued Regulations governing Clinical Application of Human Organ Transplant on March 27, 2006, which is to be put into effect on the coming July 1. The regulations contain 5 chapters and 47 articles including general principles, registration of diagnosis and treatment, clinical management, supervision, and supplementary items.展开更多
BACKGROUND: The highly specific vascular endothelialgrowth factor (VEGF) induces the growth of vascular en-dothelial cell. This study was to construct the eukaryoticexpression plasmid of vascular endothelial growth fa...BACKGROUND: The highly specific vascular endothelialgrowth factor (VEGF) induces the growth of vascular en-dothelial cell. This study was to construct the eukaryoticexpression plasmid of vascular endothelial growth factorl65(VEGF165) and observe its expression in vascular smoothmuscles (VSMCs).METHODS: The primers were designed and synthesizedaccording to the gene sequences of human VEGF165. TheVEGF165 gene was obtained from umbilic artery tissue bythe method of RT-PCR, then it was cloned to eukaryoticexpression plasmid pBudCE4.1 by recombination strategy.The eukaryotic expression plasmid named pBudCE4.1/VEGF165 was identified by restriction enzyme digestion,and was sequenced. The pBudCE4.1/VEGF165 was trans-fected into VSMCs by using lipofection. The VEGF165 ex-pression of mRNA and protein was detected by RT-PCRand Western blot respectively.RESULTS: VEGF165 was shown about 576bp by RT-PCR.Sequencing revealed the amplified VEGF165 gene was iden-tical with that in the GeneBank. Restrictive enzyme (HindBam HI) digestion analysis showed that recombinantexpression plasmid pBudCE4. l/tVEGF165 had been con-structed successfully. The expression of VEGF165 at mRNAand protein levels in the transformed VSMCs had beendemonstrated by RT-PCR and Western blot.CONCLUSIONS: The recombinant eukaryotic expressionplasmid pBudCE4.1/VEGF165 has been successfully con-structed and expressed in transformed VSMCs. The presentstudy has laid a foundation for VEGF165 gene therapy ofvascular stenosis in the transplant organ.展开更多
戊型肝炎病毒(hepatitis E virus,HEV)引起的戊型肝炎一直被认为是一种急性自限性感染过程,然而最近发现,器官移植、人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者等免疫力低下的患者感染HEV能够发展为慢性戊型肝炎.有些...戊型肝炎病毒(hepatitis E virus,HEV)引起的戊型肝炎一直被认为是一种急性自限性感染过程,然而最近发现,器官移植、人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者等免疫力低下的患者感染HEV能够发展为慢性戊型肝炎.有些慢性戊型肝炎病例肝脏快速纤维化而出现肝硬化,并最终导致肝脏衰竭.患者免疫抑制作用减弱,免疫功能恢复有助于体内持续存在的HEV清除.研究发现干扰素和利巴韦林对治疗慢性戊型肝炎有效.展开更多
目的探讨在当前心死亡器官捐献(donation after cardiac death,DCD)来源的器官移植方案下手术室人力资源调配的新模式及其临床实践的效果。方法回顾性分析2014年10月至2016年3月在我院施行DCD来源的肝、肾移植手术的100例患者临床资料,...目的探讨在当前心死亡器官捐献(donation after cardiac death,DCD)来源的器官移植方案下手术室人力资源调配的新模式及其临床实践的效果。方法回顾性分析2014年10月至2016年3月在我院施行DCD来源的肝、肾移植手术的100例患者临床资料,分为对照组(由移植专业组成员作为移植手术承担者的模式)和观察组(结合常规值班基础上的移植备班的模式),比较两组在调配移植手术人员所用时间、器官获取的准备与等待时间、器官到位后开台准备时间、手术时间、手术配合质量及医护人员相关满意度等方面的情况差异。结果实施移植备班模式后,观察组调配移植手术人员所用时间,器官获取的准备与等待时间、器官到位后开台准备时间以及手术时间均较对照组明显缩短(P<0.05),手术室护士劳动强度较对照组明显下降(P<0.05),两组移植医生对术前准备时间、术前物品准备齐全程度以及对护士术中配合质量的满意度评分比较差异无统计学意义(P>0.05)。结论在当前DCD模式下建立完善的移植手术备班管理模式,能够在明显缩短人员调配及手术配合人员术前准备所耗时间的同时,保证了手术配合质量及医生的满意度,降低了护理人员的工作强度。展开更多
文摘To regulate human organ transplant, guarantee the quality and safety of the operation, protect the health of the people, the Health Ministry of China issued Regulations governing Clinical Application of Human Organ Transplant on March 27, 2006, which is to be put into effect on the coming July 1. The regulations contain 5 chapters and 47 articles including general principles, registration of diagnosis and treatment, clinical management, supervision, and supplementary items.
基金This study was supported by grants from the 973 National Basic ResearchProgram of China ( 2003CB515501 ) and the National Natural ScienceFoundation of China (No. 30270514).
文摘BACKGROUND: The highly specific vascular endothelialgrowth factor (VEGF) induces the growth of vascular en-dothelial cell. This study was to construct the eukaryoticexpression plasmid of vascular endothelial growth factorl65(VEGF165) and observe its expression in vascular smoothmuscles (VSMCs).METHODS: The primers were designed and synthesizedaccording to the gene sequences of human VEGF165. TheVEGF165 gene was obtained from umbilic artery tissue bythe method of RT-PCR, then it was cloned to eukaryoticexpression plasmid pBudCE4.1 by recombination strategy.The eukaryotic expression plasmid named pBudCE4.1/VEGF165 was identified by restriction enzyme digestion,and was sequenced. The pBudCE4.1/VEGF165 was trans-fected into VSMCs by using lipofection. The VEGF165 ex-pression of mRNA and protein was detected by RT-PCRand Western blot respectively.RESULTS: VEGF165 was shown about 576bp by RT-PCR.Sequencing revealed the amplified VEGF165 gene was iden-tical with that in the GeneBank. Restrictive enzyme (HindBam HI) digestion analysis showed that recombinantexpression plasmid pBudCE4. l/tVEGF165 had been con-structed successfully. The expression of VEGF165 at mRNAand protein levels in the transformed VSMCs had beendemonstrated by RT-PCR and Western blot.CONCLUSIONS: The recombinant eukaryotic expressionplasmid pBudCE4.1/VEGF165 has been successfully con-structed and expressed in transformed VSMCs. The presentstudy has laid a foundation for VEGF165 gene therapy ofvascular stenosis in the transplant organ.
文摘戊型肝炎病毒(hepatitis E virus,HEV)引起的戊型肝炎一直被认为是一种急性自限性感染过程,然而最近发现,器官移植、人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者等免疫力低下的患者感染HEV能够发展为慢性戊型肝炎.有些慢性戊型肝炎病例肝脏快速纤维化而出现肝硬化,并最终导致肝脏衰竭.患者免疫抑制作用减弱,免疫功能恢复有助于体内持续存在的HEV清除.研究发现干扰素和利巴韦林对治疗慢性戊型肝炎有效.
文摘目的探讨在当前心死亡器官捐献(donation after cardiac death,DCD)来源的器官移植方案下手术室人力资源调配的新模式及其临床实践的效果。方法回顾性分析2014年10月至2016年3月在我院施行DCD来源的肝、肾移植手术的100例患者临床资料,分为对照组(由移植专业组成员作为移植手术承担者的模式)和观察组(结合常规值班基础上的移植备班的模式),比较两组在调配移植手术人员所用时间、器官获取的准备与等待时间、器官到位后开台准备时间、手术时间、手术配合质量及医护人员相关满意度等方面的情况差异。结果实施移植备班模式后,观察组调配移植手术人员所用时间,器官获取的准备与等待时间、器官到位后开台准备时间以及手术时间均较对照组明显缩短(P<0.05),手术室护士劳动强度较对照组明显下降(P<0.05),两组移植医生对术前准备时间、术前物品准备齐全程度以及对护士术中配合质量的满意度评分比较差异无统计学意义(P>0.05)。结论在当前DCD模式下建立完善的移植手术备班管理模式,能够在明显缩短人员调配及手术配合人员术前准备所耗时间的同时,保证了手术配合质量及医生的满意度,降低了护理人员的工作强度。
