Sintered strontium hydroxyapatite (Sr-HAP) and hydroxapatite (HAP) werekinds or apatite ceramics as substitutes of bone tissues.They should be safety to human body.Sr-HAP and HAP were co-cultured with human osteoblast...Sintered strontium hydroxyapatite (Sr-HAP) and hydroxapatite (HAP) werekinds or apatite ceramics as substitutes of bone tissues.They should be safety to human body.Sr-HAP and HAP were co-cultured with human osteoblast like cells for 2, 4,and 6 days invitro. Effect of Sr-HAP and HAP on cell growth numbers with thymidine incorporationtest. In order to estimated cytotoxicities of materials,relative cell growth rate (RGR) werecalculated from data and score of cytotoxicties were assaied. Results showed cell growth numbers and rate of 3H-thymidine incorporation in Sr-HAP and HAP groups were the same asthat of blank control group. Values were increased with the increase of co-cultured time.There were no inhibition of cell growth and DNA syntheses in human osteoblast like cells. Accoding to RGR and score of cytotoxicity degree,all values were qurlified with sdandard ofbiomaterials. Results suggested these qurlified with sdandard or biomaterials. Results suggested these apatite ceramics had no obvious cytotoxicities.展开更多
文摘Sintered strontium hydroxyapatite (Sr-HAP) and hydroxapatite (HAP) werekinds or apatite ceramics as substitutes of bone tissues.They should be safety to human body.Sr-HAP and HAP were co-cultured with human osteoblast like cells for 2, 4,and 6 days invitro. Effect of Sr-HAP and HAP on cell growth numbers with thymidine incorporationtest. In order to estimated cytotoxicities of materials,relative cell growth rate (RGR) werecalculated from data and score of cytotoxicties were assaied. Results showed cell growth numbers and rate of 3H-thymidine incorporation in Sr-HAP and HAP groups were the same asthat of blank control group. Values were increased with the increase of co-cultured time.There were no inhibition of cell growth and DNA syntheses in human osteoblast like cells. Accoding to RGR and score of cytotoxicity degree,all values were qurlified with sdandard ofbiomaterials. Results suggested these qurlified with sdandard or biomaterials. Results suggested these apatite ceramics had no obvious cytotoxicities.
