Detection of hepatitis B viral DNA in liver with polymerase chain reaction

The sensitivity of PCR was determined for detection of HBV DNA in paraffin-embed-ded liver tissue with different methods for sample preparation.Of 8 cases of HBeAg-positiveHBV infection,HBV DNA was detected in 6 by extracting DNA from both frozen and dewaxedsamples,but in none by direct reaction.Of 12 cases subjected to Southern blot hybridization,HBV DNA was detected in 7 by this technique,in 10 by PCR with both methods of DNA extrac-tion and in 3 by direct PCR.The results showed that PCR was sensitive and was comparablewith blot hybridization in detecting intrahepatic HBV DNA.In comparison between differentmethods of sample preparation,the viral detection rate from the dewaxed samples was near thatfrom the frozen ones,while by the direct reaction HBV DNA could be detected only in a fewsamples with high level of infection.

THE DETECTION OF P53 GENE IN HUMAN CERVICAL CARCINOMA WITH AND WITHOUT HUMAN PAPILLOMAVIRUS INFECTION

The special primers Of p53 exon 7 as wed as HPV16 E6 and E7 ORFs (Opening Rending Frame) were used with PCR, PCR-SSCP technique, and 35 specimens of cervical carcinoma were examined. The results were as follows: ① HPV16 E6, E7 DNA was found in 25/35 specimens (71. 4%),which proved again HPV16 Infection an important event in cervical carcinogenesis. However only 11/35 (31.42% ) bad E6 and E7 ORFs simultaneously, 3/35 (8. 57%) and 11/35 (31. 42% ) had only E6 or E7 respectively. ② No mutation and LOH (Loss of Heterozygote) of p53 exon 7 were found in allof 35 specimens. Additionally in the present study, we developed a non-isotopic PCR-SSCP method.

Human papillomavirus DNA and P16～(INK4A) expression in concurrent esophageal and gastric cardia cancers

AIM:To investigate the relationship between human papillomavirus (HPV) infection and concurrent esophagus and gastric cardia cancer from the same patient (CC) and examine the significance of P16 INK4A protein expression.METHODS:Polymerase chain reaction was used to detect the presence of HPV type16 (HPV16).The expression of P16 INK4A protein was detected using immunohistochemistry.RESULTS:Among the CC specimens,HPV16-DNA was found in eight cases of esophageal squamous cell carcinoma (ESCC) and five cases of gastric cardia adenocarcinoma (GCA),respectively (47% vs 29%),and two of both ESCC and GCA.P16 INK4A was highly expressed in both ESCC and GCA.In the HPV-associated positive CC,higher P16 INK4A expression was observed in the GCA than in the ESCC (75% vs 25%,P < 0.05).CONCLUSION:HPV16 as a correlated risk factor may play an important role in the development of ESCC and GCA.P16 INK4A may be a screening index in the HPVassociated carcinoma of gastric cardia.

Comparison of Human Papillomavirus Detection and Genotyping with Four Different Prime Sets by PCR-Sequencing

Objective To assess and compare the Human Papillomavirus（HPV） detection efficiency and the potential clinical utility of PCR sequencing‐based technology.Methods Four HPV consensus primer sets（GP5＋/6＋,MGP,MY09/11,and PGMY09/11） were used in order to amplify a broad spectrum of HPV types for HPV infection in 325 cervical samples and the PCR products were sequenced afterwards for the HPV genotyping.Results The HPV‐positive rate was 75.4%,of which 35.5% harbored more than one HPV genotype.A total of 36 different genotypes was found,with HPV 16（24.1%） being the most prevalent,followed by HPV 58（13.3%） and HPV 52（9.6%）.There were substantial to almost perfect agreements between different primer sets regarding HPV detection efficiency,with the kappa value varying from 0.751 to 0.925,MGP,and PGMY09/11 were the most effective in detecting multiple infections（P0.001）.With each of the primer sets,a board range of HPV types could be identified,though there were several differences for a few genotypes.Conclusion The substantial agreement between PCR‐sequencing and HC2 for the detection of high‐risk HPV（kappa=0.761） indicated that PCR‐sequencing is also suitable for routine HPV screening.

Urine versus brushed samples in human papillomavirus screening： study in both genders

Aim： To investigate whether urine is a good medium for screening and whether there is a correlation between the amount of extracted DNA and human papillomavirus （HPV）-positivity. Methods： In the present study, 30 first-voided urine （FVU） specimens and 20 urethroglandular swabs using cervex-brushes from male partners of HPV-positive patients, and 31 FVU specimens and 100 liquid-based cervix cytology leftovers sampled with cervix-brushes from HPV-positive women were examined for the presence of β-globin. Oncogenic HPV were detected using type-specific PCR. Results： β-globin was found in all the brushed samples, whereas it was found in only 68.9% of the FVU specimens. HPV-PCR was positive in 60.0% of the male brushes, in 29% of the female brushes and in 0% of the male FVU specimens. DNA concentration was, respectively, 0.9998 ng/μL, 37.0598 ng/μL and 0.0207 ng/μL. Conclusion： Urine is not a good tool for HPV detection, probably because the low DNA concentration reflects a low amount of collected cells. β-globin is measurable in FVU by real time quantitative PCR, but the DNA concentration is lower compared to brush sampling for both genders. β-globin-positivity of urethral and cervical swabs is 100%, showing a higher mean concentration of DNA, leading to a higher detection rate of HPV. This is the first article linking DNA- concentration to the presence of HPV.

Asymptomatic Genital Infection of Human Papillomavirus in Pregnant Women and the Vertical Transmission Route

To further investigate the vertical transmission route of human papillomavirus (HPV) and the indication for the choice of mode of delivery, the infective status of 152 asymptomatic pregnant wemen and the maternal-fetal transmission were studied. By using general primers in polymerase chain reaction (GP-PCR) combined with restriction fragment length polymorphism analysis, HPV DNA positive rate in cervical secretions and venous blood in asymptomatic pregnant women was 36.21 % and 52.78 %, respectively, and the identified genotypes were mainly HPV_16 and _18. The maternal-fetal transmission rate of HPV via genital tract as well as blood was 40.91 % and 57.89 %, respectively. It was concluded that besides the transmission route of genital tract and amniotic fluid, there was also transplacental transmission of HPV in utero. Therefore,in our opinion, it is not an absolut indication to perform a cesarean delivery for the pregnant women with HPV asymtomatic genital infection.

Comparison of the Tellgenplex HPV DNA test with the PCR-reverse dot blot assay for human papillomavirus genotyping

Objective: To access the performance of the Tellgenplex human papillomavirus(HPV) DNA test compared to the polymerase chain reaction-reverse dot blot(PCR-RDB) assay for the HPV genotyping.Methods: Sixty cervical swab samples were genotyped by the Tellgenplex HPV DNA test and the PCR-RDB assay.The Tellgenplex HPV DNA test and the PCR-RDB assay can detect 26 and 23 HPV genotypes, respectively.Each sample showed discrepancy was genotyped using sequencing.Results: The percent agreement between the two tests ranged from 83.3% to 100.0% according to different genotype.This showed perfect agreement(>0.81) for high-risk HPV genotypes(35, 39, 45, 53, 56, 59, 66, 68, and 82), substantial agreement(>0.65) for high-risk HPV genotypes(16, 18, 33, 52, and 58) and low-risk HPV genotype 43 between the two assays by the kappa analysis.The positive rates of the two assays for frequent HPV genotypes(16, 35, 39, 45, 52, 53, 58, 59, 66, and 82) were not statistically different, but the PCR-RDB assay showed higher positive rates than the Tellgenplex HPV DNA test for HPV genotypes 81(P<0.05).As for more than 10 positive results by the Tellgenplex HPV DNA test and/or the PCR-RDB assay, the PCR-RDB assay showed higher relative sensitivity and specificity than the Tellgenplex HPV DNA test for the three HPV genotypes(16, 52, and 81).All HPV genotypes that can be detected by only the Tellgenplex HPV DNA test(HPV genotypes 44 and 55) were confirmed by sequencing.Conclusions: In conclusion, our results demonstrated that the PCR-RDB assay which can detect more multiple HPV genotypes in each specimen shows higher relative sensitivity and specificity than the Tellgenplex HPV DNA test, which makes it a better option for routine clinical use.

Evaluation of Human Papillomavirus Infection among Women Using Pap Smear and PCR in Shiraz, Iran

Human papillomaviruses （HPVs） infect mucosal and cutaneous epithelia and cause malignancy and neoplastic lesions. These viruses cause 25,000 deaths per year from cervical cancer most often in developing countries. This major public health problem makes them important targets in the researches of papillomavirus detection methods. Since the early diagnosis of this virus infection would prevent neoplasia and cervical cancer, therefore in this study the combination of molecular and cytological methods were used to show the occurrence of the infection in women referred to Baghiatollah clinic of Shiraz. The results showed out of 110 cases, two samples were positive by PCR using GP5/6 primers but Pap smears showed only one sample of abnormal cytology. The rest 108 samples were negative by PCR and had normal cytology. The samples （1.82%） in evaluated women. present study showed a low occurrence of HPV infection in cervical

DETECTION OF HUMAN PAPILLOMAVIRUS TYPES 16, 18 DNA RELATED SEQUENCES IN BRONCHOGENIC CARCINOMA BY POLYMERASE CHAIN REACTION

In studying the relationship between human papillomavirus (HPV) and bronchogenic carcinoma, 'high-risk' HPV 16, 18 DNA sequences were detected in samples from 50 lung cancer patients, 18 patients with benign pulmonary diseases and 4 fetal lung tissues by polymerase chain reaction (PCR) and dot-blot hybridization with biotin-labelled probes. The results showed that HPV 16, 18 DNA related sequences were found in 32% of lung cancer specimens, with 10 cases of HPV 16, 5 cases of HPV 18 and 1 case of both types. 48.15% (13 / 27) of squamous cell carcinomas were shown to be positive for HPV 16, 18 DNA. In addition, two adenocarcinomas and one small cell carcinoma were positive for HPV 16 DNA. No specimens from benign diseases tissues and fetal lung tissues showed positive results. These results suggest that primary bronchogenic carcinoma is related to HPV infection.

北京朝阳区28923例女性人乳头瘤病毒感染基因型分析

目的分析北京朝阳区28923例人乳头瘤病毒(HPV)检测结果的感染情况、基因分型及在各年龄段的分布差异,为本地区的宫颈癌(CC)防治及疫苗推广提供参考依据。方法回顾性分析2019年1月至2021年12月在首都医科大学附属北京妇产医院/北京妇幼保健院妇科肿瘤科、计划生育科、妇保妇检科等科室首次就诊且自愿进行多重荧光PCR技术23种HPV基因分型检测的28923例女性患者的人乳头瘤病毒筛查检测结果。结果28923例患者中,检测出HPV感染者11076例,感染率为38.29%;所有HPV感染者中单一型别感染占比24.45%、两重感染占比8.56%、双重以上型别感染占比5.27%;患者年龄从低到高感染率趋势呈U型分布;单一型别感染中高危型HPV(HR-HPV)中最常见的基因型是HPV16型,其次是52、58、53型,单一型别感染中低危型HPV(LR-HPV)中最常见的基因型是HPV81型,其次是42、44、43型;28923例感染者中31~40岁年龄组患者人数最多为8958例(占比31%);检出率小于20岁年龄组最高为55.56%,其次为21~30岁年龄组为42.62%,接下来是大于60岁年龄组为41.88%。结论北京朝阳区总体HR-HPV中感染率最高型别依次是HPV16、52及58型,LR-HPV中感染率最高的型别依次是HPV42、81及44型;年龄上31~50岁组感染率更高,单一HPV感染率高于多重感染,应提高本地区HPV16、52、58型的筛查能力,引起足够重视,有针对性的推广CC疫苗的接种,以便有效降低本地区CC的发生。

HPV诊断中应用PCR检验的效果

目的分析高危型人乳头瘤病毒(HPV)诊断中应用实时聚合酶链式反应(PCR)检验的效果。方法以该院2023年1-12月内收治的101例高危型HPV患者为本次研究对象,所有研究对象先使用第二代杂交式捕获法(HC-Ⅱ)检验,再行实时PCR检验,以病理实验检测结果为金标准,统计对比两种检验方式的诊断效能。结果组间对比,HC-Ⅱ与实时PCR对高危型HPV的诊断特异度差异无统计学意义(P>0.05),但实时PCR对高危型HPV的诊断准确率、灵敏度均显著高于HC-Ⅱ,差异有统计学意义(P<0.05)。结论实时PCR对于高危型HPV感染患者具有较高检出率,可为此类患者的早期治疗提供科学依据,倡导临床应用。

实时PCR检验在高危型人乳头瘤病毒诊断中的应用价值研究

目的:探讨实时聚合酶链式反应(PCR)检验在高危型人乳头瘤病毒诊断中的应用价值.方法:选取2020年1月—2022年1月贵州省黔西南布依族苗族自治州人民医院收治的疑似感染高危型人乳头瘤病毒患者100例作为研究对象,采用随机数字表法分为对照组和观察组,各50例.对照组采用第二代杂交式捕获法,观察组采用实时PCR检验法.以人乳头瘤病毒核酸检测结果为"金标准",比较两种检测方法的诊断结果、诊断效能、检测费用及用时.结果:观察组诊断敏感度、特异度和准确性高于对照组,差异有统计学意义(P<0.05);观察组检测费用低于对照组,检测用时短于对照组,差异有统计学意义(P<0.001).结论:实时PCR检验在高危型人乳头瘤病毒诊断中的应用价值较高,具有良好的敏感度、特异度和准确性,且费用低廉、用时很短,可为患者进一步临床治疗及预后干预后提供依据.

Value and Feasibility of HPV DNA Test in Cervical Scraping Smears

Summary： To investigate the reliability and feasibility of human papillomavirus （HPV） DNA test in cervical scraping smears with polymerase chain reaction （PCR）, 131 cases of cervical scraping specimens were collected, and the positive rates and accuracy of HPV infection were determined in normal subjects and cervical cancer patients. GP5^＋/GP6^＋ and E7 primer pairs designed for detecting HPV L1 and HPV type 16 E7 were tested in this study. Our results showed that positive rates of HPV DNA in normal population and cervical cancer patients were 32.99% and 73.53% respectively and there was significant difference between them （P〈0. 001）. In normal subjects, detection rates of HPV DNA with GP5^＋/GP6^＋ and E7 primer pairs were 27.84 % and 16.49 % respectively, with statistically significant difference between them （P〉0.05）. However the detection rates in cervical cancer patients were 38.24 % and 67.65 % for the two markers, with a significant difference found between them （P〈0.05）. It is concluded that HPV DNA test with PCR for cervical scraping smears was feasible. GP5^＋/GP6^＋ primer pairs may be a useful probe to screen HPV infection in normal population, but they are not sensitive enough in cervical cancer patients. It is suggested that high risk type HPV DNA test was very useful in population with high risk of cervical cancer.

Head-to-head comparison of 7 high-sensitive human papillomavirus nucleic acid detection technologies with the SPF10 LiPA-25 system

Background:The SPF10 LiPA-25 system for human papillomavirus(HPV)detection with high analytical perfor-mance is widely used in HPV vaccine clinical trials.To develop and evaluate more valent HPV vaccines,other comparable methods with simpler operations are needed.Methods:The performance of the LiPA-25 against that of other 7 assays,including 4 systems based on reverse hybridization(Bohui-24,Yaneng-23,Tellgen-27,and Hybribio-16)and 3 real-time polymerase chain reaction(PCR)assays(Hybribio-23,Bioperfectus-21,and Sansure-26),was evaluated in selected 1726 cervical swab and 56 biopsy samples.A total of 15 HPV genotypes(HPV 6,11,16,18,31,33,35,39,45,51,52,56,58,59,and 66)were considered for comparison for each HPV type.Results:Among the swab samples,compared to LiPA-25,compatible genotypes were observed in 94.1%of samples for Hybribio-23,92.8%for Yaneng-23,92.6%for Bioperfectus-21,92.4%for Hybribio-16,91.3%for Sansure-26,89.7%for Bohui-24,and 88.0%for Tellgen-27.The highest overall agreement of the 15 HPV genotypes combined was noted for Hybribio-23(κ=0.879,McNemar’s test:P=0.136),followed closely by Hybribio-16(κ=0.877,P<0.001),Yaneng-23(κ=0.871,P<0.001),Bioperfectus-21(κ=0.848,P<0.001),Bohui-24(κ=0.847,P<0.001),Tellgen-27(κ=0.831,P<0.001),and Sansure-26(κ=0.826,P<0.001).Additionally,these systems were also highly consistent with LiPA-25 for biopsy specimens(all,κ>0.897).Conclusions:The levels of agreement for the detection of 15 HPV types between other 7 assays and LiPA-25 were all good,and Hybribio-23 was most comparable to LiPA-25.The testing operation of HPV genotyping should also be considered for vaccine and epidemiological studies.

3053例就诊女性人乳头瘤病毒感染现状分析

目的 通过回顾并调查分析宜昌地区不同年龄段就诊女性人乳头瘤病毒(HPV)的感染和流行情况,为防治HPV提供理论依据。方法 选择国药葛洲坝中心医院2021年1月—2022年6月收治的3 053例就诊女性作为研究对象,根据年龄分为6组,分别为21~30岁组(229例)、31~40岁组(568例)、41~50岁组(1 102例)、51~60岁组(871例)、61~70岁组(235例)、>70岁组(48例)。采用聚合酶链反应(PCR)-反向杂交法进行HPV基因分型检测,统计并分析不同年龄组女性的HPV感染情况。结果 3 053例受检者中共检出HPV阳性421例,阳性率为13.79%。其中单一亚型感染328例,占所有感染的77.91%(328/421);双重亚型感染70例,占所有感染的16.63%(70/421);多重亚型感染23例,占所有感染的5.46%(23/421)。对不同年龄组女性的HPV感染阳性率进行统计分析,结果显示差异均有统计学意义,其中21~30岁组和51~60岁组的阳性率分别为18.34%(42/229)、16.19%(141/871),明显高于其他各组,分别为31~40岁组12.32%(70/568)、41~50岁组12.43%(137/1 102)、61~70岁组10.64%(25/235)、>70岁组12.50%(6/48)。高危亚型HPV的阳性检出率为10.12%(309/3 053),其中最常见的感染型别为HPV52亚型,共74例,占比为13.58%(74/545);其次是HPV58亚型,共45例,占比为8.26%(45/545);HPV16亚型共33例,占比为6.06%(33/545)。低危亚型以HPV44亚型居多,共41例,占比为7.52%(41/545);其次为HPV42亚型,共34例,占比为6.24%(34/545)。疑似高危型以HPV53亚型最多,占比为8.26%(45/545)。结论 宜昌地区不同年龄段女性的HPV感染情况有所不同,以高危型感染和单一感染为主,宫颈癌防治策略应针对不同年龄人群开展HPV基因亚型常规检测。

荧光定量PCR与全自动生物芯片法检测人乳头瘤病毒的一致性研究

背景 人乳头瘤病毒(human papillomaviruses,HPV)分型检测是宫颈癌筛查的重要手段之一,但技术人员短缺及实验室硬件设施不足对其应用造成了不利影响。目的 以荧光定量聚合酶链式反应(PCR)为参考,考察全自动生物芯片法在HPV分型检测中的诊断性能。方法 针对常规进行荧光定量PCR检测的临床样本,每一种型别均采用抽签法进行简单随机抽样,选中的样本使用全自动生物芯片法检测,采用Kappa检验比较两种方法的检测结果是否存在差异。针对不一致结果,通过E6/E7区的扩增产物测序进行确认。结果 从4 589例样本中选择124例,其中HPV阴性50例,HPV阳性74例,按照阳性型别计算共99个测试。按照样本阴性、阳性计算,两种方法的结果完全一致,Kappa值为1.000(P<0.000 1)。按照样本型别计算,全自动生物芯片法的敏感度、特异度、阳性预测值、阴性预测值和总体符合率分别为98.0%、92.6%、96.0%、96.2%和96.1%,Kappa值为0.913(P<0.000 1),提示两种方法的结果一致性良好。两种方法不一致的结果集中在6例阳性样本之中,39型和59型各1例,52型和68型各2例,在重复验证及测序分析中均得到了确认。此外,全自动生物芯片法还发现了18例不被荧光定量PCR方法涵盖的型别,能够为临床治疗提供更丰富的信息。结论 全自动生物芯片法与荧光定量PCR法在HPV的分型检测中具有很好的一致性,对于技术人员短缺及不具备标准PCR实验室的医疗机构,全自动生物芯片法具有良好的应用前景。

细辛抗人乳头瘤病毒的作用研究

目的 :筛选细辛抗人乳头瘤病毒 (HPV)的有效部位。方法 :用系统溶媒 (石油醚、乙醚、乙酸乙酯、正丁醇、乙醇、蒸馏水 )对细辛依次进行提取 ,应用荧光定量聚合酶链反应技术 (FQ PCR)对各提取物进行体外抗病毒药效学试验 ,检测离体尖锐湿疣皮损中HPV DNA的扩增情况。结果 :HPV DNA经细辛水提物作用后 ,其PCR扩增检测为阴性 ,最低有效浓度为 0 4 g/ml,而细辛其它提取部位PCR扩增检测为阳性。结论

结直肠肿瘤中人乳头瘤病毒感染的基因分析

目的高危型人乳头瘤病毒(human papillomavirus,HPV)感染是宫颈癌的主要致病原因,现已发现HPV感染可诱发人类的许多恶性肿瘤。文中旨在探讨高危型HPV在结直肠肿瘤(colorectal tumor,CRT)中的感染情况以及与HPV感染的关系。方法从203例结直肠肿瘤石蜡组织标本中提取16、18型HPV的基因,其中结直肠绒毛-管状腺瘤(colorectal villus-tubiform adenoma,CRVTAN)83例、结直肠腺癌(colorectal adenocarcinoma,CRAC)120例,采用聚合酶链反应进行基因扩增检测,并对其患者进行临床病理资料分析。结果 83例CRVTAN总的HPV感染率为57.83%(48/83)、120例CRAC总的HPV感染率为75.00%(90/120),且与HPV感染密切相关(r分别为23.4、47.3,P<0.01)。结论结直肠肿瘤与HPV感染有着密切的关系,无论腺瘤、还是腺癌,直肠及乙状结肠都是结直肠HPV的高感染部位,这合理的解释了长期以来直肠、乙状结肠腺癌在CRAC中的高发病率现象。

FQ-PCR检测宫颈涂片剩余细胞内HPV16、18型感染的临床意义

目的 :探讨实时荧光定量聚合酶链反应 (FQ PCR)检测宫颈涂片剩余细胞内人乳头瘤病毒 (HPV) 16、18型感染 ,建立快速、灵敏的检测方法的临床意义。方法 :采用FQ PCR技术检测 12 4例异常宫颈细胞和组织标本HPV16、18型感染状况。结果 :细胞学诊断低度鳞状上皮内病变 (LSIL) 4 5例 ,其中FQ PCR检测宫颈细胞标本HPV16、18型DNA阳性14例 (阳性率 31.1% ) ;高度鳞状上皮内病变 (HSIL) 38例 ,HPV16、18型DNA阳性 2 6例 (6 8.4 % ) ;未明确诊断意义的不典型鳞状上皮细胞 (ASCUS) 30例 ,HPV16、18型DNA阳性 10例 (33.3% ) ;鳞状细胞癌 9例 ,HPV16、18型DNA阳性 8例 (88.9% )。组织学检查诊断CINⅠ级 6 7例 ,其中FQ PCR检测活检组织标本HPV16、18型DNA阳性 2 8例 (阳性率 4 1.8% ) ;CINⅡ 14例 ,HPV16、18型DNA阳性 9例 (6 4 .3% ) ;CINⅢ级 11例 ,HPV16、18型DNA阳性 8例 (72 .7% ) ;鳞状细胞癌 2 3例 ,HPV16、18型DNA阳性 17例 (73.9% )。细胞和活检组织两种标本检出的HPV16、18型DNA阳性率差异无显著性 (χ2=1.5 4 ,P >0 .0 5 )。结论 :FQ PCR方法检测宫颈细胞标本HPV16、18型感染 ,具有快速、简便、灵敏度高、特异性强等优点 。

HR-HPV DNA PCR联合TCT检测对宫颈癌前病变的诊断价值

目的探讨高危型人乳头瘤病毒(HR-HPV)DNA聚合酶链反应(PCR)联合液基薄层细胞学检查(TCT)对宫颈癌前病变筛查的应用与诊断价值。方法对540例TCT结果为非典型鳞状上皮细胞(ASCUS)及以上的患者进行HR-HPV DNA PCR检测,并对检测结果进行分析。结果 TCT阳性合并HPV阳性患者共259例,其中ASCUS、低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)、鳞状细胞癌(SCC)的例数(阳性率)分别为155例(59.8%)、81例(31.3%)、15例(5.8%)、8例(3.1%);259例患者中ASCUS、LSIL、HSIL、SCC合并HR-HPV的阳性例数(阳性率)分别为28例(18.1%)、20例(24.7%)、10例(66.7%)、8例(100.0%)。HR-HPV和TCT单独检测的敏感性与两者联合检测的敏感性比较,差异具有统计学意义(P<0.05)。HR-HPV、TCT及两者联合检测的阳性预测值分别为25.5%、11.1%、28.2%,阴性预测值分别为68.8%、59.2%、40.8%。结论TCT与HR-HPV DNA PCR检测是筛查宫颈病变的有效方法,能最大程度地发现宫颈异常细胞并及时发现宫颈癌的诱因。