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Analysis of Single Nucleotide Polymorphism in Human Paraoxonase 1 Gene(Q192R) with Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry 被引量:1
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作者 SUN Ya-dong SUN Shu-chen +2 位作者 WANG Zhi YANG Yang ZHANG Jin 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2006年第3期394-396,共3页
Introduction Single nucleotide polymorphisms (SNPs) are the most abundant DNA markers in the human genome occurring at a frequency of one in every 500--1000 nucleotides. A variety of methods have been used for the ... Introduction Single nucleotide polymorphisms (SNPs) are the most abundant DNA markers in the human genome occurring at a frequency of one in every 500--1000 nucleotides. A variety of methods have been used for the analysis of single nucleotide polymorphisms, including restriction fragment length polymorphism (RFLP), direct sequencing by using laser-induced fluorescence detectionTM, fluorescence energy transfer, MALDI-TOF MS combined with primer extension or invasive cleavage, and fluorescence polarization. During the past two decades, mass spectrometry has become a very popular tool in the analysis of biomolecules and is perfectly suited to the analysis of single nucleotide polymorphisms (SNPs) due to its speed, low cost, and accuracy. In this work, we used MALDI TOF mass spectrometry to detect the fragments of restriction endonuclease hydrolysis of PCR products flanking a SNP located at paraoxonase 1(Q192R). Compared with electrophoresis, this method requires less time of analysis and possess a higher accuracy. 展开更多
关键词 Single nucleotide polymorphism human paraoxonase 1 gene Matrix-assisted laser desorption/ionizationtime-of-flight mass spectrometry
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