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The Effect of Connective Tissue Growth Factor on Human Renal Tubular Epithelial Cell Transdifferentiation 被引量:2
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作者 张春 朱忠华 邓安国 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2004年第4期350-353,共4页
To investigate the role of connective tissue growth factor (CTGF) in transdifferentiation of human renal tubular epithelial cell (HKC), in vitro cultured HKC cells were divided into 3 groups: negtive control, low dose... To investigate the role of connective tissue growth factor (CTGF) in transdifferentiation of human renal tubular epithelial cell (HKC), in vitro cultured HKC cells were divided into 3 groups: negtive control, low dose CTGF-treated group (rh CTGF, 2.5 ng/ml) and high dose CTGF-treated (rhCTGF, 5.0 ng/ml). Then the expression of α-smooth muscle actin (α-SMA) were assessed by indirect immuno-fluorescence, and the percentage of α-SMA positive cells were assessed by flow cytometry. RT-PCR were also performed to examine the mRNA level of α-SMA. Upon the stimulation of different concentrations of rhCTGF, the expression of α-SMA were markedly stronger than that in negative controls. The percentages of α-SMA positive cells were significantly higher in the stimulated groups than that of negative controls (38.9 %, 65.5 % vs 2.4 %, P<0.01) .α-SMA mRNA levels were also up-regulated by the stimulation of rhCTGF (P<0.01). These results suggest that CTGF can promote the transdifferentiation of human renal tubular epithelial cells towards myofibroblast (Myo-F). 展开更多
关键词 connective tissue growth factor human renal tubular epithelial cell TRANSDIFFERENTIATION
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7,8-dihydroxyflavone protects human renal proximal tubular cells from hypoxia injury via inhibiting endoplasmic reticulum stress
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作者 马蕊 《China Medical Abstracts(Internal Medicine)》 2017年第1期49-,共1页
Objective To observe the effects of 7,8-dihydroxyflavone(7,8-DHF)on hypoxia induced endoplasmic reticulum stress(ERS)in human proximal tubular epithelial cells(HK-2).Methods The mRNA level of ERS associated biomarkers... Objective To observe the effects of 7,8-dihydroxyflavone(7,8-DHF)on hypoxia induced endoplasmic reticulum stress(ERS)in human proximal tubular epithelial cells(HK-2).Methods The mRNA level of ERS associated biomarkers was evaluated by RT-PCR assay 展开更多
关键词 DHF ERS HK dihydroxyflavone protects human renal proximal tubular cells from hypoxia injury via inhibiting endoplasmic reticulum stress Akt
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