In William Faulkner's A Rose for Emily,we can read his attitude to Southern traditional civilization and patriarchal social system.William Faulkner revealed that the root of women's tragic fate was the brutali...In William Faulkner's A Rose for Emily,we can read his attitude to Southern traditional civilization and patriarchal social system.William Faulkner revealed that the root of women's tragic fate was the brutality and cruelty of Southern tradition,and opened out corrupting and perverting of Southern aristocrat according drawing many vivid characters.This thesis analyzes these insensible figures to fulminate against the inhuman society tradition in South America at that time.展开更多
[Objectives] To investigate the pharmacologic effects of active components from A. membranaceus on human esophageal cancer HCE-4 cells and its apoptosis mechanism. [Methods] The viabilities of HCE-4 cells were measure...[Objectives] To investigate the pharmacologic effects of active components from A. membranaceus on human esophageal cancer HCE-4 cells and its apoptosis mechanism. [Methods] The viabilities of HCE-4 cells were measured by MTT assay. The induction of active components from A. membranaceus on apoptosis of HCE-4 cells was detected by Annexin V-FITC/PI double staining. The apoptotic-related protein expression levels were determined by Western blotting. [Results] Formononetin and astragaloside IV suppressed the proliferation of HCE-4 cells in a dose-dependent manner. The Annexin V-FITC/PI double staining results showed that formononetin and astragaloside IV could induce HCE-4 cells apoptosis in a time-dependent manner. The Western blotting results showed that formononetin and astragaloside IV could significantly down-regulate p-AKT,pro-caspase-3,and increase cle-caspase-3 protein expression in HCE-4 cells. [Conclusions]Active components from A. membranaceus such as formononetin and astragaloside IV significantly inhibited the proliferation of human esophageal cancer HCE-4 cells by inducing mitochondrial dependent apoptosis via AKT signaling pathway.展开更多
文摘In William Faulkner's A Rose for Emily,we can read his attitude to Southern traditional civilization and patriarchal social system.William Faulkner revealed that the root of women's tragic fate was the brutality and cruelty of Southern tradition,and opened out corrupting and perverting of Southern aristocrat according drawing many vivid characters.This thesis analyzes these insensible figures to fulminate against the inhuman society tradition in South America at that time.
基金Supported by the Nature Science Foundation of Heilongjiang Province of China(LC2015036)the Program of Cultivation and Support Projects of Heilongjiang Bayi Agricultural University(XA2015-04)+2 种基金the Research Project of Heilongjiang Bayi Agricultural University(XYB2013-24)the Postdoctoral Scientific Research Foundation of Heilongjiang Province of China(LBH-Q13132)the Scientific Research Innovation Program for College Graduates of Heilongjiang Bayi Agricultural University(YJSCX2017-Y72)
文摘[Objectives] To investigate the pharmacologic effects of active components from A. membranaceus on human esophageal cancer HCE-4 cells and its apoptosis mechanism. [Methods] The viabilities of HCE-4 cells were measured by MTT assay. The induction of active components from A. membranaceus on apoptosis of HCE-4 cells was detected by Annexin V-FITC/PI double staining. The apoptotic-related protein expression levels were determined by Western blotting. [Results] Formononetin and astragaloside IV suppressed the proliferation of HCE-4 cells in a dose-dependent manner. The Annexin V-FITC/PI double staining results showed that formononetin and astragaloside IV could induce HCE-4 cells apoptosis in a time-dependent manner. The Western blotting results showed that formononetin and astragaloside IV could significantly down-regulate p-AKT,pro-caspase-3,and increase cle-caspase-3 protein expression in HCE-4 cells. [Conclusions]Active components from A. membranaceus such as formononetin and astragaloside IV significantly inhibited the proliferation of human esophageal cancer HCE-4 cells by inducing mitochondrial dependent apoptosis via AKT signaling pathway.
