BACKGROUND Gestational diabetes mellitus(GDM)is a concern due to its rapid increase in incidence in recent years.AIM To investigate the correlation and predictive value of serum pregnancyassociated plasma protein A(PA...BACKGROUND Gestational diabetes mellitus(GDM)is a concern due to its rapid increase in incidence in recent years.AIM To investigate the correlation and predictive value of serum pregnancyassociated plasma protein A(PAPP-A),triglyceride(TG),and 25-hydroxyvitamin D[25-(OH)D]with GDM in early pregnancy.METHODS A total of 99 patients in early pregnancy admitted to Peking University International Hospital from November 2015 to September 2017 were included,and underwent a fasting glucose test and oral glucose tolerance test screening at 24-28 wk of pregnancy.Of these cases with GDM,51 were assigned to group A and the remaining 48 cases without GDM were enrolled in group B.Serum PAPP-A,TG and 25-(OH)D in the two groups were compared and their correlation with blood sugar was analyzed.In addition,their diagnostic value in GDM was determined using receiver operating characteristic(ROC)curve analysis.RESULTS Group A had markedly lower serum PAPP-A and 25-(OH)D levels and a significantly higher serum TG level than group B,with statistical significance(P<0.05).Furthermore,Pearson analysis identified that PAPP-A and 25-(OH)D levels were negatively correlated with fasting blood glucose(FBG)levels(r=-0.605,P<0.001),(r=-0.597,P<0.001),while TG and FBG levels were positively correlated(r=0.628,P<0.001).The sensitivity,specificity,area under the curve(AUC)and optimal cut-off value of serum PAPP-A level in the diagnosis of GDM were 72.55%,82.35%,0.861 and 16.340,respectively,while the sensitivity of TG in diagnosing GDM was 86.27%,the specificity was 66.67%,the AUC was 0.813,with an optimal cut-off value of 1.796.The corresponding sensitivity,specificity,AUC and optimal cut-off value of serum 25-(OH)D were 64.71%,70.59%,0.721 and 23.140,respectively.Moreover,multivariate logistic regression analysis revealed that FBG,vascular endothelial growth factor,Flt-1,serum PAPP-A,TG,and 25-(OH)D were related risk factors leading to GDM in patients.CONCLUSION Serum PAPP-A,TG,and 25-(OH)D levels are all correlated with blood glucose changes in GDM,and are independent factors affecting the occurrence of GDM and have certain value in the diagnosis of GDM.展开更多
We enrolled 23 Japanese men (age: 76.0 ± 8.7) and 17 women (age: 78.3 ± 9.3) in this study. The physical function of even a person getting on a wheelchair could be tested in all subjects. Blood was collected...We enrolled 23 Japanese men (age: 76.0 ± 8.7) and 17 women (age: 78.3 ± 9.3) in this study. The physical function of even a person getting on a wheelchair could be tested in all subjects. Blood was collected by venipuncture and the serum 1, 25-hydroxy vitamin D (1, 25OHD) concentration was measured. The Mini-Mental State Examination (MMSE) and the Montreal Cognitive Assessment-Japanese version (MoCA-J) was used for the cognitive function test. Physical function was measured objectively using the Timed UP and Go (TUG) and 4-m walking test (4MWS). A significant positive correlation was found between serum 1, 25OHD and MMSE or MoCA-J. It is expected that an elderly person can maintain a mean serum 1, 25OHD level of about 100 pg/mL for preventing early cognitive disorder. In the present study, a significant positive correlation was found between urinary 25-hydroxy vitamin D (25OHD)/creatinine and MMSE or MoCA-J. Our results showed that urinary 25OHD might be a useful biomarker for predicting cognitive disorder. There was a significant negative correlation between serum 1, 25OHD and TUG or 4MWS. These findings suggest that serum 1, 25OHD levels might serve as a useful index to improve cognitive and physical functional impairment.展开更多
Interaction of procainamide hydrochloride（PAH） with human serum albumin（HSA） is of great significance in understanding the pharmacokinetic and pharmacodynamic mechanisms of the drug. Multi-spectroscopic techniques...Interaction of procainamide hydrochloride（PAH） with human serum albumin（HSA） is of great significance in understanding the pharmacokinetic and pharmacodynamic mechanisms of the drug. Multi-spectroscopic techniques were used to investigate the binding mode of PAH to HSA and results revealed the presence of static type of quenching mechanism. The number of binding sites, binding constants and thermodynamic parameters were calculated. The results showed a spontaneous binding of PAH to HSA and hydrophobic interactions played a major role. In addition, the distance between PAH and the Trp–214 was estimated employing the F？rster＇s theory. Site marker competitive experiments indicated that the binding of PAH to HSA primarily took place in subdomain IIA（Sudlow＇s site I）. The influence of interference of some common metal ions on the binding of PAH to HSA was studied. Synchronous fluorescence spectra（SFS）, 3D fluorescence spectra and circular dichroism（CD） results indicated the conformational changes in the structure of HSA.展开更多
AIM： To investigate the possible involvement of 25-hydroxyvitamin D3-1cx-hydroxylase [1α-25（OH）2D3] in butyrate-induced differentiation in human intestinal cell line Caco-2 cells. METHODS： Caco-2 cells were incub...AIM： To investigate the possible involvement of 25-hydroxyvitamin D3-1cx-hydroxylase [1α-25（OH）2D3] in butyrate-induced differentiation in human intestinal cell line Caco-2 cells. METHODS： Caco-2 cells were incubated either with 3 mmol/L butyrate and 1 umol/L 25（OH）2D3 or with 1 umol/L 1α-25（OH）2D3 for various time intervals ranging from 0 to 72 h. Additionally, cells were co-incubated with butyrate and either 25（OH）2D3 or 1α-25（OH）2D3. 1α-25（OH）2D3 mRNA was determined semi-quantitatively using the fluorescent dye PicoGreen. Immunoblotting was used for the detection of 1α-25（OH）2D3 protein. Finally, enzymatic activity was measured by ELISA. RESULTS： Both butyrate and 1α-25（OH）2D3 stimulated differentiation of Caco-2 cells after a 48 h incubation period, while 25（OH）2D3 had no impact on cell differentiation. Synergistic effects on differentiation were observed when cells were co-incubated with butyrate and vitamin D metabolite. Butyrate transiently upregulated 1α-25（OH）2D3 mRNA followed by a timely delayed protein upregulation. Coincidently, enzymatic activity was enhanced significantly. The induction of the enzyme allowed for comparable differentiating effects of both vitamin D metabolites. CONCLUSION： Our experimental data provide a further mechanism for the involvement of the vitamin D signaling pathway in colonic epithelial cell differentiation by butyrate. The enhancement of 1α-25（OH）2D3 followed by antiproliferative effects of the vitamin D prohormone in the Caco-2 cell line suggest that 25（OH）2D3 in combination with butyrate may offer a new therapeutic approach forthe treatment of colon cancer.展开更多
25-Hydroxycholesterol, which is an important intermediate for the synthesis of 25-hvdroxyvitamin D_3 and 1α, 25-dihydroxyvitamin D_3, was prepared efficiently from hyodeoxycholic acid in 31% overall yield in seven st...25-Hydroxycholesterol, which is an important intermediate for the synthesis of 25-hvdroxyvitamin D_3 and 1α, 25-dihydroxyvitamin D_3, was prepared efficiently from hyodeoxycholic acid in 31% overall yield in seven steps.展开更多
Objective: To study the correlation of late-pregnancy serum 25-OH-VitD3 content with maternal endothelial injury and placental apoptosis in pregnant women with preeclampsia (PE). Methods: PE pregnant women and healthy...Objective: To study the correlation of late-pregnancy serum 25-OH-VitD3 content with maternal endothelial injury and placental apoptosis in pregnant women with preeclampsia (PE). Methods: PE pregnant women and healthy pregnant women who gave birth in the First People's Hospital of Yichang between June 2014 and February 2017 were selected as PE group and control group respectively. At 32 weeks of gestation and before delivery, the serum was collected respectively to determine the contents of 25-OH-VitD3 and endothelial lesion markers;after delivery, the placenta was collected to determine the expression of apoptosis molecules. Results: 25-OH-VitD3 contents in serum as well as XIAP and Survivin mRNA expression in placental tissue of PE group were significantly lower than those of control group while sEng, sFlt-1, IFI16 and tTG contents in serum as well as AP-2 , Smac and PTEN mRNA expression in placental tissue were significantly higher than those of control group;sEng, sFlt-1, IFI16 and tTG contents in serum as well as AP-2 , Smac and PTEN mRNA expression in placental tissue of PE pregnant women with low 25-OH-VitD3 content were significantly higher than those of PE pregnant women with high 25-OH-VitD3 content while XIAP and Survivin mRNA expression were significantly lower than those of PE pregnant women with high 25-OH-VitD3 content. Conclusions: The decline of late-pregnancy serum 25-OH-VitD3 content can aggravate the maternal endothelial injury and placental apoptosis in pregnant women with PE.展开更多
目的:探讨妊娠早、中期妇女血清25-羟维生素D-3(25-OHD-3)与妊娠期糖尿病(GDM)的关系。方法:随机选取2018年1月至2021年12月在本院进行常规孕期检查的产妇作为研究对象,孕8~12周检测其空腹血糖(FBG)及糖化血红蛋白(HbAlc)水平,孕13~15...目的:探讨妊娠早、中期妇女血清25-羟维生素D-3(25-OHD-3)与妊娠期糖尿病(GDM)的关系。方法:随机选取2018年1月至2021年12月在本院进行常规孕期检查的产妇作为研究对象,孕8~12周检测其空腹血糖(FBG)及糖化血红蛋白(HbAlc)水平,孕13~15周检测其血清25-OHD-3水平,孕24~28周行75 g口服葡萄糖耐量试验(OGTT),包括FBG、餐后1 h血糖(1 h PBG)、餐后2 h血糖(2 h PBG),并在空腹、服糖水后1 h和2 h均检测血清25-OHD-3。根据OGTT筛查结果,将其分为GDM组(n=100)及对照组(n=100)。比较2组孕早期FBG、HbAlc,孕中期血清25-OHD-3及孕中后期空腹、服糖水后1、2 h的血糖及25-OHD-3等指标的差异,并分析血清25-OHD-3水平与GDM发病的关系。结果:GDM组孕早期FBG、HbAlc及OGTT中FBG、1 h PBG、2 h PBG均高于对照组(P<0.05);GDM组血清25-OHD-3水平低于对照组,差异具有统计学意义(P<0.05);两组孕妇存在孕期血清25-OHD-3不足且缺乏的情况,血清25-OHD-3指标GDM组充足率为6.00%,对照组为7.00%;GDM组不足率为30.00%,对照组为32.00%;GDM组缺乏率为64.00%,对照组为61.00%。综合比较显示,两组血清25-OHD-3充足率、不足率及缺乏率差异无统计学意义(P>0.05);GDM组中FBG正常组的25-OHD-3水平显著高于FBG异常组(P<0.05);而服糖后1 h及2 h,血糖正常组及异常组的25-OHD-3水平比较差异无统计学意义(P>0.05)。结论:随着孕龄的增加,孕妇血清25-OHD-3水平逐渐下降,而妊娠中期较低的血清25-OHD-3水平与GDM的发生存在相关性,增加了GDM发生的风险,对于高危人群需要加强早期监测,积极补充维生素D,以预防及减少GDM的发生。展开更多
AIM: To investigate the relationship between serum vitamin D3 levels and liver fibrosis or inflammation in treatment-naive Chinese patients with chronic hepatitis C(CHC).METHODS: From July 2010 to June 2011, we enroll...AIM: To investigate the relationship between serum vitamin D3 levels and liver fibrosis or inflammation in treatment-naive Chinese patients with chronic hepatitis C(CHC).METHODS: From July 2010 to June 2011, we enrolled 122 CHC patients and 11 healthy controls from Dingxicity, Gansu Province, China. The patients were infected with Hepatitis C virus(HCV) during blood cell retransfusion following plasma donation in 1992-1995, and had never received antiviral treatment. At present, all the patients except two underwent liver biopsy with ultrasound guidance. The Scheuer Scoring System was used to evaluate hepatic inflammation and the Metavir Scoring System was used to evaluate hepatic fibrosis. Twelve-hour overnight fasting blood samples were collected in the morning of the day of biopsy. Serum levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, cholineste rase, prothrom binactivity, albumin, γ-glutamyl transpeptidase, hemoglobin, calcium and phosphorus were determined. Serum HCV RNA levels were measured by real-time PCR. Serum levels of 25-hydroxyvitamin D3 [25(OH)D3] and 24,25-dihydroxyvitamin D3 [24,25(OH)2D3] were measured by high-performance liquid chromatography tandem mass spectrometry.R E S U LT S : Serumlevels of 2 5(OH) D3 but not 24,25(OH)2D3 were significantly lower in CHC patients than in control subjects. Serum 25(OH)D3 levels did not correlate with liver fibrosis, inflammation, patient age, or levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, prothrombin activity, cholinesterase or HCV RNA. However, serum 25(OH)D3 levels did correlate with serum 24,25(OH)2D3 levels. Serum 25(OH)D3 and 24,25(OH)2D3 levels, and the 25(OH)D3/24,25(OH)2D3 ratio, have no difference among the fibrosis stages or inflammation grades.CONCLUSION: We found that serum levels of 25(OH)D3 and its degradation metabolite 24,25(OH)2D3 did not correlate with liver fibrosis in treatment-naive Chinese patient with CHC.展开更多
Objective:To study the expression and significance of ECP, 25-(OH)D3 and M2 receptors in children with acute attack of asthma.Methods: Seventy children with bronchial asthma who first visited our hospital from Septemb...Objective:To study the expression and significance of ECP, 25-(OH)D3 and M2 receptors in children with acute attack of asthma.Methods: Seventy children with bronchial asthma who first visited our hospital from September 2016 to September 2018 were divided into chronic persistence group, remission group and acute attack group. Thirty healthy children who underwent physical examination in our hospital were selected and divided into control group. The levels of ECP, 25-(OH) D3 and M2 receptors were analyzed by ELISA, and Pearson correlation analysis was performed.Results: Compared with the control group, the levels of ECP and M2 receptors in chronic persistence group, remission group and acute attack group increased, while the levels of 25-(OH)D3 decreased, with statistical difference (P<0.05). The levels of ECP and M2 receptors in acute attack group were higher than those in chronic persistence group, and the levels of 25-(OH)D3 were lower than those in chronic persistence group (P<0.05). The levels of ECP and M2 receptors in acute attack group were higher than those in remission group, and the levels of 25-(OH)D3 were lower than those in remission group (P<0.05). Compared with mild children, the levels of ECP and M2 receptors increased and 25-(OH)D3 decreased in moderate and severe children (P<0.05). Compared with moderate children, the levels of ECP and M2 receptors increased and 25-(OH)D3 decreased in severe children (P<0.05).There was a negative correlation between ECP and 25-(OH)D3 (r=-0.380, P=0.038);a negative correlation between 25-(OH)D3 and M2 receptor (r=-0.448,P=0.013);and a positive correlation between ECP and M2 receptor (r=0.450,P=0.013).Conclusions:The expression of ECP and M2 receptors increased during the acute attack of bronchial asthma in children, while the expression of 25-hydroxyvitamin D3 decreased during the acute attack of bronchial asthma in children. The correlation among ECP, 25-(OH) D3 and M2 receptors is significant in the clinical diagnosis of acute attack of bronchial asthma in children.展开更多
Japan has the highest proportion of older adults and so-called “Super-aged society” in the world. These results suggested that the prevalence of both cognitive and physical functional impairment increases with age. ...Japan has the highest proportion of older adults and so-called “Super-aged society” in the world. These results suggested that the prevalence of both cognitive and physical functional impairment increases with age. Therefore, the present study was designed to investigate the effect of vitamin D3 supplementation in cognitive and physical functional impairment in healthy older adults in Japan. We enrolled 7 Japanese male (age: 76.0 ± 8.7) and 5 female (age: 78.3 ± 9.3) in this study. The physical function of even a person getting on a wheelchair could be tested in all subjects. Treatment group (n = 7) consisted of a 500 IU/day vitamin D3 corresponding to twice of daily requirement for 6 months. Blood was collected by venipuncture and the serum 25-hydroxy vitamin D (25 OHD), 1,25-hydroxy vitamin D (1,25 OHD), Ca and PTH concentration were measured. The Mini-Mental State Examination and the Montreal Cognitive Assessment-Japanese version were used for the cognitive function test. Physical function was measured objectively using the Timed Up and Go (TUG), 4-mgait speed test (4 MGS), 5-repetition sit-to-stand (5 STS), 30-second chair stand test (CS-30) and measurement of upper grip strength. Our results show that a 6-month intake of vitamin D3 increased serum 25 OHD within the insufficiency and sufficiency levels without changing 1,25 OHD, Ca and PTH concentration. And it significantly decreased 4 MGS without changing TUG, 5 STS, CS-30, upper grip strength and cognitive function. Serum 25 OHD cut-off values for skeletal muscle index, grip strength and 4 MGS in diagnosis of sarcopenia are 18.6 - 23.4 ng/mL. These findings suggest that serum 25 OHD levels (23.4 ng/mL) might improve moving capacity, lower limb muscle strength, and physical balance functional impairment.展开更多
Background: Data on the serum vitamin D3 levels of vernal keratoconjunctivitis (VKC) children are not known well in Central Africa. Children with vernal keratoconjunctivitis are less exposed to sunlight. There is no s...Background: Data on the serum vitamin D3 levels of vernal keratoconjunctivitis (VKC) children are not known well in Central Africa. Children with vernal keratoconjunctivitis are less exposed to sunlight. There is no study that shows the breadth of the serum vitamin D3 levels of vernal keratoconjunctivitis children and normal in our setting. Purpose: This study aimed to compare the serum 25-hydroxy vitamin D [25(OH)D3] levels of children with and without VKC. Methods: An observational, case-control study was performed from September 2019 to February 2020 in the Ophthalmology Department and the Clinical Biology Department of the University Hospital of Kinshasa. The case group was recruited consecutively in the consultation while the control group was enrolled in the nearest Rehoboth school complex using a systematic sample method (k = 4) on the presence register. Both cases and controls underwent the routine ophthalmological examination of the anterior segment and serum vitamin D3 levels were measured in both cases and controls using Mindray Chimic-Luminescence (CL)-1200i. Data were entered in Microsoft Sheets and analyzed using SPSS version 20.0. The Mann-Whitney-U test (M-U), and the t-Student test were used respectively to compare the mean serum vitamin D3 levels in both cases and controls. The correlation of Spearman (rho) was used to determine the association between Body Mass Index and serum vitamin D3 levels in the case group. Results: A total of 75 children with VKC (mean age 7.8 ± 4.4 years) and 75 children without VKC non-atopic control group (mean age 7.9 ± 4.3 years) were enrolled in this study. The vitamin D3 deficiency was more frequent among the case group than in the control group (40% vs 8%, p = 0.0001). Vitamin D3 insufficiency was more frequent in the case group than in the control group (33.3% vs 18.6%, p = 0.04). The mean serum vitamin 25(OH)D3 levels were statistically lower in children with VKC compared to those without (25.5 ± 8.7 ng/ml and 44.3 ± 18.5 ng/ml, p = 0.0001). Time spent outdoors during daylight by children with VKC was statistically lower than children without VKC (1.59 ± 0.71 hours and 2.28 ± 1.08 hours, respectively) (p = 0.0001). Body Mass Index and serum vitamin D3 levels in VKC children showed a negative correlation statistically significant (Spearman, rho = -0.452, p = 0.0001). Conclusion: This study showed statistically values of serum vitamin D3 levels in VKC children lower than in children without VKC.展开更多
Background Human adipose tissue-derived stromal cells （hADSCs） can be induced to differentiate along an osteoblastic lineage under stimulation of dexamethasone （DEX）. Recent studies, however, have questioned the e...Background Human adipose tissue-derived stromal cells （hADSCs） can be induced to differentiate along an osteoblastic lineage under stimulation of dexamethasone （DEX）. Recent studies, however, have questioned the efficacy of glucocorticoids such as DEX in mediating the osteogenesis process of skeletal progenitor cells and processed lipoaspirate cells. Is it possible to find a substitute for DEX？ Therefore, this study was designed to investigate osteogenic capacity and regulating mechanisms for osteoblastic differentiation of hADSCs by comparing osteogenic media （OM） containing either 1, 25-dihydroxyvitamin D3 （VD） or DEX and determine if VD was an ideal substitute for DEX as an induction agent for the osteogenesis of hADSCs. Methods Osteogenic differentiation of hADSCs was induced by osteogenic medium （OM） containing either 10 nmol/L VD or 100 nmol/L DEX. Differentiation of hADSCs into osteoblastic lineage was identified by alkaline phosphatase （ALP） staining, von Kossa staining, and reverse transcription-polymerase chain reaction assays for mRNA expression of osteogenesis-related genes such as type Ⅰ collagen （COL Ⅰ）, bone sialoprotein （BSP）, osteocalcin （OC）, bone morphogenetic protein （BMP）-2, BMP-4, BMP-6, BMP-7, runt-related transcription factor 2/core binding factor α1 （Runx2/Cbfal）, osterix （Osx）, and LIM mineralization protein- 1 （LMP- 1）. Results von Kossa staining revealed that the differentiated cells induced by both VD and DEX were mineralized in vitro. They also expressed osteoblast-related markers, such as ALP, COL Ⅰ, BSP, and OC. Runx2/Cbfal, Osx, BMP-6, and LMP-1 were upregulated during VD and DEX-induced hADSC osteoblastic differentiation, but BMP-4, BMP-7 were not. BMP-2 was only expressed in VD-induced differentiated cells. Conclusions VD or DEX-induced hADSCs differentiate toward the osteoblastic lineage in vitro. Runx2/Cbfal, Osx, BMP-2, BMP-6, and LMP-1 are involved in regulating osteoblastic differentiation of hADSCs, but BMP-4, BMP-7 are not. VD, but not DEX, induces expression of BMP-2 during osteogenic induction of hADSCs. VD is an ideal substitute for DEX for osteogenic induction of hADSCs.展开更多
Dietary supplementation with 25-hydroxyvitamin D_(3)(25OHD_(3)),as an alternative source of vitamin D,is becoming increasingly popular due to its commercialization and more efficient absorbability.The addition of 25OH...Dietary supplementation with 25-hydroxyvitamin D_(3)(25OHD_(3)),as an alternative source of vitamin D,is becoming increasingly popular due to its commercialization and more efficient absorbability.The addition of 25OHD_(3)rather than its precursor vitamin D_(3)can circumvent the 25-hydroxylation reaction in the liver,indicating that supplementation of 25OHD_(3)can rapidly improve the circulating vitamin D status of animals.Emerging experiments have reported that maternal 25OHD_(3)supplementation could increase sow performances and birth outcomes and promote circulating vitamin D status of sows and their offspring.Increased milk fat content was observed in many experiments;however,others demonstrated that adding 25OHD_(3)to lactating sow diets increased the contents of milk protein and lactose.Although an inconsistency between the results of different experiments exists,these studies suggested that maternal 25OHD_(3)supplementation could alter milk composition via its effects on the mammary gland.Previous studies have demonstrated that adding 25OHD_(3)to sow diets could improve the mRNA expressions of insulin-induced gene 1(INSIG1)and sterol regulatory element-binding protein 1(SREBP1)in the mammary gland cells from milk and increase the mRNA expressions of acetyl-CoA carboxylaseα(ACCα)and fatty acid synthase(FAS)in the mammary gland tissue.Maternal 25OHD_(3)supplementation promotes skeletal muscle development of piglets before and after parturition,and improves bone properties including bone density and bone breaking force in lactating sows and their piglets.Interestingly,25OHD_(3)supplementation in sow diets could improve neonatal bone development via regulation of milk fatty acid composition related to bone metabolism and mineralization.In this review,we also discuss the effects of adding 25OHD_(3)to sow diets on the gut bacterial metabolites of suckling piglets,and propose that butyrate production may be associated with bone health.Therefore,to better understand the nutritional functions of maternal 25OHD_(3)supplementation,this paper reviews advances in the studies of 25OHD_(3)for sow nutrition and provides references for practical application.展开更多
文摘BACKGROUND Gestational diabetes mellitus(GDM)is a concern due to its rapid increase in incidence in recent years.AIM To investigate the correlation and predictive value of serum pregnancyassociated plasma protein A(PAPP-A),triglyceride(TG),and 25-hydroxyvitamin D[25-(OH)D]with GDM in early pregnancy.METHODS A total of 99 patients in early pregnancy admitted to Peking University International Hospital from November 2015 to September 2017 were included,and underwent a fasting glucose test and oral glucose tolerance test screening at 24-28 wk of pregnancy.Of these cases with GDM,51 were assigned to group A and the remaining 48 cases without GDM were enrolled in group B.Serum PAPP-A,TG and 25-(OH)D in the two groups were compared and their correlation with blood sugar was analyzed.In addition,their diagnostic value in GDM was determined using receiver operating characteristic(ROC)curve analysis.RESULTS Group A had markedly lower serum PAPP-A and 25-(OH)D levels and a significantly higher serum TG level than group B,with statistical significance(P<0.05).Furthermore,Pearson analysis identified that PAPP-A and 25-(OH)D levels were negatively correlated with fasting blood glucose(FBG)levels(r=-0.605,P<0.001),(r=-0.597,P<0.001),while TG and FBG levels were positively correlated(r=0.628,P<0.001).The sensitivity,specificity,area under the curve(AUC)and optimal cut-off value of serum PAPP-A level in the diagnosis of GDM were 72.55%,82.35%,0.861 and 16.340,respectively,while the sensitivity of TG in diagnosing GDM was 86.27%,the specificity was 66.67%,the AUC was 0.813,with an optimal cut-off value of 1.796.The corresponding sensitivity,specificity,AUC and optimal cut-off value of serum 25-(OH)D were 64.71%,70.59%,0.721 and 23.140,respectively.Moreover,multivariate logistic regression analysis revealed that FBG,vascular endothelial growth factor,Flt-1,serum PAPP-A,TG,and 25-(OH)D were related risk factors leading to GDM in patients.CONCLUSION Serum PAPP-A,TG,and 25-(OH)D levels are all correlated with blood glucose changes in GDM,and are independent factors affecting the occurrence of GDM and have certain value in the diagnosis of GDM.
文摘We enrolled 23 Japanese men (age: 76.0 ± 8.7) and 17 women (age: 78.3 ± 9.3) in this study. The physical function of even a person getting on a wheelchair could be tested in all subjects. Blood was collected by venipuncture and the serum 1, 25-hydroxy vitamin D (1, 25OHD) concentration was measured. The Mini-Mental State Examination (MMSE) and the Montreal Cognitive Assessment-Japanese version (MoCA-J) was used for the cognitive function test. Physical function was measured objectively using the Timed UP and Go (TUG) and 4-m walking test (4MWS). A significant positive correlation was found between serum 1, 25OHD and MMSE or MoCA-J. It is expected that an elderly person can maintain a mean serum 1, 25OHD level of about 100 pg/mL for preventing early cognitive disorder. In the present study, a significant positive correlation was found between urinary 25-hydroxy vitamin D (25OHD)/creatinine and MMSE or MoCA-J. Our results showed that urinary 25OHD might be a useful biomarker for predicting cognitive disorder. There was a significant negative correlation between serum 1, 25OHD and TUG or 4MWS. These findings suggest that serum 1, 25OHD levels might serve as a useful index to improve cognitive and physical functional impairment.
文摘Interaction of procainamide hydrochloride（PAH） with human serum albumin（HSA） is of great significance in understanding the pharmacokinetic and pharmacodynamic mechanisms of the drug. Multi-spectroscopic techniques were used to investigate the binding mode of PAH to HSA and results revealed the presence of static type of quenching mechanism. The number of binding sites, binding constants and thermodynamic parameters were calculated. The results showed a spontaneous binding of PAH to HSA and hydrophobic interactions played a major role. In addition, the distance between PAH and the Trp–214 was estimated employing the F？rster＇s theory. Site marker competitive experiments indicated that the binding of PAH to HSA primarily took place in subdomain IIA（Sudlow＇s site I）. The influence of interference of some common metal ions on the binding of PAH to HSA was studied. Synchronous fluorescence spectra（SFS）, 3D fluorescence spectra and circular dichroism（CD） results indicated the conformational changes in the structure of HSA.
基金Supported by the Else Kroner-Fresenius Foundation, Bad Homburg, Germany
文摘AIM： To investigate the possible involvement of 25-hydroxyvitamin D3-1cx-hydroxylase [1α-25（OH）2D3] in butyrate-induced differentiation in human intestinal cell line Caco-2 cells. METHODS： Caco-2 cells were incubated either with 3 mmol/L butyrate and 1 umol/L 25（OH）2D3 or with 1 umol/L 1α-25（OH）2D3 for various time intervals ranging from 0 to 72 h. Additionally, cells were co-incubated with butyrate and either 25（OH）2D3 or 1α-25（OH）2D3. 1α-25（OH）2D3 mRNA was determined semi-quantitatively using the fluorescent dye PicoGreen. Immunoblotting was used for the detection of 1α-25（OH）2D3 protein. Finally, enzymatic activity was measured by ELISA. RESULTS： Both butyrate and 1α-25（OH）2D3 stimulated differentiation of Caco-2 cells after a 48 h incubation period, while 25（OH）2D3 had no impact on cell differentiation. Synergistic effects on differentiation were observed when cells were co-incubated with butyrate and vitamin D metabolite. Butyrate transiently upregulated 1α-25（OH）2D3 mRNA followed by a timely delayed protein upregulation. Coincidently, enzymatic activity was enhanced significantly. The induction of the enzyme allowed for comparable differentiating effects of both vitamin D metabolites. CONCLUSION： Our experimental data provide a further mechanism for the involvement of the vitamin D signaling pathway in colonic epithelial cell differentiation by butyrate. The enhancement of 1α-25（OH）2D3 followed by antiproliferative effects of the vitamin D prohormone in the Caco-2 cell line suggest that 25（OH）2D3 in combination with butyrate may offer a new therapeutic approach forthe treatment of colon cancer.
文摘25-Hydroxycholesterol, which is an important intermediate for the synthesis of 25-hvdroxyvitamin D_3 and 1α, 25-dihydroxyvitamin D_3, was prepared efficiently from hyodeoxycholic acid in 31% overall yield in seven steps.
文摘Objective: To study the correlation of late-pregnancy serum 25-OH-VitD3 content with maternal endothelial injury and placental apoptosis in pregnant women with preeclampsia (PE). Methods: PE pregnant women and healthy pregnant women who gave birth in the First People's Hospital of Yichang between June 2014 and February 2017 were selected as PE group and control group respectively. At 32 weeks of gestation and before delivery, the serum was collected respectively to determine the contents of 25-OH-VitD3 and endothelial lesion markers;after delivery, the placenta was collected to determine the expression of apoptosis molecules. Results: 25-OH-VitD3 contents in serum as well as XIAP and Survivin mRNA expression in placental tissue of PE group were significantly lower than those of control group while sEng, sFlt-1, IFI16 and tTG contents in serum as well as AP-2 , Smac and PTEN mRNA expression in placental tissue were significantly higher than those of control group;sEng, sFlt-1, IFI16 and tTG contents in serum as well as AP-2 , Smac and PTEN mRNA expression in placental tissue of PE pregnant women with low 25-OH-VitD3 content were significantly higher than those of PE pregnant women with high 25-OH-VitD3 content while XIAP and Survivin mRNA expression were significantly lower than those of PE pregnant women with high 25-OH-VitD3 content. Conclusions: The decline of late-pregnancy serum 25-OH-VitD3 content can aggravate the maternal endothelial injury and placental apoptosis in pregnant women with PE.
文摘目的:探讨妊娠早、中期妇女血清25-羟维生素D-3(25-OHD-3)与妊娠期糖尿病(GDM)的关系。方法:随机选取2018年1月至2021年12月在本院进行常规孕期检查的产妇作为研究对象,孕8~12周检测其空腹血糖(FBG)及糖化血红蛋白(HbAlc)水平,孕13~15周检测其血清25-OHD-3水平,孕24~28周行75 g口服葡萄糖耐量试验(OGTT),包括FBG、餐后1 h血糖(1 h PBG)、餐后2 h血糖(2 h PBG),并在空腹、服糖水后1 h和2 h均检测血清25-OHD-3。根据OGTT筛查结果,将其分为GDM组(n=100)及对照组(n=100)。比较2组孕早期FBG、HbAlc,孕中期血清25-OHD-3及孕中后期空腹、服糖水后1、2 h的血糖及25-OHD-3等指标的差异,并分析血清25-OHD-3水平与GDM发病的关系。结果:GDM组孕早期FBG、HbAlc及OGTT中FBG、1 h PBG、2 h PBG均高于对照组(P<0.05);GDM组血清25-OHD-3水平低于对照组,差异具有统计学意义(P<0.05);两组孕妇存在孕期血清25-OHD-3不足且缺乏的情况,血清25-OHD-3指标GDM组充足率为6.00%,对照组为7.00%;GDM组不足率为30.00%,对照组为32.00%;GDM组缺乏率为64.00%,对照组为61.00%。综合比较显示,两组血清25-OHD-3充足率、不足率及缺乏率差异无统计学意义(P>0.05);GDM组中FBG正常组的25-OHD-3水平显著高于FBG异常组(P<0.05);而服糖后1 h及2 h,血糖正常组及异常组的25-OHD-3水平比较差异无统计学意义(P>0.05)。结论:随着孕龄的增加,孕妇血清25-OHD-3水平逐渐下降,而妊娠中期较低的血清25-OHD-3水平与GDM的发生存在相关性,增加了GDM发生的风险,对于高危人群需要加强早期监测,积极补充维生素D,以预防及减少GDM的发生。
基金Grants from High technical personnel training item from Beijing Health System,No.2011-3-083,No.2013-3-071Beijing Municipal Science&Technology Commission,No.Z131107002213019,No.Z151100004015066+4 种基金Base to Clinical Scientific Research Cooperation fund of Capital Medical University No.15JL67National Science and Technology Key Project on"Major Infectious Diseases such as HIV/AIDS,Viral Hepatitis Prevention and Treatment",No.2012ZX10002004-006,No.2012ZX10004904-003-001,and No.2013ZX10002002-006Beijing Municipal Administration of Hospitals Clinical medicine Development of special funding support,No.XM201308National Key Subject Construction Project,NO.WJWYA-2014-002Scientific research base construction-the major infectious diseases prevention and control of collaborative innovation center,No.115215
文摘AIM: To investigate the relationship between serum vitamin D3 levels and liver fibrosis or inflammation in treatment-naive Chinese patients with chronic hepatitis C(CHC).METHODS: From July 2010 to June 2011, we enrolled 122 CHC patients and 11 healthy controls from Dingxicity, Gansu Province, China. The patients were infected with Hepatitis C virus(HCV) during blood cell retransfusion following plasma donation in 1992-1995, and had never received antiviral treatment. At present, all the patients except two underwent liver biopsy with ultrasound guidance. The Scheuer Scoring System was used to evaluate hepatic inflammation and the Metavir Scoring System was used to evaluate hepatic fibrosis. Twelve-hour overnight fasting blood samples were collected in the morning of the day of biopsy. Serum levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, cholineste rase, prothrom binactivity, albumin, γ-glutamyl transpeptidase, hemoglobin, calcium and phosphorus were determined. Serum HCV RNA levels were measured by real-time PCR. Serum levels of 25-hydroxyvitamin D3 [25(OH)D3] and 24,25-dihydroxyvitamin D3 [24,25(OH)2D3] were measured by high-performance liquid chromatography tandem mass spectrometry.R E S U LT S : Serumlevels of 2 5(OH) D3 but not 24,25(OH)2D3 were significantly lower in CHC patients than in control subjects. Serum 25(OH)D3 levels did not correlate with liver fibrosis, inflammation, patient age, or levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, prothrombin activity, cholinesterase or HCV RNA. However, serum 25(OH)D3 levels did correlate with serum 24,25(OH)2D3 levels. Serum 25(OH)D3 and 24,25(OH)2D3 levels, and the 25(OH)D3/24,25(OH)2D3 ratio, have no difference among the fibrosis stages or inflammation grades.CONCLUSION: We found that serum levels of 25(OH)D3 and its degradation metabolite 24,25(OH)2D3 did not correlate with liver fibrosis in treatment-naive Chinese patient with CHC.
基金Key projects funded by the science and technology bureau of Chenzhou city(No.CZ2013065).
文摘Objective:To study the expression and significance of ECP, 25-(OH)D3 and M2 receptors in children with acute attack of asthma.Methods: Seventy children with bronchial asthma who first visited our hospital from September 2016 to September 2018 were divided into chronic persistence group, remission group and acute attack group. Thirty healthy children who underwent physical examination in our hospital were selected and divided into control group. The levels of ECP, 25-(OH) D3 and M2 receptors were analyzed by ELISA, and Pearson correlation analysis was performed.Results: Compared with the control group, the levels of ECP and M2 receptors in chronic persistence group, remission group and acute attack group increased, while the levels of 25-(OH)D3 decreased, with statistical difference (P<0.05). The levels of ECP and M2 receptors in acute attack group were higher than those in chronic persistence group, and the levels of 25-(OH)D3 were lower than those in chronic persistence group (P<0.05). The levels of ECP and M2 receptors in acute attack group were higher than those in remission group, and the levels of 25-(OH)D3 were lower than those in remission group (P<0.05). Compared with mild children, the levels of ECP and M2 receptors increased and 25-(OH)D3 decreased in moderate and severe children (P<0.05). Compared with moderate children, the levels of ECP and M2 receptors increased and 25-(OH)D3 decreased in severe children (P<0.05).There was a negative correlation between ECP and 25-(OH)D3 (r=-0.380, P=0.038);a negative correlation between 25-(OH)D3 and M2 receptor (r=-0.448,P=0.013);and a positive correlation between ECP and M2 receptor (r=0.450,P=0.013).Conclusions:The expression of ECP and M2 receptors increased during the acute attack of bronchial asthma in children, while the expression of 25-hydroxyvitamin D3 decreased during the acute attack of bronchial asthma in children. The correlation among ECP, 25-(OH) D3 and M2 receptors is significant in the clinical diagnosis of acute attack of bronchial asthma in children.
文摘Japan has the highest proportion of older adults and so-called “Super-aged society” in the world. These results suggested that the prevalence of both cognitive and physical functional impairment increases with age. Therefore, the present study was designed to investigate the effect of vitamin D3 supplementation in cognitive and physical functional impairment in healthy older adults in Japan. We enrolled 7 Japanese male (age: 76.0 ± 8.7) and 5 female (age: 78.3 ± 9.3) in this study. The physical function of even a person getting on a wheelchair could be tested in all subjects. Treatment group (n = 7) consisted of a 500 IU/day vitamin D3 corresponding to twice of daily requirement for 6 months. Blood was collected by venipuncture and the serum 25-hydroxy vitamin D (25 OHD), 1,25-hydroxy vitamin D (1,25 OHD), Ca and PTH concentration were measured. The Mini-Mental State Examination and the Montreal Cognitive Assessment-Japanese version were used for the cognitive function test. Physical function was measured objectively using the Timed Up and Go (TUG), 4-mgait speed test (4 MGS), 5-repetition sit-to-stand (5 STS), 30-second chair stand test (CS-30) and measurement of upper grip strength. Our results show that a 6-month intake of vitamin D3 increased serum 25 OHD within the insufficiency and sufficiency levels without changing 1,25 OHD, Ca and PTH concentration. And it significantly decreased 4 MGS without changing TUG, 5 STS, CS-30, upper grip strength and cognitive function. Serum 25 OHD cut-off values for skeletal muscle index, grip strength and 4 MGS in diagnosis of sarcopenia are 18.6 - 23.4 ng/mL. These findings suggest that serum 25 OHD levels (23.4 ng/mL) might improve moving capacity, lower limb muscle strength, and physical balance functional impairment.
文摘Background: Data on the serum vitamin D3 levels of vernal keratoconjunctivitis (VKC) children are not known well in Central Africa. Children with vernal keratoconjunctivitis are less exposed to sunlight. There is no study that shows the breadth of the serum vitamin D3 levels of vernal keratoconjunctivitis children and normal in our setting. Purpose: This study aimed to compare the serum 25-hydroxy vitamin D [25(OH)D3] levels of children with and without VKC. Methods: An observational, case-control study was performed from September 2019 to February 2020 in the Ophthalmology Department and the Clinical Biology Department of the University Hospital of Kinshasa. The case group was recruited consecutively in the consultation while the control group was enrolled in the nearest Rehoboth school complex using a systematic sample method (k = 4) on the presence register. Both cases and controls underwent the routine ophthalmological examination of the anterior segment and serum vitamin D3 levels were measured in both cases and controls using Mindray Chimic-Luminescence (CL)-1200i. Data were entered in Microsoft Sheets and analyzed using SPSS version 20.0. The Mann-Whitney-U test (M-U), and the t-Student test were used respectively to compare the mean serum vitamin D3 levels in both cases and controls. The correlation of Spearman (rho) was used to determine the association between Body Mass Index and serum vitamin D3 levels in the case group. Results: A total of 75 children with VKC (mean age 7.8 ± 4.4 years) and 75 children without VKC non-atopic control group (mean age 7.9 ± 4.3 years) were enrolled in this study. The vitamin D3 deficiency was more frequent among the case group than in the control group (40% vs 8%, p = 0.0001). Vitamin D3 insufficiency was more frequent in the case group than in the control group (33.3% vs 18.6%, p = 0.04). The mean serum vitamin 25(OH)D3 levels were statistically lower in children with VKC compared to those without (25.5 ± 8.7 ng/ml and 44.3 ± 18.5 ng/ml, p = 0.0001). Time spent outdoors during daylight by children with VKC was statistically lower than children without VKC (1.59 ± 0.71 hours and 2.28 ± 1.08 hours, respectively) (p = 0.0001). Body Mass Index and serum vitamin D3 levels in VKC children showed a negative correlation statistically significant (Spearman, rho = -0.452, p = 0.0001). Conclusion: This study showed statistically values of serum vitamin D3 levels in VKC children lower than in children without VKC.
基金This study was supported by a grant from the National Natural Science Foundation of China (No. 30200319).
文摘Background Human adipose tissue-derived stromal cells （hADSCs） can be induced to differentiate along an osteoblastic lineage under stimulation of dexamethasone （DEX）. Recent studies, however, have questioned the efficacy of glucocorticoids such as DEX in mediating the osteogenesis process of skeletal progenitor cells and processed lipoaspirate cells. Is it possible to find a substitute for DEX？ Therefore, this study was designed to investigate osteogenic capacity and regulating mechanisms for osteoblastic differentiation of hADSCs by comparing osteogenic media （OM） containing either 1, 25-dihydroxyvitamin D3 （VD） or DEX and determine if VD was an ideal substitute for DEX as an induction agent for the osteogenesis of hADSCs. Methods Osteogenic differentiation of hADSCs was induced by osteogenic medium （OM） containing either 10 nmol/L VD or 100 nmol/L DEX. Differentiation of hADSCs into osteoblastic lineage was identified by alkaline phosphatase （ALP） staining, von Kossa staining, and reverse transcription-polymerase chain reaction assays for mRNA expression of osteogenesis-related genes such as type Ⅰ collagen （COL Ⅰ）, bone sialoprotein （BSP）, osteocalcin （OC）, bone morphogenetic protein （BMP）-2, BMP-4, BMP-6, BMP-7, runt-related transcription factor 2/core binding factor α1 （Runx2/Cbfal）, osterix （Osx）, and LIM mineralization protein- 1 （LMP- 1）. Results von Kossa staining revealed that the differentiated cells induced by both VD and DEX were mineralized in vitro. They also expressed osteoblast-related markers, such as ALP, COL Ⅰ, BSP, and OC. Runx2/Cbfal, Osx, BMP-6, and LMP-1 were upregulated during VD and DEX-induced hADSC osteoblastic differentiation, but BMP-4, BMP-7 were not. BMP-2 was only expressed in VD-induced differentiated cells. Conclusions VD or DEX-induced hADSCs differentiate toward the osteoblastic lineage in vitro. Runx2/Cbfal, Osx, BMP-2, BMP-6, and LMP-1 are involved in regulating osteoblastic differentiation of hADSCs, but BMP-4, BMP-7 are not. VD, but not DEX, induces expression of BMP-2 during osteogenic induction of hADSCs. VD is an ideal substitute for DEX for osteogenic induction of hADSCs.
基金supported by the Beijing Municipal Natural Science Foundation(6202019)the National Natural Science Foundation of China(31772612)
文摘Dietary supplementation with 25-hydroxyvitamin D_(3)(25OHD_(3)),as an alternative source of vitamin D,is becoming increasingly popular due to its commercialization and more efficient absorbability.The addition of 25OHD_(3)rather than its precursor vitamin D_(3)can circumvent the 25-hydroxylation reaction in the liver,indicating that supplementation of 25OHD_(3)can rapidly improve the circulating vitamin D status of animals.Emerging experiments have reported that maternal 25OHD_(3)supplementation could increase sow performances and birth outcomes and promote circulating vitamin D status of sows and their offspring.Increased milk fat content was observed in many experiments;however,others demonstrated that adding 25OHD_(3)to lactating sow diets increased the contents of milk protein and lactose.Although an inconsistency between the results of different experiments exists,these studies suggested that maternal 25OHD_(3)supplementation could alter milk composition via its effects on the mammary gland.Previous studies have demonstrated that adding 25OHD_(3)to sow diets could improve the mRNA expressions of insulin-induced gene 1(INSIG1)and sterol regulatory element-binding protein 1(SREBP1)in the mammary gland cells from milk and increase the mRNA expressions of acetyl-CoA carboxylaseα(ACCα)and fatty acid synthase(FAS)in the mammary gland tissue.Maternal 25OHD_(3)supplementation promotes skeletal muscle development of piglets before and after parturition,and improves bone properties including bone density and bone breaking force in lactating sows and their piglets.Interestingly,25OHD_(3)supplementation in sow diets could improve neonatal bone development via regulation of milk fatty acid composition related to bone metabolism and mineralization.In this review,we also discuss the effects of adding 25OHD_(3)to sow diets on the gut bacterial metabolites of suckling piglets,and propose that butyrate production may be associated with bone health.Therefore,to better understand the nutritional functions of maternal 25OHD_(3)supplementation,this paper reviews advances in the studies of 25OHD_(3)for sow nutrition and provides references for practical application.
