OBJECTIVE To examine the possibility of human sodium iodide symporter （hNIS） protein expression in lung cancer cells. METHODS Human lung A549 cancer cells were thawed and cultured in vitro. The cells were divided in...OBJECTIVE To examine the possibility of human sodium iodide symporter （hNIS） protein expression in lung cancer cells. METHODS Human lung A549 cancer cells were thawed and cultured in vitro. The cells were divided into an experimental group transfected with a recombinant pcDNA3-hNIS plasmid and a control group transfected only with a pcDNA3 plasmid. The recombinant plasmid vector encoding the hNIS gene （pcDNA3-hNIS） was amplified, purified and identified. The hNIS gene was followed by DNA sequencing. A Western blot and an immunohistochemical assay were applied to detect the hNIS protein expression in the transfected human lung A549 cancer cells. RESULTS Restriction enzyme digestion and DNA sequencing results showed the size and direction of the inserted gene in the recombinant pcD- NA3-hNIS plasmid was correct. The Western blot method and immunohistochemical analysis showed a positive NIS protein expression in the experimental group. The NIS protein was detected mainly in the cell membranes showing a positive rate up to 70.6% with no expression of the NIS protein in the control group. There was a significant difference between two groups （P=0.000）. CONCLUSION The hNIS gene was transfected effectively into human lung A549 cancer cells mediated by Lipofectamine 2000, and was expressed with its protein in vitro.展开更多
萨默塞特.毛姆(Somerset Maugham)是英国小说家和剧作家,他的短篇小说《A Friend in Need》堪称揭示人性的多重性、复杂性的一篇佳作。以往的评论家和读者对这一短篇小说中人物人性的"残酷"性一面的理解多拘泥于传统,因而过...萨默塞特.毛姆(Somerset Maugham)是英国小说家和剧作家,他的短篇小说《A Friend in Need》堪称揭示人性的多重性、复杂性的一篇佳作。以往的评论家和读者对这一短篇小说中人物人性的"残酷"性一面的理解多拘泥于传统,因而过于肤浅。透过西方的基督教文化,从心理学角度解析这篇小说中人物罪行的根源,启发人们从一个新的视角看待人性,帮助揭开对人性复杂性的迷惘和困惑。展开更多
文摘OBJECTIVE To examine the possibility of human sodium iodide symporter （hNIS） protein expression in lung cancer cells. METHODS Human lung A549 cancer cells were thawed and cultured in vitro. The cells were divided into an experimental group transfected with a recombinant pcDNA3-hNIS plasmid and a control group transfected only with a pcDNA3 plasmid. The recombinant plasmid vector encoding the hNIS gene （pcDNA3-hNIS） was amplified, purified and identified. The hNIS gene was followed by DNA sequencing. A Western blot and an immunohistochemical assay were applied to detect the hNIS protein expression in the transfected human lung A549 cancer cells. RESULTS Restriction enzyme digestion and DNA sequencing results showed the size and direction of the inserted gene in the recombinant pcD- NA3-hNIS plasmid was correct. The Western blot method and immunohistochemical analysis showed a positive NIS protein expression in the experimental group. The NIS protein was detected mainly in the cell membranes showing a positive rate up to 70.6% with no expression of the NIS protein in the control group. There was a significant difference between two groups （P=0.000）. CONCLUSION The hNIS gene was transfected effectively into human lung A549 cancer cells mediated by Lipofectamine 2000, and was expressed with its protein in vitro.
文摘萨默塞特.毛姆(Somerset Maugham)是英国小说家和剧作家,他的短篇小说《A Friend in Need》堪称揭示人性的多重性、复杂性的一篇佳作。以往的评论家和读者对这一短篇小说中人物人性的"残酷"性一面的理解多拘泥于传统,因而过于肤浅。透过西方的基督教文化,从心理学角度解析这篇小说中人物罪行的根源,启发人们从一个新的视角看待人性,帮助揭开对人性复杂性的迷惘和困惑。
