HBsAg and HBV DNA in the sweat of HBsAg carriers were detected.Thepositivity rates were 26.1%(12/46)by RIA and 17.4%(8/45)by dot blot hybridization.No HBsAg and HBV DNA were found in 6 persons with negative serum HBsA...HBsAg and HBV DNA in the sweat of HBsAg carriers were detected.Thepositivity rates were 26.1%(12/46)by RIA and 17.4%(8/45)by dot blot hybridization.No HBsAg and HBV DNA were found in 6 persons with negative serum HBsAg.Viralparticles were observed in the sweat by means of immunoelectronic microscope.When se-rum HBeAg or HBV DNA were positive,the positivity rates of HBsAg and HBV DNAin sweat were higher than those with negative serum HBeAg and HBV DNA,and the dif-ference was significant.The results suggested that HBV may be excreted out ofbody through the sweat glands of serum HBsAg carriers,and contaminate environmentalobjects,which may play a role in contact transmission of hepatitis B.No relationshipswere found between anti-HBc,anti-HBe,titer of serum HBsAg and positivity rates ofHBsAg and HBV DNA in sweat.展开更多
This paper reports the loss of several major nutrients in the sweat from humanbody during working in hot environment,so as to put forward a rational scheme for sup-plying beverages and foods with appropriate nutrients...This paper reports the loss of several major nutrients in the sweat from humanbody during working in hot environment,so as to put forward a rational scheme for sup-plying beverages and foods with appropriate nutrients.Thirteen young male subjectsdressed in gas protection equipment of the whole body were ordered to march in a hotclimatic chamber（DB 34.2℃）at a speed of 5.4 km/h until they subjectively failed to bearfurther but to hold on still for 200 m.The results showed that their maximum tolerancetime was only 58.2±2.9 min,the mean body temperature（MBT）was 37.8±0.1 ℃,heartrate（HR）151.3±8.0 beat/min.Nevertheless,the amount of sweat was only 780.5±75.2 g.Thelevels of K,Na,Ca,Mg,Fe,Cu and Zn in the whole body-sweat of our series were12.3±1.8,113.0±7.0,0.8±0.13,0.9±0.20,0.027±0.004,0.005±0.001 and 0.035±0.002mmol/L,respectively.The concentrations of K and Zn in serum were decreased,and thelevel of Zn was also lower than the normal.The total amount of amino acids in thesweat was 10855±679 μmol/L,of which the amount of essential amino acids was 21.2%.The concentration of lysine was the highest（1038.5±90.7μmol/L）,and that of methioninethe lowest（49.8±4.0μmol/L）in the essential amino acids.In the non-essential aminoacids,the concentrations of serine and glycine were the highest,being 2774.3±179.0 and1572.1±83.9 μmol/L,respectively,and that of cystine the lowest,being 4.8±0.9μmol/L.The amount of vitamins B<sub>1</sub>,B<sub>2</sub> and C in the sweat were only 452±220,312±174 and3330±1200 μg/L,respectively.展开更多
Objective:This study was performed to explore the conversion of adipose-derived stem cells(ADSCs)into sweat gland-like cells for the purpose of sweat gland regeneration.Methods:ADSCs and human sweat gland(hSG)cells we...Objective:This study was performed to explore the conversion of adipose-derived stem cells(ADSCs)into sweat gland-like cells for the purpose of sweat gland regeneration.Methods:ADSCs and human sweat gland(hSG)cells were isolated,cultured,and identified.The ADSCs were then cultured in combination with epidermal growth factor and/or cocultured with hSG cells in a Transwell coculturing system to transform the ADSCs into hSG-like cells.Phenotypic changes of the ADSCs were examined by morphological observation and immunocytochemical analysis of specific markers.Results:The ADSCs showed sweat gland-like morphologic changes and expressed sweat gland markers(cytokeratins 7,14,and 18).Conclusion:These findings revealed that ADSCs can differentiate into hSG-like cells after coculture in a Transwell system and that epidermal growth factor can enhance the efficiency of differentiation.ADSCs may serve as a potential source of cells for sweat gland regeneration.展开更多
文摘HBsAg and HBV DNA in the sweat of HBsAg carriers were detected.Thepositivity rates were 26.1%(12/46)by RIA and 17.4%(8/45)by dot blot hybridization.No HBsAg and HBV DNA were found in 6 persons with negative serum HBsAg.Viralparticles were observed in the sweat by means of immunoelectronic microscope.When se-rum HBeAg or HBV DNA were positive,the positivity rates of HBsAg and HBV DNAin sweat were higher than those with negative serum HBeAg and HBV DNA,and the dif-ference was significant.The results suggested that HBV may be excreted out ofbody through the sweat glands of serum HBsAg carriers,and contaminate environmentalobjects,which may play a role in contact transmission of hepatitis B.No relationshipswere found between anti-HBc,anti-HBe,titer of serum HBsAg and positivity rates ofHBsAg and HBV DNA in sweat.
文摘This paper reports the loss of several major nutrients in the sweat from humanbody during working in hot environment,so as to put forward a rational scheme for sup-plying beverages and foods with appropriate nutrients.Thirteen young male subjectsdressed in gas protection equipment of the whole body were ordered to march in a hotclimatic chamber（DB 34.2℃）at a speed of 5.4 km/h until they subjectively failed to bearfurther but to hold on still for 200 m.The results showed that their maximum tolerancetime was only 58.2±2.9 min,the mean body temperature（MBT）was 37.8±0.1 ℃,heartrate（HR）151.3±8.0 beat/min.Nevertheless,the amount of sweat was only 780.5±75.2 g.Thelevels of K,Na,Ca,Mg,Fe,Cu and Zn in the whole body-sweat of our series were12.3±1.8,113.0±7.0,0.8±0.13,0.9±0.20,0.027±0.004,0.005±0.001 and 0.035±0.002mmol/L,respectively.The concentrations of K and Zn in serum were decreased,and thelevel of Zn was also lower than the normal.The total amount of amino acids in thesweat was 10855±679 μmol/L,of which the amount of essential amino acids was 21.2%.The concentration of lysine was the highest（1038.5±90.7μmol/L）,and that of methioninethe lowest（49.8±4.0μmol/L）in the essential amino acids.In the non-essential aminoacids,the concentrations of serine and glycine were the highest,being 2774.3±179.0 and1572.1±83.9 μmol/L,respectively,and that of cystine the lowest,being 4.8±0.9μmol/L.The amount of vitamins B<sub>1</sub>,B<sub>2</sub> and C in the sweat were only 452±220,312±174 and3330±1200 μg/L,respectively.
基金This study was supported by the National Natural Science Fund of China(Nos.81271711 and 81471796).
文摘Objective:This study was performed to explore the conversion of adipose-derived stem cells(ADSCs)into sweat gland-like cells for the purpose of sweat gland regeneration.Methods:ADSCs and human sweat gland(hSG)cells were isolated,cultured,and identified.The ADSCs were then cultured in combination with epidermal growth factor and/or cocultured with hSG cells in a Transwell coculturing system to transform the ADSCs into hSG-like cells.Phenotypic changes of the ADSCs were examined by morphological observation and immunocytochemical analysis of specific markers.Results:The ADSCs showed sweat gland-like morphologic changes and expressed sweat gland markers(cytokeratins 7,14,and 18).Conclusion:These findings revealed that ADSCs can differentiate into hSG-like cells after coculture in a Transwell system and that epidermal growth factor can enhance the efficiency of differentiation.ADSCs may serve as a potential source of cells for sweat gland regeneration.
