[Objectives]To explore the function of hcp gene in Aeromonas hydrophila.[Methods]A pair of specific primers was designed referring to the hcp gene sequence of A.hydrophila.The hcp gene was amplified by PCR,and perform...[Objectives]To explore the function of hcp gene in Aeromonas hydrophila.[Methods]A pair of specific primers was designed referring to the hcp gene sequence of A.hydrophila.The hcp gene was amplified by PCR,and performed bioinformatics analysis.[Results]The hcp gene had a total length of 1650 bp and encoded 549 amino acids.The theoretical molecular weight of the protein predicted was about 59476.44 kDa.After predicting the N-terminal signal peptide structure of the amino acid sequence,neither obvious signal peptide cleavage site nor signal peptide was found,and the protein had no transmembrane region.The amino acid sequence had a N-glycosylation site,4 protein kinase C phosphorylation sites,7 casein kinase II phosphorylation sites,9 N-myristoylation sites,4 isoprene binding sites,10 microbody C-terminal target signal sites,and an ATP/GTP binding site motif A(P-ring).The amino acid sequence of hcp gene of A.hydrophila was performed homology analysis with other Aeromonas strains,and it showed higher homology with A.veronii.In the secondary structure,theα-helix,β-sheet,random coil and extended strand accounted for 45.36%,6.01%,37.52%and 11.11%,respectively.The tertiary structure model consisted of 18α-helix and 22β-sheet.Analysis of protein-protein network interaction demonstrated that the proteins interacting with Hcp protein were AHA_3407,nrfA,nirB-1,nirB-2 and AHA_1112.[Conclusions]Through the bioinformatics prediction results,the basic information of hcp gene of A.hydrophila is preliminarily understood,and the possible function of this protein is predicted,in order to provide guidance for subsequent vaccine research.展开更多
An intelligent manufacturing system is a composite intelligent system comprising humans,cyber systems,and physical systems with the aim of achieving specific manufacturing goals at an optimized level.This kind of inte...An intelligent manufacturing system is a composite intelligent system comprising humans,cyber systems,and physical systems with the aim of achieving specific manufacturing goals at an optimized level.This kind of intelligent system is called a human-cyber-physical system(HCPS).In terms of technology,HCPSs can both reveal technological principles and form the technological architecture for intelligent manufacturing.It can be concluded that the essence of intelligent manufacturing is to design,construct,and apply HCPSs in various cases and at different levels.With advances in information technology,intelligent manufacturing has passed through the stages of digital manufacturing and digital-networked manufacturing,and is evolving toward new-generation intelligent manufacturing(NGIM).NGIM is characterized by the in-depth integration of new-generation artificial intelligence(AI)technology(i.e.,enabling technology)with advanced manufacturing technology(i.e.,root technology);it is the core driving force of the new industrial revolution.In this study,the evolutionary footprint of intelligent manufacturing is reviewed from the perspective of HCPSs,and the implications,characteristics,technical frame,and key technologies of HCPSs for NGIM are then discussed in depth.Finally,an outlook of the major challenges of HCPSs for NGIM is proposed.展开更多
本文探讨借用国际通行的HACCP危害分析关键控制点的概念建立HCPs清真监控体系(Halal control points)。目的是通过增加这些控制点较好的进行质量控制检测,完善生产过程,生产出合乎标准的清真食品。在简要介绍了清真食品生产的相关规则和...本文探讨借用国际通行的HACCP危害分析关键控制点的概念建立HCPs清真监控体系(Halal control points)。目的是通过增加这些控制点较好的进行质量控制检测,完善生产过程,生产出合乎标准的清真食品。在简要介绍了清真食品生产的相关规则和HCPs应用时应注意的主要问题后,举例介绍了HCPs的应用,以简单易懂的流程图表示。本文对从事食品研发、生产和从事清真食品贸易的专业人士提供了非常有价值的信息。展开更多
Developing high-performing non-noble transition metal catalysts for H_(2) evolution from chemical hydrogen storage materials is of great significance for the hydrogen economy system, yet challenging. Herein,we present...Developing high-performing non-noble transition metal catalysts for H_(2) evolution from chemical hydrogen storage materials is of great significance for the hydrogen economy system, yet challenging. Herein,we present for the first time that anomalous metastable hexagonal close-packed Ni nanoparticles induced by heteroatom N doping encapsulated in carbon(N-hcp-Ni/C) can exhibit admirable catalytic performance for ammonia borane(AB) dehydrogenation, prominently outperforming conventional fcc Ni counterpart with similar morphology and favorably presenting the state-of-the-art level.Comprehensive experimental and theoretical studies unravel that unusual hcp phase engineering of Ni together with N doping could induce charge redistribution and modulate electronic structure, thereby facilitating H_(2)O adsorption and expediting H_(2)O dissociation(rate-determining step). As a result, AB dehydrogenation can be substantially boosted with the assistance of N-hcp-Ni/C. Our proposed strategy highlights that unconventional crystal phase engineering coupled with non-metal heteroatom doping is a promising avenue to construct advanced transition metal catalysts for future renewable energy technologies.展开更多
Based on the Karma model and the Eggleston regularization technique of the strong interfacial energy anisotropy, a phase-field model was established for HCP materials. An explicit finite difference numerical method wa...Based on the Karma model and the Eggleston regularization technique of the strong interfacial energy anisotropy, a phase-field model was established for HCP materials. An explicit finite difference numerical method was used to solve phase field model and simulate the dendrite growth behaviors of HCP materials. Results indicate that the dendrite morphology presents obvious six-fold symmetry, and discontinuity in the variation of interface orientation occurs, resulting in a fact that the corners were formed at the tips of the main stem and side branches. When the interfacial energy anisotropy strength is lower than the critical value（1/35）, the steady-state tip velocity of dendrite increases with anisotropy as expected. As the anisotropy strength crosses the critical value, the steady-state tip velocity drops down by about 0.89%. With further increase in anisotropy strength, the steady-state tip velocity increases and reaches the maximum value at anisotropy strength of 0.04, then decreases.展开更多
基金Supported by Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean UniversityNational Natural Science Foundation of China(32073015)+1 种基金Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2023008)Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).
文摘[Objectives]To explore the function of hcp gene in Aeromonas hydrophila.[Methods]A pair of specific primers was designed referring to the hcp gene sequence of A.hydrophila.The hcp gene was amplified by PCR,and performed bioinformatics analysis.[Results]The hcp gene had a total length of 1650 bp and encoded 549 amino acids.The theoretical molecular weight of the protein predicted was about 59476.44 kDa.After predicting the N-terminal signal peptide structure of the amino acid sequence,neither obvious signal peptide cleavage site nor signal peptide was found,and the protein had no transmembrane region.The amino acid sequence had a N-glycosylation site,4 protein kinase C phosphorylation sites,7 casein kinase II phosphorylation sites,9 N-myristoylation sites,4 isoprene binding sites,10 microbody C-terminal target signal sites,and an ATP/GTP binding site motif A(P-ring).The amino acid sequence of hcp gene of A.hydrophila was performed homology analysis with other Aeromonas strains,and it showed higher homology with A.veronii.In the secondary structure,theα-helix,β-sheet,random coil and extended strand accounted for 45.36%,6.01%,37.52%and 11.11%,respectively.The tertiary structure model consisted of 18α-helix and 22β-sheet.Analysis of protein-protein network interaction demonstrated that the proteins interacting with Hcp protein were AHA_3407,nrfA,nirB-1,nirB-2 and AHA_1112.[Conclusions]Through the bioinformatics prediction results,the basic information of hcp gene of A.hydrophila is preliminarily understood,and the possible function of this protein is predicted,in order to provide guidance for subsequent vaccine research.
文摘An intelligent manufacturing system is a composite intelligent system comprising humans,cyber systems,and physical systems with the aim of achieving specific manufacturing goals at an optimized level.This kind of intelligent system is called a human-cyber-physical system(HCPS).In terms of technology,HCPSs can both reveal technological principles and form the technological architecture for intelligent manufacturing.It can be concluded that the essence of intelligent manufacturing is to design,construct,and apply HCPSs in various cases and at different levels.With advances in information technology,intelligent manufacturing has passed through the stages of digital manufacturing and digital-networked manufacturing,and is evolving toward new-generation intelligent manufacturing(NGIM).NGIM is characterized by the in-depth integration of new-generation artificial intelligence(AI)technology(i.e.,enabling technology)with advanced manufacturing technology(i.e.,root technology);it is the core driving force of the new industrial revolution.In this study,the evolutionary footprint of intelligent manufacturing is reviewed from the perspective of HCPSs,and the implications,characteristics,technical frame,and key technologies of HCPSs for NGIM are then discussed in depth.Finally,an outlook of the major challenges of HCPSs for NGIM is proposed.
文摘本文探讨借用国际通行的HACCP危害分析关键控制点的概念建立HCPs清真监控体系(Halal control points)。目的是通过增加这些控制点较好的进行质量控制检测,完善生产过程,生产出合乎标准的清真食品。在简要介绍了清真食品生产的相关规则和HCPs应用时应注意的主要问题后,举例介绍了HCPs的应用,以简单易懂的流程图表示。本文对从事食品研发、生产和从事清真食品贸易的专业人士提供了非常有价值的信息。
基金financially supported by the National Natural Science Foundation of China(No.51805389)the Key R&D Program of Hubei Province,China(No.2021BAA048)+1 种基金the“111”Project,China(No.B17034)the Fund of the Hubei Key Laboratory of Advanced Technology for Automotive Components,Wuhan University of Technology,China(No.XDQCKF2021011)。
基金supported by the National Natural Science Foundation of China (52002412 and 22072186)the Natural Science Foundation of Guangdong Province (2021A1515010575)the Guangzhou Science and Technology Plan General Project (202102020862)。
文摘Developing high-performing non-noble transition metal catalysts for H_(2) evolution from chemical hydrogen storage materials is of great significance for the hydrogen economy system, yet challenging. Herein,we present for the first time that anomalous metastable hexagonal close-packed Ni nanoparticles induced by heteroatom N doping encapsulated in carbon(N-hcp-Ni/C) can exhibit admirable catalytic performance for ammonia borane(AB) dehydrogenation, prominently outperforming conventional fcc Ni counterpart with similar morphology and favorably presenting the state-of-the-art level.Comprehensive experimental and theoretical studies unravel that unusual hcp phase engineering of Ni together with N doping could induce charge redistribution and modulate electronic structure, thereby facilitating H_(2)O adsorption and expediting H_(2)O dissociation(rate-determining step). As a result, AB dehydrogenation can be substantially boosted with the assistance of N-hcp-Ni/C. Our proposed strategy highlights that unconventional crystal phase engineering coupled with non-metal heteroatom doping is a promising avenue to construct advanced transition metal catalysts for future renewable energy technologies.
基金Project(10834015)supported by the National Natural Science Foundation of ChinaProject(12SKY01-1)supported by the Doctoral Fund of Shangluo University,China
文摘Based on the Karma model and the Eggleston regularization technique of the strong interfacial energy anisotropy, a phase-field model was established for HCP materials. An explicit finite difference numerical method was used to solve phase field model and simulate the dendrite growth behaviors of HCP materials. Results indicate that the dendrite morphology presents obvious six-fold symmetry, and discontinuity in the variation of interface orientation occurs, resulting in a fact that the corners were formed at the tips of the main stem and side branches. When the interfacial energy anisotropy strength is lower than the critical value（1/35）, the steady-state tip velocity of dendrite increases with anisotropy as expected. As the anisotropy strength crosses the critical value, the steady-state tip velocity drops down by about 0.89%. With further increase in anisotropy strength, the steady-state tip velocity increases and reaches the maximum value at anisotropy strength of 0.04, then decreases.
