Human-induced pluripotent stem cell-derived neural stem cell exosomes improve blood-brain barrier function after intracerebral hemorrhage by activating astrocytes via PI3K/AKT/MCP-1 axis

Cerebral edema caused by blood-brain barrier injury after intracerebral hemorrhage is an important factor leading to poor prognosis.Human-induced pluripotent stem cell-derived neural stem cell exosomes(hiPSC-NSC-Exos)have shown potential for brain injury repair in central nervous system diseases.In this study,we explored the impact of hiPSC-NSC-Exos on blood-brain barrier preservation and the underlying mechanism.Our results indicated that intranasal delivery of hiPSC-NSC-Exos mitigated neurological deficits,enhanced blood-brain barrier integrity,and reduced leukocyte infiltration in a mouse model of intracerebral hemorrhage.Additionally,hiPSC-NSC-Exos decreased immune cell infiltration,activated astrocytes,and decreased the secretion of inflammatory cytokines like monocyte chemoattractant protein-1,macrophage inflammatory protein-1α,and tumor necrosis factor-αpost-intracerebral hemorrhage,thereby improving the inflammatory microenvironment.RNA sequencing indicated that hiPSC-NSC-Exo activated the PI3K/AKT signaling pathway in astrocytes and decreased monocyte chemoattractant protein-1 secretion,thereby improving blood-brain barrier integrity.Treatment with the PI3K/AKT inhibitor LY294002 or the monocyte chemoattractant protein-1 neutralizing agent C1142 abolished these effects.In summary,our findings suggest that hiPSC-NSC-Exos maintains blood-brain barrier integrity,in part by downregulating monocyte chemoattractant protein-1 secretion through activation of the PI3K/AKT signaling pathway in astrocytes.

Vertical human-induced vibration of pedestrian bridge

The double degrees-of-freedom（DOFs）parallel model is adopted to analyze static vertical human-induced vibration with the finite element analysis（FEA）method.In the first-order symmetric vibration mode,the periods of the spring-mass model gradually decrease with the increase in K1 and K2,but they are always greater than the period of the add-on mass model.Meanwhile,the periods of the spring-mass model decrease with the decrease in m1 and m2,but they are always greater than the period of the hollow bridge model.Since the human＇s two degrees-of-freedom vibrate in the same direction as that of the bridge mid-span,the existence of human＇s rigidity leads to the reduction in the rigidity of the spring-mass model.In the second-order symmetric vibration mode,the changes of rigidity K2 and mass m2 result in the disappearance or occurrence of some vibration modes.It can be concluded that compared with the spring-mass model,the results of the add-on mass model lean to lack of safety to the structure;besides,the DOF with a smaller ratio of mass to rigidity plays the chief role in the vibration of the structure.

Investigations on serviceability control of long-span structures under human-induced excitation

The increasing strength of new structural materials and the span of new structures, accompanied by aesthetic requirements for greater slenderness, are resulting in more applications of long-span structures. In this paper, serviceability control technology and its design theory are studied. First, a novel tuned mass damper （TMD） with controllable stiffness is developed. Second, methods for modeling human-induced loads are proposed, including standing up, walking, jumping and running, and an analysis method for long-span floor response is proposed based on a finite element model. Third, a design method for long-span floors installed with a multiple TMD （MTMD） system considering human comfort is introduced, largely based on a study of existing literature. Finally, a design, analysis and field test is conducted using several large scale buildings in China including the Beijing Olympic Park National Conference Center, Changsha New Railway Station and the Xi＇an Northern Railway Station. The analytical and field test results show that the MTMD system designed using the proposed method is capable of effectively mitigating the vertical vibration of long-span floor structures. The study presented in this paper provides an important reference for the analysis of vibration serviceability of similar long-span floors and design of control system for these structures.

Integrated Organ-on-a-chip with Human-induced Pluripotent Stem Cells Directional Differentiation for 3D Skin Model Generation

Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device.Unlike the conventional culture dish method,two different epidermal cells are successfully adhered to the front and back sides of the patch,which produces a three-dimensional nanofibrous scaffold similar to a natural extracellular matrix before the patch was cultured in the skin-on-a-chip device to mimic the physiological conditions of human skin.As expected,the differentiated hiPSCs show the expression of keratinocyte-and fibroblast-specific proteins on the patch,and the layering is found between these two kinds of cells,indicating that this approach creates a powerful in vitro system for modeling skin development and diseases.

Human-induced pluripotent stem cell-atrial-specific cardiomyocytes and atrial fibrillation

Patient-specific human-induced pluripotent stem cell-derived atrial cardiomyocytes(hiPSC-aCMs) may be produced,genome-edited,and differentiated into multiple cell types for regenerative medicine,disease modeling,drug testing,toxicity screening,and three-dimensional tissue fabrication.There is presently no complete model of atrial fibrillation(AF) available for studying human pharmacological responses and evaluating the toxicity of potential medication candidates.It has been demonstrated that hiPSC-aCMs can replicate the electrophysiological disease phenotype and genotype of AF.The hiPSC-aCMs,however,are immature and do not reflect the maturity of a CMs in the native myocardium.Numerous laboratories utilize a variety of methodologies and procedures to improve and promote a CM maturation,including electrical stimulation,culture duration,biophysical signals,and changes in metabolic variables.This review covers the current methods being explored for use in the maturation of patient-specific hiPSC-aCMs and their application towards a personalized approach to the pharmacologic therapy of AF.

The Physical Principles Elucidate Numerous Atmospheric Behaviors and Human-Induced Climatic Consequences

The principles that govern the operation of an open and a closed evaporator are relevant for the understanding of the open and “closed” Earth’s atmospheric behaviors, and are thus described. In these greenhouses, the water is included, otherwise the heat and mass balances do not match. It is incorrect to consider the radiation as the only energy transfer factor for an atmospheric warming. Demonstrations show that when the greenhouse effect and the cloud cover increase, the evaporation and the wind naturally decrease. Researchers did not understand why reductions in surface solar radiation and pan evaporation have been simultaneous with increased air temperature, cloudiness and precipitation for the last decades. It is an error to state that the evaporation increases based solely on the water and/or air temperatures increase. Also, researchers did not comprehend why in the last 50 years the clouds and the precipitation increased while the evaporation decreased and they named such understanding as the “evaporation paradox”, while others “found” “the cause” violating the laws of thermodynamics, but more precipitation is naturally conciliatory with less evaporation. The same principle that increases the formation of clouds may cause less rainfall. Several measurements confirm the working principles of greenhouses described in this paper. The hydrological cycle is analyzed and it was also put in form of equation, which analyses have never been done before. The human influence alters the velocity of the natural cycles as well as the atmospheric heat and mass balances, and the evaporation has not been the only source for the cloud formation. It is demonstrated that the Earth’s greenhouse effect has increased in some places and this proof is not based only on temperatures.

Influence of anthropogenic activities on landslide susceptibility:A case study in Solan district,Himachal Pradesh,India

Landslides in the Himalayan region are primarily controlled by natural parameters,including rainfall,seismic activity,and anthropogenic parameters,such as the construction of large-scale projects like road development,tunneling and hydroelectric power projects and climate change.The parameters which are more crucial among these are a matter of scientific study and analysis.This research,taking Solan district,Himachal Pradesh,India,as the study area,aims to assess the impact of anthropogenic activities on landslide susceptibility at a regional scale.Landslide distribution was characterized into two groups,namely Rainfall-Induced Landslide(RIL)and Human-Induced Landslide(HIL)based on triggering factors.Multiple data such as slope angle,aspect,profile curvature,distance to drainage,distance to lineament,lithology,distance to road,normalized difference vegetation index(NDVI)and land use land cover(LULC)have been considered for delineating the landslide susceptibility zonation(LSZ)map.The effect of anthropogenic activities on landslide occurrences has been examined through the distribution of landslides along national highways and land use land cover changes(LULCC).Two sets of LSZ maps with a LULC of time interval covering five years(2017&2021)were prepared to compare the temporal progression of LULC and landslide susceptibility during the five years.The results indicated the significant impact of anthropogenic activities on the landslide susceptibility.LSZ map of the year 2021 shows that 23%area falls into high and very high susceptible classes and 48%area falls into very low and low susceptibility classes.Compared to LSZ map of 2017,high and very high susceptible classes have been increased by 15%,whereas very low and low susceptible classes have been reduced by 7%.The present case study will help to understand the potential driving parameters responsible for HIL and also suggest the inclusion of LULC in landslide susceptibility analysis.The study will demonstrate new opportunities for research that could help decision-makers prepare for future disasters,both in the Indian Himalayan region and other areas.

Soil Drainage as an Active Agent of Recent Soil Evolution: A Review

While research on pedogenesis mainly focuses on long-term soil formation and most often neglects recent soil evolution in response to human practices or climate changes, this article reviews the impact of artificial subsurface drainage on soil evolution. Artificial drainage is considered as an example of the impact of recent changes in water fluxes on soil evolution over time scales of decades to a century. Results from various classical studies on artificial drainage including hydrological and environmental studies are reviewed and collated with rare studies dealing explicitly with soil morphology changes, in response to artificial drainage. We deduce that soil should react to the perturbations associated with subsurface drainage over time scales that do not exceeding a few decades. Subsurface drainage decreases the intensity of erosion and must i) increase the intensity of the lixiviation and eluviation processes, ii) affect iron and manganese dynamics, and iii) induce heterogeneities in soil evolution at the ten meter scale. Such recent soil evolutions can no longer be neglected as they are mostly irreversible and will probably have unknown, but expectable, feedbacks on crucial soil functions such as the sequestration of soil organic matter or the water available capacity.

Complex solutions and novel complex wave localized excitations for the(2+1)-dimensional Boiti-Leon-Pempinelli system

With the help of the symbolic computation system Maple, the Riccati equation mapping approach and a linear variable separation approach, a new family of complex solutions for the （2＋ 1）-dimensional Boiti-Leon-Pempinelli system （BLP） is derived. Based on the derived solitary wave solution, some novel complex wave localized excitations are obtained.

Dynamic characteristics of the coupled system of footbridge and human

The influence of human body on dynamic characteristics of footbridge was analyzed. A re- alistic footbridge was measured during a mass event. A simulation experiment system including a simple beam as object and a shaker as back ground excitation was built. The acceleration responses of beam were measured when person in static and active stated stood on the beam. The dynamic pa- rameters of the structure were identified by the time-domain approach and verified by theoretical and laboratory tests. The results showed that for the human-structure coupled system, nature frequency of the structure decreased and damping increased. Moreover, the increase of damping with passive person was bigger than that with active person.

Depletion of Forest Resources and Wildlife Population with Habitat Complexity: A Mathematical Model

Nowadays, management and regulation of natural resources like agriculture, fisheries, forestry and wildlife is one of the popular topics in research. The evolution of humankind is largely dependent on the quality of the environment and the resources it provides;but numerous human-induced factors, and climate change may drastically alter the conditions of human sustainability. This paper deals with effect of numerous human-induced activities on the depletion of forestry resources and wildlife population with habitat complexity. A nonlinear mathematical model is proposed and analyzed. In modeling process, we assume that the growth rate of wildlife population wholly depends on forestry biomass. It is depleted by human-induced activities. Local stability analysis of the mathematical model along with the persistence of the system is checked by using theory of nonlinear ordinary differential equations and Butler-McGhee lemma. Analytical results obtained are justified numerically through numerical simulation. Important parameters are investigated and variation of variables with change in these parameters is determined.

Production and Characterization of Hot Nuclei in Ar Induced Reactions at 25 MeV/u

Hot nuclei produced in the reactions of 25 MeV/u40Ar with 209Bi andNa1Ag are studied.Theinitial properties of these nuclei :excitation energies,linear momentum transfer and temperatures are char-acterized through the measurements of folding angle of fission fragments,light charged particle energyspectra and intermediate mass fragments.Nuclei with excitation energies as high as～600 MeV and tem-peratures as high as～6 MeV are produced.The spectra of alpha particles detected in coincidence with fis-sion fragments in the case of different average linear momentum transfer are analyzed and the initial tem-peratures of the hot nuclei are determined.A saturation（or limiting）temperature near 6 MeV is foundfor alpha emission from medium mass nuclei having excitation energies higher than 3 MeV/u.This resultprovides evidence for a soft nuclear equation of state at thee high excitation energies and is consistentwith predictions of statistic multifragmentation model calculations.

Geometric structure of generalized controlled Hamiltonian systems and its application

The main purpose of this paper is to provide a systematic geometric frame for generalized controlled Hamiltonian systems. The pseudo-Poisson manifold and the ω-manifold are proposed as the statespace of the generalized controlled Hamiltonian systems. A Lie group, calledN-group, and its Lie algebra, calledN-algebra, are introduced for the structure analysis of the systems. Some properties, including spectrum, structure-preservation, etc. are investigated. As an example the theoretical results are applied to power systems. The stabilization of excitation systems is investigated.

The road to opportunities： landscape change promotes body-size divergence in a highly mobile species

Landscape change provides a suitable framework for investigating population-level responses to novel ecological pressures. However, relatively little attention has been paid to examine the poten- tial influence of landscape change on the geographic scale of population differentiation. Here, we tested for morphological differentiation of red-necked nightjars Caprimulgus ruficollis breeding in a managed property and a natural reserve situated less than 10 km apart. At both sites, we also esti- mated site fidelity over 5 years and quantified the potential foraging opportunities for nightjars. Breeding birds in the managed habitat were significantly larger in size--as indexed by keel length--than those in the natural one. However, there were no significant differences in wing or tail length. Immigration from neighboring areas was almost negligible and, furthermore, no individual （out of 1130 captures overall） exchanged habitats between years, indicating strong site fidelity. Food supply for nightjars was equally abundant in both habitats, but the availability of foraging sites was remarkably higher in the managed property. As a result, nightjars--particularly fledg- lings-in the latter habitat benefited from increased foraging opportunities in relation to those in the natural site. It seems likely that the fine-scale variation in nightjar morphology reflects a pheno- typic response to unequal local conditions, since non-random dispersal or differential mortality had been determined not to be influential. High site fidelity appears to contribute to the mainten- ance of body-size differences between the two habitats. Results from this nightjar population high- light the potential of human-induced landscape change to promote population-level responses at exceedingly small geographic scales.