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Nuclear transfer of goat somatic cells transgenic for human lactoferrin gene
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作者 Lan LI Wei SHEN +4 位作者 Lingjiang MIN Qingyu PAN Yujiang SUN Jixian DENG Qingjie PAN 《Frontiers in Biology》 CSCD 2008年第3期269-274,共6页
Transgenic animal mammary gland bioreactors are used to produce recombinant proteins with appropriate post-translational modifications.The nuclear transfer of transgenic somatic cells is a powerful method to produce m... Transgenic animal mammary gland bioreactors are used to produce recombinant proteins with appropriate post-translational modifications.The nuclear transfer of transgenic somatic cells is a powerful method to produce mammary gland bioreactors.We established an efficient gene transfer and nuclear transfer approach in goat somatic cells.Gene targeting vector pGBC2LF was constructed by cloning human lactoferrin(LF)gene cDNA into exon 2 of the milk goat beta-casein gene and the endogenous start codon was replaced by that of human LF gene.Goat fetal fibroblasts were transfected with lin-earized pGBC2LF and 14 cell lines were positive accord-ing to PCR and Southern blot.The transgenic cells were used as donor cells of nuclear transfer and some of recon-structed embryos could develop into blastocyst in vitro. 展开更多
关键词 humanlactoferrin GENETRANSFER nucleartransfer GOAT mammary gland bioreactor
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