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Human Leukocyte Antigen-A Allele Distribution in Nasopharyngeal Carcinoma Patients Showing Anti-Melanoma-Associated Antigen A or Synovial Sarcoma X-2 T Cell Response in Blood 被引量:2
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作者 Pei-Wen Fan Li Huang +5 位作者 Xue-Mei Chang Ya-Ning Feng Xuan Yao Yan-Chun Peng Tao Dong Ruo-Zheng Wang 《Chinese Medical Journal》 SCIE CAS CSCD 2018年第11期1289-1295,共7页
Background: Development of innovative immunotherapy is imperative to improve the poor survival of the nasopharyngeal carcinoma (NPC) patients. In this study, we evaluated the T cell response to melanoma-associated ... Background: Development of innovative immunotherapy is imperative to improve the poor survival of the nasopharyngeal carcinoma (NPC) patients. In this study, we evaluated the T cell response to melanoma-associated antigen (MAGE)-A1, MAGE-A3, or synovial sarcoma X-2 (SSX-2) in the peripheral blood of treatment-naive NPC patients. The relationship of responses among the three proteins and the human leukocyte antigen (HLA)-A types were analyzed to provide evidence of designing novel therapy. Methods: Sixty-one NPC patients admitted into the Tumor Hospital affiliated to the Xinjiang Medical University between March 2015 and July 2016 were enrolled. Mononuclear cells were isolated from the peripheral blood before any treatment. HLA-A alleles were typed with Sanger sequence-based typing technique. The T cell response to the MAGE-A1, MAGE-A3, or SSX-2 was evaluated with the Enzyme-Linked ImmunoSpot assay. Mann-Whitney U-test was used to compare the T cell responses from different groups. Spearman's rank correlation was used to analyze the relationship of T cell responses. Results: HLA-A*02:01, A*02:07, and A*24:02 were the three most frequent alleles (18.9%, 12.3%, and 11.5%, respectively) among the 22 detected alleles. 31.1%, 19.7%, and 16.4% of the patients displayed MAGE-A1, MAGE-A3, or SSX-2-specific T cell response, respectively. The magnitudes of response to the three proteins were 32.5, 38.0, and 28.7 SFC/106 peripheral blood mononuclear cells, respectively. The T cell response against the three proteins correlated with each other to different extent. The percentage of A*02:01 and A*24:02 carriers were significantly higher in patients responding to any of the three proteins compared to the nonresponders. Conclusion: MAGE-A1, MAGE-A3, or SSX-2-specific T cell responses were detectable in a subgroup of NPC patients, the frequency and magnitude of which were correlated. 展开更多
关键词 Human Leukocyte antigen-a Melanoma-Associated antigen-a Nasopharyngeal Carcinoma Synovial Sarcoma X-2 T Cell Response
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重组腺相关病毒介导HLA-A2基因转导肝癌细胞增强杀伤性T细胞抗肿瘤免疫应答 被引量:3
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作者 周军 马萍 +1 位作者 李钧 宋伟 《中华生物医学工程杂志》 CAS 2017年第2期117-121,共5页
目的 本研究旨在探讨重组腺相关病毒(rAAV)介导HLA-A2基因转导肝癌细胞增强杀伤性T细胞(CTL)抗肝癌免疫应答.方法 利用携带人类甲胎蛋白(AFP)基因的rAAV(rAAV-AFP)转导人树突状细胞(DC)体外诱导AFP特异性抗肝癌CTL免疫应答.... 目的 本研究旨在探讨重组腺相关病毒(rAAV)介导HLA-A2基因转导肝癌细胞增强杀伤性T细胞(CTL)抗肝癌免疫应答.方法 利用携带人类甲胎蛋白(AFP)基因的rAAV(rAAV-AFP)转导人树突状细胞(DC)体外诱导AFP特异性抗肝癌CTL免疫应答.利用携带人类HLA-A2基因的rAAV(rAAV-HLA-A2)体外转导肝癌细胞提高HLA-A2分子表达水平,增强肝癌细胞对rAAV-AFP转导DC疫苗诱导的AFP特异性CTL免疫反应的敏感性.结果 rAAV-AFP转导DC疫苗能够有效诱导AFP特异性抗肝癌CTL免疫应答.rAAV-HLA-A2能够提高肝癌细胞HLA-A2分子表达,并增强肝癌细胞对rAAV-AFP转导DC疫苗诱导的CTL免疫反应的敏感性.结论 通过rAAV-HLA-A2提高肝癌细胞HLA-A2分子表达是增强CTL抗肝癌免疫应答的有效途径.为AFP表达阳性但HLA-class I分子表达低下的肝癌患者的主动性免疫治疗提供了新的解决方案. 展开更多
关键词 腺病毒科 甲胎蛋白类 树突细胞 肝肿瘤 人类白细胞抗原-A2 human leukocyte antigen-a2
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